FastQCFastQC Report
Mon 6 Jun 2016
ERR1211445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211445_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3538654
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT183000.5171457848097045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139770.39498069039810046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126320.3569718881812124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73170.20677353592637202No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48370.13669039131828092No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT45430.12838214756232172No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC39260.11094613940780874No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36180.10224226499680386No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG29150.046.266732
ACCTGGG35650.036.776483
CCTGGGG34500.027.6512494
GTACATG106600.026.3752541
TACATGG107200.025.7317222
GTACCTG55250.024.593381
ACATGGG109550.023.9786623
CTGGGGG24900.023.0213495
GTACTAG11250.022.9859681
TGGGGGG59700.022.7447666
CATGGGG81100.022.6565464
ATGGGGG57800.022.3550245
TAGTACT14350.021.2862534
GAGTACT127200.020.4260212-13
CTAGTAC13550.020.1147293
ATAACGC3103.0013325E-1019.7063943
AGAGTAC171700.019.6474410-11
GAGTACA81700.019.5663381
CCGTCGA5050.019.5382619
AGTACTT134800.018.54246312-13
Produced by FastQC (version 0.11.5)