FastQCFastQC Report
Mon 6 Jun 2016
ERR1211444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211444_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074251
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89880.43331303685041006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78020.37613577141821314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47620.22957684484664587No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29390.1416896990769198No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22500.10847288973224552No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20860.10056642132509518No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37350.060.1773761
TACCTGG16300.042.3823282
GTATCAA68350.041.827421
GTGGTAT13250.033.713491
ATCAACG85100.033.246273
CAACGCA85150.033.1667565
TATCAAC87650.032.867272
ACCTGGG20900.032.6060183
TCAACGC88950.031.755214
AACGCAG91750.031.3955126
GTACATG61650.028.449311
ACGCAGA102000.027.733817
TACATGG62900.027.5696222
TGGTATC15600.027.1127172
CGCAGAG103750.027.0395478
ACATGGG63250.026.2295113
ATGGGGG36450.026.0415635
GTACTAG7950.025.4329831
TGGGGGG37300.025.0701796
CATGGGG49400.024.9264744
Produced by FastQC (version 0.11.5)