##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211447_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2733029 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06227120165941 33.0 31.0 34.0 30.0 34.0 2 32.186122430460856 33.0 31.0 34.0 30.0 34.0 3 32.26513513028951 34.0 31.0 34.0 30.0 34.0 4 35.90978434550091 37.0 35.0 37.0 35.0 37.0 5 35.776014085470734 37.0 35.0 37.0 35.0 37.0 6 35.81743808792369 37.0 35.0 37.0 35.0 37.0 7 35.75067516663746 37.0 35.0 37.0 33.0 37.0 8 35.73829659326703 37.0 35.0 37.0 33.0 37.0 9 37.321010131981765 39.0 37.0 39.0 34.0 39.0 10-11 37.30685495836305 39.0 37.0 39.0 34.0 39.0 12-13 37.228617222868834 39.0 37.0 39.0 33.0 39.0 14-15 38.43057153802612 40.0 38.0 41.0 33.5 41.0 16-17 38.42314260112132 40.0 38.0 41.0 34.0 41.0 18-19 38.44288534808815 40.0 38.0 41.0 34.0 41.0 20-21 38.404477230208684 40.0 38.0 41.0 34.0 41.0 22-23 38.28938258613428 40.0 38.0 41.0 34.0 41.0 24-25 38.17368897293076 40.0 37.5 41.0 33.0 41.0 26-27 38.038197911547954 40.0 37.0 41.0 33.0 41.0 28-29 37.95357897775691 40.0 37.0 41.0 33.0 41.0 30-31 37.973128349534534 40.0 37.0 41.0 33.0 41.0 32-33 37.783925637086185 40.0 37.0 41.0 33.0 41.0 34-35 37.65094259885278 40.0 37.0 41.0 33.0 41.0 36-37 37.48215606201032 40.0 36.0 41.0 32.0 41.0 38-39 37.35109085926274 40.0 36.0 41.0 32.0 41.0 40-41 37.26952348474897 40.0 36.0 41.0 32.0 41.0 42-43 37.11709608643011 40.0 36.0 41.0 31.0 41.0 44-45 36.94619925364861 39.0 36.0 41.0 31.0 41.0 46-47 36.70020094920325 39.0 35.0 41.0 30.0 41.0 48-49 36.461756900493924 39.0 35.0 41.0 30.0 41.0 50-51 36.27559971006528 39.0 35.0 41.0 29.0 41.0 52-53 36.07004060330132 39.0 35.0 40.0 28.5 41.0 54-55 35.87458493854255 39.0 35.0 40.0 28.0 41.0 56-57 35.64514664864515 38.0 35.0 40.0 27.5 41.0 58-59 35.38372077281288 38.0 34.0 40.0 27.0 41.0 60-61 35.13267074736492 38.0 34.0 40.0 26.0 41.0 62-63 34.84064622073165 37.0 34.0 40.0 26.0 41.0 64-65 34.503463739316345 37.0 33.5 40.0 26.0 41.0 66-67 34.150820390123926 36.0 33.0 39.0 25.5 41.0 68-69 33.77230958764068 36.0 33.0 39.0 25.0 40.0 70-71 33.38791593503033 35.0 32.5 38.5 24.0 40.0 72-73 33.01791345792525 35.0 32.0 37.5 23.5 40.0 74-75 32.63093970096915 35.0 32.0 37.0 23.0 39.0 76-77 31.57642180159815 34.0 30.5 36.0 21.5 39.0 78-79 32.0222890060808 35.0 31.0 36.0 23.0 38.5 80-81 31.876498017401204 35.0 31.0 36.0 22.5 37.5 82-83 31.57731677929506 34.0 31.0 35.0 21.0 37.0 84-85 31.289881300198424 34.0 31.0 35.0 20.0 36.5 86-87 31.011022751679548 34.0 30.5 35.0 20.0 36.0 88-89 30.750900557586473 34.0 30.0 35.0 19.5 36.0 90-91 30.50590791389334 34.0 30.0 35.0 18.5 35.0 92-93 30.312895143081175 34.0 30.0 35.0 18.0 35.0 94-95 30.129470452014964 34.0 29.0 35.0 18.0 35.0 96-97 29.944804098309973 34.0 29.0 35.0 17.5 35.0 98-99 29.729490978690677 34.0 29.0 35.0 17.0 35.0 100 27.921447595323723 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.24692500418877472 1101 2 -0.2525401924811703 1101 3 -0.3248121801395527 1101 4 -0.20920257072636161 1101 5 -0.27745951909299293 1101 6 -0.24556170423277734 1101 7 -0.34211615379120985 1101 8 -0.25472371071464295 1101 9 -0.5102418397243085 1101 10-11 -0.49362678477468336 1101 12-13 -0.47871786910945247 1101 14-15 -0.6424807769788003 1101 16-17 -0.6110669740235721 1101 18-19 -0.6439054173275522 1101 20-21 -0.6196706957168914 1101 22-23 -0.6677884214412941 1101 24-25 -0.5940516788507821 1101 26-27 -0.8053601029235722 1101 28-29 -0.708786526878697 1101 30-31 -0.6564457902591982 1101 32-33 -0.4857886907246609 1101 34-35 -0.6600157319731608 1101 36-37 -0.6653238464474001 1101 38-39 -0.6904198310538661 1101 40-41 -0.7802997127302689 1101 42-43 -0.779827313037778 1101 44-45 -0.711173120644844 1101 46-47 -0.6998340482270322 1101 48-49 -0.625895718356297 1101 50-51 -0.6296099344641632 1101 52-53 -0.774840842155399 1101 54-55 -0.7473684166977819 1101 56-57 -0.964159928132311 1101 58-59 -0.7790396225392513 1101 60-61 -0.8027864860215388 1101 62-63 -0.7830544965144739 1101 64-65 -0.7785328021910445 1101 66-67 -0.8934148721479076 1101 68-69 -0.9221067062024275 1101 70-71 -0.908470908626434 1101 72-73 -0.8923263718090695 1101 74-75 -1.0004213942234017 1101 76-77 -0.8123619262228878 1101 78-79 -0.7049104847957395 1101 80-81 -0.5364433897694916 1101 82-83 -0.7769747123445931 1101 84-85 -0.8654964999818127 1101 86-87 -1.0271654158590948 1101 88-89 -1.2316854221383693 1101 90-91 -1.0847830246441035 1101 92-93 -1.3587440025916386 1101 94-95 -1.5453362793918721 1101 96-97 -1.4085118491650768 1101 98-99 -1.5636828875627913 1101 100 -1.6172590208913924 1102 1 -0.2426513763949174 1102 2 -0.18106531604475506 1102 3 -0.24171489420288594 1102 4 -0.10806073445654363 1102 5 -0.15277453569889587 1102 6 -0.16683817698272208 1102 7 -0.1916631217176672 1102 8 -0.2942262081373883 1102 9 -0.4812613442587619 1102 10-11 -0.33982418508767154 1102 12-13 -0.4170298411856592 1102 14-15 -0.5491503546672902 1102 16-17 -0.5332623455676782 1102 18-19 -0.6412022273155955 1102 20-21 -0.700566478382612 1102 22-23 -0.6767532331745727 1102 24-25 -0.7585600258445879 1102 26-27 -0.7778774417403653 1102 28-29 -0.7310561879751916 1102 30-31 -0.653921012164389 1102 32-33 -0.6716006593599531 1102 34-35 -0.5576485826136519 1102 36-37 -0.43415086293217797 1102 38-39 -0.5621230816260905 1102 40-41 -0.7439399833796045 1102 42-43 -0.6704270867626079 1102 44-45 -0.6466114049174791 1102 46-47 -0.7694298359853278 1102 48-49 -0.8774635754450841 1102 50-51 -0.7900072373301796 1102 52-53 -0.670847167950356 1102 54-55 -0.7715457261428398 1102 56-57 -0.7814540817268991 1102 58-59 -0.8502617630968743 1102 60-61 -0.8703535049012032 1102 62-63 -0.8331087598883187 1102 64-65 -0.7634813397294593 1102 66-67 -0.8538672986188018 1102 68-69 -0.9715968300619409 1102 70-71 -0.9146251735142812 1102 72-73 -1.113073527197077 1102 74-75 -1.027912829763192 1102 76-77 -0.7003913296079283 1102 78-79 -0.6587996925435959 1102 80-81 -0.7009042520100266 1102 82-83 -0.7732994612603612 1102 84-85 -0.9024355089130758 1102 86-87 -0.827948639014032 1102 88-89 -0.9286158769321986 1102 90-91 -1.0678141072001353 1102 92-93 -1.0795806220075335 1102 94-95 -0.9855290606082825 1102 96-97 -1.210722195611087 1102 98-99 -1.2386375361824946 1102 100 -1.210581357031142 1103 1 -0.3499662194201534 1103 2 -0.25935642093931577 1103 3 -0.34698661870775993 1103 4 -0.21172036151133256 1103 5 -0.1738458410481698 1103 6 -0.30213775970781853 1103 7 -0.19666354743860381 1103 8 -0.31280542180860493 1103 9 -0.3838321173713908 1103 10-11 -0.561790376348867 1103 12-13 -0.6623992284655245 1103 14-15 -0.6594207096843547 1103 16-17 -0.7221631854176209 1103 18-19 -0.6406740085148641 1103 20-21 -0.6463312561135481 1103 22-23 -0.8143109320383957 1103 24-25 -0.7513432867027845 1103 26-27 -0.803551747789605 1103 28-29 -0.8939089764130941 1103 30-31 -0.6957881010641529 1103 32-33 -0.6710507270820685 1103 34-35 -0.5867655861127687 1103 36-37 -0.6521598157236568 1103 38-39 -0.6418924981764107 1103 40-41 -0.7835068114782757 1103 42-43 -0.8085395906137549 1103 44-45 -0.7055854653479727 1103 46-47 -0.7836870418677293 1103 48-49 -0.912215739184532 1103 50-51 -0.8776839499849629 1103 52-53 -0.8396483378109565 1103 54-55 -0.8022066946459958 1103 56-57 -0.8322723378713874 1103 58-59 -1.049863414825687 1103 60-61 -1.0394007893316868 1103 62-63 -1.0404952060965158 1103 64-65 -0.9998184334267037 1103 66-67 -0.9525223111322632 1103 68-69 -0.9871285486648489 1103 70-71 -0.9691757541189858 1103 72-73 -0.8114263975306883 1103 74-75 -0.8250718520890921 1103 76-77 -0.8168441270731641 1103 78-79 -0.6633726876117336 1103 80-81 -0.725242409838625 1103 82-83 -0.692128927030172 1103 84-85 -0.8107157297134897 1103 86-87 -0.7767456097959027 1103 88-89 -0.7510265618778504 1103 90-91 -0.985029680311122 1103 92-93 -0.9486564759028475 1103 94-95 -0.8958667615989953 1103 96-97 -1.242145529426562 1103 98-99 -1.2099999389035503 1103 100 -1.3493981811119689 1104 1 -0.29624817886245935 1104 2 -0.25697039400179733 1104 3 -0.25981969601411237 1104 4 -0.22925878444425507 1104 5 -0.1370855233579391 1104 6 -0.2330920949392663 1104 7 -0.263707800527925 1104 8 -0.19629885822276805 1104 9 -0.31978316645795246 1104 10-11 -0.29905635964191646 1104 12-13 -0.39134351377581567 1104 14-15 -0.5500227951464609 1104 16-17 -0.5549178714996401 1104 18-19 -0.5004510367691495 1104 20-21 -0.4656990484778021 1104 22-23 -0.5039279787384388 1104 24-25 -0.6912201375610678 1104 26-27 -0.731946798569659 1104 28-29 -0.6799254363695866 1104 30-31 -0.6370897691645041 1104 32-33 -0.4956535239674409 1104 34-35 -0.6091887213610292 1104 36-37 -0.5020525438676771 1104 38-39 -0.6472671761719866 1104 40-41 -0.6617301813057139 1104 42-43 -0.6166559907594618 1104 44-45 -0.6520475797596745 1104 46-47 -0.5760723166541482 1104 48-49 -0.5622925472785951 1104 50-51 -0.622527362964199 1104 52-53 -0.4739708648021761 1104 54-55 -0.45975599256713195 1104 56-57 -0.50755170945407 1104 58-59 -0.580322906890494 1104 60-61 -0.6111997705446512 1104 62-63 -0.7061137879730879 1104 64-65 -0.6457495422977857 1104 66-67 -0.7686354898377772 1104 68-69 -0.668006026534691 1104 70-71 -0.7385042309867274 1104 72-73 -0.7072228417938291 1104 74-75 -0.6899693378351728 1104 76-77 -0.47775330696241625 1104 78-79 -0.4531336447946259 1104 80-81 -0.525337643992458 1104 82-83 -0.5714991630643844 1104 84-85 -0.3285279159408496 1104 86-87 -0.3862345050567235 1104 88-89 -0.5519986761220501 1104 90-91 -0.38713072737771625 1104 92-93 -0.7682733977917913 1104 94-95 -0.7832713837481684 1104 96-97 -0.7664669429713875 1104 98-99 -0.8031614894646921 1104 100 -0.7836688786516461 1105 1 -0.3190329417951432 1105 2 -0.27780663679239836 1105 3 -0.2909549435965495 1105 4 -0.14423133075280958 1105 5 -0.21919414632576917 1105 6 -0.2685066933899307 1105 7 -0.2917428568286837 1105 8 -0.3496727017332546 1105 9 -0.3839039184660322 1105 10-11 -0.4755505147134116 1105 12-13 -0.47097817651785334 1105 14-15 -0.5562179643836629 1105 16-17 -0.4985385928596813 1105 18-19 -0.5104877954578626 1105 20-21 -0.7161394955473739 1105 22-23 -0.744190749333761 1105 24-25 -0.7242729412878717 1105 26-27 -0.7066010286624902 1105 28-29 -0.6666439350833144 1105 30-31 -0.6552275627458641 1105 32-33 -0.6030241876649285 1105 34-35 -0.6193242172873781 1105 36-37 -0.7386585611203103 1105 38-39 -0.5921484166023703 1105 40-41 -0.5984937989875476 1105 42-43 -0.6525792834583299 1105 44-45 -0.687034211527596 1105 46-47 -0.7728888069418787 1105 48-49 -0.6974988970466498 1105 50-51 -0.6544539302638199 1105 52-53 -0.6199319904509224 1105 54-55 -0.6759219798162874 1105 56-57 -0.6508600419922388 1105 58-59 -0.713970314161223 1105 60-61 -0.6527133286681917 1105 62-63 -0.6487951627493089 1105 64-65 -0.6893929811214647 1105 66-67 -0.7536729278386929 1105 68-69 -0.8777972217639558 1105 70-71 -1.012517127175819 1105 72-73 -1.0269196410643424 1105 74-75 -0.9251573303760097 1105 76-77 -0.8954892320876802 1105 78-79 -0.7582949008713129 1105 80-81 -0.6471384785037948 1105 82-83 -0.5879625310752914 1105 84-85 -0.569320866007299 1105 86-87 -0.5776466718856881 1105 88-89 -0.6865811645279294 1105 90-91 -0.5239930191053368 1105 92-93 -0.6233974338793793 1105 94-95 -0.6944748532325171 1105 96-97 -0.6701715957227918 1105 98-99 -0.7792149446992838 1105 100 -0.8606896112598328 1106 1 -0.3389939595330098 1106 2 -0.2920538075231107 1106 3 -0.3337782433322296 1106 4 -0.19076530940343872 1106 5 -0.17496055984797465 1106 6 -0.24303144038909608 1106 7 -0.13976060566572102 1106 8 -0.23012485210325906 1106 9 -0.43709512113462523 1106 10-11 -0.38923324171692997 1106 12-13 -0.4038193222328559 1106 14-15 -0.5944184660766041 1106 16-17 -0.5760642533097666 1106 18-19 -0.5284515989860097 1106 20-21 -0.5641432214243594 1106 22-23 -0.5306489569957833 1106 24-25 -0.57514181642712 1106 26-27 -0.5728696991762021 1106 28-29 -0.6190232945395735 1106 30-31 -0.5699124221793994 1106 32-33 -0.4133644363805402 1106 34-35 -0.3848987904488368 1106 36-37 -0.5021082249609705 1106 38-39 -0.510926883602977 1106 40-41 -0.5061447729957251 1106 42-43 -0.46746633987450537 1106 44-45 -0.4294032143619617 1106 46-47 -0.3858559712800016 1106 48-49 -0.3406774712171412 1106 50-51 -0.5041396279413348 1106 52-53 -0.5225593761167886 1106 54-55 -0.601801705122945 1106 56-57 -0.5549460505250963 1106 58-59 -0.6139348258880162 1106 60-61 -0.45360335575835364 1106 62-63 -0.5387548016933366 1106 64-65 -0.5301869470182439 1106 66-67 -0.4449445361233657 1106 68-69 -0.5080774929476348 1106 70-71 -0.4371705877133252 1106 72-73 -0.3986214643625132 1106 74-75 -0.4800705368707554 1106 76-77 -0.42832309213070374 1106 78-79 -0.2375742930739193 1106 80-81 -0.1526921974084665 1106 82-83 -0.3028795135849798 1106 84-85 -0.22053686816322582 1106 86-87 -0.2396580814046949 1106 88-89 -0.4622299418668234 1106 90-91 -0.4954910002691335 1106 92-93 -0.37707317290504605 1106 94-95 -0.2684212902255574 1106 96-97 -0.3377755027231508 1106 98-99 -0.522265510396462 1106 100 -0.7240868652665604 1107 1 -0.4033862271313424 1107 2 -0.3189680765825287 1107 3 -0.38611919879181045 1107 4 -0.21887115866930174 1107 5 -0.2310950403485279 1107 6 -0.37803354117377097 1107 7 -0.36607605646382524 1107 8 -0.38365625220999533 1107 9 -0.5549555760396743 1107 10-11 -0.5320672948686749 1107 12-13 -0.5088559061677245 1107 14-15 -0.6545171840301691 1107 16-17 -0.6133866802270589 1107 18-19 -0.8088900762767111 1107 20-21 -0.6851476614611514 1107 22-23 -0.6584251155501377 1107 24-25 -0.7728248969723381 1107 26-27 -0.751702874162099 1107 28-29 -0.7814571008964322 1107 30-31 -0.738065283599525 1107 32-33 -0.7717946392292845 1107 34-35 -0.7575151568862495 1107 36-37 -0.7093152412032495 1107 38-39 -0.6852643793919455 1107 40-41 -0.6626778348042137 1107 42-43 -0.8128675140428001 1107 44-45 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0.7403961346172281 2115 48-49 0.6963223222228478 2115 50-51 0.7624288132708514 2115 52-53 0.778507810810467 2115 54-55 0.7668479774552068 2115 56-57 0.6204248321547894 2115 58-59 0.5697966628126707 2115 60-61 0.6148480053288452 2115 62-63 0.6332415709695809 2115 64-65 0.6673805401971222 2115 66-67 0.716178242908768 2115 68-69 0.7871110318676244 2115 70-71 0.8992343606526418 2115 72-73 0.797796629816915 2115 74-75 0.4243763539234777 2115 76-77 0.41179002634041595 2115 78-79 0.3228633753530801 2115 80-81 0.508679647626149 2115 82-83 0.5537581670207068 2115 84-85 0.730151048314891 2115 86-87 0.7423144831274016 2115 88-89 0.7010110324755559 2115 90-91 0.8211450803919575 2115 92-93 0.9313086861111799 2115 94-95 0.9938842538212747 2115 96-97 0.9791769251927782 2115 98-99 1.0540436084519627 2115 100 1.026648691352971 2116 1 0.1526937370433501 2116 2 0.2983833316759643 2116 3 0.3365466911871451 2116 4 0.20721928606318585 2116 5 0.35374655198731375 2116 6 0.27499004657993 2116 7 0.3971840941395399 2116 8 0.4605820995421013 2116 9 0.5701619609938007 2116 10-11 0.5878384982685105 2116 12-13 0.4392770893931086 2116 14-15 0.5857090164190595 2116 16-17 0.7508394067911084 2116 18-19 0.6317279930556623 2116 20-21 0.7497371764683081 2116 22-23 0.7624004735529155 2116 24-25 0.8780526577374701 2116 26-27 0.8517075607869558 2116 28-29 0.8439739666907613 2116 30-31 0.6225490874074922 2116 32-33 0.6759070819689796 2116 34-35 0.6868954682961643 2116 36-37 0.6526762538300019 2116 38-39 0.569354273637785 2116 40-41 0.7946626320827761 2116 42-43 0.8043285763664798 2116 44-45 0.8329816790101177 2116 46-47 0.8005245969638963 2116 48-49 0.7359464542251999 2116 50-51 0.8503133533248075 2116 52-53 0.8317888241971332 2116 54-55 0.8311822985972839 2116 56-57 0.8345741208542279 2116 58-59 0.8342558049136315 2116 60-61 0.9025673039013995 2116 62-63 0.8193848791461491 2116 64-65 0.6978479050511268 2116 66-67 0.7674858440169103 2116 68-69 0.7219622437192399 2116 70-71 0.508325325650226 2116 72-73 0.7048786258937625 2116 74-75 0.6617725678492263 2116 76-77 0.5002146248280326 2116 78-79 0.6072128993823327 2116 80-81 0.5453818175130998 2116 82-83 0.6746053729730512 2116 84-85 0.5400713514930331 2116 86-87 0.5100630805222224 2116 88-89 0.6056611815786468 2116 90-91 0.48376015961357055 2116 92-93 0.6354960584343239 2116 94-95 0.7715192004099052 2116 96-97 0.8871518439697077 2116 98-99 1.0238453410369388 2116 100 0.5856581816965836 2201 1 0.6617040225323407 2201 2 0.6011083364353738 2201 3 0.5429251728583537 2201 4 0.40965510956404927 2201 5 0.48900754840672533 2201 6 0.47282365130222104 2201 7 0.5370318089677113 2201 8 0.508619136682178 2201 9 0.7035104502442877 2201 10-11 0.5708967927747395 2201 12-13 0.6091154364336688 2201 14-15 0.7393828624065932 2201 16-17 0.6430224113112573 2201 18-19 0.6658070465315404 2201 20-21 0.7447770581249742 2201 22-23 0.7435170486547875 2201 24-25 0.7267982605011483 2201 26-27 0.7380577562704715 2201 28-29 0.784727181328357 2201 30-31 0.951596999050615 2201 32-33 0.7825438633927035 2201 34-35 0.9582403717944317 2201 36-37 0.9119446800933488 2201 38-39 0.8910217239342657 2201 40-41 0.8646305065727091 2201 42-43 0.017711536054790145 2201 44-45 0.8440321509963766 2201 46-47 0.9324552991823154 2201 48-49 0.9623274676234814 2201 50-51 0.9660505409774558 2201 52-53 0.9930634156332161 2201 54-55 0.8953967537652474 2201 56-57 0.975780502714187 2201 58-59 0.9453011498544939 2201 60-61 0.9164557346240869 2201 62-63 0.917266535014889 2201 64-65 0.9257597786921181 2201 66-67 0.9897746604205935 2201 68-69 0.9440226283907265 2201 70-71 1.0029607072713134 2201 72-73 1.0486454303812351 2201 74-75 1.0769448067663632 2201 76-77 1.1893140692391349 2201 78-79 0.8531097726397832 2201 80-81 0.8267694702364601 2201 82-83 0.7056258855265618 2201 84-85 0.5389125001260773 2201 86-87 0.5396646137075223 2201 88-89 0.6187254099444033 2201 90-91 -0.12339290335886943 2201 92-93 0.11916890285949577 2201 94-95 0.42258222769572384 2201 96-97 0.3756323864349582 2201 