##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211444_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2074251 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.421572413367524 31.0 31.0 34.0 30.0 34.0 2 31.473240220204787 31.0 31.0 34.0 28.0 34.0 3 31.541117251480173 31.0 31.0 34.0 28.0 34.0 4 35.17007584906552 37.0 35.0 37.0 32.0 37.0 5 35.22671364265945 37.0 35.0 37.0 32.0 37.0 6 35.29288692641344 37.0 35.0 37.0 32.0 37.0 7 35.23182054630804 37.0 35.0 37.0 32.0 37.0 8 35.24063722278548 37.0 35.0 37.0 32.0 37.0 9 36.821768435931816 39.0 37.0 39.0 32.0 39.0 10-11 36.75450945907704 39.0 37.0 39.0 32.0 39.0 12-13 36.669707523342154 39.0 37.0 39.0 32.0 39.0 14-15 37.86129668010284 40.0 37.5 41.0 32.0 41.0 16-17 37.85030403745738 40.0 37.5 41.0 32.0 41.0 18-19 37.857360560510756 40.0 37.5 41.0 32.0 41.0 20-21 37.84461909383194 40.0 37.5 41.0 32.0 41.0 22-23 37.71634339335018 40.0 37.0 41.0 32.0 41.0 24-25 37.623955345809165 40.0 37.0 41.0 32.0 41.0 26-27 37.4777189452964 40.0 37.0 41.0 32.0 41.0 28-29 37.340859905575556 40.0 36.0 41.0 31.0 41.0 30-31 37.16725627708507 39.5 36.0 41.0 31.0 41.0 32-33 36.94011742069788 39.0 35.5 41.0 30.5 41.0 34-35 36.79403023067122 39.0 35.0 41.0 30.0 41.0 36-37 36.64532185352689 39.0 35.0 41.0 30.0 41.0 38-39 36.48036303224634 39.0 35.0 41.0 30.0 41.0 40-41 36.30246893939065 39.0 35.0 40.0 29.5 41.0 42-43 36.091720336642 39.0 35.0 40.0 28.5 41.0 44-45 35.94105125175304 38.5 35.0 40.0 28.0 41.0 46-47 35.686258798959244 38.0 35.0 40.0 27.0 41.0 48-49 35.417438873116126 38.0 34.0 40.0 26.0 41.0 50-51 34.741512719531045 37.5 33.5 39.5 25.0 40.5 52-53 35.031636479866705 38.0 34.0 39.5 26.0 40.5 54-55 35.43097291504259 38.0 34.0 40.0 26.0 41.0 56-57 35.349462046782186 38.0 34.0 40.0 26.0 41.0 58-59 35.147782018666014 38.0 34.0 40.0 26.0 41.0 60-61 34.918117190253255 38.0 34.0 40.0 26.0 41.0 62-63 34.63288869090577 37.0 34.0 40.0 25.0 41.0 64-65 34.41493700617717 37.0 33.0 40.0 25.0 41.0 66-67 34.200931806227885 36.5 33.0 39.5 24.5 41.0 68-69 33.85098452405229 36.0 33.0 39.0 23.5 41.0 70-71 33.469081610663316 35.0 33.0 39.0 23.0 41.0 72-73 33.074452898901825 35.0 32.0 38.5 22.5 40.0 74-75 32.69033183544325 35.0 32.0 37.0 21.5 40.0 76-77 32.301823887273045 35.0 31.5 37.0 20.0 39.0 78-79 31.902532287558255 35.0 31.0 36.0 20.0 39.0 80-81 31.507800406026078 35.0 31.0 36.0 19.0 38.5 82-83 31.137637634018255 35.0 30.5 35.5 18.0 37.0 84-85 30.80100238592147 34.0 30.0 35.0 17.5 37.0 86-87 30.463012673008233 34.0 29.5 35.0 16.0 36.0 88-89 30.143889288229822 34.0 29.0 35.0 13.5 36.0 90-91 29.83039685168285 34.0 29.0 35.0 11.0 36.0 92-93 29.53519535485339 34.0 29.0 35.0 10.0 35.0 94-95 29.240221168990637 34.0 27.5 35.0 10.0 35.0 96-97 28.93333690088615 34.0 27.0 35.0 10.0 35.0 98-99 28.61047722768363 34.0 25.5 35.0 9.0 35.0 100 27.351557743011814 32.0 24.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1955302646101984 1101 2 -0.21912553617033126 1101 3 -0.2713669069632232 1101 4 -0.26740487159410264 1101 5 -0.1743185437680097 1101 6 -0.3194983773540727 1101 7 -0.279832515828943 1101 8 -0.4638206462870684 1101 9 -0.3721859699363108 1101 10-11 -0.35745686748284555 1101 12-13 -0.37269275943998537 1101 14-15 -0.5564500487563677 1101 16-17 -0.5081009129041831 1101 18-19 -0.49073801546583695 1101 20-21 -0.5710111322180538 1101 22-23 -0.44847524061956534 1101 24-25 -0.6118119408780345 1101 26-27 -0.5646193016827752 1101 28-29 -0.7383163620869553 1101 30-31 -0.8645353413460626 1101 32-33 -0.5757909825367236 1101 34-35 -0.5342919742087275 1101 36-37 -0.6660109660002078 1101 38-39 -0.5352051842234715 1101 40-41 -0.7008564025670623 1101 42-43 -0.864642994262887 1101 44-45 -0.793427726321525 1101 46-47 -0.7939592476988437 1101 48-49 -0.975831080212096 1101 50-51 -0.746783399183407 1101 52-53 -0.45590433018171694 1101 54-55 -0.5002196340271112 1101 56-57 -0.5242693097939295 1101 58-59 -0.6568443400623494 1101 60-61 -0.8346648612204817 1101 62-63 -0.8761245089786414 1101 64-65 -0.784064415116255 1101 66-67 -0.6927112346753646 1101 68-69 -0.8272333352163628 1101 70-71 -0.9154145119669224 1101 72-73 -1.163391413707835 1101 74-75 -1.022013903112768 1101 76-77 -1.071505814163558 1101 78-79 -0.9387980452182276 1101 80-81 -1.2930609596094058 1101 82-83 -0.9828640699228615 1101 84-85 -1.1086768064378916 1101 86-87 -1.149308666354191 1101 88-89 -1.2158695719582298 1101 90-91 -1.360753893469493 1101 92-93 -1.4150022474524313 1101 94-95 -1.535205758580524 1101 96-97 -1.5237943694583258 1101 98-99 -1.8361440194860101 1101 100 -1.625440040170492 1102 1 -0.43720224616345504 1102 2 -0.3739211992228739 1102 3 -0.47694508485656684 1102 4 -0.394976867865509 1102 5 -0.32783372799015353 1102 6 -0.3011939079432864 1102 7 -0.3645599847981842 1102 8 -0.3671224173708154 1102 9 -0.5511243008983868 1102 10-11 -0.43640255751866164 1102 12-13 -0.5226314338255023 1102 14-15 -0.7744650612667883 1102 16-17 -0.7998047837769704 1102 18-19 -0.7799250831202542 1102 20-21 -0.9336834571947463 1102 22-23 -0.8806172216822219 1102 24-25 -0.8274401604727899 1102 26-27 -0.8776742984938508 1102 28-29 -0.9255238389058817 1102 30-31 -0.8290278105606035 1102 32-33 -0.7962639256756106 1102 34-35 -0.8432528729969349 1102 36-37 -0.9460163626542837 1102 38-39 -1.0143495692502142 1102 40-41 -1.1961760316697436 1102 42-43 -0.8916409827827323 1102 44-45 -0.8779614063490015 1102 46-47 -1.1255664694032035 1102 48-49 -1.1125724823609389 1102 50-51 -0.6949558958718214 1102 52-53 -0.6503138871654741 1102 54-55 -0.7187743119449834 1102 56-57 -0.8881117274949588 1102 58-59 -0.7751242792273416 1102 60-61 -0.785236415947459 1102 62-63 -0.8025484897469326 1102 64-65 -0.7448773474618449 1102 66-67 -0.8136772357547031 1102 68-69 -0.8336725247370396 1102 70-71 -0.9247924249336066 1102 72-73 -0.9529685122703668 1102 74-75 -1.0836706759136305 1102 76-77 -0.9625596335228295 1102 78-79 -0.9836000861346754 1102 80-81 -1.0068813000892156 1102 82-83 -1.0924872853474774 1102 84-85 -1.2696639035286026 1102 86-87 -1.188152310567503 1102 88-89 -1.2581400915088388 1102 90-91 -1.2686428205165399 1102 92-93 -1.618014561635821 1102 94-95 -1.6685104732937681 1102 96-97 -1.7138767910841857 1102 98-99 -1.6457034562715656 1102 100 -1.5523104714122127 1103 1 -0.5099225777200402 1103 2 -0.5502147876056682 1103 3 -0.620308313426424 1103 4 -0.5478668532022866 1103 5 -0.569117208293136 1103 6 -0.561947950452172 1103 7 -0.4601283365304383 1103 8 -0.5639479886436263 1103 9 -0.9121771029622323 1103 10-11 -0.7829169329723698 1103 12-13 -0.8451566895642557 1103 14-15 -0.8253154992835832 1103 16-17 -0.9234305042849442 1103 18-19 -0.8960248393116359 1103 20-21 -0.8929406651666838 1103 22-23 -0.8688480329604644 1103 24-25 -0.9445864427528505 1103 26-27 -1.0349959320983686 1103 28-29 -0.9930387262709175 1103 30-31 -0.9203237271277374 1103 32-33 -1.0559825768904645 1103 34-35 -1.0144711641385555 1103 36-37 -1.0323953274365252 1103 38-39 -1.1810073666432288 1103 40-41 -1.0310033555881475 1103 42-43 -1.2366900481830143 1103 44-45 -1.1697102343034018 1103 46-47 -1.339821175661534 1103 48-49 -1.3123142129683245 1103 50-51 -1.1829387049057516 1103 52-53 -1.157963201869478 1103 54-55 -1.0138267033228985 1103 56-57 -1.0756948407835338 1103 58-59 -0.9520998376300653 1103 60-61 -1.1551632155182574 1103 62-63 -1.0596370157025703 1103 64-65 -0.9548759592276994 1103 66-67 -0.94219980757412 1103 68-69 -0.970135217390677 1103 70-71 -1.1328882882850948 1103 72-73 -1.069126256158519 1103 74-75 -1.2124922965034557 1103 76-77 -1.295932926790183 1103 78-79 -1.2346457445770156 1103 80-81 -1.1833076383613843 1103 82-83 -1.1336297731208944 1103 84-85 -1.3491507196425303 1103 86-87 -1.4684779960804129 1103 88-89 -1.5758993659251281 1103 90-91 -1.404306555502643 1103 92-93 -1.455547785260098 1103 94-95 -1.6112367048454352 1103 96-97 -1.5693415169224068 1103 98-99 -1.5042474589554864 1103 100 -1.6414426226256147 1104 1 -0.4225711406679835 1104 2 -0.4497787569967322 1104 3 -0.5150209016015523 1104 4 -0.36797305774002353 1104 5 -0.4426191393746848 1104 6 -0.4139370026149791 1104 7 -0.4724594958833279 1104 8 -0.5118960042279781 1104 9 -0.7043061610111536 1104 10-11 -0.614574004779314 1104 12-13 -0.7517899356047693 1104 14-15 -0.9545246591210415 1104 16-17 -0.896409215598645 1104 18-19 -0.9355210476858105 1104 20-21 -0.8382795084175925 1104 22-23 -0.9186585152799225 1104 24-25 -0.8671695940415916 1104 26-27 -0.9227500677170895 1104 28-29 -1.0489672922269548 1104 30-31 -0.9741585093258749 1104 32-33 -1.099254720434807 1104 34-35 -0.8752382195282706 1104 36-37 -1.0901247849045248 1104 38-39 -1.0052399310024782 1104 40-41 -0.8696354187510948 1104 42-43 -0.882821227156299 1104 44-45 -0.9713182862392031 1104 46-47 -0.9366771922647956 1104 48-49 -1.1282828904608877 1104 50-51 -0.7453367686505246 1104 52-53 -0.828824975105853 1104 54-55 -0.6808824322923996 1104 56-57 -0.7170099778562644 1104 58-59 -0.8657250378979597 1104 60-61 -0.755981193447866 1104 62-63 -0.8969193997323686 1104 64-65 -1.0117391793970043 1104 66-67 -0.935489295654456 1104 68-69 -0.9441263294115032 1104 70-71 -0.7378739023565899 1104 72-73 -0.8267182012824961 1104 74-75 -0.9240430506868549 1104 76-77 -0.9559877960248215 1104 78-79 -0.7676867322458563 1104 80-81 -0.942568730029592 1104 82-83 -0.8148103437349263 1104 84-85 -0.8804781745870223 1104 86-87 -0.979334092409168 1104 88-89 -1.0956780739593377 1104 90-91 -1.206058085058892 1104 92-93 -1.2409141705598437 1104 94-95 -1.131537021174715 1104 96-97 -1.1966902738407832 1104 98-99 -1.483896819441096 1104 100 -1.384022567931666 1105 1 -0.609929518890727 1105 2 -0.6471832313084356 1105 3 -0.5662498976907919 1105 4 -0.5858859587745968 1105 5 -0.4663364017869469 1105 6 -0.5060067421678838 1105 7 -0.6688380596644947 1105 8 -0.537175893306646 1105 9 -0.5762579907379575 1105 10-11 -0.7061886537753423 1105 12-13 -0.8196902573549139 1105 14-15 -0.9485292323897809 1105 16-17 -0.929751307841137 1105 18-19 -0.8582673291095588 1105 20-21 -0.9195123342000926 1105 22-23 -0.8140396815752666 1105 24-25 -0.8638037968364287 1105 26-27 -1.0466582557130977 1105 28-29 -0.9605505768737927 1105 30-31 -0.7482791474590016 1105 32-33 -0.8550874550873786 1105 34-35 -0.9512742633712676 1105 36-37 -1.0278345444724692 1105 38-39 -0.9568629382341669 1105 40-41 -0.9234487589424702 1105 42-43 -0.9616944587185614 1105 44-45 -0.9683357378997997 1105 46-47 -1.0528391966759258 1105 48-49 -1.1029468139117427 1105 50-51 -1.0174157889199478 1105 52-53 -0.9243780582884753 1105 54-55 -0.8848170926936447 1105 56-57 -0.8447962194735652 1105 58-59 -0.7454451348423135 1105 60-61 -0.7119743838619002 1105 62-63 -0.8209976876078926 1105 64-65 -0.7788345667131793 1105 66-67 -0.6484365940434671 1105 68-69 -0.6807313482664483 1105 70-71 -0.736289751136816 1105 72-73 -0.7433428438211678 1105 74-75 -0.6039915315286066 1105 76-77 -0.6069446602607975 1105 78-79 -0.6274503535143552 1105 80-81 -0.692442797415417 1105 82-83 -0.7125407612833072 1105 84-85 -0.6862414436355522 1105 86-87 -0.7360132731343505 1105 88-89 -0.846910144984772 1105 90-91 -0.8392310558106573 1105 92-93 -0.8853942407802045 1105 94-95 -0.7671735748980417 1105 96-97 -1.0561227803889999 1105 98-99 -0.9173612102822624 1105 100 -1.3079254446742432 1106 1 -0.6784446844415584 1106 2 -0.6120954381381978 1106 3 -0.5944616118682298 1106 4 -0.43881450313435977 1106 5 -0.5681371793635464 1106 6 -0.5784168085566463 1106 7 -0.5697166931508164 1106 8 -0.6544973243844439 1106 9 -0.7780826289683418 1106 10-11 -0.7964156106998459 1106 12-13 -0.7742069387590433 1106 14-15 -1.0282511855948329 1106 16-17 -0.8365642262798119 1106 18-19 -1.0159981809349006 1106 20-21 -0.9735202222515227 1106 22-23 -0.9857451568935289 1106 24-25 -0.9698693153845781 1106 26-27 -1.0179300285596682 1106 28-29 -1.0633759589951453 1106 30-31 -0.977141415343965 1106 32-33 -1.0044705870087185 1106 34-35 -1.043180589251648 1106 36-37 -0.9603517030896711 1106 38-39 -0.9852592215864533 1106 40-41 -0.9193643764421537 1106 42-43 -0.9686351018333568 1106 44-45 -0.9090693828252014 1106 46-47 -0.8877213671532829 1106 48-49 -0.9311880030144479 1106 50-51 -0.8647019904442246 1106 52-53 -0.6706607087860377 1106 54-55 -0.6350893848603292 1106 56-57 -0.7302454313702071 1106 58-59 -0.6009092245583716 1106 60-61 -0.4502454689602189 1106 62-63 -0.6398567272867552 1106 64-65 -0.6839084645615046 1106 66-67 -0.6202929529076968 1106 68-69 -0.6112582476446704 1106 70-71 -0.5663766319879429 1106 72-73 -0.6701376562343242 1106 74-75 -0.7755419125972836 1106 76-77 -0.7961491179923499 1106 78-79 -0.8339208013928641 1106 80-81 -0.7653132340653812 1106 82-83 -0.912283908363186 1106 84-85 -1.0313689577765004 1106 86-87 -0.9220786513257089 1106 88-89 -0.9326484918625333 1106 90-91 -0.8865411179887133 1106 92-93 -1.0467119102961142 1106 94-95 -0.9388509788589126 1106 96-97 -1.1133918583852669 1106 98-99 -1.2643842428309995 1106 100 -1.2340205785044418 1107 1 -0.5115084383925854 1107 2 -0.45751197316095116 1107 3 -0.6206744113495866 1107 4 -0.3760685683668257 1107 5 -0.31464420470346255 1107 6 -0.41810556814955646 1107 7 -0.4840768900490744 1107 8 -0.6046570067430679 1107 9 -0.5950523159909196 1107 10-11 -0.5702508185057553 1107 12-13 -0.7096308458410121 1107 14-15 -0.82211883593218 1107 16-17 -0.7678284592095252 1107 18-19 -0.8388656520997557 1107 20-21 -0.9104654542799011 1107 22-23 -0.7814906991228483 1107 24-25 -0.9239792725883333 1107 26-27 -0.8285664465321787 1107 28-29 -0.778599192880904 1107 30-31 -0.962492627630553 1107 32-33 -0.9208012710510189 1107 34-35 -0.8160549674478688 1107 36-37 -0.9678154001431736 1107 38-39 -0.9092511027472199 1107 40-41 -0.9058911517885591 1107 42-43 -0.816780803266326 1107 44-45 -0.6971198082573054 1107 46-47 -0.937169967214281 1107 48-49 -1.079611353430714 1107 50-51 -1.0761507026589996 1107 52-53 -1.0154337273228649 1107 54-55 -0.8679208984302562 1107 