##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211443_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3793716 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83616986616816 31.0 31.0 34.0 30.0 34.0 2 31.923804259464863 33.0 31.0 34.0 30.0 34.0 3 31.990035363743623 33.0 31.0 34.0 30.0 34.0 4 35.64300596038291 37.0 35.0 37.0 33.0 37.0 5 35.69144158392457 37.0 35.0 37.0 35.0 37.0 6 35.766586639590315 37.0 35.0 37.0 35.0 37.0 7 35.71666566501025 37.0 35.0 37.0 35.0 37.0 8 35.7256776732892 37.0 35.0 37.0 35.0 37.0 9 37.37103067282843 39.0 37.0 39.0 35.0 39.0 10-11 37.31423055916679 39.0 37.0 39.0 34.5 39.0 12-13 37.245970968833724 39.0 37.0 39.0 34.0 39.0 14-15 38.51132517563255 40.0 38.0 41.0 34.0 41.0 16-17 38.50466995948036 40.0 38.0 41.0 34.0 41.0 18-19 38.504848280683106 40.0 38.0 41.0 34.0 41.0 20-21 38.49458907836011 40.0 38.0 41.0 34.0 41.0 22-23 38.38026001419189 40.0 38.0 41.0 34.0 41.0 24-25 38.29317481856839 40.0 38.0 41.0 34.0 41.0 26-27 38.15958469215935 40.0 38.0 41.0 34.0 41.0 28-29 38.043414293531725 40.0 38.0 41.0 33.0 41.0 30-31 37.88278432017579 40.0 37.0 41.0 33.0 41.0 32-33 37.691450018926034 40.0 37.0 41.0 33.0 41.0 34-35 37.56772502211552 40.0 37.0 41.0 33.0 41.0 36-37 37.43357581326594 40.0 36.0 41.0 32.5 41.0 38-39 37.27394775992721 39.0 36.0 41.0 32.0 41.0 40-41 37.099877929713244 39.0 36.0 41.0 31.0 41.0 42-43 36.88803576756932 39.0 35.5 40.5 31.0 41.0 44-45 36.720220754531965 39.0 35.0 40.0 30.5 41.0 46-47 36.453708316595126 39.0 35.0 40.0 30.0 41.0 48-49 36.181108179948104 39.0 35.0 40.0 29.0 41.0 50-51 35.50147849759972 38.0 34.0 39.5 28.5 40.5 52-53 35.79361343864433 38.5 35.0 40.0 29.0 40.5 54-55 36.18057018501121 39.0 35.0 40.0 29.0 41.0 56-57 36.07573656014314 39.0 35.0 40.0 29.0 41.0 58-59 35.85190997428379 38.0 35.0 40.0 29.0 41.0 60-61 35.622054339333786 38.0 35.0 40.0 28.0 41.0 62-63 35.34517567999291 37.5 34.0 40.0 28.0 41.0 64-65 35.13022680137364 37.0 34.0 40.0 28.0 41.0 66-67 34.92369987632179 36.5 34.0 40.0 28.0 41.0 68-69 34.589777542652115 36.0 34.0 39.0 27.0 41.0 70-71 34.2401151008668 35.5 34.0 39.0 26.0 41.0 72-73 33.87773768516146 35.0 33.5 38.5 26.0 40.5 74-75 33.51193355011287 35.0 33.0 37.5 26.0 40.0 76-77 33.14407417423972 35.0 33.0 37.0 25.0 39.0 78-79 32.75720942210751 35.0 33.0 36.5 24.5 39.0 80-81 32.376571282615785 35.0 32.0 36.0 24.0 38.5 82-83 32.027239387450194 35.0 32.0 36.0 23.0 37.0 84-85 31.71086791947526 35.0 31.5 35.0 21.5 37.0 86-87 31.39431140865579 35.0 31.0 35.0 20.0 36.5 88-89 31.088539442594016 34.0 31.0 35.0 19.5 36.0 90-91 30.777478467022835 34.0 31.0 35.0 18.5 36.0 92-93 30.487228748804604 34.0 30.0 35.0 18.0 35.5 94-95 30.210356679308624 34.0 30.0 35.0 16.0 35.0 96-97 29.92198256274323 34.0 29.0 35.0 15.0 35.0 98-99 29.600525711466013 34.0 29.0 35.0 10.5 35.0 100 28.31650339666965 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3196612683160964 1101 2 -0.3579924865309323 1101 3 -0.3466750270211172 1101 4 -0.19685533884534578 1101 5 -0.16216390088741406 1101 6 -0.17369943471508975 1101 7 -0.16878119569983596 1101 8 -0.14352063748299315 1101 9 -0.2993645446119473 1101 10-11 -0.30003539806166657 1101 12-13 -0.33246384668620976 1101 14-15 -0.5009619609154754 1101 16-17 -0.4144231821281892 1101 18-19 -0.4969818919269713 1101 20-21 -0.5103455050959482 1101 22-23 -0.5580553981652585 1101 24-25 -0.653905287406559 1101 26-27 -0.636648616602919 1101 28-29 -0.5989920936138731 1101 30-31 -0.5694088607827439 1101 32-33 -0.567914823619013 1101 34-35 -0.6642055550306409 1101 36-37 -0.7588243019653262 1101 38-39 -0.8468780044190893 1101 40-41 -0.950698903702694 1101 42-43 -0.8318053109550121 1101 44-45 -0.8372457679806331 1101 46-47 -0.844534784177867 1101 48-49 -0.9699862320891626 1101 50-51 -0.9714986466967446 1101 52-53 -0.6559626201939395 1101 54-55 -0.5421658210941587 1101 56-57 -0.6015420954310002 1101 58-59 -0.5950875862730811 1101 60-61 -0.638720288334433 1101 62-63 -0.6693751020726921 1101 64-65 -0.6834939314735138 1101 66-67 -0.7193315380983449 1101 68-69 -0.8131014903085898 1101 70-71 -0.7544385550117099 1101 72-73 -0.7629510462566671 1101 74-75 -0.8227597295800493 1101 76-77 -0.8801004996971002 1101 78-79 -0.9289989662758487 1101 80-81 -1.1181260659400039 1101 82-83 -1.1424486360714106 1101 84-85 -1.0902080371021903 1101 86-87 -1.209762965763666 