FastQCFastQC Report
Mon 6 Jun 2016
ERR1211442_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211442_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2935718
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152820.5205540859169716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127510.4343400830733742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73280.24961525596123332No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53680.18285135016374188No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47360.16132339686577524No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40360.1374791447952426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54000.059.1604961
TACCTGG25550.048.0741582
GTATCAA100850.043.40461
ACCTGGG30050.039.731993
ATCAACG123000.035.4262923
CAACGCA123650.035.202055
TATCAAC126800.034.330852
TCAACGC128050.034.065874
AACGCAG131650.033.6347736
GTACATG95200.030.1917841
ACGCAGA147350.029.54067
GTGGTAT19400.029.3886431
TACATGG97300.029.1169382
CCTGGGG29000.029.01394
CGCAGAG150950.028.7426688
CATGGGG74950.026.9052334
ATGGGGG58150.026.8373375
ACATGGG101250.026.7410553
GCAGAGT158700.026.3310689
TGGGGGG63550.026.1846146
Produced by FastQC (version 0.11.5)