##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211442_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2935718 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09727501074695 33.0 31.0 34.0 30.0 34.0 2 32.22415913245073 33.0 31.0 34.0 30.0 34.0 3 32.304134457056165 34.0 31.0 34.0 30.0 34.0 4 35.93943321531564 37.0 35.0 37.0 35.0 37.0 5 35.80774822377354 37.0 35.0 37.0 35.0 37.0 6 35.85158554057304 37.0 35.0 37.0 35.0 37.0 7 35.78503623304418 37.0 35.0 37.0 33.0 37.0 8 35.77497702435997 37.0 35.0 37.0 33.0 37.0 9 37.36109326577008 39.0 37.0 39.0 34.0 39.0 10-11 37.34684700642228 39.0 37.0 39.0 34.0 39.0 12-13 37.26463883792653 39.0 37.0 39.0 34.0 39.0 14-15 38.477781244656335 40.0 38.0 41.0 34.0 41.0 16-17 38.46034786038713 40.0 38.0 41.0 34.0 41.0 18-19 38.476276161402424 40.0 38.0 41.0 34.0 41.0 20-21 38.43871720648917 40.0 38.0 41.0 34.0 41.0 22-23 38.33216593010636 40.0 38.0 41.0 34.0 41.0 24-25 38.214171797154904 40.0 37.5 41.0 33.5 41.0 26-27 38.08367118367636 40.0 37.0 41.0 33.0 41.0 28-29 37.99333876755192 40.0 37.0 41.0 33.0 41.0 30-31 38.010175023622836 40.0 37.0 41.0 33.0 41.0 32-33 37.814693713769515 40.0 37.0 41.0 33.0 41.0 34-35 37.68362577740777 40.0 36.5 41.0 33.0 41.0 36-37 37.52555780221397 40.0 36.0 41.0 32.5 41.0 38-39 37.39810703889134 40.0 36.0 41.0 32.0 41.0 40-41 37.31808726178741 40.0 36.0 41.0 32.0 41.0 42-43 37.16265101075785 40.0 36.0 41.0 31.0 41.0 44-45 36.99299047115561 39.5 36.0 41.0 31.0 41.0 46-47 36.74364703966798 39.0 35.0 41.0 30.5 41.0 48-49 36.504221624829086 39.0 35.0 41.0 30.0 41.0 50-51 36.311511187382436 39.0 35.0 41.0 29.5 41.0 52-53 36.103849381991054 39.0 35.0 40.5 29.0 41.0 54-55 35.90131664553612 38.5 35.0 40.0 28.0 41.0 56-57 35.667233535373626 38.0 35.0 40.0 28.0 41.0 58-59 35.402946911113396 38.0 34.0 40.0 27.0 41.0 60-61 35.13948734176784 37.5 34.0 40.0 26.5 41.0 62-63 34.83401266742923 37.0 34.0 40.0 26.0 41.0 64-65 34.48479503140288 36.5 33.5 39.5 26.0 41.0 66-67 34.125162907336474 36.0 33.0 39.0 26.0 41.0 68-69 33.74672209660464 35.5 33.0 39.0 25.0 40.0 70-71 33.360880200346216 35.0 32.5 38.0 24.5 40.0 72-73 32.99506730551095 35.0 32.0 37.5 24.0 40.0 74-75 32.61573352753909 35.0 32.0 37.0 23.5 39.0 76-77 31.58043092013606 34.0 30.5 36.0 22.0 38.0 78-79 32.02142729649101 35.0 31.0 36.0 23.0 38.0 80-81 31.90434162954344 35.0 31.0 35.5 23.0 37.0 82-83 31.628066455974313 34.5 31.0 35.0 22.5 37.0 84-85 31.35128970153128 34.0 31.0 35.0 20.5 36.5 86-87 31.10146257235879 34.0 31.0 35.0 20.0 36.0 88-89 30.871753519922553 34.0 30.5 35.0 20.0 36.0 90-91 30.653726107207845 34.0 30.0 35.0 19.0 35.0 92-93 30.474508110111394 34.0 30.0 35.0 19.0 35.0 94-95 30.306314502959754 34.0 30.0 35.0 18.5 35.0 96-97 30.134232920191927 34.0 29.0 35.0 18.0 35.0 98-99 29.93354044904858 34.0 29.0 35.0 18.0 35.0 100 28.174078368562647 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.28246087133867803 1101 2 -0.24695615520025527 1101 3 -0.2878245917467446 1101 4 -0.15689218899440505 1101 5 -0.21797750617670175 1101 6 -0.27076565138150244 1101 7 -0.27195119682186686 1101 8 -0.33545694810149485 1101 9 -0.44981578966523017 1101 10-11 -0.4082061277156086 1101 12-13 -0.41456845259379804 1101 14-15 -0.7002307219098967 1101 16-17 -0.7372313617340964 1101 18-19 -0.7524434065501353 1101 20-21 -0.7077454655789737 1101 22-23 -0.7687597171020926 1101 24-25 -0.7308933141499523 1101 26-27 -0.7038567438842591 1101 28-29 -0.8144049771356237 1101 30-31 -0.6916558720727224 1101 32-33 -0.5499017258711447 1101 34-35 -0.4959562499479375 1101 36-37 -0.5168472393317103 1101 38-39 -0.5782755664603059 1101 40-41 -0.6827493218171341 1101 42-43 -0.5251987435825995 1101 44-45 -0.4759837601689654 1101 46-47 -0.5523236701990584 1101 48-49 -0.4384291527129207 1101 50-51 -0.5077955466718009 1101 52-53 -0.45751712735972916 1101 54-55 -0.4808248506990296 1101 56-57 -0.5218012008461415 1101 58-59 -0.44992993826632954 1101 60-61 -0.39891316011485145 1101 62-63 -0.483059514722882 1101 64-65 -0.39674800024312873 1101 66-67 -0.4419023857611961 1101 68-69 -0.5320896574308662 1101 70-71 -0.45646372206304164 1101 72-73 -0.5552944341385952 1101 74-75 -0.6680151030321113 1101 76-77 -0.46450228302911967 1101 78-79 -0.3950327167540202 1101 80-81 -0.31487552149530984 1101 82-83 -0.46689421524272845 1101 84-85 -0.6123245360760663 1101 86-87 -0.6071039764096504 1101 88-89 -0.5707255909651643 1101 90-91 -0.6062966984636837 1101 92-93 -0.8153861918600604 1101 94-95 -0.9925065835795799 1101 96-97 -0.8657241734720138 1101 98-99 -0.9268781659652134 1101 100 -0.719590164269114 1102 1 -0.2431800127751238 1102 2 -0.20030752215773973 1102 3 -0.2115064431647653 1102 4 -0.09309750775290127 1102 5 -0.15371002122753197 1102 6 -0.24966157975524084 1102 7 -0.2355986225322937 1102 8 -0.2010920652553594 1102 9 -0.41804781200395524 1102 10-11 -0.46232640877891384 1102 12-13 -0.44908628860554956 1102 14-15 -0.4593703857594633 1102 16-17 -0.6199391408877801 1102 18-19 -0.5862334946985399 1102 20-21 -0.6946249127951418 1102 22-23 -0.7018268387354283 1102 24-25 -0.732261579425149 1102 26-27 -0.7204243655599925 1102 28-29 -0.744055517219735 1102 30-31 -0.612488323133455 1102 32-33 -0.4907886010034872 1102 34-35 -0.5818166035911148 1102 36-37 -0.6214079958576306 1102 38-39 -0.6097948140526839 1102 40-41 -0.4847155388426998 1102 42-43 -0.5750347200130435 1102 44-45 -0.5844063300653559 1102 46-47 -0.6142896939715214 1102 48-49 -0.6478780298540983 1102 50-51 -0.8851952795493716 1102 52-53 -0.764603135485352 1102 54-55 -0.8417562540114218 1102 56-57 -0.6401312408245445 1102 58-59 -0.7430377496732916 1102 60-61 -0.9389702797340291 1102 62-63 -0.7300937043618205 1102 64-65 -0.5997077381252822 1102 66-67 -0.867673619737225 1102 68-69 -0.7873304399659915 1102 70-71 -1.0250544954602887 1102 72-73 -1.0406133625634801 1102 74-75 -0.8072502139219111 1102 76-77 -0.8373155867058806 1102 78-79 -0.6943166307157433 1102 80-81 -0.6098094914088001 1102 82-83 -0.5956781651062073 1102 84-85 -0.6413972160684231 1102 86-87 -0.8275941862242071 1102 88-89 -0.8235834461456228 1102 90-91 -0.7680043961511949 1102 92-93 -0.8265500569953836 1102 94-95 -0.9151520319442668 1102 96-97 -1.0939902383440803 1102 98-99 -0.8997039477947943 1102 100 -1.1308945710562313 1103 1 -0.2886200047382701 1103 2 -0.2464314450538403 1103 3 -0.35141539319605997 1103 4 -0.21768687059771707 1103 5 -0.14871376604877895 1103 6 -0.3018364285610957 1103 7 -0.2816960996554485 1103 8 -0.33366256278350903 1103 9 -0.43201982904967196 1103 10-11 -0.45043570732890714 1103 12-13 -0.5492542275800218 1103 14-15 -0.7074847938323714 1103 16-17 -0.6346289361293032 1103 18-19 -0.6290344812755606 1103 20-21 -0.6961353157139527 1103 22-23 -0.7532051541663023 1103 24-25 -0.7676419486447301 1103 26-27 -0.875661551709797 1103 28-29 -0.835534522852015 1103 30-31 -0.560023069802078 1103 32-33 -0.6050480619787564 1103 34-35 -0.6246426221733543 1103 36-37 -0.6488987926454755 1103 38-39 -0.7187805887217209 1103 40-41 -0.6560862265808325 1103 42-43 -0.7959719382470567 1103 44-45 -0.7028872183372883 1103 46-47 -0.71198711540886 1103 48-49 -0.8677853598738992 1103 50-51 -0.8624838493370177 1103 52-53 -0.8200989247028971 1103 54-55 -0.7883495976935322 1103 56-57 -0.7148553523671666 1103 58-59 -0.8896806417564136 1103 60-61 -0.672477722722185 1103 62-63 -0.5928038006427272 1103 64-65 -0.6743517638837986 1103 66-67 -0.6043984654411076 1103 68-69 -0.7917403141855672 1103 70-71 -0.7976178542930015 1103 72-73 -0.9542437966916353 1103 74-75 -1.030668095555619 1103 76-77 -0.9622613021829594 1103 78-79 -0.7165731603683554 1103 80-81 -0.6445295479328976 1103 82-83 -0.6728392795743225 1103 84-85 -0.6107103062436856 1103 86-87 -0.7705372647749726 1103 88-89 -0.7915906307469314 1103 90-91 -1.0150193919024026 1103 92-93 -0.8748544255543216 1103 94-95 -0.7843600542279106 1103 96-97 -0.9457423040666519 1103 98-99 -1.1068296532025208 1103 100 -0.8335381337642289 1104 1 -0.33394664212517 1104 2 -0.29463505942492674 1104 3 -0.3355185203710427 