1101 88-89 -1.2494102430183176 1101 90-91 -1.4348358937652996 1101 92-93 -1.6546708492694648 1101 94-95 -1.728036440703253 1101 96-97 -1.7299690925546223 1101 98-99 -1.9606971154333834 1101 100 -1.847121857153926 1102 1 -0.439618603138058 1102 2 -0.42680424138559303 1102 3 -0.41920662716466595 1102 4 -0.40964372753708034 1102 5 -0.35836630130210523 1102 6 -0.3106458839355568 1102 7 -0.3358596278542265 1102 8 -0.342935685929902 1102 9 -0.5080798043910448 1102 10-11 -0.4163506418911709 1102 12-13 -0.4938817660609871 1102 14-15 -0.6632291964511836 1102 16-17 -0.7338852818929311 1102 18-19 -0.8081888778514426 1102 20-21 -0.7792553499861015 1102 22-23 -0.7376217684352042 1102 24-25 -0.7893157902176782 1102 26-27 -0.8842252743643897 1102 28-29 -0.8598449984346388 1102 30-31 -0.8229291367108118 1102 32-33 -0.822209651961451 1102 34-35 -0.8791870136214897 1102 36-37 -0.8655773622946796 1102 38-39 -0.9892148200815924 1102 40-41 -1.0778728340826973 1102 42-43 -0.9574941342733254 1102 44-45 -1.0341760682318437 1102 46-47 -1.1570233204033968 1102 48-49 -1.238187914771892 1102 50-51 -0.9650809266919609 1102 52-53 -0.7733658260633902 1102 54-55 -0.723423048256322 1102 56-57 -0.7859134818499172 1102 58-59 -0.7968025669341969 1102 60-61 -0.7964466729590214 1102 62-63 -0.7927139513091035 1102 64-65 -0.9555328883697243 1102 66-67 -1.0591688521858273 1102 68-69 -0.9816618396048042 1102 70-71 -0.9511182471844464 1102 72-73 -0.9288775186040468 1102 74-75 -1.0356029450192565 1102 76-77 -1.0046965760956823 1102 78-79 -0.9211968210547425 1102 80-81 -1.0802746763311646 1102 82-83 -1.1002180846140135 1102 84-85 -1.2876142335215057 1102 86-87 -1.265490666230189 1102 88-89 -1.445387355343371 1102 90-91 -1.4725293823414027 1102 92-93 -1.5010215809573317 1102 94-95 -1.5009781449179336 1102 96-97 -1.6913359732117463 1102 98-99 -1.746909484347615 1102 100 -1.7318457297166283 1103 1 -0.34522846269771534 1103 2 -0.34212572463750845 1103 3 -0.45537795921173085 1103 4 -0.2689753245613318 1103 5 -0.23390813768462237 1103 6 -0.3269990419047133 1103 7 -0.3521371453883688 1103 8 -0.3505757499696003 1103 9 -0.42266692924869886 1103 10-11 -0.4682491027793674 1103 12-13 -0.511342353213216 1103 14-15 -0.6184975717554551 1103 16-17 -0.6369524445175827 1103 18-19 -0.6695137333744157 1103 20-21 -0.6328430252324395 1103 22-23 -0.59282963139146 1103 24-25 -0.6931404975005933 1103 26-27 -0.6111982733898031 1103 28-29 -0.7485431391243296 1103 30-31 -0.7869571685238839 1103 32-33 -0.7410130658917566 1103 34-35 -0.8085044480195691 1103 36-37 -0.7920274920617416 1103 38-39 -0.7594193685424031 1103 40-41 -0.8201396448842928 1103 42-43 -0.9693107666547363 1103 44-45 -0.8324082486376909 1103 46-47 -0.9461020579151196 1103 48-49 -0.820568900819076 1103 50-51 -0.9248018248898191 1103 52-53 -0.7355954020056217 1103 54-55 -0.6997958651365295 1103 56-57 -0.5976963231023049 1103 58-59 -0.5895840747863517 1103 60-61 -0.6171887246317027 1103 62-63 -0.6335214338585899 1103 64-65 -0.612877536599882 1103 66-67 -0.6598578643293465 1103 68-69 -0.660367688777022 1103 70-71 -0.6733057533611131 1103 72-73 -0.7496347743842691 1103 74-75 -0.802653591421894 1103 76-77 -0.8735373921305438 1103 78-79 -0.8884952564000379 1103 80-81 -0.7246441391852514 1103 82-83 -0.8087353876485999 1103 84-85 -0.9134086481405603 1103 86-87 -1.080156966477869 1103 88-89 -1.122609663722205 1103 90-91 -1.1877851458384647 1103 92-93 -1.2904249261650733 1103 94-95 -1.114224989685514 1103 96-97 -1.281886126241087 1103 98-99 -1.560553878517144 1103 100 -1.679123206199673 1104 1 -0.3733320271786873 1104 2 -0.3851840446525969 1104 3 -0.4076364631651259 1104 4 -0.24075172680917234 1104 5 -0.2850988527072218 1104 6 -0.23663202186310173 1104 7 -0.2872094771746063 1104 8 -0.32904076675171723 1104 9 -0.42349326158043965 1104 10-11 -0.38377207215078357 1104 12-13 -0.5108088720079067 1104 14-15 -0.6143258050836025 1104 16-17 -0.5663231959221022 1104 18-19 -0.5297657018073565 1104 20-21 -0.6247797573670226 1104 22-23 -0.61020054960278 1104 24-25 -0.5721892253733287 1104 26-27 -0.6427854325723317 1104 28-29 -0.7345540972791156 1104 30-31 -0.7772611476174305 1104 32-33 -0.7799648955444454 1104 34-35 -0.811539091988486 1104 36-37 -0.8256224378157171 1104 38-39 -0.7777723430006844 1104 40-41 -0.8368519767907543 1104 42-43 -0.7968392045841952 1104 44-45 -0.8870574809882967 1104 46-47 -0.8648576604070044 1104 48-49 -0.8375944650881593 1104 50-51 -0.7171515916984177 1104 52-53 -0.666815774074955 1104 54-55 -0.6262225141006397 1104 56-57 -0.6086814337170097 1104 58-59 -0.5119019792421184 1104 60-61 -0.6464012349913872 1104 62-63 -0.7128923543240546 1104 64-65 -0.6527309307049975 1104 66-67 -0.5325528122623311 1104 68-69 -0.6597840528245982 1104 70-71 -0.5788107346479521 1104 72-73 -0.5775087541014656 1104 74-75 -0.6527198257433682 1104 76-77 -0.7801790265065662 1104 78-79 -0.7365578367509471 1104 80-81 -0.8055075860298615 1104 82-83 -0.7324480449034567 1104 84-85 -0.8014766835246405 1104 86-87 -0.9081335098352348 1104 88-89 -0.9296845449474986 1104 90-91 -1.0113092813547162 1104 92-93 -1.0705715921705234 1104 94-95 -1.0627831252363258 1104 96-97 -1.1445435501580299 1104 98-99 -1.2742636316806504 1104 100 -1.1886409617315365 1105 1 -0.4410796082082804 1105 2 -0.3894264336196649 1105 3 -0.4312035077610723 1105 4 -0.33073798804727517 1105 5 -0.2988318122998166 1105 6 -0.34222058103016195 1105 7 -0.3435823715058106 1105 8 -0.35489119828881854 1105 9 -0.3803632136915098 1105 10-11 -0.3787080682327755 1105 12-13 -0.48318651733606544 1105 14-15 -0.6213372517551008 1105 16-17 -0.6510435059535737 1105 18-19 -0.6222431449071806 1105 20-21 -0.6546973755657959 1105 22-23 -0.6005277504474478 1105 24-25 -0.6319090503686695 1105 26-27 -0.7011118439413622 1105 28-29 -0.6304123963743393 1105 30-31 -0.7164258754994677 1105 32-33 -0.7807039636912094 1105 34-35 -0.681894780320043 1105 36-37 -0.6081290157216799 1105 38-39 -0.8030559168847375 1105 40-41 -0.7325602773274937 1105 42-43 -0.6479972986224638 1105 44-45 -0.7459356730927027 1105 46-47 -0.8760270906917214 1105 48-49 -0.7646348307927155 1105 50-51 -0.7794109208472051 1105 52-53 -0.6495287688548714 1105 54-55 -0.544120311275023 1105 56-57 -0.509005782367872 1105 58-59 -0.4698729633831604 1105 60-61 -0.4556569212014665 1105 62-63 -0.5509728768503095 1105 64-65 -0.5146579602367112 1105 66-67 -0.46589393337213636 1105 68-69 -0.4677415194164851 1105 70-71 -0.7214442233922966 1105 72-73 -0.6311379304665863 1105 74-75 -0.5294564137689051 1105 76-77 -0.7912069350325623 1105 78-79 -0.7120017717412388 1105 80-81 -0.7324545256150294 1105 82-83 -0.8223086500508607 1105 84-85 -0.8133191354466796 1105 86-87 -1.0089737012168989 1105 88-89 -0.8008523288291762 1105 90-91 -0.9070437375784373 1105 92-93 -0.8672203279125021 1105 94-95 -1.0784412703167128 1105 96-97 -1.075591130299145 1105 98-99 -0.9136358643681675 1105 100 -0.9946586010905776 1106 1 -0.5011568694891153 1106 2 -0.5711009909297609 1106 3 -0.5121588979888614 1106 4 -0.38638613063420735 1106 5 -0.4455656604182039 1106 6 -0.4640806663866428 1106 7 -0.46010084379367555 1106 8 -0.4396203442278619 1106 9 -0.6022384449052041 1106 10-11 -0.6762963951291212 1106 12-13 -0.5966134068035132 1106 14-15 -0.719070465314303 1106 16-17 -0.7701709827146956 1106 18-19 -0.7862487540970591 1106 20-21 -0.8825889068554815 1106 22-23 -0.8239791518309474 1106 24-25 -0.8455915044153599 1106 26-27 -0.9189799949020383 1106 28-29 -0.7847545569569832 1106 30-31 -0.9110217640085452 1106 32-33 -0.9227975215662312 1106 34-35 -0.849264206057029 1106 36-37 -0.8452319265987498 1106 38-39 -0.9748398812519312 1106 40-41 -0.9855376133801101 1106 42-43 -0.9793859561163103 1106 44-45 -0.9452662958398719 1106 46-47 -0.761323640482857 1106 48-49 -0.838300015080371 1106 50-51 -0.8811760660515873 1106 52-53 -0.8223702448273684 1106 54-55 -0.7437054105369683 1106 56-57 -0.8644453212374472 1106 58-59 -0.9303955041616447 1106 60-61 -0.7454798191262242 1106 62-63 -0.7864132252398477 1106 64-65 -0.7497989168107537 1106 66-67 -0.7509151158109546 1106 68-69 -0.6758580885490559 1106 70-71 -0.7495851825776967 1106 72-73 -0.6612794920044678 1106 74-75 -0.604152691456882 1106 76-77 -0.7315814381135297 1106 78-79 -0.7353626026394835 1106 80-81 -0.7204721069957216 1106 82-83 -0.7377565539599722 1106 84-85 -0.7504719667209621 1106 86-87 -0.7844843733882989 1106 88-89 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0.8417246964603251 2311 18-19 0.8747192806574375 2311 20-21 0.8506172682615016 2311 22-23 0.9458282480171576 2311 24-25 0.9931289512359527 2311 26-27 0.9462277788821893 2311 28-29 1.0036072265475866 2311 30-31 1.0812868772052937 2311 32-33 1.0629475501962418 