FastQCFastQC Report
Mon 6 Jun 2016
ERR1211440_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211440_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2982875
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152730.5120227967983908No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124450.4172149352554163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72160.2419142605707581No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44710.14988894941960357No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37540.12585173699870092No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37000.12404140300884213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56800.057.403731
TACCTGG25600.042.0985452
GTATCAA105650.041.5650941
ATCAACG126200.034.3792533
CAACGCA126650.034.18235
TATCAAC128850.033.894962
TCAACGC130200.033.214754
GTGGTAT20500.032.6374931
ACCTGGG32250.032.211923
AACGCAG135250.032.1825566
ACGCAGA149950.028.588757
CGCAGAG151000.028.0753678
ATAACGC2900.027.5552943
TAACGCA2950.027.0882534
TGGTATC24650.026.3471552
GTACATG81850.026.1348191
GCAGAGT161350.025.6048989
TACATGG82800.024.952022
GTACTAG9550.024.6688541
CCTGGGG31250.023.315024
Produced by FastQC (version 0.11.5)