FastQCFastQC Report
Mon 6 Jun 2016
ERR1211446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211446_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3361152
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165660.49286673140637494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146130.43476165314749227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83740.24914077078335045No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72110.21453953882478388No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53490.1591418656460642No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45420.13513224037472865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68500.056.2879561
TACCTGG33300.045.442082
GTATCAA121950.040.7939761
ACCTGGG37250.040.246113
ATCAACG151050.032.3261763
TATCAAC154850.031.835442
CAACGCA154150.031.702335
TCAACGC159450.030.5941774
AACGCAG163900.030.2155636
CCTGGGG35650.029.7930414
GTGGTAT27950.028.2630921
ACGCAGA182500.026.8528547
GTACCTG57250.026.5288981
CGCAGAG185300.026.294958
CTGGGGG24900.026.2311155
GTACATG122450.025.3441281
TGGGGGG65900.024.8120616
TGGTATC30450.024.5389752
TACATGG123850.024.4742982
TATCACG2352.0190782E-1023.9971662
Produced by FastQC (version 0.11.5)