##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211442_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2935718 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.407888632355014 31.0 31.0 34.0 30.0 34.0 2 31.442866446981625 31.0 31.0 34.0 28.0 34.0 3 31.508842811196445 31.0 31.0 34.0 28.0 34.0 4 35.14135417638887 37.0 35.0 37.0 32.0 37.0 5 35.1975516721974 37.0 35.0 37.0 32.0 37.0 6 35.271386079998145 37.0 35.0 37.0 32.0 37.0 7 35.210699733421265 37.0 35.0 37.0 32.0 37.0 8 35.213753500847154 37.0 35.0 37.0 32.0 37.0 9 36.78931866071605 39.0 37.0 39.0 32.0 39.0 10-11 36.71763040591773 39.0 37.0 39.0 32.0 39.0 12-13 36.629608838451105 39.0 37.0 39.0 32.0 39.0 14-15 37.81046459503263 40.0 37.0 41.0 32.0 41.0 16-17 37.795607071251396 40.0 37.0 41.0 32.0 41.0 18-19 37.80556596376083 40.0 37.0 41.0 32.0 41.0 20-21 37.78906710385671 40.0 37.0 41.0 32.0 41.0 22-23 37.6561216029605 40.0 37.0 41.0 32.0 41.0 24-25 37.55817231082822 40.0 37.0 41.0 32.0 41.0 26-27 37.41238174102554 40.0 36.5 41.0 31.5 41.0 28-29 37.27100968144761 40.0 36.0 41.0 31.0 41.0 30-31 37.091184166871614 39.0 36.0 41.0 31.0 41.0 32-33 36.85557349173183 39.0 35.5 41.0 30.0 41.0 34-35 36.70958109736698 39.0 35.0 41.0 30.0 41.0 36-37 36.556998492362005 39.0 35.0 41.0 30.0 41.0 38-39 36.3967480868394 39.0 35.0 40.5 30.0 41.0 40-41 36.21723595386206 39.0 35.0 40.0 29.0 41.0 42-43 36.005700820037895 38.5 35.0 40.0 28.0 41.0 44-45 35.84929819553513 38.0 35.0 40.0 27.5 41.0 46-47 35.59466270261653 38.0 34.5 40.0 26.5 41.0 48-49 35.32686109496893 38.0 34.0 40.0 26.0 41.0 50-51 34.63760603027948 37.5 33.5 39.5 25.0 40.5 52-53 34.9244632829175 38.0 33.5 39.5 25.5 40.5 54-55 35.32573632753555 38.0 34.0 40.0 26.0 41.0 56-57 35.24154516884796 38.0 34.0 40.0 26.0 41.0 58-59 35.029409841135966 38.0 34.0 40.0 26.0 41.0 60-61 34.80078553185285 37.5 34.0 40.0 25.0 41.0 62-63 34.505021088537795 37.0 33.5 40.0 25.0 41.0 64-65 34.28520024743521 37.0 33.0 40.0 24.5 41.0 66-67 34.063909578508564 36.0 33.0 39.5 23.5 41.0 68-69 33.70791949362984 36.0 33.0 39.0 23.0 41.0 70-71 33.3131540563501 35.0 32.5 39.0 22.5 41.0 72-73 32.91075164576434 35.0 32.0 38.0 21.5 40.0 74-75 32.521277758967315 35.0 32.0 37.0 20.5 39.5 76-77 32.13120793618461 35.0 31.0 37.0 20.0 39.0 78-79 31.72653044331915 35.0 31.0 36.0 19.5 39.0 80-81 31.322348059316326 35.0 31.0 36.0 18.0 37.5 82-83 30.95030534267937 34.5 30.0 35.0 18.0 37.0 84-85 30.608940129808108 34.0 30.0 35.0 16.0 37.0 86-87 30.27236948507997 34.0 29.0 35.0 14.5 36.0 88-89 29.948015613216256 34.0 29.0 35.0 11.5 36.0 90-91 29.617482333112378 34.0 29.0 35.0 10.0 35.5 92-93 29.30501703501494 34.0 27.5 35.0 10.0 35.0 94-95 29.00214189510028 34.0 27.0 35.0 10.0 35.0 96-97 28.684192419026623 34.0 25.5 35.0 9.0 35.0 98-99 28.342662680816076 33.5 25.0 35.0 8.0 35.0 100 27.068284828447418 32.0 23.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3457708952631684 1101 2 -0.2759895362013651 1101 3 -0.3684789288182593 1101 4 -0.31798394598705215 1101 5 -0.2772092819852858 1101 6 -0.25866882677977543 1101 7 -0.26192290585337474 1101 8 -0.3686701853480443 1101 9 -0.3585473265643415 1101 10-11 -0.4389624779578867 1101 12-13 -0.45078093870846914 1101 14-15 -0.7041502350754811 1101 16-17 -0.70724289769786 1101 18-19 -0.6473311246370059 1101 20-21 -0.5711805051651453 1101 22-23 -0.6946445654700923 1101 24-25 -0.715715286746267 1101 26-27 -0.8004253716732066 1101 28-29 -0.8904663468901575 1101 30-31 -0.8191170086208643 1101 32-33 -0.6786241586151576 1101 34-35 -0.7375105057699045 1101 36-37 -0.8052727069769645 1101 38-39 -0.9054459397627568 1101 40-41 -1.0503618148308647 1101 42-43 -0.8666180689551908 1101 44-45 -0.7997034636480436 1101 46-47 -0.8128960284193667 1101 48-49 -0.946194480412089 1101 50-51 -0.7497586462935217 1101 52-53 -0.5345771859772412 1101 54-55 -0.684478021129209 1101 56-57 -0.7369368770827336 1101 58-59 -0.730528988065835 1101 60-61 -0.7576018104802671 1101 62-63 -0.7206835449611546 1101 64-65 -0.7208921415788723 1101 66-67 -0.6986216385865092 1101 68-69 -0.7276812139377995 1101 70-71 -0.6931325314267198 1101 72-73 -1.0006441305798717 1101 74-75 -1.0827383937448758 1101 76-77 -0.8337685994442054 1101 78-79 -0.8582919448413548 1101 80-81 -1.1428434939729932 1101 82-83 -1.085715822172947 1101 84-85 -1.0582455529015533 1101 86-87 -1.2498327096942496 1101 88-89 -1.353899368475492 1101 90-91 -1.3577403647321233 1101 92-93 -1.4694458572654767 1101 94-95 -1.6653995886840036 1101 96-97 -1.8329456992439042 1101 98-99 -1.8055667840880822 1101 100 -1.8611905316634427 1102 1 -0.39057895911532725 1102 2 -0.3528672857889923 1102 3 -0.4628002697415532 1102 4 -0.36264190549086806 1102 5 -0.32169148112560464 1102 6 -0.35069195517763774 1102 7 -0.3721159906909861 1102 8 -0.3293783313494316 1102 9 -0.6015674327169407 1102 10-11 -0.44902888205578506 1102 12-13 -0.5692084382249192 1102 14-15 -0.7465368042515124 1102 16-17 -0.7797551263709224 1102 18-19 -0.7436572998865785 1102 20-21 -0.8003262881409157 1102 22-23 -0.771293243493929 1102 24-25 -0.7663807521338839 1102 26-27 -0.8085631598015013 1102 28-29 -0.8957977168164035 1102 30-31 -0.921620202711452 1102 32-33 -0.8063814513791172 1102 34-35 -0.7923801423924388 1102 36-37 -0.8184055046771164 1102 38-39 -0.9656272045076761 1102 40-41 -1.154990126643412 1102 42-43 -1.0947156204550623 1102 44-45 -1.1296984607250877 1102 46-47 -1.2249661173207897 1102 48-49 -1.289604741252134 1102 50-51 -1.1794208482026889 1102 52-53 -0.7889728193901107 1102 54-55 -0.8644996987960312 1102 56-57 -0.8911301944904579 1102 58-59 -0.9265935885791876 1102 60-61 -0.8919125491386026 1102 62-63 -0.8865904992573022 1102 64-65 -1.0119997000908256 1102 66-67 -1.3520639344600056 1102 68-69 -1.0271203134716416 1102 70-71 -0.947679768565429 1102 72-73 -0.8611258872372325 1102 74-75 -1.1073484264796178 1102 76-77 -1.1324056986085367 1102 78-79 -0.9632507027970085 1102 80-81 -1.1757504154494285 1102 82-83 -1.3500332530528958 1102 84-85 -1.2801830785055088 1102 86-87 -1.4335936682696122 1102 88-89 -1.6130187907286846 1102 90-91 -1.5646649565390973 1102 92-93 -1.7856000172633841 1102 94-95 -1.7220850547680016 1102 96-97 -1.8313716860550429 1102 98-99 -1.7675429869678503 1102 100 -1.7320129952039984 1103 1 -0.34574755258109136 1103 2 -0.46505008828580685 1103 3 -0.5456502192534103 1103 4 -0.39143586348001236 1103 5 -0.2471847571630903 1103 6 -0.26226810162268066 1103 7 -0.4453320278734054 1103 8 -0.3576861195630343 1103 9 -0.5655725735090797 1103 10-11 -0.5680099114550075 1103 12-13 -0.6997956691079921 1103 14-15 -0.8491860719281377 1103 16-17 -0.7747316769539552 1103 18-19 -0.7802124269319108 1103 20-21 -0.9107955209789793 1103 22-23 -0.7337494769871498 1103 24-25 -0.7895035055945812 1103 26-27 -0.8357067518659775 1103 28-29 -0.8490758533308096 1103 30-31 -0.9148440139531715 1103 32-33 -0.8592684233098353 1103 34-35 -0.9349388258120825 1103 36-37 -0.9501019610932389 1103 38-39 -0.8827680239241289 1103 40-41 -0.9745161052908671 1103 42-43 -0.9539357063356277 1103 44-45 -0.8246532060198604 1103 46-47 -0.9761104791402104 1103 48-49 -0.9855349862846907 1103 50-51 -0.9027469180963763 1103 52-53 -0.7206325344776516 1103 54-55 -0.7983344539638892 1103 56-57 -0.7969086457760923 1103 58-59 -0.7240544783580347 1103 60-61 -0.7835678765563969 1103 62-63 -0.7199273254285643 1103 64-65 -0.7195557730299313 1103 66-67 -0.7079767186294035 1103 68-69 -0.8073831345570142 1103 70-71 -0.8505932611156268 1103 72-73 -0.9105480470984375 1103 74-75 -1.0093991880596676 1103 76-77 -0.9070874636493187 1103 78-79 -0.9831291087921841 1103 80-81 -0.9637929508971581 1103 82-83 -0.9522657087358475 1103 84-85 -0.9736304979053614 1103 86-87 -1.2141372469947704 1103 88-89 -1.2144651083876603 1103 90-91 -1.2661004962981508 1103 92-93 -1.533372459869824 1103 94-95 -1.4376148609005277 1103 96-97 -1.6845406797002411 1103 98-99 -1.7545611063141315 1103 100 -1.7963537311915232 1104 1 -0.5156781931359689 1104 2 -0.42237842965907646 1104 3 -0.526162375156165 1104 4 -0.277271776792702 1104 5 -0.3158272080234852 1104 6 -0.36157030720504224 1104 7 -0.281698336942938 1104 8 -0.40288975793392723 1104 9 -0.4501094972136883 1104 10-11 -0.49845846786655557 1104 12-13 -0.5577768052589036 1104 14-15 -0.6431642201029533 1104 16-17 -0.6360697862490738 1104 18-19 -0.7396602513493065 1104 20-21 -0.874615810416671 1104 22-23 -0.7825335867142726 1104 24-25 -0.8369541259896209 1104 26-27 -0.7952520755033845 1104 28-29 -0.8366844524620234 1104 30-31 -0.8341986591160122 1104 32-33 -0.9560521490400262 1104 34-35 -0.8906996797252518 1104 36-37 -0.9555514174975883 1104 38-39 -1.0116814926163826 1104 40-41 -0.97992806199521 1104 42-43 -0.9769245224250938 1104 44-45 -0.8513964920735759 1104 46-47 -0.9148494692282938 1104 48-49 -0.92825044456103 1104 50-51 -0.7975017600576422 1104 52-53 -0.7396823820788683 1104 54-55 -0.7642487266497113 1104 56-57 -0.6423393823301993 1104 58-59 -0.7477932884475464 1104 60-61 -0.7888583342327351 1104 62-63 -0.9097868065527237 1104 64-65 -0.9587273596572388 1104 66-67 -0.8129128791401214 1104 68-69 -0.7898782745958854 1104 70-71 -0.8842379919577823 1104 72-73 -0.8449655717582374 1104 74-75 -0.8554404197298133 1104 76-77 -0.9702354584653605 1104 78-79 -0.8391062609749653 1104 80-81 -1.0939034100134855 1104 82-83 -1.1427191851080387 1104 84-85 -1.0739541953157854 1104 86-87 -1.2162537300629062 1104 88-89 -1.1078939609568401 1104 90-91 -1.290743799151727 1104 92-93 -1.363575318246955 1104 94-95 -1.4564067105657301 1104 96-97 -1.4502080543612443 1104 98-99 -1.5131380376986847 1104 100 -1.3360014140100596 1105 1 -0.426382921057467 1105 2 -0.4037279483544509 1105 3 -0.4704884612254432 1105 4 -0.3065315122063481 1105 5 -0.36282670660376937 1105 6 -0.406214882251831 1105 7 -0.39482664208598806 1105 8 -0.2828166439425672 1105 9 -0.44584742961531276 1105 10-11 -0.47544213674297 1105 12-13 -0.6157245993036682 1105 14-15 -0.6567330895180987 1105 16-17 -0.5635818407289079 1105 18-19 -0.6092850757066444 1105 20-21 -0.6657616710802898 1105 22-23 -0.6620642199607332 1105 24-25 -0.7205951619460791 1105 26-27 -0.7404187684071317 1105 28-29 -0.7449668421351063 1105 30-31 -0.7479675862527273 1105 32-33 -0.7693860508433943 1105 34-35 -0.7782390353426152 1105 36-37 -0.7837202006218931 1105 38-39 -0.8572931226385876 1105 40-41 -0.8171462320464542 1105 42-43 -0.7782216544209248 1105 44-45 -0.7102098910579002 1105 46-47 -0.9899235889743139 1105 48-49 -1.1207806026248477 1105 50-51 -1.0808928149631 1105 52-53 -0.8907452752706462 1105 54-55 -0.7071635494328206 1105 56-57 -0.7439130968591314 1105 58-59 -0.7140735412563757 1105 60-61 -0.5703803617175325 1105 62-63 -0.6058505160091414 1105 64-65 -0.6676631983400227 1105 66-67 -0.6396098350559996 1105 68-69 -0.7220952202501536 1105 70-71 -0.6611871153478432 1105 72-73 -0.7266606910786422 1105 74-75 -0.7280384152170356 1105 76-77 -0.7070777823708383 1105 78-79 -0.6986860012861982 1105 80-81 -0.9174763315996053 1105 82-83 -0.8175486440994852 1105 84-85 -0.8920090220636112 1105 86-87 -1.013125322886772 1105 88-89 -0.9562815467375074 1105 90-91 -1.0855428085562622 1105 92-93 -1.0176844091030404 1105 94-95 -1.1452040612038772 1105 96-97 -1.092826471041029 1105 98-99 -1.0804158825267685 1105 100 -1.3144561084064037 1106 1 -0.4937304133776763 1106 2 -0.5537136856387335 1106 3 -0.5485347425114284 1106 4 -0.5795540935546413 1106 5 -0.40024366220431773 1106 6 -0.4936183460942658 1106 7 -0.5228627957925056 1106 8 -0.6033867040141843 1106 9 -0.7162446900525055 1106 10-11 -0.8171595639593079 1106 12-13 -0.7770207051967972 1106 14-15 -0.8009013208782747 1106 16-17 -0.7928029562765317 1106 18-19 -0.879011182503902 1106 20-21 -0.9538690893044688 1106 22-23 -0.8067652531140155 1106 24-25 -0.8509201486120119 1106 26-27 -0.8493571808091716 1106 28-29 -0.9104194608327916 1106 30-31 -0.8037144794687876 1106 32-33 -1.053222434014934 1106 34-35 -0.99981372326247 1106 36-37 -1.0107398116807929 1106 38-39 -1.039977274623773 1106 40-41 -1.0001682737244266 1106 42-43 -1.0054091940639154 1106 44-45 -0.9398631633874714 1106 46-47 -0.9092179945554903 1106 48-49 -0.8389800381437027 1106 50-51 -1.0363069348413987 1106 52-53 -0.8380009114964011 1106 54-55 -0.724197787660124 1106 56-57 -0.8829913259274562 1106 58-59 -0.8916226249732588 1106 60-61 -0.7431134134410726 1106 62-63 -0.9753505920535943 1106 64-65 -0.7811726183906629 