98-99 0.3143476086615564 2201 100 0.5084478587682391 2202 1 0.668412844280752 2202 2 0.6076652317083955 2202 3 0.5983035651424871 2202 4 0.36028767419131924 2202 5 0.3337324054419639 2202 6 0.4301503001596032 2202 7 0.49658880783682235 2202 8 0.5254407134492567 2202 9 0.6326676332456884 2202 10-11 0.6388087941860761 2202 12-13 0.6736318486108459 2202 14-15 0.8930757810723975 2202 16-17 0.7962277998680918 2202 18-19 0.8258093568542577 2202 20-21 0.7777396850789415 2202 22-23 0.7643242572084503 2202 24-25 0.780917353519591 2202 26-27 0.7676939302893473 2202 28-29 0.6983878638401677 2202 30-31 0.8935587014420392 2202 32-33 0.5289150746043134 2202 34-35 0.794422236237871 2202 36-37 0.8295743063195431 2202 38-39 0.9676806591832374 2202 40-41 0.8782304184943612 2202 42-43 1.0194442780957687 2202 44-45 0.9714220671529077 2202 46-47 1.052814660562305 2202 48-49 1.0585239092063432 2202 50-51 1.1117442381309885 2202 52-53 1.1092874943974493 2202 54-55 0.9727137842246663 2202 56-57 0.9753912588485676 2202 58-59 0.9874871876163454 2202 60-61 0.9877713442133569 2202 62-63 1.0261097178507086 2202 64-65 0.880825085403707 2202 66-67 0.9049507065785036 2202 68-69 0.9093621526376268 2202 70-71 0.9859444002073161 2202 72-73 0.9253000960844702 2202 74-75 0.9392761217821217 2202 76-77 0.9884546985398295 2202 78-79 0.9020650993207084 2202 80-81 0.7116244812310804 2202 82-83 0.7701502082834146 2202 84-85 0.8177113447046445 2202 86-87 0.5977490744248826 2202 88-89 0.5687314133158239 2202 90-91 0.6569952091854923 2202 92-93 0.5905866781270781 2202 94-95 0.4645372331854496 2202 96-97 0.36957732533277365 2202 98-99 0.23893041769322565 2202 100 0.5011983001352398 2203 1 0.6122701652065885 2203 2 0.5561048472335344 2203 3 0.5337970623092474 2203 4 0.31399304444627774 2203 5 0.47958342411768484 2203 6 0.43766119791235525 2203 7 0.48862525486497077 2203 8 0.4305727311722123 2203 9 0.5994666489556479 2203 10-11 0.5739650385894493 2203 12-13 0.6012071254871927 2203 14-15 0.7828549068165245 2203 16-17 0.7125901219401172 2203 18-19 0.6913401991959205 2203 20-21 0.7144486577709799 2203 22-23 0.6647825314413964 2203 24-25 0.7139483346576156 2203 26-27 0.7190158088935803 2203 28-29 0.7626212512593042 2203 30-31 0.8624549972806506 2203 32-33 0.8897693751423006 2203 34-35 1.026001720882519 2203 36-37 0.9583388217322053 2203 38-39 1.0559371217207598 2203 40-41 0.9032684979467689 2203 42-43 1.025606948322789 2203 44-45 0.9866574299576314 2203 46-47 1.0351623382753772 2203 48-49 1.0591651707667395 2203 50-51 0.9621653059853941 2203 52-53 0.9055655581255735 2203 54-55 0.8729830043861355 2203 56-57 0.9645680531720657 2203 58-59 1.017078906936085 2203 60-61 0.9830335753066066 2203 62-63 0.9997422155404223 2203 64-65 1.0752725147160689 2203 66-67 1.0668408756772365 2203 68-69 0.9435513893830176 2203 70-71 1.027466338091024 2203 72-73 1.0344825721040465 2203 74-75 0.9939354707466066 2203 76-77 1.0780908416487343 2203 78-79 0.9287120972002612 2203 80-81 0.8090154231121254 2203 82-83 0.7690163315847265 2203 84-85 0.7448587034547245 2203 86-87 0.7219759563244068 2203 88-89 0.8317255507367562 2203 90-91 0.9048425224461845 2203 92-93 0.7274995471462979 2203 94-95 0.7485043957580721 2203 96-97 0.6651131643433246 2203 98-99 0.5945781758186328 2203 100 0.8574211676859029 2204 1 0.691883570295694 2204 2 0.6727790551971395 2204 3 0.6574339562025315 2204 4 0.4650375474414403 2204 5 0.5060263478451361 2204 6 0.5400770156825914 2204 7 0.5591927741653535 2204 8 0.5598182144025046 2204 9 0.7101421889925916 2204 10-11 0.6413490294170927 2204 12-13 0.711516445462415 2204 14-15 0.7988231025645689 2204 16-17 0.8786089378096733 2204 18-19 0.8552624677782106 2204 20-21 0.8221178564447769 2204 22-23 0.8168028982821127 2204 24-25 0.8672832563349075 2204 26-27 0.824589961865783 2204 28-29 0.8734252602928478 2204 30-31 0.9596184352044332 2204 32-33 0.9761797912083381 2204 34-35 1.061152173093383 2204 36-37 1.1477908831090247 2204 38-39 1.2112656486451883 2204 40-41 1.2151253187495357 2204 42-43 1.2176852830442542 2204 44-45 1.1170377782721204 2204 46-47 1.1377538415695128 2204 48-49 1.200042770845755 2204 50-51 1.1902369830096617 2204 52-53 1.1138358248605869 2204 54-55 1.1166953646385593 2204 56-57 1.1780685096541177 2204 58-59 1.0064221534043583 2204 60-61 1.0293379727503051 2204 62-63 1.041577393488332 2204 64-65 1.1371164886191778 2204 66-67 1.0112907147072505 2204 68-69 1.0849955877898765 2204 70-71 1.0656197948079793 2204 72-73 1.0508683755124437 2204 74-75 1.1206646300721914 2204 76-77 1.0875636782975668 2204 78-79 0.9233936475841347 2204 80-81 0.8735312184586768 2204 82-83 0.8364058282265212 2204 84-85 0.8747655517531889 2204 86-87 0.9477505409524412 2204 88-89 0.8663883212891292 2204 90-91 0.9169539788107741 2204 92-93 0.992623684902437 2204 94-95 0.94721440018942 2204 96-97 0.8610819407044978 2204 98-99 0.8835813631844935 2204 100 1.0367155025034727 2205 1 0.6427049937695628 2205 2 0.43380931389737754 2205 3 0.5182119180710245 2205 4 0.35144909300952776 2205 5 0.4127142637574721 2205 6 0.4598707661137098 2205 7 0.46801960582820357 2205 8 0.5179343590136369 2205 9 0.6921028896016566 2205 10-11 0.6682062993879399 2205 12-13 0.6832194935448044 2205 14-15 0.7918416104059247 2205 16-17 0.7936353822312725 2205 18-19 0.7750987340540831 2205 20-21 0.815063317292676 2205 22-23 0.8216360930051181 2205 24-25 0.7128786148194024 2205 26-27 0.7687311374635968 2205 28-29 0.7571396527305794 2205 30-31 0.9042733800791751 2205 32-33 0.9572077462439239 2205 34-35 0.8826742359968804 2205 36-37 0.9198535696852517 2205 38-39 0.9080909623188589 2205 40-41 0.9231358245015429 2205 42-43 0.9281547664290173 2205 44-45 1.001225701776569 2205 46-47 1.003133059043705 2205 48-49 0.931565297952865 2205 50-51 1.0269996205191418 2205 52-53 1.0480769907838692 2205 54-55 1.0313882982882419 2205 56-57 1.049258243986479 2205 58-59 1.091640229319431 2205 60-61 1.0918642468916104 2205 62-63 