56-57 -0.7267623727452346 1107 58-59 -0.9335236795388866 1107 60-61 -0.9030994406400197 1107 62-63 -0.8804542060151519 1107 64-65 -0.9092838035873356 1107 66-67 -0.8401723465660851 1107 68-69 -0.7460858248905851 1107 70-71 -0.6776350931013511 1107 72-73 -0.8092426690978058 1107 74-75 -0.8250571210596931 1107 76-77 -1.0273762854666586 1107 78-79 -1.0158592563241413 1107 80-81 -1.0521329769596903 1107 82-83 -1.0250029672562277 1107 84-85 -1.1365567538043422 1107 86-87 -1.1055997054617919 1107 88-89 -0.9790436369717419 1107 90-91 -0.9664201833921453 1107 92-93 -1.036688781999036 1107 94-95 -1.1284365433001469 1107 96-97 -0.9923586485578575 1107 98-99 -1.1637449663049217 1107 100 -0.9187100835096444 1108 1 -0.4359463925507647 1108 2 -0.3849122338991471 1108 3 -0.4930693190174189 1108 4 -0.40352784162879374 1108 5 -0.32535007996696663 1108 6 -0.3342947766888784 1108 7 -0.2741821117946728 1108 8 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50-51 1.042014735489225 2311 52-53 0.8454058816804775 2311 54-55 0.7761400834319332 2311 56-57 0.8280291643456508 2311 58-59 0.7617029589993294 2311 60-61 0.8271913484134217 2311 62-63 0.7960801112535876 2311 64-65 0.7701444310442795 2311 66-67 0.7980993297101975 2311 68-69 0.8408552482489853 2311 70-71 0.8995402040123963 2311 72-73 0.9385475736361357 2311 74-75 0.9655347320560068 2311 76-77 0.9642818362028933 2311 78-79 0.9879703382227518 2311 80-81 1.0022969386549647 2311 82-83 1.065235459175554 2311 84-85 1.071155701439487 2311 86-87 1.2111091608832467 2311 88-89 1.3329026892398268 2311 90-91 1.2313431636131824 2311 92-93 1.1447129267202243 2311 94-95 1.204655603987586 2311 96-97 1.1866245458142082 2311 98-99 1.3031386172147208 2311 100 1.5499795061623942 2312 1 0.5680011177169888 2312 2 0.6187896535075055 2312 3 0.6338521725601716 2312 4 0.4151502761644963 2312 5 0.429662888159136 2312 6 0.31879189097831784 2312 7 0.4314126781259091 2312 8 0.597221781266768 2312 9 0.7615368480576095 2312 10-11 0.6483878656290969 2312 12-13 0.8174311396761738 2312 14-15 0.9015007230099528 2312 16-17 1.0012392173768703 2312 18-19 0.9299635271710898 2312 20-21 0.8936125687222187 2312 22-23 0.9398023758059608 2312 24-25 0.9781393100932547 2312 26-27 0.9839905560560496 2312 28-29 0.9977603823646746 2312 30-31 0.8956376874756558 2312 32-33 1.0195369929566453 2312 34-35 1.037355998239228 2312 36-37 1.0520065809630026 2312 38-39 1.068077705695977 2312 40-41 1.1154854945024013 2312 42-43 1.1440869334309056 2312 44-45 1.160796443075192 2312 46-47 1.1194098991147783 2312 48-49 1.15779164216476 2312 50-51 1.1265875838361694 2312 52-53 1.0708091074869301 2312 54-55 1.0939012599812585 2312 56-57 1.0415807304010656 2312 58-59 1.060590323475303 2312 60-61 1.0284587490291273 2312 62-63 1.0539840063144936 2312 64-65 1.1366423898182063 2312 66-67 1.102468724704174 2312 68-69 1.0710704135548355 2312 70-71 1.1545771803207927 2312 72-73 1.2204233265473974 2312 74-75 1.1721628608205634 2312 76-77 1.0978315618085688 2312 78-79 1.1210540954172359 2312 80-81 0.8963916046969871 2312 82-83 1.1345158433276055 2312 84-85 1.184206999793119 2312 86-87 1.2564235431613895 2312 88-89 1.209977378036509 2312 90-91 1.2133728450480632 2312 92-93 1.4672140979155124 2312 94-95 1.6144559988477134 2312 96-97 1.5404367864781072 2312 98-99 1.4674924520427233 2312 100 1.6630400067647244 2313 1 0.5874235544338546 2313 2 0.5068644653499774 2313 3 0.6112298616234462 2313 4 0.5437738038873903 2313 5 0.4737529303364951 2313 6 0.4978744478699113 2313 7 0.5800325144750076 2313 8 0.6029922871900553 2313 9 0.7177103289547517 2313 10-11 0.5659888828076589 2313 12-13 0.7696403243610703 2313 14-15 0.9356242268072847 2313 16-17 1.003332192145649 2313 18-19 0.9447355164797813 2313 20-21 0.993386642973519 2313 22-23 0.95739826607646 2313 24-25 0.9240746973798863 2313 26-27 0.992595900345016 2313 28-29 1.066610485244631 2313 30-31 1.0467926089656032 2313 32-33 1.124445665634326 2313 34-35 1.1853342874925374 2313 36-37 1.2077218871761275 2313 38-39 1.2193495206897893 2313 40-41 1.1781064040842537 2313 42-43 1.2559940065132693 2313 44-45 1.1847596113676602 2313 46-47 1.0737564917716824 2313 48-49 1.166050578365521 2313 50-51 1.2603641668544796 2313 52-53 1.055982089365024 2313 54-55 1.086188510386286 2313 56-57 1.075230166099388 2313 58-59 1.039795242572687 2313 60-61 1.0562265957961046 2313 62-63 1.0513785116362229 2313 64-65 0.9674351123431038 2313 66-67 0.9877885070498351 2313 68-69 1.0983956372589248 2313 70-71 1.0718880145029104 2313 72-73 1.050842639268275 2313 74-75 0.9246876921452909 2313 76-77 1.1046469315953544 2313 78-79 1.0243792370907983 