1106 66-67 -0.7000202508334326 1106 68-69 -0.7540915028327504 1106 70-71 -0.8046602232952225 1106 72-73 -0.7585414001073261 1106 74-75 -0.8236153613418189 1106 76-77 -0.7265010780463967 1106 78-79 -0.78797312482234 1106 80-81 -0.8268133991712361 1106 82-83 -0.7985279985556524 1106 84-85 -0.8360684082271632 1106 86-87 -0.7822574418917903 1106 88-89 -0.8467530013312583 1106 90-91 -0.9312204347357422 1106 92-93 -0.9201515435307144 1106 94-95 -0.998290519894212 1106 96-97 -1.1841023147665197 1106 98-99 -1.0622117511752194 1106 100 -1.2326457317375414 1107 1 -0.5172284185368383 1107 2 -0.48596172728109366 1107 3 -0.5031903955781871 1107 4 -0.38862073023670973 1107 5 -0.44012622194362194 1107 6 -0.3933799981557726 1107 7 -0.42197373552037476 1107 8 -0.4865756356098956 1107 9 -0.5846081942635024 1107 10-11 -0.609045875180307 1107 12-13 -0.6769494738508115 1107 14-15 -0.8789340768064022 1107 16-17 -0.8627180825675893 1107 18-19 -0.9087571289682188 1107 20-21 -0.8948929376828403 1107 22-23 -0.8166868676983583 1107 24-25 -0.9207727322748838 1107 26-27 -0.8061980598545588 1107 28-29 -0.8661266370546485 1107 30-31 -1.0009569817172945 1107 32-33 -0.9754175888039001 1107 34-35 -1.1064100337316134 1107 36-37 -1.0835958537492445 1107 38-39 -1.071623844483419 1107 40-41 -1.070482260582942 1107 42-43 -1.0613207569046637 1107 44-45 -1.1130917846695283 1107 46-47 -1.0447679103650387 1107 48-49 -1.1523334003614139 1107 50-51 -1.2110670435122444 1107 52-53 -0.9356370826017297 1107 54-55 -0.8698662937077302 1107 56-57 -0.8993020398562663 1107 58-59 -1.0725883073624303 1107 60-61 -0.8441309887265973 1107 62-63 -0.90525906865102 1107 64-65 -0.8974244346487623 1107 66-67 -0.935283761755997 1107 68-69 -0.8478303267004677 1107 70-71 -0.7744118510511839 1107 72-73 -0.833300419325937 1107 74-75 -0.8213175586324724 1107 76-77 -0.802457754969609 1107 78-79 -0.7918196309557572 1107 80-81 -0.9485064913312264 1107 82-83 -0.8994497423947685 1107 84-85 -0.9820547384261502 1107 86-87 -0.9275825014854995 1107 88-89 -1.0142644866460486 1107 90-91 -1.029498441156516 1107 92-93 -1.0188597490831839 1107 94-95 -1.0331157347684936 1107 96-97 -1.1900992113071602 1107 98-99 -1.101485360916481 1107 100 -1.1563839409390013 1108 1 -0.5716690332351355 1108 2 -0.5645005030384773 1108 3 -0.5725819649644954 1108 4 -0.42264387271561077 1108 5 -0.37563638974420854 1108 6 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1.0746852873044759 2115 80-81 1.0934416143625718 2115 82-83 1.0955812606734838 2115 84-85 1.1494551600828942 2115 86-87 0.9962410082825279 2115 88-89 1.1374640658961717 2115 90-91 1.3415041852808471 2115 92-93 1.4566435068919041 2115 94-95 1.5480099841778312 2115 96-97 1.5650620741204015 2115 98-99 1.6236478474486837 2115 100 1.689966840968836 2116 1 0.5018656665131545 2116 2 0.41567258021855835 2116 3 0.40202574219603804 2116 4 0.2218326018008412 2116 5 0.2215788070141116 2116 6 0.2553433003432417 2116 7 0.28354934107564134 2116 8 0.3352054801613136 2116 9 0.43771300020632964 2116 10-11 0.4137632629310275 2116 12-13 0.45563908741395664 2116 14-15 0.6357149631492902 2116 16-17 0.6355762405827079 2116 18-19 0.7131943115284685 2116 20-21 0.7070294792781553 2116 22-23 0.6936108184161967 2116 24-25 0.4425424094666681 2116 26-27 0.5356007919593893 2116 28-29 0.6854941558233989 2116 30-31 0.721079241517927 2116 32-33 0.6091236581249362 2116 34-35 0.843538489226539 2116 36-37 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0.5990534249912365 2201 2 0.6029636256689983 2201 3 0.6740312193248563 2201 4 0.4944528657873235 2201 5 0.4680230174526301 2201 6 0.5045479698040793 2201 7 0.5706388170645482 2201 8 0.5581560747912206 2201 9 0.6477747916722976 2201 10-11 0.6913169394885017 2201 12-13 0.44791819508679964 2201 14-15 0.6894087235318622 2201 16-17 0.8779033947694401 2201 18-19 0.8758357576850955 2201 20-21 0.8222743861739161 2201 22-23 0.8901918701917495 2201 24-25 0.8354419514174438 2201 26-27 0.8507600318339925 2201 28-29 0.8257769438765834 2201 30-31 0.7543093573856154 2201 32-33 0.8214289524511074 2201 34-35 0.8357429640685865 2201 36-37 0.36638967446747017 2201 38-39 0.658406047427718 2201 40-41 0.7572232566751325 2201 42-43 0.7175889562065265 2201 44-45 0.6823481014932469 2201 46-47 0.645346314714125 2201 48-49 0.7334254344885238 2201 50-51 0.7158899653147586 2201 52-53 0.8087983429785481 2201 54-55 0.7940994591702619 2201 56-57 0.8028928734296201 2201 58-59 0.7126883738529912 2201 60-61 0.6339554693610552 2201 62-63 0.6468463011764598 2201 64-65 0.541981334425067 2201 66-67 0.4885629906906459 2201 68-69 -0.05014473588851587 2201 70-71 0.328388478246346 2201 72-73 0.4178898316020039 2201 74-75 0.47170976579823076 2201 76-77 0.41394971619219945 2201 78-79 0.39246289143459023 2201 80-81 0.3147936491941614 2201 82-83 0.3356550821906268 2201 84-85 0.3122720759162263 2201 86-87 0.24554593037852257 2201 88-89 0.2537983390531515 2201 90-91 0.2511647005678661 2201 92-93 0.228169836129279 2201 94-95 0.19101990406601743 2201 96-97 0.0011134008239359616 2201 98-99 0.05966971606757454 2201 100 -0.15849563306375103 2202 1 0.6541303294227774 2202 2 0.6659799667924773 2202 3 0.7172768520325867 2202 4 0.5057562401353195 2202 5 0.576016486907605 2202 6 0.5401021882440489 2202 7 0.6011632495068895 2202 8 0.6651974644315359 2202 9 0.7879849562022585 2202 10-11 0.8248087456200324 2202 12-13 0.8488180872543367 2202 14-15 1.0396523176778274 2202 16-17 1.0367690408086219 2202 18-19 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0.3201553822396548 2202 86-87 0.2930379517088362 2202 88-89 0.28740217285443137 2202 90-91 0.23682445963845566 2202 92-93 0.29033616148187136 2202 94-95 0.21839618030356434 2202 96-97 0.08133146633448618 2202 98-99 0.04326245975106602 2202 100 -0.032722820827409294 2203 1 -0.19080270518334785 2203 2 -0.06369960343751302 2203 3 0.4010149030777832 2203 4 0.45280629562619623 2203 5 0.4921213718661406 2203 6 0.45914917051739934 2203 7 0.5229355303339531 2203 8 0.5488365847770709 2203 9 0.7263106131645571 2203 10-11 0.7484119049965514 2203 12-13 0.7956859757345001 2203 14-15 0.9692583805162442 2203 16-17 0.927658508272394 2203 18-19 0.9820805616807036 2203 20-21 