1104 4 -0.19908101944452739 1104 5 -0.17561455084249644 1104 6 -0.28848653536024216 1104 7 -0.22843452574804246 1104 8 -0.2626501308829674 1104 9 -0.47452048904439437 1104 10-11 -0.4912308809675139 1104 12-13 -0.5013266110009482 1104 14-15 -0.5927252119090269 1104 16-17 -0.6644216977872119 1104 18-19 -0.603438686041919 1104 20-21 -0.582016228666653 1104 22-23 -0.7210604113242525 1104 24-25 -0.6915370574838633 1104 26-27 -0.7489245656056909 1104 28-29 -0.6568514523165803 1104 30-31 -0.6501423488478437 1104 32-33 -0.6208114358717651 1104 34-35 -0.7186933697673652 1104 36-37 -0.6914984518482044 1104 38-39 -0.7398719199910673 1104 40-41 -0.803142050134241 1104 42-43 -0.6427121643252534 1104 44-45 -0.7914570097789593 1104 46-47 -0.8911306822603251 1104 48-49 -0.7983104156734484 1104 50-51 -0.5760251410066886 1104 52-53 -0.7183292388603846 1104 54-55 -0.7103442217365412 1104 56-57 -0.6199121119130808 1104 58-59 -0.5944800033615181 1104 60-61 -0.6188651516227495 1104 62-63 -0.660372260095059 1104 64-65 -0.5106445735413274 1104 66-67 -0.570198739038922 1104 68-69 -0.5175110080200156 1104 70-71 -0.756011187146342 1104 72-73 -0.7831881651366857 1104 74-75 -0.7129706371352889 1104 76-77 -0.6381678949302199 1104 78-79 -0.6064395414373074 1104 80-81 -0.6596449270098681 1104 82-83 -0.6324491026957411 1104 84-85 -0.6667423579872711 1104 86-87 -0.5708978618901988 1104 88-89 -0.6969938875208754 1104 90-91 -0.6380079079942753 1104 92-93 -0.8162723942105714 1104 94-95 -0.9611696397512262 1104 96-97 -1.0287996396079642 1104 98-99 -0.9750467074660882 1104 100 -0.9641960885005929 1105 1 -0.39582956062114505 1105 2 -0.3844795610078968 1105 3 -0.3619394039192265 1105 4 -0.12465255046549828 1105 5 -0.20675256953496302 1105 6 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1105 68-69 -0.7787922957358191 1105 70-71 -0.7819915793032024 1105 72-73 -0.7488515550838031 1105 74-75 -0.7939470062734877 1105 76-77 -0.7454373147409612 1105 78-79 -0.722141048028643 1105 80-81 -0.43109585201263556 1105 82-83 -0.682566659382406 1105 84-85 -0.6600696130641452 1105 86-87 -0.7496273168003178 1105 88-89 -0.740289075057678 1105 90-91 -0.6880385907996605 1105 92-93 -0.6497912091108979 1105 94-95 -0.7515681809696737 1105 96-97 -0.7304965955238423 1105 98-99 -0.8067941551814215 1105 100 -0.6988355910455901 1106 1 -0.37652940790145095 1106 2 -0.3265378916604007 1106 3 -0.32938144367680877 1106 4 -0.21140126103890822 1106 5 -0.25149979005757217 1106 6 -0.305476664418741 1106 7 -0.3404608398092108 1106 8 -0.41179401365300095 1106 9 -0.4278306880187017 1106 10-11 -0.487209468963151 1106 12-13 -0.5968870493458809 1106 14-15 -0.5918510482659372 1106 16-17 -0.672209884458276 1106 18-19 -0.7027535848610285 1106 20-21 -0.6283302667885167 1106 22-23 -0.6481962540067698 1106 24-25 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2311 22-23 0.7065350330418809 2311 24-25 0.7495797464347262 2311 26-27 0.6949374627488112 2311 28-29 0.603811706470367 2311 30-31 0.7329863705604467 2311 32-33 0.9170338501267494 2311 34-35 0.8730830017853464 2311 36-37 0.9097738422962109 2311 38-39 0.9956438007822968 2311 40-41 0.9741545032512562 2311 42-43 0.9247366004073996 2311 44-45 0.9261700858036974 2311 46-47 0.960586156454525 2311 48-49 0.9484108171849286 2311 50-51 0.8805060730260195 2311 52-53 0.9580225736680532 2311 54-55 1.0261097713577385 2311 56-57 0.917840941682087 2311 58-59 0.9995267863709074 2311 60-61 0.9177728601722919 2311 62-63 0.9855385097945799 2311 64-65 1.0246726300881974 2311 66-67 1.0136968364823602 2311 68-69 1.0587571596444363 2311 70-71 1.0229555868831497 2311 72-73 0.9820715577423016 2311 74-75 0.8247931230024719 2311 76-77 0.7155031108602401 2311 78-79 0.7928760205223462 2311 80-81 0.8560462918407872 2311 82-83 0.8530197769538042 2311 84-85 0.8454547360786329 2311 86-87 1.017736473991885 2311 88-89 0.9673480839104975 2311 90-91 0.9931092081984758 2311 92-93 1.0106553410880075 2311 94-95 1.0456769711819405 2311 96-97 1.0850827311774474 2311 