2311 34-35 1.0982153075145078 2311 36-37 1.1341826471907055 2311 38-39 1.1966275843745393 2311 40-41 1.240591624474284 2311 42-43 1.2738484168491482 2311 44-45 1.2335582164762826 2311 46-47 1.3066259784474568 2311 48-49 1.320278678784824 2311 50-51 1.2395586667074525 2311 52-53 1.1140767515036742 2311 54-55 1.01753678381138 2311 56-57 1.0613806620270054 2311 58-59 1.0509500241492802 2311 60-61 1.053840311184473 2311 62-63 0.9694019424115083 2311 64-65 0.9620455190360246 2311 66-67 0.9956866571770533 2311 68-69 1.0007149258732184 2311 70-71 0.9762692103617567 2311 72-73 0.9970583426425037 2311 74-75 0.9765194154133425 2311 76-77 1.001194106140467 2311 78-79 1.043532746958391 2311 80-81 1.1288016395363982 2311 82-83 1.204593175369343 2311 84-85 1.2004058050135278 2311 86-87 1.2616741525981432 2311 88-89 1.31760607447724 2311 90-91 1.3323519938694481 2311 92-93 1.3559936723421337 2311 94-95 1.3618654213141959 2311 96-97 1.3447599657456415 2311 98-99 1.4920540376193792 2311 100 1.6314737143010802 2312 1 0.4487061118087077 2312 2 0.47983522654099886 2312 3 0.5124977545170495 2312 4 0.3513940124023591 2312 5 0.33639807104854214 2312 6 0.1421841896251479 2312 7 0.272349090022189 2312 8 0.3769056080197828 2312 9 0.5267089964576286 2312 10-11 0.5406957102037353 2312 12-13 0.6344553760306226 2312 14-15 0.7856857682821001 2312 16-17 0.8380996004263039 2312 18-19 0.733709410388208 2312 20-21 0.7428400888765765 2312 22-23 0.7751991363253268 2312 24-25 0.7830643448880963 2312 26-27 0.8099146901027794 2312 28-29 0.8658272720656015 2312 30-31 0.8543744999912946 2312 32-33 0.9338910791799364 2312 34-35 0.8666587591890647 2312 36-37 0.903875896556265 2312 38-39 0.9824965170625717 2312 40-41 0.9688112290289723 2312 42-43 1.0729586990455928 2312 44-45 1.0228582695284274 2312 46-47 1.0115050488742838 2312 48-49 1.0540494814706065 2312 50-51 1.065258675904225 2312 52-53 0.9125964721626403 2312 54-55 0.835969532687578 2312 56-57 0.8669017798730536 2312 58-59 0.8327942628911629 2312 60-61 0.755327417826507 2312 62-63 0.6922281799702503 2312 64-65 0.786271433623277 2312 66-67 0.7107125025274357 2312 68-69 0.7713782543913013 2312 70-71 0.8331904670831349 2312 72-73 0.8272995110266308 2312 74-75 0.9060417894827708 2312 76-77 0.8921376110278061 2312 78-79 0.9143074006435441 2312 80-81 0.8196875541316828 2312 82-83 1.012516097550563 2312 84-85 1.0339865276070377 2312 86-87 1.0793018823639322 2312 88-89 1.1209840222146568 2312 90-91 1.174624473059236 2312 92-93 1.2053687534163338 2312 94-95 1.3100439978058738 2312 96-97 1.3302210274391442 2312 98-99 1.1200874174988407 2312 100 1.4599313506975022 2313 1 0.6118649543903025 2313 2 0.6389875480336826 2313 3 0.6843328609733916 2313 4 0.5414864339024064 2313 5 0.49845745520207885 2313 6 0.5323847442408436 2313 7 0.5498145664364458 2313 8 0.5662288026913131 2313 9 0.7489757487165392 2313 10-11 0.657082054378364 2313 12-13 0.780326909633267 2313 14-15 1.0473889909563283 2313 16-17 0.9560464662407568 2313 18-19 0.9252202908889018 2313 20-21 1.0388852305116458 2313 22-23 1.1068089996102302 2313 24-25 1.124107482671512 2313 26-27 1.101294620769906 2313 28-29 1.108613701674848 2313 30-31 1.1398547919034172 2313 32-33 1.17435820320555 2313 34-35 1.1088328022760123 2313 36-37 1.1487089345332961 2313 38-39 1.147194601443445 2313 40-41 1.1526038325504757 2313 42-43 1.186663389240671 2313 44-45 1.0991886634852648 2313 46-47 1.1011952419809532 2313 48-49 1.2592970901485288 2313 50-51 1.226063337607961 2313 52-53 1.0304662970728984 2313 54-55 1.0289530569680778 2313 56-57 0.9648918989337574 2313 58-59 0.9197084219847653 2313 60-61 0.9127730172934108 2313 62-63 0.9645293543416003 2313 64-65 0.9769644195119795 2313 66-67 0.9367741596831962 2313 68-69 0.970914393274569 2313 70-71 0.9300257607232538 2313 72-73 0.9676573985374617 2313 74-75 0.9712578015381936 2313 76-77 0.9987210419922619 2313 78-79 1.0296553674138877 2313 80-81 1.0661957884447943 2313 82-83 1.090292929557652 2313 84-85 1.226004380605115 2313 86-87 1.265053216393195 2313 88-89 1.3708276744973347 2313 90-91 1.383202967888824 2313 92-93 1.44833428835015 2313 94-95 1.5595855945741697 2313 96-97 1.639173180505825 2313 98-99 1.6341799918984812 2313 100 1.7510230183394953 2314 1 0.48621287526754386 2314 2 0.49031583020452274 2314 3 0.49862322147480853 2314 4 0.40107974804540447 