0.9678259717882014 2203 22-23 0.9705792144702698 2203 24-25 0.9557837929765896 2203 26-27 0.9426416517815781 2203 28-29 0.9149729038443439 2203 30-31 0.9535083000978801 2203 32-33 0.907497790386536 2203 34-35 0.8806776927340323 2203 36-37 0.9280634701050516 2203 38-39 0.9194751233480858 2203 40-41 0.968534045960098 2203 42-43 0.9736492425499961 2203 44-45 0.9141002100397628 2203 46-47 0.7684019778036131 2203 48-49 0.7901420039515585 2203 50-51 0.9422596479380516 2203 52-53 0.8999057186076271 2203 54-55 0.8896666724618782 2203 56-57 0.8775741322747237 2203 58-59 0.7750900067469573 2203 60-61 0.7631170325730281 2203 62-63 0.7211106897455792 2203 64-65 0.7140162801327747 2203 66-67 0.7058817096336085 2203 68-69 0.7326011977422411 2203 70-71 0.5683296483706641 2203 72-73 0.5439663167866513 2203 74-75 0.6555721660825427 2203 76-77 0.6095027297160911 2203 78-79 0.5476719056692616 2203 80-81 0.5013230673051297 2203 82-83 0.4679703424192212 2203 84-85 0.4848020302865841 2203 86-87 0.47742139444914145 2203 88-89 0.5270585898575391 2203 90-91 0.5981376115361172 2203 92-93 0.5164977063862395 2203 94-95 0.3591808516902937 2203 96-97 0.46747938674792167 2203 98-99 0.3293962872441121 2203 100 0.3435819754097835 2204 1 0.6191458301123376 2204 2 0.653844290620512 2204 3 0.7514079490665146 2204 4 0.6439855120446936 2204 5 0.6457784255118142 2204 6 0.6828080285658231 2204 7 0.650456594774667 2204 8 0.6897302348484615 2204 9 0.8799841417918017 2204 10-11 0.8442778614084574 2204 12-13 0.8504123341359602 2204 14-15 1.000600856156801 2204 16-17 0.9597131346041223 2204 18-19 0.9940680720756347 2204 20-21 0.9781848125771262 2204 22-23 1.0712748901798719 2204 24-25 1.082210055666252 2204 26-27 1.0589763998712627 2204 28-29 1.0390432441703936 2204 30-31 1.0413383881213605 2204 32-33 1.0164186006418205 2204 34-35 1.0292373086316005 2204 36-37 0.9775956983070841 2204 38-39 0.9517966836072915 2204 40-41 0.9964893438434572 2204 42-43 0.9528125096172957 2204 44-45 0.8752253336021312 2204 46-47 0.8366517463693484 2204 48-49 0.7175424479915407 2204 50-51 0.8287636960763791 2204 52-53 0.8541797995390112 2204 54-55 0.7476921459214694 2204 56-57 0.638214616796084 2204 58-59 0.6974630317104769 2204 60-61 0.7600654344998148 2204 62-63 0.7124752497066638 2204 64-65 0.6356977481320811 2204 66-67 0.5879180958071757 2204 68-69 0.5150282763357552 2204 70-71 0.4856887872782991 2204 72-73 0.45741813923341823 2204 74-75 0.5139887242235375 2204 76-77 0.3808949048843644 2204 78-79 0.414607009932368 2204 80-81 0.5806528493646574 2204 82-83 0.48446081831083276 2204 84-85 0.6078809297578189 2204 86-87 0.485445494993602 2204 88-89 0.46849258009807215 2204 90-91 0.43415957033297303 2204 92-93 0.44020324354909235 2204 94-95 0.47855739131654573 2204 96-97 0.3974754249071175 2204 98-99 0.3593585909172461 2204 100 0.1951663194701574 2205 1 0.727515999241529 2205 2 0.7684154922654116 2205 3 0.843860043974825 2205 4 0.6533312266591338 2205 5 0.6343836385656019 2205 6 0.6400799899374121 2205 7 0.7646033960914735 2205 8 0.688494890299232 2205 9 0.9367628560833481 2205 10-11 0.8861185934479892 2205 12-13 0.8866468807309147 2205 14-15 1.1062508363007382 2205 16-17 1.0372381031498819 2205 18-19 1.0227392184204476 2205 20-21 1.0884904847329722 2205 22-23 1.0668245322138787 2205 24-25 1.1169843459508826 2205 26-27 1.111837529848735 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0.9284461416459351 2205 96-97 0.8420213147003572 2205 98-99 0.7688670510881934 2205 100 0.8171959811004363 2206 1 0.6360286728577123 2206 2 0.6678599559008944 2206 3 0.7217894309804826 2206 4 0.606930476968472 2206 5 0.6140957552282416 2206 6 0.653811091559291 2206 7 0.7194377227826436 2206 8 0.7613768857660119 2206 9 0.9344632083422937 2206 10-11 0.9092211825556831 2206 12-13 0.953144326698542 2206 14-15 1.1269342912905103 2206 16-17 1.1230852119604222 2206 18-19 1.1323229293006207 2206 20-21 1.1631006925365242 2206 22-23 1.1310463782916926 2206 24-25 1.127068987301314 2206 26-27 1.1676951523975632 2206 28-29 1.167642036937778 2206 30-31 1.182139976207118 2206 32-33 1.1230740992091341 2206 34-35 1.1074298730128547 2206 36-37 1.1048844101431712 2206 38-39 1.0950589962122734 2206 40-41 1.1552826501697524 2206 42-43 1.1902609731802443 2206 44-45 1.1860241820603008 2206 46-47 1.1195063934065033 2206 48-49 1.163906153764522 2206 50-51 1.2189764887898704 2206 52-53 1.118060729036273 2206 54-55 1.041866844337875 2206 56-57 1.0360426759720482 2206 58-59 1.0423271282172308 2206 60-61 1.0341802204046573 2206 62-63 1.0510721112611563 2206 64-65 1.0167817424192904 2206 66-67 1.0525084092026091 2206 68-69 0.8703988446512909 2206 70-71 0.8876662594553508 2206 72-73 0.8727881236939012 2206 74-75 0.794732510658342 2206 76-77 0.7631573881546743 2206 78-79 0.8336070009499821 2206 80-81 0.8508306556864618 2206 82-83 0.7404531922655053 2206 84-85 0.784138912852967 2206 86-87 0.8119471252965766 2206 88-89 0.8151536028324919 2206 90-91 0.7877808190642099 2206 92-93 0.8416660250345984 2206 94-95 0.8040554645948461 2206 96-97 0.7864540378471432 2206 98-99 0.6954902367597491 2206 100 0.811263203480614 2207 1 0.6466629503158323 2207 2 0.67898461563788 2207 3 0.7282909876591113 2207 4 0.5831313214085156 2207 5 0.6719959541006233 2207 6 0.682520210652001 2207 7 0.6770854493475866 2207 8 0.6730803544969035 2207 9 0.8679189471746938 2207 10-11 0.869562371070387 2207 12-13 0.90298045655598 2207 14-15 1.1239873481967848 2207 16-17 1.1721562051029721 2207 18-19 1.0350283657013648 2207 20-21 0.9953866196178183 2207 22-23 1.1626495423539467 2207 24-25 1.0747477421689524 2207 26-27 1.1232246731475684 2207 28-29 1.0820289231155726 2207 30-31 1.125568502770662 2207 32-33 1.1084600493620869 2207 34-35 1.1159526556120838 2207 36-37 1.0350086637137963 2207 38-39 1.089608164711656 2207 40-41 1.0687941294135967 2207 42-43 1.1560125081469579 2207 44-45 1.295879944753878 2207 46-47 1.1396272432503949 2207 48-49 1.177314380782562 2207 50-51 1.1512254398191928 2207 52-53 1.1508408544302569 2207 54-55 0.9680574688252293 2207 56-57 0.9968627548016542 2207 58-59 1.0458191062123134 2207 60-61 1.036903903587877 2207 62-63 0.999355641697754 2207 64-65 1.0645644513120445 2207 