98-99 1.1595050898947505 2311 100 1.2167165996445881 2312 1 0.225844004653446 2312 2 0.16444374012845486 2312 3 0.33119081271321704 2312 4 0.28131335107335786 2312 5 0.3637058556017223 2312 6 0.4056723713740382 2312 7 0.46527365223668227 2312 8 0.31414952789995 2312 9 0.4901558945686162 2312 10-11 0.3324194070790014 2312 12-13 0.4514779758067533 2312 14-15 0.7037849500534108 2312 16-17 0.7952889574918416 2312 18-19 0.8548200613948751 2312 20-21 0.8890060258511383 2312 22-23 0.4666994967819278 2312 24-25 0.4962259033241523 2312 26-27 0.7223044157387761 2312 28-29 0.7998760705861798 2312 30-31 0.7718031712425741 2312 32-33 0.6712552775530085 2312 34-35 0.7717985351100154 2312 36-37 0.8251369522952459 2312 38-39 0.8452280351074322 2312 40-41 0.8565914843417985 2312 42-43 0.8179712852749645 2312 44-45 0.7301691334311045 2312 46-47 0.7826658462713993 2312 48-49 0.8578961770485662 2312 50-51 0.7966162451470282 2312 52-53 0.7870724902756265 2312 54-55 0.8610296611888089 2312 56-57 0.8246632476202151 2312 58-59 0.8707465092576001 2312 60-61 0.8908140554716866 2312 62-63 0.8599751223791401 2312 64-65 0.8853335399789088 2312 66-67 0.8921675526202861 2312 68-69 0.8691846425332344 2312 70-71 0.7370876715501922 2312 72-73 0.8130408162077742 2312 74-75 0.9241066011485444 2312 76-77 0.8430968705065993 2312 78-79 0.8058044386346559 2312 80-81 0.701798024098057 2312 82-83 0.7320818973308185 2312 84-85 0.5947231081356641 2312 86-87 0.8660182151587144 2312 88-89 0.4810535551076107 2312 90-91 0.7967832207898944 2312 92-93 0.8389700802902631 2312 94-95 0.9771093347808879 2312 96-97 1.076422601370819 2312 98-99 1.0403072045766812 2312 100 1.1959905311965962 2313 1 -0.43708384715828785 2313 2 -0.10466116136505832 2313 3 0.20256844326753054 2313 4 0.014418506214752824 2313 5 0.24725646016326408 2313 6 0.3733899354039991 2313 7 0.10336328718042864 2313 8 0.04404128539810159 2313 9 0.1625897199295352 2313 10-11 0.328325047235424 2313 12-13 0.5273027995317037 2313 14-15 0.4953995273795826 2313 16-17 0.681580595592969 2313 18-19 0.6099798760808639 2313 20-21 0.7996370819499532 2313 22-23 0.8174436905349225 2313 24-25 0.6845105182742444 2313 26-27 0.8566017237826316 2313 28-29 0.7177865218701314 2313 30-31 0.746261286062925 2313 32-33 0.8735782090679223 2313 34-35 0.8611053258396595 2313 36-37 0.7399676745922008 2313 38-39 0.5095489047105488 2313 40-41 0.6736097543845787 2313 42-43 0.6411726157619455 2313 44-45 0.9051388475280859 2313 46-47 0.9409326822674302 2313 48-49 0.9201865182726223 2313 50-51 0.9136979900996849 2313 52-53 0.8525075852920949 2313 54-55 0.9104898962977401 2313 56-57 1.0053305586867651 2313 58-59 0.9050067142183025 2313 60-61 0.9873345871818415 2313 62-63 0.9838922213775021 2313 64-65 0.985840882943414 2313 66-67 1.064804437899845 2313 68-69 0.9791079942979977 2313 70-71 0.8531041190102329 2313 72-73 1.00106183090314 2313 74-75 0.6809600897588624 2313 76-77 0.8443466213038562 2313 78-79 0.6505601743102751 2313 80-81 0.7928034059333164 2313 82-83 0.8309583489417527 2313 84-85 0.9189352144090925 2313 86-87 0.9509677932016167 2313 88-89 0.9928142332978425 2313 90-91 1.1079121237927119 2313 92-93 0.859225641543631 2313 94-95 0.8382218576693816 2313 96-97 1.0328848947724971 2313 98-99 1.1821373983356551 2313 100 1.308137747041343 2314 1 -0.4143820408955001 2314 2 -0.20388519387455517 2314 3 0.08190215434807158 2314 4 -0.25155634612078615 2314 5 0.10076119589800214 2314 6 0.16824612063766153 2314 7 0.07044699869271653 2314 8 0.13922525974771105 2314 9 0.148182965947683 2314 10-11 0.36585370601775224 2314 12-13 0.49976352112306444 2314 14-15 0.5291670030940949 2314 16-17 0.7326534777240639 2314 18-19 0.7783473864300632 2314 20-21 0.43668317103392695 2314 22-23 0.6144113978590227 2314 24-25 0.8048536967422137 2314 26-27 0.7119215222734994 2314 28-29 0.6356973129270997 2314 30-31 0.7020866079683898 2314 32-33 0.7790717383022709 2314 34-35 0.8074343719149226 2314 36-37 0.7979199990013726 2314 38-39 0.7548818191621152 2314 