2314 5 0.39053234764957523 2314 6 0.4231681176281228 2314 7 0.4397578486729685 2314 8 0.48449925259455995 2314 9 0.59150463671903 2314 10-11 0.633791320075531 2314 12-13 0.3714606107171292 2314 14-15 0.7350059863546008 2314 16-17 0.7971020432265234 2314 18-19 0.7598278929508169 2314 20-21 0.8002688902278905 2314 22-23 0.748469519187573 2314 24-25 0.6171802139178553 2314 26-27 0.7935063083472897 2314 28-29 0.8103363596006403 2314 30-31 0.6765828710776631 2314 32-33 0.7517648201939124 2314 34-35 0.8587490386464296 2314 36-37 0.983099580501154 2314 38-39 1.0139537033487116 2314 40-41 1.009188025381583 2314 42-43 1.1461063335697403 2314 44-45 1.0951669477062467 2314 46-47 1.1138478294577183 2314 48-49 1.1300597286371712 2314 50-51 1.005528861294195 2314 52-53 0.8747093372607324 2314 54-55 0.809095788865946 2314 56-57 0.8612978188760323 2314 58-59 0.8684609377134223 2314 60-61 0.8547640001469716 2314 62-63 0.8342173635166574 2314 64-65 0.865342300499826 2314 66-67 0.8320314729281222 2314 68-69 0.854573711432657 2314 70-71 0.8762221090403628 2314 72-73 0.8340052790754626 2314 74-75 0.9328920024172405 2314 76-77 0.918014768298768 2314 78-79 0.9750049898373092 2314 80-81 0.9819338233162043 2314 82-83 0.871354639699792 2314 84-85 0.920616518177301 2314 86-87 1.1972595457521855 2314 88-89 1.358872133113593 2314 90-91 1.3218976777561586 2314 92-93 1.4478524733180258 2314 94-95 1.354870070456684 2314 96-97 1.49337443906348 2314 98-99 1.5740510416886444 2314 100 1.4172503323288375 2315 1 0.43481081683540523 2315 2 0.4258552358294949 2315 3 0.44404598761939695 2315 4 0.37347634963935405 2315 5 0.4039111640229365 2315 6 -0.3035413389332433 2315 7 0.19328234167628722 2315 8 0.27415387280071 2315 9 0.48351353268234476 2315 10-11 0.5832222858283274 2315 12-13 0.6027580039724114 2315 14-15 0.782640189622164 2315 16-17 0.6549373659734385 2315 18-19 0.7491103011592273 2315 20-21 0.7943809878420751 2315 22-23 0.6987810549429625 2315 24-25 0.7730485480813911 2315 26-27 0.8623580171667768 2315 28-29 0.9258561996857466 2315 30-31 0.9301796551831671 2315 32-33 1.0091280511957663 2315 34-35 1.0193743016908101 2315 36-37 1.0214341839582985 2315 38-39 1.0353079450392002 2315 40-41 1.0914800449535633 2315 42-43 1.1362978795075804 2315 44-45 1.2043130999285054 2315 46-47 1.3024830780626573 2315 48-49 1.2778192543646227 2315 50-51 1.2606003704722113 2315 52-53 1.042616352931141 2315 54-55 0.8929085369390961 2315 56-57 0.8955699038077469 2315 58-59 0.7576057514102601 2315 60-61 0.7476660064061278 2315 62-63 0.9904065816541845 2315 64-65 1.0242126601554347 2315 66-67 1.0213409467105947 2315 68-69 1.0584555424388569 2315 70-71 1.0982892429301856 2315 72-73 1.1096691568345634 2315 74-75 1.0941439448214894 2315 76-77 1.0480425205712365 2315 78-79 1.047470046679031 2315 80-81 1.0319364654956402 2315 82-83 1.2499796226999855 2315 84-85 1.245031825328649 2315 86-87 1.2423822702925058 2315 88-89 1.4657655926599489 2315 90-91 1.5542203573391937 2315 92-93 1.6340274773349428 2315 94-95 1.5899418907949219 2315 96-97 1.6849896502916444 2315 98-99 1.7881849312476916 2315 100 1.872702918888379 2316 1 -0.009095612025465272 2316 2 -0.07216813267923072 2316 3 0.09236431025570013 2316 4 0.06151092870803154 2316 5 0.06991677490508152 2316 6 0.19482407303460292 2316 7 0.18544584280699183 2316 8 0.17696871306734607 2316 9 0.2915521453117478 2316 10-11 0.10107110324185697 2316 12-13 0.24649608769494336 2316 14-15 0.40158230117874183 2316 16-17 0.5229932457285074 2316 18-19 0.39678935421860473 2316 20-21 0.35243407273794247 2316 22-23 0.2066127297234388 2316 24-25 0.3026849487260179 2316 26-27 0.4193641191722932 2316 28-29 0.5412263068925611 2316 30-31 0.5259010929981685 2316 32-33 0.542959697342944 2316 34-35 0.5665509336800625 2316 36-37 0.6309912641334208 2316 38-39 0.6445369871743409 2316 40-41 0.6817407698367077 2316 42-43 0.7271595924886114 2316 44-45 0.6176541020069095 2316 46-47 0.6474141433895326 2316 48-49 0.7620012227298005 2316 50-51 0.43779179840873184 2316 52-53 0.3092151088764368 2316 54-55 0.11797157688690874 2316 56-57 0.1904270827677479 2316 58-59 0.28467464749067517 2316 60-61 0.3725118689874449 2316 62-63 0.3975514306788739 2316 64-65 0.3993250925481604 2316 66-67 0.3676772224268774 2316 68-69 0.42142838873218125 2316 70-71 0.31288369890057055 2316 72-73 0.3898975198624939 