2313 80-81 1.1571565735595755 2313 82-83 1.158952908822144 2313 84-85 1.1803319117811952 2313 86-87 1.168427343909233 2313 88-89 1.2803903333913915 2313 90-91 1.2799072944523147 2313 92-93 1.420665190630519 2313 94-95 1.4461782298046693 2313 96-97 1.47868591319477 2313 98-99 1.55933314875535 2313 100 1.790699333977667 2314 1 0.4876495313049709 2314 2 0.41096348987585785 2314 3 0.5063650471614665 2314 4 0.4237554443185729 2314 5 0.46909604960502094 2314 6 0.5750480908809834 2314 7 0.6320985887252206 2314 8 0.5617679187168108 2314 9 0.6894712990832588 2314 10-11 0.6974412411740545 2314 12-13 0.4081582049114374 2314 14-15 0.8103805097587369 2314 16-17 0.7186034384486533 2314 18-19 0.7855135528609409 2314 20-21 0.7811688447618081 2314 22-23 0.7010915825066846 2314 24-25 0.68306260510942 2314 26-27 0.819062292639245 2314 28-29 0.7781426609444253 2314 30-31 0.6653705469173943 2314 32-33 0.6331699112391718 2314 34-35 0.7232361597376809 2314 36-37 0.8074404512114342 2314 38-39 0.8206807745736029 2314 40-41 0.8936858312683995 2314 42-43 0.9727611335268804 2314 44-45 1.0070765862600908 2314 46-47 0.9292151140701748 2314 48-49 0.8777083154729226 2314 50-51 0.7799829684802475 2314 52-53 0.6605107830079717 2314 54-55 0.5970143105962009 2314 56-57 0.7306315115252175 2314 58-59 0.6856040162517942 2314 60-61 0.678030839165757 2314 62-63 0.6092542415250222 2314 64-65 0.5182796558752116 2314 66-67 0.5748405170758986 2314 68-69 0.6506982752603392 2314 70-71 0.7628398171001791 2314 72-73 0.6747789860305105 2314 74-75 0.7342756689504242 2314 76-77 0.685813797656678 2314 78-79 0.8199170438662691 2314 80-81 0.9507343768610639 2314 82-83 0.8210669084814803 2314 84-85 0.8520855266368947 2314 86-87 1.0758231127927615 2314 88-89 1.1979925952471824 2314 90-91 1.2540959253886257 2314 92-93 1.4600904627419489 2314 94-95 1.4653300473481217 2314 96-97 1.6345326986544215 2314 98-99 1.6072577163090678 2314 100 1.62545210592247 2315 1 0.49138218696198877 2315 2 0.5448144315010168 2315 3 0.5747000517124228 2315 4 0.45334327643060846 2315 5 0.4527972287760562 2315 6 -0.14781662526601735 2315 7 0.3170570800800263 2315 8 0.33231572540950793 2315 9 0.5926345111991154 2315 10-11 0.6800253092445416 2315 12-13 0.7738579112107189 2315 14-15 0.7952901264917571 2315 16-17 0.7070209134637082 2315 18-19 0.8106378749500394 2315 20-21 0.8660144439912045 2315 22-23 0.7477078764416731 2315 24-25 0.8472078848822591 2315 26-27 0.8339218925630121 2315 28-29 0.9465264106908293 2315 30-31 0.9527433734042319 2315 32-33 0.9767006650408305 2315 34-35 1.0824380166173881 2315 36-37 1.096387344671875 2315 38-39 1.078995365355027 2315 40-41 1.1753089734746212 2315 42-43 1.182100554428139 2315 44-45 1.2259010974087161 2315 46-47 1.2790344510772726 2315 48-49 1.4107852390476339 2315 50-51 1.325032799239132 2315 52-53 1.0555676189675296 2315 54-55 0.8257724551531354 2315 56-57 0.9124634669533691 2315 58-59 0.9698035515465122 2315 60-61 0.9808815190261413 2315 62-63 1.1352316449802515 2315 64-65 1.186438042006408 2315 66-67 1.1420910097857941 2315 68-69 1.216889332482637 2315 70-71 1.2854332071028338 2315 72-73 1.239776247045583 2315 74-75 1.344220433024006 2315 76-77 1.3840006316974893 2315 78-79 1.2909696294647972 2315 80-81 1.3557901256393592 2315 82-83 1.507869644295468 2315 84-85 1.3530419872924213 2315 86-87 1.468463801651513 2315 88-89 1.7053294509183665 2315 90-91 1.8813216135403898 2315 92-93 1.9593974655805937 2315 94-95 1.9572883843620126 2315 96-97 2.0096896685378844 2315 98-99 2.1557951824843045 2315 100 2.213163042545304 2316 1 0.14857185623468894 2316 2 0.1397481548556847 2316 3 0.17231331094914282 2316 4 0.15542540606617905 2316 5 0.11356938169939923 2316 6 0.2573212917483829 2316 7 0.2521769567469434 2316 8 0.2626598876033057 2316 9 0.49958483651151653 2316 10-11 0.36061625855386126 2316 12-13 0.395625372155088 2316 14-15 0.6249593340655295 2316 16-17 0.6073603213421066 2316 18-19 0.5504621513590564 2316 20-21 0.6494544470428991 2316 22-23 0.48459419992305897 2316 24-25 0.4839614410822648 2316 26-27 0.6232452446251884 2316 28-29 0.7016714917421396 2316 30-31 0.707625618036559 2316 32-33 0.7690954816275308 2316 34-35 0.8591594090828636 2316 36-37 0.8832721330024995 2316 38-39 0.8817322135323309 2316 40-41 1.001963935779692 2316 42-43 0.9140978879177766 2316 44-45 0.8918885761608308 2316 46-47 0.9352900043688734 2316 48-49 1.0382816035315088 2316 50-51 0.636472789265305 2316 52-53 0.4033590487325185 2316 54-55 0.43021806569189636 2316 56-57 0.5115772197643196 2316 58-59 0.6088421564015007 2316 60-61 0.6922661971050275 2316 62-63 0.7439535807960596 2316 64-65 