40-41 0.8243870563934692 2314 42-43 0.6489363334726832 2314 44-45 0.6720553595868708 2314 46-47 0.7194650386077086 2314 48-49 0.5159810347836853 2314 50-51 0.7127672106263319 2314 52-53 0.8230203810468808 2314 54-55 0.88858526851768 2314 56-57 0.7330799373899595 2314 58-59 0.7922534520666176 2314 60-61 0.8552351259975666 2314 62-63 0.6606625659925385 2314 64-65 0.7618675632629888 2314 66-67 0.8352141303341867 2314 68-69 0.8479149066008276 2314 70-71 0.9613666090425568 2314 72-73 1.037261623377681 2314 74-75 1.0332029991094274 2314 76-77 0.917116338852253 2314 78-79 0.8572272633127618 2314 80-81 0.8038147526366437 2314 82-83 0.7715366020383421 2314 84-85 0.6947663485346247 2314 86-87 0.8583035073157497 2314 88-89 0.8825739477674261 2314 90-91 1.0057632587377974 2314 92-93 1.1026408530749983 2314 94-95 1.0896686718521416 2314 96-97 1.2354156832891725 2314 98-99 1.2984238371639236 2314 100 1.285220690813059 2315 1 -0.11293467372337318 2315 2 0.0517831184931552 2315 3 0.029508655974559872 2315 4 -0.1333287042191884 2315 5 0.18491771874549556 2315 6 0.33764236395553127 2315 7 0.3635153474909387 2315 8 0.33197409575660686 2315 9 0.505531963498342 2315 10-11 0.5043532795637802 2315 12-13 0.5698162142918051 2315 14-15 0.5899703682611914 2315 16-17 0.6086733400384148 2315 18-19 0.7591742461151725 2315 20-21 0.72415891604021 2315 22-23 0.7537062504442602 2315 24-25 0.7177611501272025 2315 26-27 0.7867952697485663 2315 28-29 0.7954080564485935 2315 30-31 0.808201329738246 2315 32-33 0.8126860069506492 2315 34-35 0.7938586943005674 2315 36-37 0.7979688140830561 2315 38-39 0.7868328720989055 2315 40-41 0.7574218041437959 2315 42-43 0.7566304265314017 2315 44-45 0.616104462135155 2315 46-47 0.6349040963681531 2315 48-49 0.7346705667962041 2315 50-51 0.6155531054180798 2315 52-53 0.6063703476673155 2315 54-55 0.5042129423298789 2315 56-57 0.6910868763634213 2315 58-59 0.7245095314652445 2315 60-61 0.7647720787884111 2315 62-63 0.7596726594146617 2315 64-65 0.8893663417559665 2315 66-67 0.7264862197236326 2315 68-69 0.7968667609185474 2315 70-71 0.34981420356641024 2315 72-73 0.599981206496885 2315 74-75 0.7926457794718402 2315 76-77 0.8330313436591794 2315 78-79 0.7449993565441453 2315 80-81 0.7694070129150425 2315 82-83 0.7381808725263248 2315 84-85 0.6140424751121323 2315 86-87 0.667042740313299 2315 88-89 0.7525000607173915 2315 90-91 0.8539465467137823 2315 92-93 0.8718174963239882 2315 94-95 0.9059350322493707 2315 96-97 1.0643637951172913 2315 98-99 0.969263741323644 2315 100 0.7932159684058995 2316 1 0.5106098794398974 2316 2 0.550044900807805 2316 3 0.5316639753586472 2316 4 0.31853734822922064 2316 5 0.39417667100013887 2316 6 0.3837729594086241 2316 7 0.4688746781059123 2316 8 0.458018172736125 2316 9 0.4471159740707975 2316 10-11 0.4514756452682036 2316 12-13 0.6530321918441473 2316 14-15 0.8083658024232037 2316 16-17 0.8045493241515089 2316 18-19 0.837415426133802 2316 20-21 0.8619234331781271 2316 22-23 0.8854129459406224 2316 24-25 0.9079002957242039 2316 26-27 0.9729088989507346 2316 28-29 0.9452277385929904 2316 30-31 0.7764191502980964 2316 32-33 0.8187982810867069 2316 34-35 0.6591469706564581 2316 36-37 0.6026421314495423 2316 38-39 0.5194864825123986 2316 40-41 0.5952562497483243 2316 42-43 0.6885100619745259 2316 44-45 0.6711690144283651 2316 46-47 0.7817343292161283 2316 48-49 0.4599503835418801 2316 50-51 0.44439311590587494 2316 52-53 0.6086331023374427 2316 54-55 0.6796956956596389 2316 56-57 0.7972793080986591 2316 58-59 0.7125732654278494 2316 60-61 0.7377362315575695 2316 62-63 0.7592758396167056 2316 64-65 0.8478550961768363 2316 66-67 0.7925041007176716 2316 68-69 0.7441255885423814 2316 70-71 0.6794128747197803 2316 72-73 0.6995558597645655 2316 74-75 0.8352484264656539 2316 76-77 0.7254690752843658 2316 78-79 0.6909742516661375 2316 80-81 0.695411804734551 2316 82-83 0.5021749431567208 2316 84-85 0.6121126816465505 2316 86-87 0.6031356467038016 2316 88-89 0.4782460672405193 2316 90-91 0.6585981124765254 2316 92-93 0.6515689137055176 2316 94-95 0.5522426387264119 2316 96-97 0.7879723391472382 2316 98-99 0.8726379000656728 2316 100 0.6592225620812364 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 20.0 13 102.0 14 442.0 15 1443.0 16 3197.0 17 6526.0 18 10500.0 19 14173.0 20 18741.0 21 23541.0 22 27989.0 23 28192.0 24 28165.0 25 29102.0 26 33029.0 27 39210.0 28 47550.0 29 58094.0 30 70469.0 31 87060.0 32 108127.0 33 139759.0 34 195872.0 35 271639.0 36 402393.0 37 627465.0 38 575666.0 39 87239.