FastQCFastQC Report
Mon 6 Jun 2016
ERR1211443_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211443_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3793716
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166560.4390418260091161No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143990.37954870633436977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70330.18538551646986753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70250.054.6461221
GTATCAA127050.040.4110261
CAACGCA145700.034.2329145
ATCAACG145800.034.2094353
TATCAAC154250.033.1004452
AACGCAG158300.031.7164136
TCAACGC161350.031.0577894
GTACTAG15850.030.0151251
GTGGTAT26300.029.013981
ACGCAGA172100.028.6272667
CGCAGAG176000.027.8853368
GTACATG124700.025.7989651
GCAGAGT188100.025.3917689
TAGGCAT22650.025.113385
TACATGG126350.024.878862
TCTAACG8800.024.0635192
CTAACGC8650.023.912483
CTGTGCG20500.023.8488549
ACATGGG126200.023.728383
GAGTACT121100.023.69538112-13
Produced by FastQC (version 0.11.5)