FastQCFastQC Report
Mon 6 Jun 2016
ERR1211443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211443_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3793716
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145990.38482058224706334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134520.354586373887766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74740.19701000285735673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71300.056.5091671
GTATCAA127500.040.413641
ATCAACG155500.032.6079863
CAACGCA157500.032.1001785
TATCAAC161150.031.696772
AACGCAG170450.030.0189236
TCAACGC170450.029.7763274
GTGGTAT25250.027.5567461
GTACTAG17950.027.2393361
ACGCAGA191000.026.3217417
CGCAGAG193800.025.9172068
GCAGAGT205550.024.0699469
GTACATG121800.023.6228221
CTGTGCG20250.023.2027389
TACATGG125700.022.9161112
TAGGCAT23350.022.7387855
GAGTACT132650.022.45523612-13
TGGTATC30650.022.2307072
CTAGGCA24550.022.2050064
ACATGGG124250.022.1632863
Produced by FastQC (version 0.11.5)