1.0947341298853246 2205 64-65 1.0442264176741176 2205 66-67 1.0497075222618903 2205 68-69 1.1957650356435323 2205 70-71 1.1657827866740078 2205 72-73 1.1640924084179716 2205 74-75 1.0672462910794778 2205 76-77 0.9065051812673808 2205 78-79 0.8093506966027348 2205 80-81 0.8234581823110965 2205 82-83 0.7540160321160236 2205 84-85 0.8121849939886978 2205 86-87 0.784877816905869 2205 88-89 0.9376883690543778 2205 90-91 0.9420330127293042 2205 92-93 0.8956875588854736 2205 94-95 0.9780553071998881 2205 96-97 0.9571777280350346 2205 98-99 1.0824329581577317 2205 100 1.2090322212520483 2206 1 0.6080252052049318 2206 2 0.591106312801756 2206 3 0.5579237090199811 2206 4 0.23303542314564396 2206 5 0.3847303570595102 2206 6 0.48546476940268946 2206 7 0.5227022019523417 2206 8 0.5619971294678265 2206 9 0.7016764145684888 2206 10-11 0.6507879957862102 2206 12-13 0.7084049568785389 2206 14-15 0.8923750045556602 2206 16-17 0.8244388627619372 2206 18-19 0.8329385699509686 2206 20-21 0.8334203195610641 2206 22-23 0.8305930403869795 2206 24-25 0.8576558375022856 2206 26-27 0.8390397940632326 2206 28-29 0.8457582027736379 2206 30-31 0.8883803695955095 2206 32-33 0.9646530483664151 2206 34-35 1.0961972510291318 2206 36-37 1.0844887956200182 2206 38-39 1.0435066497472647 2206 40-41 1.0161621586508645 2206 42-43 1.050657735233493 2206 44-45 1.0145793147388957 2206 46-47 1.0962934478358974 2206 48-49 1.0500349293068112 2206 50-51 0.9976365423716018 2206 52-53 1.103789605030677 2206 54-55 1.0204718029487552 2206 56-57 1.0338685707467832 2206 58-59 1.12788045819034 2206 60-61 1.08632711565253 2206 62-63 1.1293986370637796 2206 64-65 1.1836458809749715 2206 66-67 1.132855915692872 2206 68-69 1.1778946137887516 2206 70-71 1.1869623663578963 2206 72-73 1.115656948351294 2206 74-75 1.1312957445960308 2206 76-77 1.05201685898653 2206 78-79 0.9380368831149042 2206 80-81 0.8458082107871654 2206 82-83 0.7686169750513194 2206 84-85 0.8647412115737119 2206 86-87 1.011605688510187 2206 88-89 0.9813146188082946 2206 90-91 0.9853778741998518 2206 92-93 1.0282931047850674 2206 94-95 1.0514656109432607 2206 96-97 1.0750655074367366 2206 98-99 1.1115072181065457 2206 100 1.2878504494796559 2207 1 0.7367040536723266 2207 2 0.6512881387089209 2207 3 0.6704921975241334 2207 4 0.41146351229457423 2207 5 0.4873951199819828 2207 6 0.5452830748637254 2207 7 0.5862792800895278 2207 8 0.5819816198094969 2207 9 0.7013487124836573 2207 10-11 0.7565828626968809 2207 12-13 0.7735011337970192 2207 14-15 0.9329889723207714 2207 16-17 0.8869272782417994 2207 18-19 0.8744788888021162 2207 20-21 0.8532237167838161 2207 22-23 0.9304817263621672 2207 24-25 1.014565353342725 2207 26-27 0.9892628736910396 2207 28-29 1.069664064730702 2207 30-31 1.143798384240192 2207 32-33 1.2007510597186837 2207 34-35 1.2262636761288306 2207 36-37 1.156168212870675 2207 38-39 1.228787591936431 2207 40-41 1.286927109532499 2207 42-43 1.358542824183111 2207 44-45 1.344956281952058 2207 46-47 1.4316791869554706 2207 48-49 1.3975854665514404 2207 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0.8404255593892884 2311 30-31 0.8135637657090342 2311 32-33 0.9457813505098898 2311 34-35 0.9617698309753493 2311 36-37 1.041712760654839 2311 38-39 1.1171244640152125 2311 40-41 1.0853656474344007 2311 42-43 1.2346149394207302 2311 44-45 1.2159395559514437 2311 46-47 1.2641496123196205 2311 48-49 1.2085461098468713 2311 50-51 1.2474558184689428 2311 52-53 1.2590496202267047 2311 54-55 1.2299339224826085 2311 56-57 1.2304816502202485 2311 58-59 1.2273599242742605 2311 60-61 1.3085078301325197 2311 62-63 1.3304824308238707 2311 64-65 1.341491781617016 2311 66-67 1.4146052406222296 2311 68-69 1.396639782829027 2311 70-71 1.409660346264637 2311 72-73 1.3828428958495351 2311 74-75 1.2824580753449624 2311 76-77 1.1780016631613286 2311 78-79 1.1242793306225138 2311 80-81 1.2057473089177186 2311 82-83 1.212604196337768 2311 84-85 1.2604897020076926 2311 86-87 1.315598653700384 2311 88-89 1.2685518158264486 2311 90-91 1.3883484500419065 2311 92-93 1.513739984324939 2311 94-95 1.5045629855218543 2311 96-97 1.5120736206517087 2311 98-99 1.5276956432767435 2311 100 1.5850250318614378 2312 1 0.14270611622174556 2312 2 0.13018079332609744 2312 3 0.30523661569261407 2312 4 0.28241256923170965 2312 5 0.32828238987048763 2312 6 0.3918365921385245 2312 7 0.4484394478440805 2312 8 0.23500120369442357 2312 9 0.29462869273660175 2312 10-11 0.2231795686993152 2312 12-13 0.37246181995144667 2312 14-15 0.7222222278199268 2312 16-17 0.831764266681148 2312 18-19 0.8335192070921664 2312 20-21 0.8000788357043547 2312 22-23 0.3421559985721885 2312 24-25 0.3414982680184053 2312 26-27 0.703294062745421 2312 28-29 0.8592934139862223 2312 30-31 0.8170189981278781 2312 32-33 0.5845219875499197 2312 34-35 0.80647534481637 2312 36-37 0.9755273001816249 2312 38-39 0.9754890630481228 2312 40-41 1.0128783258066463 2312 42-43 0.9285837472465133 2312 44-45 0.8482379177298398 2312 46-47 0.9130183117770727 2312 48-49 1.0077027603864295 2312 50-51 1.0411719569298938 2312 52-53 1.0454897895143205 2312 54-55 0.9663767860104571 2312 56-57 0.9235904869765577 2312 58-59 1.0305354118770964 2312 60-61 0.9757192211711256 2312 62-63 0.9639815114084556 2312 64-65 0.958937704604331 2312 66-67 0.9544009744472604 2312 68-69 0.9828516211970424 2312 70-71 0.9061228611150298 2312 72-73 0.9752096238549157 2312 74-75 1.0238982080441374 2312 76-77 0.9713231215635396 2312 78-79 0.9297103772222286 2312 80-81 0.8661940186992005 2312 82-83 0.8602282233345271 2312 84-85 0.6144937658913072 2312 86-87 0.8607197172704808 2312 88-89 0.651396224546211 2312 90-91 0.953068044257968 2312 92-93 1.1815526613571947 2312 94-95 1.2416611624002947 2312 96-97 1.2397703721772828 2312 98-99 1.2787608660333873 2312 100 1.3718841151146002 2313 1 -0.44702873521750774 2313 2 -0.11713532758560063 2313 3 0.16815309238607057 2313 4 0.09371345696314393 2313 5 0.13997242548015976 