FastQCFastQC Report
Mon 6 Jun 2016
ERR1211444_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211444_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074251
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100190.48301772543438576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82720.3987945528289488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47140.22726275653235795No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28330.1365794207162007No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27990.13494027482691343No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23480.11319748670725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37800.057.197251
GTATCAA69050.042.089691
TACCTGG14450.038.427162
CAACGCA83450.034.641865
ATCAACG83600.034.6359333
TATCAAC84550.034.0613562
TCAACGC86800.033.304884
AACGCAG88600.032.8420526
ACCTGGG17400.031.3372733
GTGGTAT14350.031.1835771
ACGCAGA98050.029.293927
CGCAGAG99800.028.7781648
TAACGCA1755.5515557E-926.860524
ATAACGC1755.5515557E-926.860523
GCAGAGT108750.025.8478479
GTACATG62950.025.5908451
CGTTAAC2051.0550139E-925.2751121
TACATGG64250.024.1693462
TGGTATC17550.024.1317862
ATGGGGG36750.023.2791165
Produced by FastQC (version 0.11.5)