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.07573085922776 19.402641732946275 12.826118517417868 23.695508890408092 2 16.26719596364501 21.434415703415656 39.1420088714243 23.156379461515037 3 18.726389932548017 26.76323134579002 30.95120171624114 23.55917700542082 4 12.841875959474 17.45873026796793 37.892076933717 31.807316838841075 5 12.589240882765123 37.702693219838984 34.55106530398959 15.157000593406309 6 29.214079826468346 37.153602628045334 18.973416383998735 14.65890116148758 7 25.972897904904908 33.783876767287275 22.721491262432238 17.521734065375576 8 25.833649554825993 34.898760298204245 20.96422461922277 18.303365527746987 9 27.20710489302772 15.839432742201346 20.7291903339173 36.22427203085364 10-11 24.590540028667817 26.013252053346125 28.988202456930146 20.408005461055915 12-13 25.04960901234507 24.46400891914774 28.48079329456129 22.0055887739459 14-15 22.8299385073666 25.826088511581922 26.637900274805055 24.70607270624643 16-17 21.71697155259084 28.30814157570786 27.496278164556475 22.47860870714482 18-19 21.200622811864083 27.51711846982578 29.902463383744625 21.379795334565515 20-21 22.151296914638415 26.98175147983415 29.635514997611317 21.231436607916123 22-23 22.063665515557012 26.76450871643666 29.40307958734456 21.768746180661765 24-25 21.665443512682508 26.56477558374591 29.785760988842075 21.984019914729505 26-27 21.600736719513495 26.825576171000133 29.53627682777325 22.03741028171312 28-29 21.36222899670725 27.688240765326434 29.444200227646768 21.505330010319557 30-31 22.371266920814193 26.905012149586987 29.58342253703678 21.140298392562038 32-33 22.138319741244974 27.03293648387244 29.164381256204674 21.66436251867791 34-35 22.065182634613738 27.09187855057556 29.55547351882002 21.287465295990682 36-37 21.78553113771537 26.909449925633393 29.538245561429544 21.766773375221693 38-39 21.719122938248518 26.954267757216076 29.554796428259316 21.771812876276087 40-41 21.674290305038074 26.355125351894625 30.08392672927605 21.886657613791254 42-43 21.319248648775694 26.7501194786473 29.47212068382322 22.45851118875378 44-45 20.76768691185641 27.118832875794872 29.90698714628348 22.20649306606524 46-47 20.743235405289713 26.577576443169466 29.65748152276691 23.02170662877391 48-49 20.89978077432939 26.711466757870177 29.6337887206851 22.75496374711533 50-51 20.844573193612437 26.90255767183168 29.362969261505196 22.889899873050688 52-53 20.60000044289024 27.135629514818856 29.07310397410523 23.19126606818568 54-55 20.21723574599075 26.851940014890502 29.452054444521476 23.47876979459728 56-57 20.691979759064054 26.567880543732446 28.859207633101526 23.880932064101977 58-59 20.243592645754546 26.811678997722517 29.037945994761667 23.906782361761277 60-61 20.85126364636909 26.859617443507805 28.58213675851279 23.706982151610315 62-63 20.757873968810014 26.683473641254547 28.41346111266668 24.145191277268754 64-65 20.33988243238363 26.45949354754525 28.30027780408713 24.90034621598399 66-67 20.563532867127623 26.558816057191958 27.892002398447246 24.985648677233172 68-69 20.3452615994626 26.7592224320521 27.81749435862096 25.078021609864344 70-71 20.485498451176753 26.690504066608856 27.68359463992479 25.140402842289593 72-73 20.654230767264778 27.08861600639288 27.054436593440485 25.20271663290186 74-75 20.807828908061065 27.213418395691935 26.777504189713568 25.20124850653343 76-77 20.585942870519013 27.20244359227418 26.817467752715253 25.394145784491556 78-79 