FastQCFastQC Report
Mon 6 Jun 2016
ERR1211441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211441_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764689
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124530.45043041007505724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110450.3995024395148966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65090.2354333525398336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41330.14949240221956248No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38170.1380625451904355No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34430.12453480301039285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG23050.046.2787672
GGTATCA75850.038.9206541
ACCTGGG27750.037.256513
GTATCAA116350.032.362511
TAACGCA3000.031.3299084
CCTGGGG24650.029.550524
ATCAACG140350.026.552373
CAACGCA142100.026.3217285
GTACATG88900.026.2274481
CTGGGGG17000.025.9821035
TATCAAC143800.025.9470772
ATAACGC2900.025.927733
TCAACGC144750.025.9080474
TACATGG90000.025.4280642
AACGCAG150500.025.133156
TGGGGGG50500.024.6571036
ACATGGG90900.024.039843
CATGGGG66650.023.3387834
ACGCAGA164800.022.7527147
TATCACG1452.5381434E-522.6859442
Produced by FastQC (version 0.11.5)