0.6492866956791872 2316 66-67 0.743937796157006 2316 68-69 0.732099420190913 2316 70-71 0.6461119949477947 2316 72-73 0.8031779736715166 2316 74-75 0.914353563159203 2316 76-77 0.9778490499804846 2316 78-79 1.0885890758243093 2316 80-81 1.2077494897333878 2316 82-83 1.288194454285673 2316 84-85 1.3376006965748672 2316 86-87 1.3601765926207356 2316 88-89 1.3744714318955253 2316 90-91 1.6018597443594231 2316 92-93 1.7230793804811384 2316 94-95 1.708708631418947 2316 96-97 1.7683788544706935 2316 98-99 1.9739109849541272 2316 100 1.8696777106782712 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 11.0 1 122.0 2 63.0 3 30.0 4 38.0 5 30.0 6 52.0 7 314.0 8 999.0 9 1750.0 10 2059.0 11 2415.0 12 2848.0 13 3150.0 14 3828.0 15 4764.0 16 6474.0 17 9107.0 18 11645.0 19 14657.0 20 18151.0 21 21412.0 22 23206.0 23 23462.0 24 23161.0 25 23986.0 26 26204.0 27 30174.0 28 35798.0 29 42150.0 30 50610.0 31 62369.0 32 77928.0 33 100052.0 34 137090.0 35 188795.0 36 268711.0 37 388189.0 38 369833.0 39 98509.0 40 105.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.27858186170272 18.968392133329274 12.301300272559084 23.451725732408924 2 16.456135624382302 20.979426812882057 39.702663829651634 22.86177373308401 3 19.18058708052502 26.419829169626276 30.468913125140745 23.930670624707957 4 12.86229700132415 17.076529741313216 38.30971625754298 31.751456999819656 5 12.838425968121422 37.404788875434555 34.833564082421546 14.923221074022475 6 29.3733083851038 37.06683684716508 18.96861356436976 14.591241203361355 7 25.909479464954188 33.41267299068378 23.04345896464159 17.634388579720436 8 25.30724667042917 34.850295935053474 21.233328027244355 18.60912936727301 9 26.76281865780772 15.897296400473815 21.068070362267672 36.27181457945079 10-11 24.41859343453587 25.917025428609186 29.219194784824825 20.445186352030113 12-13 25.08814144302132 24.43116816470947 28.558213383801846 21.922477008467368 14-15 22.736901390521002 25.789253394810657 26.835169953906473 24.638675260761865 16-17 21.621501072883767 28.2467632663886 27.65332593967741 22.478409721050223 18-19 21.105021820427083 27.551649472878946 29.978670204032625 21.364658502661342 20-21 22.060235052446185 27.024907265312226 29.78755106887502 21.127306613366574 22-23 22.015716280414395 26.84221198774467 29.482673441000294 21.659398290840638 24-25 21.664308259312133 26.712151901847175 29.837714731000514 21.785825107840175 26-27 21.584529038190627 26.885837500509318 29.61458333584477 21.91505012545528 28-29 21.35200557418708 27.594661124625997 29.556476014359802 21.496857286827126 30-31 22.204812645604964 27.010394232482383 29.618029495771353 21.166763626141304 32-33 21.99814533781037 27.07298042670733 29.307713340402845 21.621160895079456 34-35 22.09072816608469 27.090320679716196 29.562364462559238 21.256586691639875 36-37 21.808687240487444 26.95043857185121 29.520892290398965 21.719981897262382 38-39 21.743628667038134 27.024250377876147 29.589130669076457 21.642990286009265 40-41 21.71133134223565 26.45582441884324 29.97947418914849 21.853370049772618 42-43 21.368964832108087 26.829985204446764 29.495258478507868 22.30579148493728 44-45 20.96853383955451 27.15763754448004 29.78433511687555 22.0894934990899 46-47 20.929577491962036 26.686043314815226 29.53176744549474 22.852611747728 48-49 21.112307486402116 26.797724019933515 29.50953570054874 22.58043279311563 50-51 21.042022366630746 27.052743940150542 29.3509886700846 22.55424502313411 52-53 20.7671223362669 27.30089193429599 29.10400466103555 22.827981068401566 54-55 20.583314403009943 26.958034487431952 29.46375819731233 22.994892912245774 56-57 21.06147824640012 26.85622439210258 28.951351325280285 23.13094603621701 58-59 20.84513129104164 26.852292423269365 29.101077015572702 23.201499270116297 60-61 21.18374639241376 26.780285038492973 28.903293783499016 23.132674785594247 62-63 21.200630365059105 26.48864190374877 28.936418097148163 23.374309634043957 64-65 20.838481102022747 26.39062088600453 28.91606557124078 23.854832440731943 66-67 20.98378348921789 26.29641301960145 28.60959207830453 24.110211412876133 68-69 20.657093368950868 26.39511591573807 28.78977463581689 24.15801607949417 70-71 20.611078381984516 26.08084289866508 28.931403796584043 24.376674922766362 72-73 20.70248471006089 26.221910969278806 28.702014936765092 24.373589383895215 74-75 20.773465423690237 26.128906724687667 28.656716489883937 24.44091136173816 76-77 20.661939092846847 25.823035916332888 28.757031267517142 24.757993723303123 78-79 20.626464660102418 25.838846402360815 29.000752220497045 24.533936717039722 80-81 20.645921772542973 25.890877738888037 