FastQCFastQC Report
Mon 6 Jun 2016
ERR1211446_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211446_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3361152
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184780.5497519897939754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152280.45305895121672574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85510.2544068224227884No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69090.20555452416314407No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67610.20115127194485702No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49570.14747919760843903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69200.053.3102761
TACCTGG32100.045.745142
GTATCAA122600.040.1204031
ACCTGGG36550.038.971413
ATCAACG151250.032.137793
TATCAAC151650.032.0282142
CAACGCA151850.032.0103265
TCAACGC157400.030.9712244
AACGCAG163050.030.3597686
GTGGTAT27350.029.4573731
GTACCTG56200.027.4974731
GTACATG123450.027.4405651
ACGCAGA181450.026.9443197
CCTGGGG35500.026.8814134
TACATGG124700.026.495132
CGCAGAG184150.026.4714958
ACATGGG124500.025.4873833
CATGGGG82750.024.5982554
GCAGAGT195550.024.2548359
TGGTATC33100.023.887832
Produced by FastQC (version 0.11.5)