20.49491723245483 27.438751539632538 26.73810482157296 25.328226406339667 80-81 20.476515867114177 27.584756833464873 26.45896344369228 25.47976385572867 82-83 20.771697869558093 27.155749147083867 26.614697245011893 25.457855738346147 84-85 20.83470360965715 27.4301772504254 26.17361824175457 25.56150089816288 86-87 20.483805364819556 27.615643894387134 26.366615371429265 25.53393536936405 88-89 20.460603350477736 27.16639863094728 26.42648712107824 25.946510897496744 90-91 20.921121857604717 26.713477747670634 26.379372502303468 25.986027892421177 92-93 20.875477777103907 26.717953243432756 26.354164608139296 26.05240437132404 94-95 20.449324140186405 26.505474328228047 26.6643558892462 26.380845642339345 96-97 20.40950742607985 26.51660648589726 26.733393514102737 26.340492573920155 98-99 20.339868906928555 26.698424607173937 26.563212304949822 26.39849418094769 100 20.250394531345822 26.82561033113377 26.71951615057333 26.204478986947077 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3112.0 1 2122.5 2 2253.0 3 3319.5 4 2334.5 5 1496.0 6 1976.0 7 3048.0 8 4387.5 9 5001.5 10 4128.5 11 3184.0 12 3138.5 13 3396.5 14 3695.5 15 3976.0 16 4321.0 17 4702.5 18 5213.0 19 5732.0 20 6276.0 21 6903.0 22 7691.0 23 8885.0 24 10349.5 25 12089.5 26 14563.0 27 17377.0 28 20489.0 29 24488.5 30 29321.0 31 33881.5 32 39469.0 33 45455.5 34 51245.0 35 56706.0 36 61931.5 37 69636.0 38 75226.5 39 77554.0 40 79354.0 41 81567.5 42 85323.0 43 90661.5 44 97233.0 45 105164.5 46 112931.5 47 119806.0 48 126696.5 49 131953.5 50 134480.5 51 135082.5 52 130983.5 53 122500.0 54 113372.0 55 101334.0 56 87842.5 57 74195.5 58 61507.0 59 50715.5 60 39565.0 61 29722.0 62 22733.5 63 17340.0 64 12509.0 65 8669.5 66 6168.5 67 4600.5 68 3507.5 69 2633.5 70 1942.0 71 1449.5 72 1071.5 73 780.5 74 586.5 75 419.0 76 299.5 77 189.5 78 108.0 79 66.0 80 47.0 81 31.0 82 17.0 83 12.0 84 6.5 85 4.5 86 4.0 87 4.0 88 3.5 89 2.0 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0487785270928611 2 0.0 3 0.0 4 0.01757662009770693 5 0.0042238389382086424 6 0.0 7 1.3625286897447236E-4 8 4.087586069234171E-4 9 0.0017372240794245222 10-11 7.49390779359598E-4 12-13 3.91726998301608E-4 14-15 1.7031608621809045E-5 16-17 8.515804310904522E-5 18-19 0.0 20-21 1.7031608621809045E-5 22-23 0.0 24-25 5.1094825865427134E-5 26-27 3.406321724361809E-5 28-29 0.0010048649086867336 30-31 7.323591707377888E-4 32-33 0.014715309849243014 34-35 0.024474421589539594 36-37 0.03178098168829567 38-39 0.02120435273415226 40-41 8.515804310904522E-5 42-43 0.00160097121045005 44-45 0.008975657743693367 46-47 0.014187329981966932 48-49 0.005399019933113466 50-51 0.010031617478245526 52-53 0.015447669019980802 54-55 0.009861301392027436 56-57 0.0028272470312203013 58-59 0.008294393398821003 60-61 0.00160097121045005 62-63 0.0010900229517957789 64-65 0.018939148787451655 66-67 0.04494641515295406 68-69 0.05433083150357085 70-71 0.04746709322898181 72-73 0.04097805034407256 74-75 0.0290048294829408 76-77 0.029243272003646126 78-79 0.012211663381837082 80-81 0.0018734769483989948 82-83 0.02680775197072743 84-85 0.04751818805484723 86-87 0.04760334609795627 88-89 0.058537638833157685 90-91 0.03454010228502874 92-93 0.02629680371207316 94-95 0.006744517014236381 96-97 0.004019459634746934 98-99 0.023980504939507135 100 0.020097298173734672 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2935718.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.918160432684 #Duplication Level Percentage of deduplicated Percentage of total 1 80.76692366705667 54.047739556064414 2 12.372175107204653 16.558463974503606 3 3.1934106221279337 6.410914930169831 4 1.2716022488183898 3.403731331719631 5 0.669161086110464 2.238951445782455 6 0.40720201028036657 1.634952567147181 7 0.28632528733093277 1.3412253059481993 8 0.19414483161614768 1.0393451991412634 9 0.14503063801271918 0.8734665151970695 >10 0.6534461504069685 7.372256790654376 >50 0.027046657657198072 1.2461841388439114 >100 0.012156768974508233 