28.90954650410609 24.55365398446291 82-83 20.941708187490086 25.511932103387934 29.111284807694254 24.435074901427726 84-85 21.08082879645056 25.61661064421043 28.719353892285003 24.583206667053997 86-87 20.894229522076923 25.653233221775036 28.962011193117682 24.49052606303036 88-89 21.12933215163652 25.0162981579224 29.04543907141004 24.80893061903104 90-91 21.66002879211739 24.813057377130203 28.954714980460082 24.57219885029232 92-93 21.758876513233126 24.7199685412904 28.861581918860736 24.659573026615735 94-95 21.494836772872738 24.434436816561675 28.99301097551949 25.0777154350461 96-97 21.66190150805335 24.172880501009534 28.96269674061222 25.2025212503249 98-99 21.71137072706479 24.21252993257921 28.815474013180342 25.260625327175664 100 22.046826774691358 24.083912037037038 28.784288194444446 25.084972993827158 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1787.0 1 1206.5 2 1434.0 3 2157.0 4 1476.5 5 926.0 6 1229.5 7 1954.0 8 2900.5 9 3338.0 10 2751.0 11 2124.0 12 2062.5 13 2250.5 14 2540.5 15 2799.5 16 2996.5 17 3230.0 18 3567.0 19 3916.5 20 4327.5 21 4741.5 22 5386.0 23 6429.0 24 7556.0 25 8913.0 26 10693.0 27 12709.0 28 15148.5 29 18109.0 30 21817.0 31 25461.0 32 29479.0 33 33846.5 34 38188.0 35 42609.0 36 46928.0 37 52497.0 38 56241.0 39 57653.0 40 59250.5 41 60992.0 42 63233.5 43 66466.0 44 70558.0 45 75683.5 46 80779.0 47 85243.5 48 88866.5 49 90697.0 50 90918.0 51 90260.5 52 87329.0 53 81933.5 54 75513.5 55 67383.0 56 59219.0 57 50149.5 58 41369.5 59 34278.0 60 27049.5 61 20799.5 62 16090.0 63 12377.0 64 9105.0 65 6357.5 66 4543.0 67 3463.5 68 2603.0 69 1860.5 70 1348.0 71 1034.0 72 774.0 73 543.5 74 427.0 75 340.5 76 246.0 77 178.0 78 119.0 79 86.0 80 68.0 81 48.5 82 36.0 83 31.0 84 28.5 85 19.0 86 14.0 87 9.5 88 6.0 89 8.5 90 7.5 91 5.5 92 4.5 93 3.5 94 2.0 95 1.5 96 0.5 97 1.0 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2221524781716388 2 0.1475231300358539 3 0.024008666260737006 4 0.022610571237521398 5 0.027479798732168867 6 0.0558273805821957 7 0.06301069639113106 8 0.055972011101838685 9 0.041798220176825274 10-11 0.04420872883754184 12-13 0.04237674225539725 14-15 0.02044111344287649 16-17 0.019332279458946867 18-19 0.01887428281341072 20-21 0.02210436441877092 22-23 0.02610580879556042 24-25 0.02239362545805691 26-27 0.01950101506519703 28-29 0.019573330325018522 30-31 0.017379767443766448 32-33 0.026901276653596887 34-35 0.027094117346454213 36-37 0.025623707063417107 38-39 0.02359887978841519 40-41 0.025093395158059466 42-43 0.03157766345538703 44-45 0.04093043705896731 46-47 0.046522817151829744 48-49 0.04138843370450346 50-51 0.028034215724133674 52-53 0.04300347450718356 54-55 0.04136432861789629 56-57 0.044160518664327506 58-59 0.047029023970580223 60-61 0.023912245914308346 62-63 0.025647812150024273 64-65 0.02588886301609593 66-67 0.03155355836877986 68-69 0.035699633265212354 70-71 0.040231389547359504 72-73 0.043895362711648685 74-75 0.025021079898237965 76-77 0.018777862466982057 78-79 0.018850177726803554 80-81 0.021188371127698623 82-83 0.018898387900017886 84-85 0.011160655099117706 86-87 0.01436663161787074 88-89 0.01913943876608954 90-91 0.020224167663411997 92-93 0.020320588009840664 94-95 0.024080981520558507 96-97 0.024972869725023633 98-99 0.030492934558064577 100 0.0313848227625297 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2074251.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.0562625033815 #Duplication Level Percentage of deduplicated Percentage of total 1 84.82156143210206 62.8156781936245 2 10.181389385294953 15.079912899330905 3 2.3880380762954725 5.305475239386232 4 0.9424636211857631 2.7918133332168154 5 0.5064421431099462 1.8752606146462638 6 0.30968941030574837 1.3760664158471956 7 0.19374151204322057 1.0043440591572355 8 0.13690855358163773 0.811114862640003 9 0.09347832052826523 0.6230389539074801 >10 0.4066709909127397 4.9014550604357146 >50 0.012278285701334263 0.611989555122959 >100 0.006310125144652556 0.8559233607306076 >500 4.7980043733644723E-4 0.21766027137406596 >1k 4.1125751771690884E-4 0.8018100855000984 >5k 6.85429196194848E-5 0.41988940410590103 >10k+ 6.85429196194848E-5 0.5085676909740113 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10019 0.48301772543438576 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8272 0.3987945528289488 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4714 0.22726275653235795 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2833 0.1365794207162007 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2799 0.13494027482691343 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2348 