2316 74-75 0.5607596940087092 2316 76-77 0.6079404304024862 2316 78-79 0.7018614302961836 2316 80-81 0.8409414520473248 2316 82-83 0.9249032620423954 2316 84-85 0.9343615944384247 2316 86-87 1.0484521878348225 2316 88-89 1.1429764725675184 2316 90-91 1.2038903585405514 2316 92-93 1.244007209937255 2316 94-95 1.2702493688795506 2316 96-97 1.4221827837769823 2316 98-99 1.6646453074270262 2316 100 1.5528244361296721 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 6.0 1 179.0 2 92.0 3 65.0 4 62.0 5 49.0 6 56.0 7 305.0 8 1162.0 9 2092.0 10 2776.0 11 3110.0 12 3414.0 13 3431.0 14 3942.0 15 4737.0 16 6594.0 17 9415.0 18 13015.0 19 17796.0 20 23871.0 21 30163.0 22 32379.0 23 31171.0 24 28371.0 25 28062.0 26 30884.0 27 36343.0 28 45031.0 29 56996.0 30 72729.0 31 96136.0 32 129619.0 33 179574.0 34 261971.0 35 380641.0 36 570250.0 37 794140.0 38 706132.0 39 186824.0 40 131.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.76037371249748 19.871331628108944 12.659178048035768 23.709116611357807 2 16.049624506283568 20.85375857965925 39.72172394533935 23.37489296871783 3 18.994576902704587 26.28062636786945 30.359721486554577 24.36507524287139 4 12.62292730718407 17.081319478645845 39.04316284249591 31.25259037167417 5 12.770372956218276 37.843222971141586 35.51908865501418 13.867315417625958 6 29.4890729247049 38.00669554672826 18.25736449482485 14.246867033741989 7 25.716312030550124 33.23927302343455 23.245204651725857 17.79921029428947 8 25.30884367735155 34.92336763098313 20.916766553132344 18.85102213853297 9 26.115229308639442 15.457379226616833 21.258610000878107 37.16878146386562 10-11 24.449706182973486 26.10482005487116 28.720601156703466 20.724872605451885 12-13 25.116237920670255 24.391834127854327 28.571599974895985 21.92032797657943 14-15 22.564359583715838 25.701188984088 26.461091604345494 25.273359827850673 16-17 21.457920187795903 28.51367262369584 27.441172733106356 22.5872344554019 18-19 20.978559337997332 27.880861981760813 29.860669244771387 21.279909435470465 20-21 21.87129950043297 27.213280201909605 29.645388743349727 21.270031554307696 22-23 22.12805914648679 26.95687296856643 29.3173697720576 21.597698112889177 24-25 21.73344659517145 27.037599186497374 29.684158713159913 21.544795505171262 26-27 21.615914540755295 27.256703987902114 29.364118859722605 21.763262611619986 28-29 21.295899739089524 27.693802120634153 29.343128581949657 21.667169558326666 30-31 21.757996375020596 27.377637172516067 29.418658757620697 21.445707694842643 32-33 21.834190429611546 27.568447959249585 28.921666980854745 21.675694630284127 34-35 21.90618011899553 27.36978956223075 29.425997437534463 21.298032881239255 36-37 21.739360270971478 27.008072907294643 29.41909579588974 21.83347102584414 38-39 21.701428207217784 27.03557955220453 29.66354305383928 21.599449186738404 40-41 21.502081713259965 26.641816789986684 29.998211135804848 21.857890360948502 42-43 21.2923028733266 26.860336365082926 29.516469096047675 22.330891665542797 44-45 21.14492582797977 27.385609333357593 29.554933598921824 21.914531239740814 46-47 21.21525327544542 27.06178226845098 29.270433494824584 22.45253096127902 48-49 21.374428359813752 27.023673831025896 29.204400797148406 22.397497012011943 50-51 21.253144198064703 27.063885886044243 29.04115801407968 22.641811901811373 52-53 20.851179466570045 27.38796689360988 28.97340497629569 22.787448663524376 54-55 20.762444644358585 27.317375332707392 29.026127032091143 22.894052990842876 56-57 21.256678040332872 27.0201970257586 28.71075419300107 23.01237074090746 58-59 20.955257053292776 27.031962374527147 28.94877130320618 23.064009268973898 60-61 20.956683542751907 27.063027267807723 28.77193028711316 23.208358902327213 62-63 21.117498371253056 26.63233928136864 28.71281137673477 23.537350970643534 64-65 20.718754082601603 26.666660514613127 28.82961707553434 23.78496832725093 66-67 21.082062373230833 26.51611801751977 28.549302617154982 23.852516992094415 68-69 20.746064972078035 26.555774250618562 28.663108978918476 24.03505179838492 70-71 20.679284747472696 26.288913564731324 28.69088356538792 24.34091812240806 72-73 20.865045337898568 26.183682997698 28.795284663408083 24.15598700099535 74-75 20.89089542635047 26.24457050411903 28.679840050600813 24.184694018929694 76-77 20.824682120234538 26.105719614617374 