FastQCFastQC Report
Mon 6 Jun 2016
ERR1211445_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211445_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3538654
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT179650.5076789084211115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153100.4326503806249495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128000.3617194560417605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70670.1997087028005564No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT46510.1314341554726741No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45180.12767566424974017No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43290.12233465040662353No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36900.10427693693703878No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35530.1004054083840918No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35440.10015107439156244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG27300.038.4511572
ACCTGGG34300.029.875923
GTACATG113850.026.5720141
TACATGG114000.025.8898722
CCGTATC12300.025.60015994
GTACTAG11200.024.8231241
ACATGGG115200.024.2377823
CATGGGG86400.023.5573794
ATGGGGG64650.022.2490675
CCTGGGG34350.022.0318974
CGCCGTT2154.9560185E-821.85921194
GAGTACT120600.021.16242212-13
TGGGGGG68600.020.8312116
TCGCCGT24850.020.61451394
GTACCTG51050.020.491781
GAGTACA87800.019.2136961
AGTACAT86350.018.6981662
GTATAAG14150.018.6489351
GTACTTT135800.018.6349514-15
AGAGTAC166900.018.55990410-11
Produced by FastQC (version 0.11.5)