2313 6 0.2573036038663119 2313 7 0.12080324191824587 2313 8 0.036283177782969744 2313 9 0.27152964908997745 2313 10-11 0.3720605902324152 2313 12-13 0.49929596069792836 2313 14-15 0.5653660369042228 2313 16-17 0.7150954910880216 2313 18-19 0.5253228875918481 2313 20-21 0.7522680681002143 2313 22-23 0.7933823192341194 2313 24-25 0.6526275082805952 2313 26-27 0.839672516050733 2313 28-29 0.7311169863248423 2313 30-31 0.6971127270945772 2313 32-33 0.7839045105583722 2313 34-35 0.7338585073575459 2313 36-37 0.5821122732747526 2313 38-39 0.30416256436562605 2313 40-41 0.5038905475140396 2313 42-43 0.43017234318504194 2313 44-45 0.7321350322552931 2313 46-47 0.7244614825699216 2313 48-49 0.7884348826957606 2313 50-51 0.6946377166408553 2313 52-53 0.6536467773819084 2313 54-55 0.6530044569905087 2313 56-57 0.7702998484424199 2313 58-59 0.6818954563850141 2313 60-61 0.6382410291071423 2313 62-63 0.7144143816857635 2313 64-65 0.7624866665586083 2313 66-67 0.6374742552260173 2313 68-69 0.604404244346469 2313 70-71 0.5847072317283235 2313 72-73 0.621855059838559 2313 74-75 0.25559607663276296 2313 76-77 0.5530616371621555 2313 78-79 0.4590420859102551 2313 80-81 0.4947233750480571 2313 82-83 0.5153200950255901 2313 84-85 0.4937558892696181 2313 86-87 0.6833355820911962 2313 88-89 0.6375617905261777 2313 90-91 0.760634325840222 2313 92-93 0.5362466406902087 2313 94-95 0.5503943318240339 2313 96-97 0.9577885951544793 2313 98-99 1.0908525548684835 2313 100 0.9861553840701589 2314 1 -0.27109960122769294 2314 2 -0.1164169633931138 2314 3 0.17992456831579062 2314 4 -0.17500686693266942 2314 5 0.2147972470331183 2314 6 0.25847557618641304 2314 7 0.1159847904906357 2314 8 0.28008556153181274 2314 9 0.23449067510870236 2314 10-11 0.4157782992535175 2314 12-13 0.4923232338412049 2314 14-15 0.49035372520243925 2314 16-17 0.7451355894654199 2314 18-19 0.8298407555392231 2314 20-21 0.4450523394835315 2314 22-23 0.5913261379245185 2314 24-25 0.8315201669471932 2314 26-27 0.786810917011266 2314 28-29 0.6249816730955331 2314 30-31 0.6417707196407321 2314 32-33 0.7461915707782083 2314 34-35 0.7812205164238932 2314 36-37 0.6796649866067597 2314 38-39 0.6518823391377495 2314 40-41 0.6934993804155596 2314 42-43 0.5181153919401069 2314 44-45 0.5694393433847083 2314 46-47 0.7665500701671348 2314 48-49 0.6099714992948364 2314 50-51 0.7239266740975694 2314 52-53 0.8042926151187615 2314 54-55 0.8508117392713217 2314 56-57 0.8213748888095509 2314 58-59 0.7681085112923043 2314 60-61 0.9287205608121454 2314 62-63 0.6598644633642792 2314 64-65 0.711222138568921 2314 66-67 0.9403817053672654 2314 68-69 0.9392329591573372 2314 70-71 0.9252819521364728 2314 72-73 0.9226114123498164 2314 74-75 1.0934446085621943 2314 76-77 0.8808837588569709 2314 78-79 0.7196827193200761 2314 80-81 0.7174957196972578 2314 82-83 0.7044226591281593 2314 84-85 0.6211277077479238 2314 86-87 0.8803805557572311 2314 88-89 1.0476722944052739 2314 90-91 1.0180165348137464 2314 92-93 1.074854085529065 2314 94-95 1.1535266248730416 2314 96-97 1.1973103018832028 2314 98-99 1.2960507623718662 2314 100 1.3122404299648451 2315 1 -0.1795670646549432 2315 2 0.02737966297723915 2315 3 0.03734976153186409 2315 4 -0.0883346026464622 2315 5 0.2801052634135246 2315 6 0.33271768796203816 2315 7 0.4634128316184061 2315 8 0.40108661885994934 2315 9 0.5493535006953891 2315 10-11 0.5899915227294699 2315 12-13 0.5869790620761179 2315 14-15 0.6998933291790408 2315 16-17 0.7406497193629349 2315 18-19 0.7968225747328361 2315 20-21 0.7459771742555503 2315 22-23 0.8642800606773662 2315 24-25 0.8455747748355762 2315 26-27 0.869593081227741 2315 28-29 0.9953358125515521 2315 30-31 0.908595494508404 2315 32-33 0.9246283782364628 2315 34-35 0.9693254930677426 2315 36-37 1.0314299553288393 2315 38-39 1.0513383716366818 2315 40-41 0.9680916707585112 2315 42-43 1.084243001298276 2315 44-45 0.9272221622935248 2315 46-47 0.8165440142629592 2315 48-49 0.9243441239891794 2315 50-51 0.8465079950703114 2315 52-53 0.9105884640419575 2315 54-55 0.9320017735444637 2315 56-57 0.97525377727289 2315 58-59 0.9365080667561969 2315 60-61 1.0915425466904267 2315 62-63 1.0701478796798725 2315 64-65 1.067755722649423 2315 66-67 1.0197598785792152 2315 68-69 0.903693803644984 2315 70-71 0.47769416607683723 2315 72-73 0.7230178126221674 2315 74-75 0.9010608082695484 2315 76-77 0.8791218180869897 2315 78-79 0.8300225210137668 2315 80-81 0.8086595826439655 2315 82-83 0.7376912597560086 2315 84-85 0.6995696709083852 2315 86-87 0.7665843876715215 2315 88-89 0.9298878223982818 2315 90-91 0.9545052397340861 2315 92-93 0.9645044634502185 2315 94-95 1.0053402863381784 2315 96-97 1.0813827010360306 2315 98-99 0.9919465779057504 2315 100 1.0215422987841372 2316 1 0.5292205789073563 2316 2 0.501663042265271 2316 3 0.49628757062981776 2316 4 0.32054180157808077 2316 5 0.41722158524425623 2316 6 0.4565956936854505 2316 7 0.4587444246058965 2316 8 0.5138816831302648 2316 9 0.45334575586286263 2316 10-11 0.4749404842368463 2316 12-13 0.6619461982163344 2316 14-15 0.7055514989624712 2316 16-17 0.7480135010100994 2316 18-19 0.8131710719167131 2316 20-21 0.8478165364027035 2316 22-23 0.9014771734786606 2316 24-25 0.9859157531134528 2316 26-27 1.0695180451573307 2316 28-29 0.9530503273436466 2316 30-31 0.8313808341538049 2316 32-33 0.7987644872283965 2316 34-35 0.6734831549167453 2316 36-37 0.700695310749957 2316 38-39 0.623571307579482 2316 40-41 0.731015135123009 2316 42-43 0.7633343372560901 2316 44-45 0.7028903288810753 2316 46-47 0.7515485990593689 2316 48-49 0.45488547686035474 2316 50-51 0.5697509337767102 2316 52-53 0.7644077297576146 2316 54-55 0.8051228142154159 2316 56-57 0.8877913030909923 2316 58-59 0.8081450191923523 2316 60-61 0.8633548857122193 2316 62-63 0.7943526071835691 2316 64-65 0.7768340464845025 2316 66-67 0.8677416119747008 2316 68-69 0.911409061148305 2316 70-71 0.7494037263179649 2316 72-73 0.8306543249463303 2316 74-75 0.8853199556032649 2316 76-77 0.7284030737546097 2316 78-79 0.7546202984126325 2316 80-81 0.7269114930351002 2316 82-83 0.6075864431892235 2316 84-85 0.7439451543933764 2316 86-87 0.6096455925021118 2316 88-89 0.6253133794739512 2316 90-91 0.6625883750036792 2316 92-93 0.7942400502494564 2316 94-95 0.7510988048578824 2316 96-97 0.9167775694618463 2316 98-99 0.9276781302264681 2316 100 0.6642276650515129 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 19.0 13 105.0 14 523.0 15 1507.0 16 3464.0 17 6811.0 18 10524.0 19 13916.0 20 18335.0 21 23039.0 22 26676.0 23 26562.0 24 25846.0 25 27372.0 26 31259.0 27 37593.0 28 44883.0 29 54454.0 30 66272.0 31 82122.0 32 102217.0 33 131388.0 34 180247.0 35 249659.0 36 371621.0 37 574662.0 38 536130.0 39 85809.