66-67 1.086428739188655 2207 68-69 1.0191490716395037 2207 70-71 1.0579761126301648 2207 72-73 1.0617825505300047 2207 74-75 1.0648173494243238 2207 76-77 0.9396180299326105 2207 78-79 0.9312888700521285 2207 80-81 0.9649244090583942 2207 82-83 1.0153798323047347 2207 84-85 1.0096713372976893 2207 86-87 0.9950905195665634 2207 88-89 0.8851475147233394 2207 90-91 0.9604068731887274 2207 92-93 0.9254361261024755 2207 94-95 1.101124355848409 2207 96-97 1.0524247535157762 2207 98-99 1.0611790484117236 2207 100 1.3053959856675448 2208 1 0.7301561649555204 2208 2 0.74543988433647 2208 3 0.771039492555996 2208 4 0.7310124887088705 2208 5 0.6985893308498135 2208 6 0.7128614945368099 2208 7 0.7282614081454568 2208 8 0.7879259375031253 2208 9 0.9435667201990015 2208 10-11 0.9981747494882711 2208 12-13 1.0104196444966007 2208 14-15 1.2756080231770497 2208 16-17 1.2499013047205167 2208 18-19 1.2710644455633968 2208 20-21 1.2416636483663552 2208 22-23 1.3039552482442005 2208 24-25 1.2158515111447201 2208 26-27 1.2675327469843936 2208 28-29 1.2779254040236125 2208 30-31 1.3461522964408346 2208 32-33 1.3496225095346617 2208 34-35 1.3677256407226537 2208 36-37 1.34794412299604 2208 38-39 1.4222518968978122 2208 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2310 42-43 1.203232345075719 2310 44-45 1.2493234648395841 2310 46-47 1.2138836241788553 2310 48-49 1.2769716060588863 2310 50-51 1.19057757609108 2310 52-53 1.0449734581859218 2310 54-55 1.0705860773907503 2310 56-57 1.0559724551382317 2310 58-59 1.0113524114283265 2310 60-61 1.0779469617396416 2310 62-63 0.938974535845027 2310 64-65 0.9996093918515143 2310 66-67 1.0333387794180382 2310 68-69 0.987530290697805 2310 70-71 0.9758871489304966 2310 72-73 0.8696135111482661 2310 74-75 0.8094889221257731 2310 76-77 0.956543211406661 2310 78-79 0.9763648765462563 2310 80-81 1.1200599554685304 2310 82-83 1.1086522463168258 2310 84-85 1.033148981268944 2310 86-87 1.1601484649407325 2310 88-89 1.2180840533388633 2310 90-91 1.330303303900017 2310 92-93 1.2175353093657222 2310 94-95 1.2812361394435428 2310 96-97 1.3175238049359557 2310 98-99 1.2347406129126668 2310 100 1.419690293912499 2311 1 0.6368320449953835 2311 2 0.6294626495604163 2311 3 0.7127523383099863 2311 4 0.6070625439217778 2311 5 0.5824220492101233 2311 6 0.5426478475223462 2311 7 0.6250309481276872 2311 8 0.5462541647648393 2311 9 0.8094991786546544 2311 10-11 0.6655367677219886 2311 12-13 0.8207744210863268 2311 14-15 1.012244479855113 2311 16-17 1.0207799474256731 2311 18-19 1.0216537760059055 2311 20-21 1.083609044821955 2311 22-23 1.1140658592221442 2311 24-25 1.1724268127957203 2311 26-27 1.0991365809147382 2311 28-29 1.1374807717954312 2311 30-31 1.198512059025333 2311 32-33 1.3035767131221547 2311 34-35 1.2298569935735273 2311 36-37 1.232662352008397 2311 38-39 1.238285607070452 2311 40-41 1.267180279293683 2311 42-43 1.3359837760376223 2311 44-45 1.3985810911028835 2311 46-47 1.4482706180300298 2311 48-49 1.4554753876496136 2311 50-51 1.404660515439545 2311 52-53 1.2745613345189426 2311 54-55 1.2580619236835275 2311 56-57 1.1809958618235825 2311 58-59 1.1365106970629242 2311 60-61 1.1087805385467746 2311 62-63 1.1179781580710824 2311 64-65 1.1249129317391606 2311 66-67 1.1346150247865054 2311 68-69 1.1940447364407731 2311 70-71 1.194851461205559 2311 72-73 1.0728779967324087 2311 74-75 1.098367094478661 2311 76-77 1.0957169554138844 2311 78-79 1.1837588990432408 2311 80-81 1.1310395999525262 2311 82-83 1.1529881604723897 2311 84-85 1.2285342951509328 2311 86-87 1.2389885723790783 2311 88-89 1.3195299556748843 2311 90-91 1.4017936291367405 2311 92-93 1.500594679344072 2311 94-95 1.3944185854255622 2311 96-97 1.50158161114852 2311 98-99 1.5149490154146896 2311 100 1.6913341736452985 2312 1 0.6331047107054246 2312 2 0.6512368862066609 2312 3 0.7286836015756073 2312 4 0.5365872110898877 2312 5 0.5774868607974852 2312 6 0.49442796271635814 2312 7 0.5715469314763197 2312 8 0.6965794789720192 2312 9 0.8508067846340239 2312 10-11 0.8367590430310514 2312 12-13 0.913270299323024 2312 14-15 1.1393325408197228 2312 16-17 1.1309187672972385 2312 18-19 1.0656528316096257 2312 20-21 1.0403468416507309 2312 22-23 1.0524079492253477 2312 24-25 1.075674012509218 2312 26-27 1.1454328565316914 2312 28-29 1.231142030251327 2312 30-31 1.109131412289365 2312 32-33 1.1517036738012862 2312 34-35 1.1694447055741364 2312 36-37 1.1317878282943923 2312 38-39 1.3034140537379884 2312 40-41 1.3186441732851932 2312 42-43 1.2453313449749572 2312 44-45 1.241535089273114 2312 46-47 1.1054523369429248 2312 48-49 1.2318504852598267 2312 50-51 1.1786955203170635 2312 52-53 1.0367448042340541 2312 54-55 1.0333168668083772 2312 56-57 1.064455407125486 2312 58-59 1.0790669959228723 2312 60-61 0.9227512622622029 2312 62-63 0.923945163226179 2312 64-65 0.9667055777317373 2312 66-67 0.9272041860844453 2312 68-69 0.9103336578907744 2312 70-71 0.9275659384654702 2312 72-73 1.038695318268232 2312 74-75 1.0565933523642528 2312 76-77 0.9878831324674522 2312 78-79 1.0312813743186382 2312 80-81 0.9532303600240191 2312 82-83 1.0135466942971725 2312 84-85 1.135117909716012 2312 86-87 1.245659208594283 2312 88-89 1.2769774056852938 2312 90-91 1.3566565520910743 2312 92-93 1.3939872953456884 2312 94-95 1.4617958757261178 2312 96-97 1.5876645680660246 2312 98-99 1.5955121196707758 2312 100 1.7336674546007949 2313 1 0.7464174952036977 2313 2 0.7488172081663862 2313 3 0.812280168014123 2313 4 0.6608516204372847 2313 5 0.7006196103735434 2313 6 0.6195878497832581 2313 7 0.7176964166199014 2313 8 0.7162788873525017 2313 9 0.8953170310170648 2313 10-11 0.7728252618067586 2313 12-13 0.8970803028995746 2313 14-15 1.129305031353951 2313 16-17 1.139903008242591 2313 18-19 1.14504316249581 2313 20-21 1.2229817726457597 2313 22-23 1.2179681452013753 2313 24-25 1.2058577640337376 2313 26-27 1.202355760006867 2313 28-29 1.269384050610192 2313 30-31 1.225269058433895 2313 32-33 1.320985821978546 2313 34-35 1.3140822951514153 2313 36-37 1.387426733788061 2313 38-39 1.3554270926866252 2313 40-41 1.2636362168924933 2313 42-43 1.3027514738224184 2313 44-45 1.2032031828402268 2313 46-47 1.301312282717035 