0.11319748670725 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.821017321433134E-5 2 0.0 0.0 0.0 0.0 4.821017321433134E-5 3 0.0 0.0 0.0 0.0 4.821017321433134E-5 4 0.0 0.0 0.0 0.0 4.821017321433134E-5 5 0.0 0.0 0.0 0.0 4.821017321433134E-5 6 0.0 0.0 0.0 0.0 4.821017321433134E-5 7 0.0 0.0 0.0 0.0 4.821017321433134E-5 8 0.0 0.0 0.0 0.0 9.642034642866268E-5 9 0.0 0.0 0.0 0.0 1.4463051964299403E-4 10-11 0.0 0.0 0.0 2.410508660716567E-5 1.4463051964299403E-4 12-13 0.0 4.821017321433134E-5 0.0 4.821017321433134E-5 1.687356062501597E-4 14-15 0.0 4.821017321433134E-5 0.0 1.687356062501597E-4 1.9284069285732536E-4 16-17 0.0 4.821017321433134E-5 0.0 1.9284069285732536E-4 1.9284069285732536E-4 18-19 0.0 4.821017321433134E-5 0.0 4.338915589289821E-4 1.9284069285732536E-4 20-21 0.0 4.821017321433134E-5 0.0 0.0011570441571439522 1.9284069285732536E-4 22-23 0.0 4.821017321433134E-5 0.0 0.004025549463396667 2.1694577946449102E-4 24-25 0.0 4.821017321433134E-5 0.0 0.010509817760724231 2.410508660716567E-4 26-27 0.0 4.821017321433134E-5 0.0 0.021574052513413274 2.410508660716567E-4 28-29 0.0 4.821017321433134E-5 0.0 0.06141976067505813 2.410508660716567E-4 30-31 4.821017321433134E-5 9.642034642866268E-5 0.0 0.17972752574302725 2.410508660716567E-4 32-33 4.821017321433134E-5 9.642034642866268E-5 0.0 0.4180545170280742 2.410508660716567E-4 34-35 4.821017321433134E-5 1.2052543303582835E-4 0.0 0.7685906864694774 2.410508660716567E-4 36-37 4.821017321433134E-5 1.4463051964299403E-4 0.0 1.2864884722244319 2.410508660716567E-4 38-39 4.821017321433134E-5 1.4463051964299403E-4 0.0 1.975918054275977 2.410508660716567E-4 40-41 4.821017321433134E-5 1.4463051964299403E-4 0.0 2.70338546299363 2.410508660716567E-4 42-43 4.821017321433134E-5 1.4463051964299403E-4 0.0 3.3803286101826635 2.651559526788224E-4 44-45 4.821017321433134E-5 1.4463051964299403E-4 0.0 4.183992197665567 3.374712125003194E-4 46-47 4.821017321433134E-5 1.4463051964299403E-4 0.0 5.08181025343606 4.338915589289821E-4 48-49 4.821017321433134E-5 1.4463051964299403E-4 0.0 5.976301807254764 4.338915589289821E-4 50-51 4.821017321433134E-5 1.4463051964299403E-4 0.0 6.857704299045777 4.338915589289821E-4 52-53 4.821017321433134E-5 1.4463051964299403E-4 0.0 7.694729326392997 4.821017321433134E-4 54-55 4.821017321433134E-5 1.687356062501597E-4 0.0 8.613928593984046 5.303119053576448E-4 56-57 4.821017321433134E-5 1.9284069285732536E-4 0.0 9.606455535034092 5.303119053576448E-4 58-59 4.821017321433134E-5 1.9284069285732536E-4 0.0 10.660185290979733 5.303119053576448E-4 60-61 4.821017321433134E-5 1.9284069285732536E-4 0.0 11.707671829494117 5.785220785719761E-4 62-63 4.821017321433134E-5 1.9284069285732536E-4 0.0 12.722761131608468 5.785220785719761E-4 64-65 4.821017321433134E-5 1.9284069285732536E-4 0.0 13.71584248965048 5.785220785719761E-4 66-67 4.821017321433134E-5 1.9284069285732536E-4 0.0 14.791676610014893 5.785220785719761E-4 68-69 4.821017321433134E-5 1.9284069285732536E-4 0.0 15.930111640298113 5.785220785719761E-4 70-71 7.231525982149701E-5 1.9284069285732536E-4 0.0 17.071752647100084 6.508373383934732E-4 72-73 9.642034642866268E-5 1.9284069285732536E-4 0.0 18.15358893402968 8.436780312507984E-4 74-75 9.642034642866268E-5 1.9284069285732536E-4 0.0 19.186347264627088 8.677831178579642E-4 76-77 9.642034642866268E-5 1.9284069285732536E-4 0.0 20.288938031125454 8.677831178579642E-4 78-79 9.642034642866268E-5 1.9284069285732536E-4 0.0 21.44901942918191 8.677831178579642E-4 80-81 9.642034642866268E-5 1.9284069285732536E-4 0.0 22.577957055341905 8.677831178579642E-4 82-83 9.642034642866268E-5 1.9284069285732536E-4 0.0 23.67833015387241 8.677831178579642E-4 84-85 9.642034642866268E-5 1.9284069285732536E-4 0.0 24.730637709708226 8.677831178579642E-4 86-87 9.642034642866268E-5 1.9284069285732536E-4 0.0 25.784367465653865 8.677831178579642E-4 88 9.642034642866268E-5 1.9284069285732536E-4 0.0 26.59007998549838 8.677831178579642E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3780 0.0 57.19725 1 GTATCAA 6905 0.0 42.08969 1 TACCTGG 1445 0.0 38.42716 2 CAACGCA 8345 0.0 34.64186 5 ATCAACG 8360 0.0 34.635933 3 TATCAAC 8455 0.0 34.061356 2 TCAACGC 8680 0.0 33.30488 4 AACGCAG 8860 0.0 32.842052 6 ACCTGGG 1740 0.0 31.337273 3 GTGGTAT 1435 0.0 31.183577 1 ACGCAGA 9805 0.0 29.29392 7 CGCAGAG 9980 0.0 28.778164 8 TAACGCA 175 5.5515557E-9 26.86052 4 ATAACGC 175 5.5515557E-9 26.86052 3 GCAGAGT 10875 0.0 25.847847 9 GTACATG 6295 0.0 25.590845 1 CGTTAAC 205 1.0550139E-9 25.275112 1 TACATGG 6425 0.0 24.169346 2 TGGTATC 1755 0.0 24.131786 2 ATGGGGG 3675 0.0 23.279116 5 >>END_MODULE