28.68088218448343 24.388716080664654 78-79 20.78078154873449 26.157527753142297 28.73689325097994 24.324797447143272 80-81 20.747590346675285 26.209519551022375 28.80476607663698 24.238124025665357 82-83 20.97179226375309 25.607421245757315 29.226269851690034 24.194516638799556 84-85 21.077223547592524 25.686651879989338 28.788993798110578 24.447130774307563 86-87 20.816838019333822 25.77871898206524 29.037640292002976 24.36680270659796 88-89 21.38489875553361 25.208449142073906 28.899178409898152 24.507473692494326 90-91 21.570941024954884 25.122968485741666 28.837530269216423 24.468560220087028 92-93 21.736595453720373 24.990630800549486 28.752665921173232 24.52010782455691 94-95 21.2944320594081 24.698913109247886 29.031302965145116 24.975351866198896 96-97 21.500451112436416 24.455712148608196 28.92222621219 25.121610526765387 98-99 21.57096866051795 24.5811119372683 28.78959622006718 25.05832318214657 100 22.05259362479945 24.288277504076948 28.598597556072047 25.060531315051556 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2342.0 1 1587.0 2 1118.0 3 1544.0 4 1287.5 5 1079.0 6 1613.0 7 2561.5 8 3944.0 9 4752.5 10 3971.5 11 3111.0 12 3156.0 13 3481.5 14 3967.0 15 4353.5 16 4787.5 17 5352.0 18 5841.5 19 6397.5 20 7130.5 21 8031.0 22 9154.0 23 10719.5 24 12720.0 25 15094.5 26 18197.5 27 21898.5 28 26450.5 29 33283.0 30 41571.5 31 49150.0 32 58589.5 33 68102.0 34 76502.5 35 85112.0 36 93848.5 37 106541.0 38 113917.5 39 113167.0 40 112310.5 41 112874.0 42 115579.5 43 121755.0 44 130385.5 45 140642.5 46 152139.0 47 161712.5 48 166621.5 49 164470.0 50 158724.5 51 156437.5 52 151133.5 53 142627.0 54 133517.0 55 118143.0 56 103479.0 57 86765.0 58 70686.0 59 61070.0 60 49729.0 61 38262.5 62 29667.5 63 22871.5 64 16599.5 65 10966.5 66 7546.0 67 5603.0 68 4293.0 69 3179.5 70 2437.5 71 1909.5 72 1413.5 73 1052.5 74 786.0 75 570.5 76 410.0 77 269.0 78 193.0 79 156.0 80 111.0 81 77.5 82 56.0 83 50.5 84 41.5 85 28.0 86 20.0 87 14.0 88 15.0 89 15.0 90 9.0 91 5.0 92 8.0 93 8.0 94 4.5 95 2.5 96 1.0 97 1.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2030726601569543 2 0.13453827329193854 3 0.022800863322399462 4 0.021720128760297292 5 0.026148504526959843 6 0.05118991511225405 7 0.05738436930966894 8 0.05037277434578656 9 0.03872192857873388 10-11 0.04112063211900943 12-13 0.03938091306782057 14-15 0.019242347081331337 16-17 0.017449909271015543 18-19 0.01632963563956817 20-21 0.01928188615067654 22-23 0.021825566278551162 24-25 0.01937414397914868 26-27 0.01730493268341647 28-29 0.0171994951651626 30-31 0.015578393322009342 32-33 0.022959019599780268 34-35 0.02331487122388708 36-37 0.022260496041348377 38-39 0.020533956679941248 40-41 0.021258839617936606 42-43 0.02692610622408214 44-45 0.03645502193627567 46-47 0.04112063211900943 48-49 0.038181561297682805 50-51 0.02678112963648307 52-53 0.041595100951151855 54-55 0.04039574918101408 56-57 0.042781273031507895 58-59 0.04508771874331131 60-61 0.023024918048688937 62-63 0.024329707337080585 64-65 0.024896433997695134 66-67 0.030141950530825187 68-69 0.03351595111494904 70-71 0.03777299091444905 72-73 0.04163464002049705 74-75 0.023499386880831353 76-77 0.017252213924289536 78-79 0.016659127884111516 80-81 0.01958501901565642 82-83 0.018820597008315856 84-85 0.011505869179453601 86-87 0.012309830256139364 88-89 0.01698862012865486 90-91 0.01768714368708675 92-93 0.01851746414333598 94-95 0.021944183486586766 96-97 0.0229326602202168 98-99 0.02783550481902177 100 0.027703707921204437 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3793716.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.52357346158072 #Duplication Level Percentage of deduplicated Percentage of total 1 78.16357992086593 51.99720650885862 2 13.75012409417386 18.294147805692518 3 3.986398448224508 7.955684100527834 4 1.5976760481627243 4.251324798310439 5 0.7840661658712729 2.6079441592038783 6 0.47742958813767306 1.9056193367525236 7 0.2784441055019158 1.2966167835110582 8 0.21026846926584028 1.1190247969488203 9 0.13310234237021237 0.7968999105515964 >10 0.5957229960812 6.476604212276257 >50 0.01673417109740113 0.7349066703447972 >100 0.005345186197288242 0.6292278239613334 >500 5.76401310768732E-4 0.2637496192850264 >1k 3.9904705458591384E-4 0.5476152281235928 >5k 4.4338561620657086E-5 0.20744252393586274 >10k+ 