2313 48-49 1.487208442683169 2313 50-51 1.3925504614233901 2313 52-53 1.2444025627515174 2313 54-55 1.270714760555414 2313 56-57 1.201618251739255 2313 58-59 1.150669416403396 2313 60-61 1.1709464600406747 2313 62-63 1.1867633107564117 2313 64-65 1.1461484713190586 2313 66-67 1.136420887758824 2313 68-69 1.1861039363070844 2313 70-71 1.2196217246346421 2313 72-73 1.213263711696861 2313 74-75 1.136843403227573 2313 76-77 1.1244066255008605 2313 78-79 1.199327657818607 2313 80-81 1.3023267473197855 2313 82-83 1.251503494538607 2313 84-85 1.31450148242822 2313 86-87 1.4387928723726908 2313 88-89 1.4578801120546707 2313 90-91 1.5308027760026732 2313 92-93 1.5015124787880225 2313 94-95 1.5476969086803578 2313 96-97 1.6411015440420123 2313 98-99 1.610967843246197 2313 100 1.6744094407360777 2314 1 0.4379330512135162 2314 2 0.4675327609105544 2314 3 0.5598708520709756 2314 4 0.5567755930818592 2314 5 0.5469269744500949 2314 6 0.5188222130350795 2314 7 0.5796277724481911 2314 8 0.5908964889591957 2314 9 0.7310849968923279 2314 10-11 0.7100547372672423 2314 12-13 0.46350984134932816 2314 14-15 0.865454182780816 2314 16-17 0.9541622665423901 2314 18-19 0.9483957160339358 2314 20-21 0.9965233049774511 2314 22-23 0.8869374448414149 2314 24-25 0.8798604352685402 2314 26-27 0.9706147975616304 2314 28-29 1.0623829395058877 2314 30-31 1.0455634013790558 2314 32-33 1.049339462756656 2314 34-35 1.1117122375991286 2314 36-37 1.2563530069853286 2314 38-39 1.159392033161616 2314 40-41 1.2581204561518575 2314 42-43 1.2438326833482378 2314 44-45 1.3623211855089465 2314 46-47 1.3181217585511789 2314 48-49 1.373725238225063 2314 50-51 1.3264789664064054 2314 52-53 1.0861472084675015 2314 54-55 1.0769880645827214 2314 56-57 1.1140569679074446 2314 58-59 1.1553611905419743 2314 60-61 1.0788604916222866 2314 62-63 1.0482340222383968 2314 64-65 1.0502598712618152 2314 66-67 1.1568741090428531 2314 68-69 1.0904488377167922 2314 70-71 1.1350119244654238 2314 72-73 1.0445876920973447 2314 74-75 1.1279532342477196 2314 76-77 1.1816190379662714 2314 78-79 1.17749281750001 2314 80-81 1.2023154667143778 2314 82-83 1.1855650196042404 2314 84-85 1.1821314672665224 2314 86-87 1.3275468702365174 2314 88-89 1.522616656930051 2314 90-91 1.5983034617096195 2314 92-93 1.727796831057539 2314 94-95 1.6783336199623307 2314 96-97 1.71362881100017 2314 98-99 1.8010689275188376 2314 100 1.8005194656538883 2315 1 0.3946643571841868 2315 2 0.43457670487988054 2315 3 0.49288978011890094 2315 4 0.3482488638866883 2315 5 0.35913445120304743 2315 6 -0.3240535647857854 2315 7 0.21065694352742526 2315 8 0.3680899923950136 2315 9 0.584059394737956 2315 10-11 0.6386820029311195 2315 12-13 0.6233808158565566 2315 14-15 0.8135068000149701 2315 16-17 0.6979410555758108 2315 18-19 0.7764184882695417 2315 20-21 0.7941211735663458 2315 22-23 0.664674694048287 2315 24-25 0.754405223331986 2315 26-27 0.7762853472872493 2315 28-29 0.8784734752107255 2315 30-31 0.8954097920687545 2315 32-33 0.9697777724711685 2315 34-35 0.9734336878952021 2315 36-37 1.0075440045802821 2315 38-39 1.0286357519487765 2315 40-41 1.1192685552338162 2315 42-43 1.0427565819918598 2315 44-45 1.048389361194424 2315 46-47 1.0823183594847237 2315 48-49 1.1374222755563963 2315 50-51 1.1242446368386183 2315 52-53 0.9811482277083243 2315 54-55 0.8117221647648307 2315 56-57 0.8003454894477784 2315 58-59 0.6992882101868716 2315 60-61 0.6504309722384036 2315 62-63 0.8470953200596654 2315 64-65 0.961306475653096 2315 66-67 1.0089057656233322 2315 68-69 1.0206188633582656 2315 70-71 1.0133399943577714 2315 72-73 1.1008483167224696 2315 74-75 1.0099788205823828 2315 76-77 1.0296500048077277 2315 78-79 1.0668575027715548 2315 80-81 1.0207313856741074 2315 82-83 1.2031625175601128 2315 84-85 1.2307095924148328 2315 86-87 1.301367296726653 2315 88-89 1.4042061844678564 2315 90-91 1.46289234606353 2315 92-93 1.6830417629898804 2315 94-95 1.6755134190159495 2315 96-97 1.7955606316987591 2315 98-99 1.8407563799184814 2315 100 1.8431454277742887 2316 1 0.1143836262832707 2316 2 0.0713920878491976 2316 3 0.1972374260074936 2316 4 0.15549585158350965 2316 5 0.14079244074346065 2316 6 0.2330737890904686 2316 7 0.1895362832663139 2316 8 0.27533983131556994 2316 9 0.39529941839429483 2316 10-11 0.3354176897879739 2316 12-13 0.42220486243085276 2316 14-15 0.6296127162160374 2316 16-17 0.6133071820972447 2316 18-19 0.5110086527822091 2316 20-21 0.4831398911153073 2316 22-23 0.4350116352084328 2316 24-25 0.5226010277415654 2316 26-27 0.5516793893466243 2316 28-29 0.7347590126281887 2316 30-31 0.6653242671143715 2316 32-33 0.7133645031698563 2316 34-35 0.6900913452827595 2316 36-37 0.727272091897099 2316 38-39 0.8214006541488601 2316 40-41 0.7879167489100922 2316 42-43 0.8967410054920606 2316 44-45 0.7540714238813706 2316 46-47 0.6540117923816169 2316 48-49 0.7640473995993275 2316 50-51 0.5945010823678984 2316 52-53 0.40033668419101076 2316 54-55 0.34174922493564 2316 56-57 0.432387718128858 2316 58-59 0.45507274056387104 2316 60-61 0.4909045634399476 2316 62-63 0.5628677753571836 2316 64-65 0.6189806178168737 2316 66-67 0.5677060823737108 2316 68-69 0.560236323194232 2316 70-71 0.5171749637644396 2316 72-73 0.6845833933586576 2316 74-75 0.6482898467642642 2316 76-77 0.74629347956175 2316 78-79 0.9156507149778434 2316 80-81 1.04141664643468 2316 82-83 0.9485028243400251 2316 84-85 1.0817899563603106 2316 86-87 1.289453223203882 2316 88-89 1.3966741037917494 2316 90-91 1.4989263026684938 2316 92-93 1.5141620176129784 2316 94-95 1.586147102021009 2316 96-97 1.6546276554444574 2316 98-99 1.710015720174706 2316 100 1.6162119747483494 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 14.0 1 137.0 2 66.0 3 52.0 4 45.0 5 53.0 6 61.0 7 390.0 8 1227.0 9 2066.0 10 2610.0 11 3136.0 12 3779.0 13 4558.0 14 5440.0 15 7031.0 16 9817.0 17 13741.0 18 17471.0 19 21895.0 20 26375.0 21 31397.0 22 34057.0 23 34865.0 24 34578.0 25 35688.0 26 39466.0 27 45348.0 28 52388.0 29 62330.0 30 74270.0 31 90720.0 32 112903.0 33 144617.0 34 196646.0 35 268211.0 36 378342.0 37 543580.0 38 505875.0 39 130351.0 40 122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.926320159166046 