FastQCFastQC Report
Mon 6 Jun 2016
ERR1211442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211442_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2935718
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143680.4894203053563047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123330.4201016582655419No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71640.24402888833328No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51630.17586839062880016No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42840.14592682267165988No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35300.12024315686997185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59650.056.5239451
TACCTGG26400.048.600222
ACCTGGG30950.040.848033
GTATCAA106100.040.1547161
ATCAACG128350.032.662433
TATCAAC131700.032.295522
CAACGCA130300.032.1675955
TCAACGC135350.031.0089824
AACGCAG138200.030.6327026
CCTGGGG29950.030.6007544
CTGGGGG20050.028.1232175
GTGGTAT22750.028.1113221
TGGGGGG56650.027.1216686
ACGCAGA154300.027.0710077
GTACATG102050.026.8645321
CGCAGAG156000.026.6555238
TACATGG102900.026.0795572
TAACGCA3250.024.5844154
GTACCTG53850.024.5382941
ACATGGG106000.024.2527483
Produced by FastQC (version 0.11.5)