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34544818229675 20.11330946971666 13.052883365780335 23.488358982206254 2 15.9260293249724 21.820551483354183 39.517326746258455 22.73609244541496 3 18.41045228572401 27.235934927876727 31.18400865852503 23.169604127874237 4 12.348423272670315 18.00662902873421 39.183437162743495 30.46151053585198 5 12.247510090814586 37.99173854005096 35.274253173607384 14.486498195527073 6 28.269121282328612 38.318817077615016 19.206572343935004 14.205489296121371 7 25.31894146595805 33.82036388251114 23.389211587892536 17.471483063638278 8 25.15025513919045 35.19204776551051 21.38719905979397 18.270498035505074 9 26.41066349748462 16.04500862975199 21.373464271875424 36.17086360088796 10-11 23.994285415295312 26.4767342344897 29.420707667415414 20.10827268279957 12-13 24.739436689289985 24.985757518877463 28.84487532294849 21.429930468884066 14-15 22.24477758735 26.38611825418547 27.14984991006312 24.219254248401406 16-17 21.093483725825564 28.787938938755257 28.06582738952586 22.052749945893314 18-19 20.635950075904795 27.99897110495351 30.39751865055219 20.967560168589504 20-21 21.645940262218364 27.496540009202246 30.1193639654193 20.738155763160083 22-23 21.564827888763713 27.3483925710265 29.81805169282873 21.268727847381054 24-25 21.119074198681535 27.33059351407798 30.22860732806518 21.321724959175302 26-27 21.08901209373122 27.50943779380193 30.010363964504567 21.391186147962287 28-29 20.821272424508862 28.017184759767915 30.033995571903873 21.127547243819343 30-31 21.668854127751874 27.55416115837499 29.983340657394564 20.793644056478573 32-33 21.38697535751357 27.744612069162685 29.668452900353266 21.19995967297048 34-35 21.531780510437354 27.61142451850858 30.02232635045508 20.83446862059898 36-37 21.46066870836128 27.330299766763687 29.90276177779323 21.306269747081803 38-39 21.186294446059783 27.446698214280158 30.06987965343615 21.297127686223906 40-41 21.16117144211477 26.8765062296368 30.58834635401183 21.3739759742366 42-43 20.89668153079956 27.181824069503698 29.984024746640753 21.93746965305599 44-45 20.53126334498086 27.566581734497138 30.21773885754705 21.684416062974954 46-47 20.508673612778193 27.061492555228323 29.945957339672464 22.483876492321023 48-49 20.53218679961579 27.09514705209715 30.01745414627453 22.355212002012532 50-51 20.471916394870956 27.30589065743641 29.695387541711632 22.52680540598101 52-53 20.194807606766247 27.586283872811673 29.452679295328778 22.766229225093305 54-55 20.036197949209797 27.330732413832727 29.58494396581614 23.04812567114134 56-57 20.398866643236495 27.12139859459224 29.16132367902959 23.318411083141672 58-59 19.98768296478283 27.210566596427366 29.27943331392101 23.522317124868795 60-61 20.383489255699995 27.398221138506486 28.81401793790231 23.404271667891212 62-63 20.352549472531443 27.127981583651557 28.696053229291625 23.823415714525378 64-65 19.932947997186464 26.935329693695092 28.67303859534069 24.458683713777756 66-67 20.17250767590521 26.994952892134656 28.190646148777574 24.64189328318256 68-69 20.043803329821866 27.202362048341083 28.027156966202977 24.72667765563407 70-71 20.192372283382127 27.01576775485438 27.89729406693494 24.894565894828556 72-73 20.356787401620917 27.26408695464377 27.451154083942097 24.927971559793214 74-75 20.56198025875387 27.46087563761575 27.09657867932242 24.88056542430796 76-77 20.538446222721934 27.33898512278606 27.194213375730904 24.9283552787611 78-79 20.559173781901915 27.350918553770686 27.02642174339045 25.06348592093695 80-81 20.521281753400597 27.454349198218058 26.795762859155314 25.228606189226028 82-83 20.80937041302263 27.113328730305447 26.966124557348962 25.111176299322956 84-85 21.095438640548064 27.179886946623526 26.526415514629342 25.198258898199065 86-87 20.75826583672578 27.25333361643135 26.799803271283007 25.18859727555986 88-89 20.878040636645913 26.744422318852358 26.781985789585256 25.595551254916476 90-91 21.11550299609196 26.43006611497043 26.74169920927769 25.712731679659917 92-93 21.10121341616507 26.42547302881177 26.74061207355306 25.732701481470095 94-95 20.667470348159725 26.27767701774033 27.088243898610255 25.96660873548969 96-97 20.891265016651577 26.147191406129224 27.074970420880636 25.886573156338567 98-99 20.871190135138992 26.291992595645077 26.86230578756177 25.974511481654165 100 20.85601831266571 26.182171500090572 27.028272281965283 25.93353790527843 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2529.0 1 1653.0 2 1589.0 3 2360.5 4 1685.5 5 1108.0 6 1486.5 7 2283.0 8 3196.0 9 3609.0 10 3096.0 11 2463.0 12 2475.0 13 2753.0 14 2982.5 15 3267.5 16 3573.0 17 3915.5 18 4303.5 19 4756.0 20 5297.5 21 5978.0 22 6833.5 23 7969.5 24 9390.0 25 11122.5 26 13789.0 27 17049.5 28 20449.0 29 24959.0 30 30811.0 31 36406.0 32 42968.5 33 49979.0 34 56734.5 35 63004.0 36 69618.5 37 77451.5 38 81334.0 39 82174.5 40 83120.5 41 84316.5 42 86635.0 43 90989.5 44 96275.0 45 102794.0 46 108502.5 47 111293.0 48 114232.5 49 115698.5 50 114808.0 51 112926.5 52 109317.0 53 103791.5 54 95860.5 55 85095.5 56 74156.5 57 62402.0 58 50591.5 59 41500.5 60 32288.0 61 23868.0 62 18026.0 63 13454.5 64 9633.0 65 6573.0 66 4586.5 67 3491.0 68 2576.5 69 1804.5 70 1386.5 71 1063.0 72 815.5 73 639.5 74 451.0 75 322.5 76 233.0 77 131.0 78 77.0 79 56.0 80 36.0 81 20.0 82 15.5 83 9.5 84 5.0 85 7.5 86 5.5 87 5.0 88 3.