18.667325761930968 12.109748920208052 23.296605158694934 2 16.573000935406466 21.129815449967353 39.39647520285903 22.900708411767155 3 19.205981458704564 26.89383898850794 30.348168868204166 23.552010684583333 4 12.999108380642763 17.042092814951676 37.9198183099844 32.03898049442116 5 12.767846617117243 37.50091141955896 34.560484527673424 15.170757435650373 6 29.13323917971844 36.776381529350985 19.281735607379428 14.808643683551153 7 26.10189213249699 33.59479271612832 22.895123590511936 17.408191560862754 8 25.767982643582034 34.49093906918507 21.288978497372067 18.45209978986084 9 27.335874075164522 15.834723653434091 20.942821041267973 35.886581230133416 10-11 24.591014188034478 25.815168775760217 29.111676035051786 20.48214100115351 12-13 25.14205702317996 24.408557191348212 28.495079843288313 21.954305942183517 14-15 22.908722706620186 25.6611430308019 26.819280416906295 24.610853845671627 16-17 21.751162011862178 28.144211778982843 27.587216046819123 22.51741016233586 18-19 21.247076459215222 27.455765741978894 29.94227018371675 21.354887615089133 20-21 22.199794625371876 26.944687856458533 29.696837339751237 21.158680178418358 22-23 22.194378535731758 26.650678219960504 29.51224448354296 21.642698760764777 24-25 21.75546855928694 26.463300727619437 29.857557758721576 21.923672954372044 26-27 21.590012256415452 26.7372297775533 29.64771535816585 22.0250426078654 28-29 21.353195693649496 27.5897894521668 29.53556827473426 21.521446579449442 30-31 22.431498536324447 26.83576086854686 29.611148633973794 21.121591961154895 32-33 22.21053768481936 26.937988325835395 29.238068597642 21.613405391703246 34-35 22.136547591384822 27.05243192007103 29.533857852420063 21.277162636124082 36-37 21.912746555749234 26.82050577914547 29.540744985751456 21.726002679353847 38-39 21.797538699613927 26.900032690822666 29.56085101076651 21.741577598796898 40-41 21.764578516899135 26.32886736206735 29.987531713722422 21.919022407311097 42-43 21.379491636940774 26.67590841301032 29.456814285874916 22.487785664173995 44-45 20.85120832653943 27.02341174732712 29.926278271794626 22.199101654338822 46-47 20.861676574980613 26.505709598112244 29.68580384622397 22.946809980683174 48-49 21.03746584279319 26.656268859088804 29.623835400684186 22.682429897433817 50-51 20.973549880670454 26.90740303164682 29.46145908629462 22.657588001388103 52-53 20.7401077864921 27.16187227488111 29.174051501019765 22.923968437607023 54-55 20.456400314732985 26.79735332594322 29.644109098802506 23.102137260521285 56-57 20.98290176690318 26.620535906732513 29.082723047267756 23.31383927909655 58-59 20.671222179302127 26.715162775544588 29.274805849999325 23.338809195153956 60-61 21.108919769822286 26.61011200371784 29.007663107426435 23.273305119033445 62-63 21.161394669859863 26.355946539483888 28.99866558250569 23.48399320815056 64-65 20.83257807240147 26.117320529559436 28.977300604299604 24.07280079373949 66-67 20.905836261390803 26.08953722608238 28.634510962156508 24.37011555037031 68-69 20.487799891471298 26.242274570804756 28.82452510173501 24.445400435988937 70-71 20.5286352919448 25.8841114654641 29.030965922934303 24.5562873196568 72-73 20.55040203482047 26.064832981457375 28.768334196907524 24.61643078681463 74-75 20.68535161101172 25.965453715972536 28.630376839127784 24.718817833887957 76-77 20.56802477099993 25.592492385118387 28.876792825308883 24.9626900185728 78-79 20.475909001865276 25.636995460313944 29.070326806292535 24.816768731528246 80-81 20.627932247900144 25.667757414761777 28.917873295035353 24.78643704230273 82-83 20.923576747167512 25.302906646647493 29.129103039960153 24.644413566224838 84-85 21.04292512873553 25.42885014658246 28.743925187160702 24.784299537521314 86-87 20.850806438562834 25.4196057737005 29.026575926607595 24.703011861129077 88-89 21.07130780807765 24.782097286468797 29.148379282825093 24.99821562262846 90-91 21.714673717236423 24.470845279662058 29.03300704403977 24.781473959061746 92-93 21.853266752136083 24.473393811519156 28.833594590828515 24.839744845516247 94-95 21.623205915241314 24.14581020775711 28.977513933266145 25.25346994373543 96-97 21.684081845006645 23.879796343178814 28.97852513197881 25.45759667983573 98-99 21.75943948387431 23.897168746857822 28.79699979539103 25.546391973876837 100 22.05584612501491 23.767006831694975 28.823312946147162 25.35383409714295 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3023.0 1 2066.0 2 2628.5 3 3894.0 4 2553.5 5 1528.0 6 2052.5 7 3258.5 8 4761.5 9 5522.0 10 4523.0 11 3379.5 12 3343.5 13 3640.5 14 3988.5 15 4339.0 16 4674.0 17 5018.5 18 5497.0 19 5931.5 20 6329.0 21 6952.5 22 7801.5 23 9006.0 24 10421.0 25 12097.0 26 14282.5 27 17017.0 28 20142.0 29 23868.5 30 28844.0 31 33733.5 32 39425.5 33 45604.5 34 51601.5 35 57366.0 36 63427.5 37 72058.0 38 77372.0 39 79014.0 40 80796.0 41 83216.5 42 86446.0 43 91040.5 44 97562.5 45 105327.0 46 113164.0 47 120297.5 48 126841.5 49 131443.5 50 133677.0 51 132604.5 52 126543.5 53 118689.5 54 110282.0 55 98149.5 56 85532.0 57 72941.0 58 60425.5 59 49514.0 60 38983.5 61 30044.5 62 23341.5 63 18021.0 64 13294.0 65 9394.5 66 6754.5 67 4998.0 68 3797.5 69 2934.0 70 2261.5 71 1755.5 72 1338.5 73 983.5 74 744.0 75 557.5 76 413.5 77 288.5 78 200.0 79 150.0 80 111.0 81 84.5 82 69.5 83 47.5 84 29.5 85 27.5 86 25.5 87 19.5 88 19.0 89 14.5 90 9.5 91 9.5 92 8.0 93 5.0 94 4.0 95 2.5 96 1.5 97 2.0 98 2.