1.4389930109783584 >500 7.699576657219982E-4 0.3623251876160029 >1k 3.849788328399381E-4 0.5951039394108366 >5k 1.0999395223998232E-4 0.45367014601839106 >10k+ 1.0999395223998232E-4 0.982675960804499 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14368 0.4894203053563047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12333 0.4201016582655419 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7164 0.24402888833328 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5163 0.17586839062880016 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4284 0.14592682267165988 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3530 0.12024315686997185 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 3.406321724361808E-5 8 0.0 0.0 0.0 0.0 3.406321724361808E-5 9 0.0 0.0 0.0 0.0 3.406321724361808E-5 10-11 0.0 0.0 0.0 6.812643448723617E-5 3.406321724361808E-5 12-13 0.0 0.0 0.0 2.2141091208351755E-4 1.0218965173085425E-4 14-15 0.0 0.0 0.0 2.384425207053266E-4 2.043793034617085E-4 16-17 0.0 0.0 0.0 2.8953734657075373E-4 2.384425207053266E-4 18-19 0.0 0.0 0.0 4.7688504141065314E-4 2.384425207053266E-4 20-21 0.0 0.0 0.0 0.0014647183414755777 2.384425207053266E-4 22-23 0.0 0.0 0.0 0.004751818805484723 2.5547412932713564E-4 24-25 0.0 0.0 0.0 0.012058378904240802 2.7250573794894466E-4 26-27 0.0 0.0 0.0 0.02725057379489447 2.7250573794894466E-4 28-29 0.0 0.0 0.0 0.07585878480153747 3.0656895519256275E-4 30-31 0.0 0.0 0.0 0.2086372056171608 3.0656895519256275E-4 32-33 0.0 0.0 0.0 0.46726218253933105 3.0656895519256275E-4 34-35 0.0 0.0 0.0 0.8506436926162526 3.0656895519256275E-4 36-37 0.0 0.0 0.0 1.4168424896396725 3.0656895519256275E-4 38-39 0.0 0.0 0.0 2.190162679112912 3.0656895519256275E-4 40-41 0.0 0.0 0.0 3.0064025223131106 3.0656895519256275E-4 42-43 0.0 0.0 0.0 3.7661144564975246 3.2360056381437177E-4 44-45 0.0 0.0 0.0 4.649288521581433 3.4063217243618084E-4 46-47 0.0 0.0 0.0 5.621844468712594 3.7469538967979893E-4 48-49 0.0 0.0 0.0 6.606543952791106 4.08758606923417E-4 50-51 0.0 0.0 0.0 7.573547595511558 4.08758606923417E-4 52-53 0.0 0.0 0.0 8.490887067490814 4.428218241670351E-4 54-55 0.0 0.0 0.0 9.483421091535359 4.428218241670351E-4 56-57 0.0 0.0 0.0 10.553363776766023 4.598534327888441E-4 58-59 0.0 0.0 0.0 11.693306373432325 4.768850414106532E-4 60-61 0.0 0.0 0.0 12.803852413617385 4.768850414106532E-4 62-63 0.0 0.0 0.0 13.900398471515317 4.768850414106532E-4 64-65 0.0 0.0 0.0 14.969080817707969 4.768850414106532E-4 66-67 0.0 0.0 0.0 16.10919373046049 4.768850414106532E-4 68-69 3.406321724361808E-5 0.0 0.0 17.31579804327255 4.768850414106532E-4 70-71 3.406321724361808E-5 0.0 0.0 18.54742178915005 4.768850414106532E-4 72-73 3.406321724361808E-5 0.0 0.0 19.68286804114019 5.109482586542713E-4 74-75 3.406321724361808E-5 0.0 0.0 20.771903159635905 5.109482586542713E-4 76-77 3.406321724361808E-5 0.0 0.0 21.92194550021494 5.109482586542713E-4 78-79 3.406321724361808E-5 0.0 0.0 23.125978040125105 5.109482586542713E-4 80-81 3.406321724361808E-5 0.0 0.0 24.28542182866338 5.109482586542713E-4 82-83 3.406321724361808E-5 0.0 0.0 25.434527430768213 5.109482586542713E-4 84-85 3.406321724361808E-5 0.0 0.0 26.529540643890183 5.109482586542713E-4 86-87 3.406321724361808E-5 0.0 0.0 27.613840975189035 5.109482586542713E-4 88 3.406321724361808E-5 0.0 0.0 28.440844795038217 5.109482586542713E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5965 0.0 56.523945 1 TACCTGG 2640 0.0 48.60022 2 ACCTGGG 3095 0.0 40.84803 3 GTATCAA 10610 0.0 40.154716 1 ATCAACG 12835 0.0 32.66243 3 TATCAAC 13170 0.0 32.29552 2 CAACGCA 13030 0.0 32.167595 5 TCAACGC 13535 0.0 31.008982 4 AACGCAG 13820 0.0 30.632702 6 CCTGGGG 2995 0.0 30.600754 4 CTGGGGG 2005 0.0 28.123217 5 GTGGTAT 2275 0.0 28.111322 1 TGGGGGG 5665 0.0 27.121668 6 ACGCAGA 15430 0.0 27.071007 7 GTACATG 10205 0.0 26.864532 1 CGCAGAG 15600 0.0 26.655523 8 TACATGG 10290 0.0 26.079557 2 TAACGCA 325 0.0 24.584415 4 GTACCTG 5385 0.0 24.538294 1 ACATGGG 10600 0.0 24.252748 3 >>END_MODULE