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05104226848672297 2 0.0 3 0.0 4 0.018367898767265183 5 0.003585765097992008 6 3.658943977542866E-5 7 1.4635775910171463E-4 8 4.024838375297152E-4 9 0.0015367564705680034 10-11 8.049676750594304E-4 12-13 2.9271551820342926E-4 14-15 3.658943977542866E-5 16-17 9.147359943857164E-5 18-19 0.0 20-21 5.4884159663142975E-5 22-23 0.0 24-25 7.317887955085731E-5 26-27 5.4884159663142975E-5 28-29 9.51325434161145E-4 30-31 0.0010245043137120024 32-33 0.013556387436796316 34-35 0.022228084663572907 36-37 0.03005822477551464 38-39 0.01995953939749633 40-41 1.6465247898942895E-4 42-43 0.00190265086832229 44-45 0.00951325434161145 46-47 0.013647861036234887 48-49 0.005634773725416013 50-51 0.010171864257569167 52-53 0.01593470102219918 54-55 0.010153569537681451 56-57 0.00312839710079915 58-59 0.008360686988685447 60-61 0.0016465247898942896 62-63 8.232623949471448E-4 64-65 0.019136277002549187 66-67 0.046340525475580395 68-69 0.05493904382280612 70-71 0.049267680657614686 72-73 0.04105335142803095 74-75 0.02853976302483435 76-77 0.029161783501016635 78-79 0.012312346484431741 80-81 0.0019758297478731472 82-83 0.028155573907192352 84-85 0.04795046082569925 86-87 0.049340859537165535 88-89 0.06044575450900813 90-91 0.035491756582165794 92-93 0.02718595375314349 94-95 0.006897109397668301 96-97 0.003823596456532294 98-99 0.021331643389074905 100 0.018075183249061755 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2733029.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.65320674043096 #Duplication Level Percentage of deduplicated Percentage of total 1 83.44484234643853 60.62535382418466 2 11.504607632866461 16.716932736363738 3 2.5800865343982666 5.623546811755177 4 0.9364855868040909 2.721547237900456 5 0.4559436430304632 1.6562883879538743 6 0.2771248245773972 1.2080404303756378 7 0.1615969654050896 0.821837641534157 8 0.1212747190491778 0.7048797788374076 9 0.09231629440644905 0.6036367340720008 >10 0.38995352696664176 4.833286695297375 >50 0.02413874560627474 1.1956709506238392 >100 0.010667936454436889 1.3514936704284413 >500 5.340244426403547E-4 0.2460606465892493 >1k 2.67012221312823E-4 0.5685157134004871 >5k 5.340244426256461E-5 0.2347702574071076 >10k+ 1.0680488852512922E-4 0.8881384832764997 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12292 0.449757393719569 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10599 0.3878114721797683 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6051 0.2214027000811188 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4437 0.16234734428357694 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3272 0.11972064694520256 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2862 0.1047189766372768 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.658943977542866E-5 0.0 12-13 0.0 0.0 0.0 1.280630392140003E-4 0.0 14-15 0.0 0.0 0.0 3.110102380911436E-4 0.0 16-17 0.0 0.0 0.0 4.3907327730514386E-4 0.0 18-19 0.0 0.0 0.0 6.403151960700014E-4 0.0 20-21 0.0 0.0 0.0 0.0016282300700065752 0.0 22-23 0.0 0.0 0.0 0.006000668123170299 1.829471988771433E-5 24-25 0.0 0.0 0.0 0.016172532380739464 3.658943977542866E-5 26-27 0.0 0.0 0.0 0.03249142252058065 3.658943977542866E-5 28-29 0.0 0.0 0.0 0.07972838927065903 3.658943977542866E-5 30-31 0.0 0.0 0.0 0.20667545057150874 3.658943977542866E-5 32-33 0.0 0.0 0.0 0.4672654406521116 3.658943977542866E-5 34-35 0.0 0.0 0.0 0.8471370044006119 3.658943977542866E-5 36-37 0.0 0.0 0.0 1.4011925962000404 3.658943977542866E-5 38-39 0.0 0.0 0.0 2.1384149235152643 3.658943977542866E-5 40-41 0.0 0.0 0.0 2.913562205157721 3.658943977542866E-5 42-43 0.0 0.0 0.0 3.6442716121929184 3.658943977542866E-5 44-45 0.0 0.0 0.0 4.491079311635552 3.658943977542866E-5 46-47 0.0 0.0 0.0 5.448734718877846 9.147359943857164E-5 48-49 0.0 0.0 0.0 6.402694592702822 1.4635775910171463E-4 50-51 0.0 0.0 0.0 7.32813299822285 1.4635775910171463E-4 52-53 0.0 0.0 0.0 8.204523259723917 1.8294719887714328E-4 54-55 0.0 0.0 0.0 9.15003097296077 2.1953663865257193E-4 56-57 0.0 0.0 0.0 10.19895873772287 2.1953663865257193E-4 58-59 0.0 0.0 0.0 11.291702356616048 2.1953663865257193E-4 60-61 0.0 0.0 0.0 12.356144043842932 2.1953663865257193E-4 62-63 0.0 0.0 0.0 13.400754254711531 2.1953663865257193E-4 64-65 0.0 0.0 0.0 14.437113546910773 2.1953663865257193E-4 66-67 0.0 0.0 0.0 15.541474312932646 2.1953663865257193E-4 68-69 0.0 0.0 0.0 16.725051216068323 2.1953663865257193E-4 70-71 0.0 0.0 0.0 17.910677127831427 2.1953663865257193E-4 72-73 0.0 0.0 0.0 19.009256762368786 2.561260784280006E-4 74-75 0.0 0.0 0.0 20.07481442750882 2.561260784280006E-4 76-77 0.0 0.0 0.0 21.193353601443672 2.561260784280006E-4 78-79 0.0 0.0 0.0 22.356165997506793 2.561260784280006E-4 80-81 0.0 0.0 0.0 23.498652959774667 2.744207983157149E-4 82-83 0.0 0.0 0.0 24.61408203132861 2.9271551820342926E-4 84-85 0.0 0.0 0.0 25.67460864849952 2.9271551820342926E-4 86-87 0.0 0.0 0.0 26.7512712086114 2.9271551820342926E-4 88 0.0 0.0 0.0 27.56022713260635 2.9271551820342926E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5575 0.0 55.415035 1 TACCTGG 2445 0.0 43.631454 2 GTATCAA 9860 0.0 39.582962 1 ACCTGGG 2650 0.0 39.015526 3 ATCAACG 11740 0.0 32.745064 3 CAACGCA 11920 0.0 32.24469 5 TATCAAC 12100 0.0 32.04212 2 TCAACGC 12290 0.0 31.319622 4 AACGCAG 12745 0.0 30.672472 6 ACGCAGA 14105 0.0 27.248684 7 GTACATG 8890 0.0 27.134556 1 GTGGTAT 2295 0.0 26.840815 1 CGCAGAG 14335 0.0 26.713156 8 TACATGG 8840 0.0 26.527786 2 CCTGGGG 2515 0.0 25.788471 4 ACATGGG 8785 0.0 25.731432 3 GTACCTG 4455 0.0 25.120968 1 GCAGAGT 15305 0.0 24.805233 9 CATGGGG 5805 0.0 24.774431 4 TGGTATC 2460 0.0 23.879631 2 >>END_MODULE