FastQCFastQC Report
Mon 6 Jun 2016
ERR1211441_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211441_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764689
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136220.49271364699609976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115030.4160684981204034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66620.2409674288862147No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48330.17481170576509691No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39350.1423306563595399No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36310.13133484453405067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG21850.042.432132
GGTATCA75650.039.9210131
ACCTGGG26800.034.024873
GTATCAA117700.033.2241631
GTACATG85150.028.716741
TACATGG85700.027.7325972
ATCAACG138750.027.6430973
CAACGCA139600.027.3737745
TATCAAC141850.027.3055252
TCAACGC144500.026.6402174
ACATGGG85800.026.2956243
AACGCAG147400.026.2760016
CATGGGG65750.026.0926864
ATAACGC2750.025.6382333
CCTGGGG27500.024.6122594
ATGGGGG50350.024.551945
TAACGCA2501.6370905E-1124.441344
ACGCAGA161300.023.8955427
CGCAGAG162350.023.538768
GTACACG4100.022.9825691
Produced by FastQC (version 0.11.5)