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21970775122133668 2 0.14892438578909828 3 0.022072964773864518 4 0.020744499301363413 5 0.025070527891302912 6 0.05129920516888884 7 0.05702182566581668 8 0.050141055782605824 9 0.038900194092211855 10-11 0.04097805034407256 12-13 0.039411142350866124 14-15 0.01955228669783678 16-17 0.01727005114251437 18-19 0.016793166101103714 20-21 0.020233551042709144 22-23 0.023231114160147535 24-25 0.020386835520305423 26-27 0.017729904575303213 28-29 0.01764474653219417 30-31 0.01602674371312231 32-33 0.02350361989809648 34-35 0.024253010677456076 36-37 0.023231114160147535 38-39 0.02191968029626824 40-41 0.022873450379089545 42-43 0.02885154500534452 44-45 0.03752063379384532 46-47 0.041880725601028436 48-49 0.03832111939907035 50-51 0.02651821462415668 52-53 0.041318682516508734 54-55 0.03975177452330231 56-57 0.04201697847000291 58-59 0.045116731239172154 60-61 0.023214082551525725 62-63 0.02484911697921939 64-65 0.025547412932713565 66-67 0.03045251621579457 68-69 0.03442088102467608 70-71 0.037639855054197986 72-73 0.04116539803891246 74-75 0.023810188853289043 76-77 0.017423335620110652 78-79 0.016793166101103714 80-81 0.01963744474094583 82-83 0.018104599964983013 84-85 0.01156446225420834 86-87 0.012722611640491354 88-89 0.017729904575303213 90-91 0.018445232137419193 92-93 0.01890508557020804 94-95 0.022345470511813462 96-97 0.023248145768769345 98-99 0.0290048294829408 100 0.0295668725674605 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2935718.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.50306681736588 #Duplication Level Percentage of deduplicated Percentage of total 1 81.18006805154785 54.799035579219435 2 12.116492583960909 16.35800816974463 3 3.116435626639199 6.311068870111361 4 1.3032671871375794 3.5189812805691396 5 0.6396238506961516 2.1588285765761586 6 0.3958563971549856 1.603291249634083 7 0.27276592493213064 1.2888775519335955 8 0.18290054507059791 0.9877078171866569 9 0.13436479465826087 0.8163032140546409 >10 0.6199888605290302 7.145669823097977 >50 0.025136351720564543 1.1573749962818771 >100 0.011899171785636986 1.434432855739572 >500 6.5490227298718E-4 0.31807592605810925 >1k 3.274511364667964E-4 0.6018907203643931 >5k 1.0915037882226545E-4 0.467719707782246 >10k+ 1.0915037882226545E-4 1.0327336616461644 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15282 0.5205540859169716 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12751 0.4343400830733742 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7328 0.24961525596123332 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5368 0.18285135016374188 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4736 0.16132339686577524 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4036 0.1374791447952426 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.406321724361808E-5 2 0.0 0.0 0.0 0.0 3.406321724361808E-5 3 0.0 0.0 0.0 0.0 3.406321724361808E-5 4 0.0 0.0 0.0 0.0 3.406321724361808E-5 5 0.0 0.0 0.0 0.0 3.406321724361808E-5 6 3.406321724361808E-5 0.0 0.0 0.0 3.406321724361808E-5 7 3.406321724361808E-5 0.0 0.0 0.0 3.406321724361808E-5 8 3.406321724361808E-5 0.0 0.0 0.0 3.406321724361808E-5 9 3.406321724361808E-5 0.0 0.0 0.0 3.406321724361808E-5 10-11 3.406321724361808E-5 0.0 0.0 6.812643448723617E-5 3.406321724361808E-5 12-13 3.406321724361808E-5 0.0 0.0 2.2141091208351755E-4 3.406321724361808E-5 14-15 3.406321724361808E-5 0.0 0.0 2.384425207053266E-4 5.109482586542712E-5 16-17 3.406321724361808E-5 0.0 0.0 2.8953734657075373E-4 6.812643448723617E-5 18-19 3.406321724361808E-5 0.0 0.0 4.7688504141065314E-4 6.812643448723617E-5 20-21 3.406321724361808E-5 0.0 0.0 0.0014306551242319596 6.812643448723617E-5 22-23 3.406321724361808E-5 0.0 0.0 0.004598534327888442 6.812643448723617E-5 24-25 3.406321724361808E-5 0.0 0.0 0.011956189252509948 6.812643448723617E-5 26-27 3.406321724361808E-5 0.0 0.0 0.027063226100054568 6.812643448723617E-5 28-29 3.406321724361808E-5 0.0 0.0 0.07536486815150502 6.812643448723617E-5 30-31 3.406321724361808E-5 0.0 0.0 0.20746202462225596 6.812643448723617E-5 32-33 3.406321724361808E-5 0.0 0.0 0.4652013578960922 1.0218965173085425E-4 34-35 3.406321724361808E-5 0.0 0.0 0.8478845720195196 1.1922126035266329E-4 36-37 3.406321724361808E-5 0.0 0.0 1.4115115961410463 1.5328447759628138E-4 38-39 3.406321724361808E-5 0.0 0.0 2.1804887254157244 1.7031608621809042E-4 40-41 3.406321724361808E-5 0.0 0.0 2.9921300342880346 1.7031608621809042E-4 42-43 3.406321724361808E-5 0.0 0.0 3.7472264025359383 1.7031608621809042E-4 44-45 3.406321724361808E-5 0.0 0.0 4.624575657471188 1.7031608621809042E-4 46-47 3.406321724361808E-5 0.0 0.0 5.592958860490006 1.7031608621809042E-4 48-49 3.406321724361808E-5 0.0 0.0 6.575597519925279 1.7031608621809042E-4 50-51 3.406321724361808E-5 0.0 0.0 7.541494108085313 1.7031608621809042E-4 52-53 3.406321724361808E-5 0.0 0.0 8.458697327195596 1.8734769483989947E-4 54-55 3.406321724361808E-5 0.0 0.0 9.451316509283249 2.043793034617085E-4 56-57 3.406321724361808E-5 0.0 0.0 10.520697151429395 2.043793034617085E-4 58-59 3.406321724361808E-5 0.0 0.0 11.66002661018531 2.043793034617085E-4 60-61 3.406321724361808E-5 0.0 0.0 12.77104953541178 2.043793034617085E-4 62-63 3.406321724361808E-5 0.0 0.0 13.868293889263207 2.5547412932713564E-4 64-65 3.406321724361808E-5 0.0 0.0 14.93867939631804 2.7250573794894466E-4 66-67 3.406321724361808E-5 0.0 0.0 16.080291090629277 2.7250573794894466E-4 68-69 3.406321724361808E-5 0.0 0.0 17.288666690738005 2.7250573794894466E-4 70-71 3.406321724361808E-5 0.0 0.0 18.519541045836146 3.0656895519256275E-4 72-73 3.406321724361808E-5 0.0 0.0 19.65544715125908 3.576637810579899E-4 74-75 3.406321724361808E-5 0.0 0.0 20.743187867499536 3.9172699830160795E-4 76-77 3.406321724361808E-5 0.0 0.0 21.89057327713357 4.08758606923417E-4 78-79 3.406321724361808E-5 0.0 0.0 23.09108027405902 4.08758606923417E-4 80-81 3.406321724361808E-5 0.0 0.0 24.249587324123095 4.08758606923417E-4 82-83 3.406321724361808E-5 0.0 0.0 25.39758587166751 4.428218241670351E-4 84-85 3.406321724361808E-5 0.0 0.0 26.490129501539315 4.428218241670351E-4 86-87 3.406321724361808E-5 0.0 0.0 27.571772901893166 4.428218241670351E-4 88 3.406321724361808E-5 0.0 0.0 28.397005434445678 4.428218241670351E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5400 0.0 59.160496 1 TACCTGG 2555 0.0 48.074158 2 GTATCAA 10085 0.0 43.4046 1 ACCTGGG 3005 0.0 39.73199 3 ATCAACG 12300 0.0 35.426292 3 CAACGCA 12365 0.0 35.20205 5 TATCAAC 12680 0.0 34.33085 2 TCAACGC 12805 0.0 34.06587 4 AACGCAG 13165 0.0 33.634773 6 GTACATG 9520 0.0 30.191784 1 ACGCAGA 14735 0.0 29.5406 7 GTGGTAT 1940 0.0 29.388643 1 TACATGG 9730 0.0 29.116938 2 CCTGGGG 2900 0.0 29.0139 4 CGCAGAG 15095 0.0 28.742668 8 CATGGGG 7495 0.0 26.905233 4 ATGGGGG 5815 0.0 26.837337 5 ACATGGG 10125 0.0 26.741055 3 GCAGAGT 15870 0.0 26.331068 9 TGGGGGG 6355 0.0 26.184614 6 >>END_MODULE