8.867712324131417E-5 0.9159857217159417 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16656 0.4390418260091161 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14399 0.37954870633436977 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7033 0.18538551646986753 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.6359379563467587E-5 2 0.0 0.0 0.0 0.0 2.6359379563467587E-5 3 0.0 0.0 0.0 0.0 2.6359379563467587E-5 4 0.0 0.0 0.0 0.0 2.6359379563467587E-5 5 0.0 0.0 0.0 0.0 2.6359379563467587E-5 6 0.0 0.0 0.0 0.0 5.2718759126935174E-5 7 0.0 0.0 0.0 2.6359379563467587E-5 5.2718759126935174E-5 8 0.0 0.0 0.0 2.6359379563467587E-5 5.2718759126935174E-5 9 0.0 0.0 0.0 5.2718759126935174E-5 5.2718759126935174E-5 10-11 0.0 0.0 0.0 5.2718759126935174E-5 5.2718759126935174E-5 12-13 0.0 0.0 0.0 5.2718759126935174E-5 1.1861720803560414E-4 14-15 0.0 0.0 0.0 7.907813869040277E-5 1.7133596716253932E-4 16-17 0.0 0.0 0.0 1.0543751825387035E-4 2.108750365077407E-4 18-19 0.0 0.0 0.0 1.1861720803560414E-4 2.108750365077407E-4 20-21 0.0 0.0 0.0 4.4810945257894896E-4 2.635937956346759E-4 22-23 2.6359379563467587E-5 0.0 0.0 0.0019505940876966014 2.8995317519814345E-4 24-25 2.6359379563467587E-5 0.0 0.0 0.004705149252078964 2.8995317519814345E-4 26-27 2.6359379563467587E-5 0.0 0.0 0.011624486387489205 3.2949224454334485E-4 28-29 2.6359379563467587E-5 0.0 0.0 0.03731170177208837 3.4267193432507864E-4 30-31 2.6359379563467587E-5 0.0 0.0 0.12083139591893541 3.4267193432507864E-4 32-33 2.6359379563467587E-5 0.0 0.0 0.30395000574634473 3.4267193432507864E-4 34-35 2.6359379563467587E-5 0.0 0.0 0.6010729321857513 3.690313138885462E-4 36-37 2.6359379563467587E-5 0.0 0.0 1.0674362551124017 3.690313138885462E-4 38-39 2.6359379563467587E-5 0.0 0.0 1.6535634190856667 3.953906934520138E-4 40-41 2.6359379563467587E-5 0.0 0.0 2.2256673931311672 3.953906934520138E-4 42-43 2.6359379563467587E-5 0.0 0.0 2.7816130675042623 3.953906934520138E-4 44-45 2.6359379563467587E-5 0.0 0.0 3.4657180453149365 3.953906934520138E-4 46-47 2.6359379563467587E-5 0.0 0.0 4.274819201015575 4.217500730154814E-4 48-49 2.6359379563467587E-5 0.0 0.0 5.090615639125333 4.4810945257894896E-4 50-51 2.6359379563467587E-5 0.0 0.0 5.862022882050212 5.008282117058842E-4 52-53 2.6359379563467587E-5 0.0 0.0 6.584915686888529 5.271875912693518E-4 54-55 2.6359379563467587E-5 0.0 0.0 7.3738914562924585 5.535469708328193E-4 56-57 2.6359379563467587E-5 0.0 0.0 8.288232434900241 5.799063503962869E-4 58-59 2.6359379563467587E-5 0.0 0.0 9.275180324515594 6.062657299597545E-4 60-61 2.6359379563467587E-5 0.0 0.0 10.230233364859151 6.062657299597545E-4 62-63 2.6359379563467587E-5 0.0 0.0 11.1399087332842 6.194454197414882E-4 64-65 2.6359379563467587E-5 0.0 0.0 12.05439468847958 6.326251095232221E-4 66-67 2.6359379563467587E-5 0.0 0.0 13.065263715048781 6.326251095232221E-4 68-69 2.6359379563467587E-5 0.0 0.0 14.143309620435478 6.326251095232221E-4 70-71 2.6359379563467587E-5 0.0 0.0 15.236090418998154 6.458047993049559E-4 72-73 2.6359379563467587E-5 0.0 0.0 16.282215115733493 6.589844890866897E-4 74-75 2.6359379563467587E-5 0.0 0.0 17.279140030513616 6.589844890866897E-4 76-77 2.6359379563467587E-5 0.0 0.0 18.333172541117996 6.589844890866897E-4 78-79 2.6359379563467587E-5 0.0 0.0 19.428628816706365 6.721641788684235E-4 80-81 2.6359379563467587E-5 0.0 0.0 20.542220345434398 7.117032482136248E-4 82-83 2.6359379563467587E-5 0.0 0.0 21.630546408850847 7.6442200734056E-4 84-85 2.6359379563467587E-5 0.0 0.0 22.662977407902964 7.6442200734056E-4 86-87 2.6359379563467587E-5 0.0 0.0 23.694222234874722 8.30320456249229E-4 88 2.6359379563467587E-5 0.0 0.0 24.48825900515484 8.962189051578979E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7025 0.0 54.646122 1 GTATCAA 12705 0.0 40.411026 1 CAACGCA 14570 0.0 34.232914 5 ATCAACG 14580 0.0 34.209435 3 TATCAAC 15425 0.0 33.100445 2 AACGCAG 15830 0.0 31.716413 6 TCAACGC 16135 0.0 31.057789 4 GTACTAG 1585 0.0 30.015125 1 GTGGTAT 2630 0.0 29.01398 1 ACGCAGA 17210 0.0 28.627266 7 CGCAGAG 17600 0.0 27.885336 8 GTACATG 12470 0.0 25.798965 1 GCAGAGT 18810 0.0 25.391768 9 TAGGCAT 2265 0.0 25.11338 5 TACATGG 12635 0.0 24.87886 2 TCTAACG 880 0.0 24.063519 2 CTAACGC 865 0.0 23.91248 3 CTGTGCG 2050 0.0 23.848854 9 ACATGGG 12620 0.0 23.72838 3 GAGTACT 12110 0.0 23.695381 12-13 >>END_MODULE