##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211440_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2982875 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42010644093366 31.0 31.0 34.0 30.0 34.0 2 31.458404726983197 31.0 31.0 34.0 28.0 34.0 3 31.527536353350374 31.0 31.0 34.0 28.0 34.0 4 35.16192163600553 37.0 35.0 37.0 32.0 37.0 5 35.220503038176254 37.0 35.0 37.0 32.0 37.0 6 35.288793864979255 37.0 35.0 37.0 32.0 37.0 7 35.22792876000503 37.0 35.0 37.0 32.0 37.0 8 35.2332920420735 37.0 35.0 37.0 32.0 37.0 9 36.81598156141307 39.0 37.0 39.0 32.0 39.0 10-11 36.75065867661233 39.0 37.0 39.0 32.0 39.0 12-13 36.66759351296987 39.0 37.0 39.0 32.0 39.0 14-15 37.85747374596656 40.0 37.5 41.0 32.0 41.0 16-17 37.85696215899091 40.0 37.5 41.0 32.0 41.0 18-19 37.87197535934291 40.0 37.5 41.0 32.0 41.0 20-21 37.855364371621334 40.0 37.5 41.0 32.0 41.0 22-23 37.71749268742404 40.0 37.0 41.0 32.0 41.0 24-25 37.61927955412144 40.0 37.0 41.0 32.0 41.0 26-27 37.4800658760424 40.0 37.0 41.0 32.0 41.0 28-29 37.34285177890458 40.0 36.0 41.0 31.0 41.0 30-31 37.16307119808909 39.5 36.0 41.0 31.0 41.0 32-33 36.93869303943343 39.0 35.5 41.0 30.5 41.0 34-35 36.78127628546285 39.0 35.0 41.0 30.0 41.0 36-37 36.617854418974986 39.0 35.0 41.0 30.0 41.0 38-39 36.445402505971586 39.0 35.0 41.0 30.0 41.0 40-41 36.264019109081005 39.0 35.0 40.0 29.0 41.0 42-43 36.036987973012614 39.0 35.0 40.0 28.0 41.0 44-45 35.874120102250345 38.5 35.0 40.0 27.5 41.0 46-47 35.60736403637431 38.0 34.5 40.0 26.5 41.0 48-49 35.3339794661191 38.0 34.0 40.0 26.0 41.0 50-51 34.660045761220296 37.5 33.5 39.5 24.5 40.5 52-53 34.942567154171726 38.0 34.0 39.5 25.5 40.5 54-55 35.33227356158069 38.0 34.0 40.0 26.0 41.0 56-57 35.244011566022714 38.0 34.0 40.0 26.0 41.0 58-59 35.03693751833382 38.0 34.0 40.0 25.0 41.0 60-61 34.81357029711269 38.0 34.0 40.0 25.0 41.0 62-63 34.53709072622889 37.0 33.5 40.0 24.5 41.0 64-65 34.32377421112182 37.0 33.0 40.0 24.0 41.0 66-67 34.11372032016092 36.0 33.0 40.0 23.5 41.0 68-69 33.76558974144072 36.0 33.0 39.0 23.0 41.0 70-71 33.38431630557767 35.0 32.5 39.0 22.5 41.0 72-73 32.99125541633491 35.0 32.0 38.5 21.5 40.0 74-75 32.603677324728665 35.0 32.0 37.0 20.5 40.0 76-77 32.21186405732725 35.0 31.0 37.0 20.0 39.0 78-79 31.80609328248753 35.0 31.0 36.0 19.5 39.0 80-81 31.396169970246824 35.0 31.0 36.0 18.0 38.5 82-83 31.023570883794996 35.0 30.0 35.5 18.0 37.0 84-85 30.684680383857852 34.0 30.0 35.0 16.5 37.0 86-87 30.347208146502958 34.0 29.0 35.0 14.5 36.5 88-89 30.015285756191595 34.0 29.0 35.0 11.5 36.0 90-91 29.689877886267446 34.0 29.0 35.0 10.5 36.0 92-93 29.380998868541255 34.0 28.5 35.0 10.0 35.0 94-95 29.08013845702552 34.0 27.0 35.0 10.0 35.0 96-97 28.77713782843733 34.0 26.5 35.0 10.0 35.0 98-99 28.440743410300463 34.0 25.0 35.0 8.0 35.0 100 27.179246867535515 32.0 23.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.27010016553719396 1101 2 -0.31338666037239804 1101 3 -0.3044560989362175 1101 4 -0.22256731228385007 1101 5 -0.1981431900495778 1101 6 -0.22047026444327145 1101 7 -0.26660269952983384 1101 8 -0.17814572263759487 1101 9 -0.32119717153988603 1101 10-11 -0.39489131788473486 1101 12-13 -0.4097024217596257 1101 14-15 -0.6159729956762376 1101 16-17 -0.5392622656278121 1101 18-19 -0.47634114408958084 1101 20-21 -0.4491736859228155 1101 22-23 -0.47200426377456495 1101 24-25 -0.5511960512929761 1101 26-27 -0.6415319958630974 1101 28-29 -0.6630091023945397 1101 30-31 -0.5638868473109611 1101 32-33 -0.6174915021485603 1101 34-35 -0.6963203084675627 1101 36-37 -0.8055418865747868 1101 38-39 -0.6739862541513943 1101 40-41 -0.8979151610257148 1101 42-43 -0.9681052668888697 1101 44-45 -0.9523585204294065 1101 46-47 -0.8846305618721573 1101 48-49 -0.9850196349195244 1101 50-51 -0.9213672176863454 1101 52-53 -0.689730103218956 1101 54-55 -0.6732636758556438 1101 56-57 -0.7230356529116264 1101 58-59 -0.5719520199519224 1101 60-61 -0.6032203751730449 1101 62-63 -0.6241487283116882 1101 64-65 -0.6270538350250803 1101 66-67 -0.7153746450177252 1101 68-69 -0.763746439998215 1101 70-71 -0.7136418250765786 1101 72-73 -0.763021974024511 1101 74-75 -0.8778175631281826 1101 76-77 -0.838203541496064 1101 78-79 -0.8140220313244377 1101 80-81 -1.0268418966704438 1101 82-83 -1.0860004685609148 1101 84-85 -1.0907789569309152 1101 86-87 -1.272655863226678 1101 88-89 -1.2914625966564905 1101 90-91 -1.3485569956482237 1101 92-93 -1.6287005658099893 1101 94-95 -1.8253354888921578 1101 96-97 -1.5886792820951143 1101 98-99 -1.720176423697918 1101 100 -1.7556540145642359 1102 1 -0.42869359788716466 1102 2 -0.49106321378090456 1102 3 -0.566267875533498 1102 4 -0.5031879069578977 1102 5 -0.4024033607935351 1102 6 -0.37817472050060985 1102 7 -0.4273036493868787 1102 8 -0.3521095381071717 1102 9 -0.5069140285000628 1102 10-11 -0.5382316829654812 1102 12-13 -0.6977349855524366 1102 14-15 -0.7732132051282505 1102 16-17 -0.7655310442679308 1102 18-19 -0.9031559662680877 1102 20-21 -0.974062629490497 1102 22-23 -0.9146800345628492 1102 24-25 -0.8801924543272577 1102 26-27 -0.9418357976067213 1102 28-29 -1.0210588529594276 1102 30-31 -1.0183252300934384 1102 32-33 -1.136462864423308 1102 34-35 -1.1156131779175595 1102 36-37 -0.9844012461722613 1102 38-39 -1.0515976509540437 1102 40-41 -1.2796844013654507 1102 42-43 -1.3197063503933677 1102 44-45 -1.1872918464356417 1102 46-47 -1.3672237453890332 1102 48-49 -1.3510461686776196 1102 50-51 -1.2780243056167322 1102 52-53 -1.0854486612589724 1102 54-55 -1.0555616093369338 1102 56-57 -1.086486409120333 1102 58-59 -0.9224485592886964 1102 60-61 -1.082249009193589 1102 62-63 -0.9856287325968154 1102 64-65 -1.1670556071118767 1102 66-67 -1.1850667125212695 1102 68-69 -1.145174769738496 1102 70-71 -1.277090481949017 1102 72-73 -1.0860103390706826 1102 74-75 -1.1290623460460054 1102 76-77 -1.1516368392538823 1102 78-79 -1.149770424637559 1102 80-81 -1.3215426316542462 1102 82-83 -1.4594649815967138 1102 84-85 -1.4076896662935532 1102 86-87 -1.45607587650807 1102 88-89 -1.6052092512252187 1102 90-91 -1.6615147011230782 1102 92-93 -1.6062259361344537 1102 94-95 -1.7295695568594027 1102 96-97 -2.0076894357819626 1102 98-99 -2.075639051035786 1102 100 -2.02368137015074 1103 1 -0.3418111779641855 1103 2 -0.3713182060068938 1103 3 -0.5058577223023271 1103 4 -0.34849428802885285 1103 5 -0.32425720705877836 1103 6 -0.30892131794938393 1103 7 -0.40363156273408407 1103 8 -0.30415405372922066 1103 9 -0.4973996225711872 1103 10-11 -0.5018526896058333 1103 12-13 -0.5823407482786251 1103 14-15 -0.6700873028274899 1103 16-17 -0.764415848555295 1103 18-19 -0.7975064294134313 1103 20-21 -0.791749113353923 1103 22-23 -0.6479266091077847 1103 24-25 -0.7814960822080081 1103 26-27 -0.8424751400328248 1103 28-29 -0.7774366139443387 1103 30-31 -0.7802233354576344 1103 32-33 -0.904973088848493 1103 34-35 -0.93643460740725 1103 36-37 -0.8892742539439027 1103 38-39 -0.6770890862450614 1103 40-41 -0.7508211207838613 1103 42-43 -0.9033877729498485 1103 44-45 -0.8845994770222987 1103 46-47 -0.9387415996029418 1103 48-49 -0.9365283508143349 1103 50-51 -0.8231746597693288 1103 52-53 -0.5946339906207285 1103 54-55 -0.6364506096091205 1103 56-57 -0.6820790143989726 1103 58-59 -0.7151656382590943 1103 60-61 -0.5780439367154742 1103 62-63 -0.6335889898410016 1103 64-65 -0.7402328081198846 1103 66-67 -0.8436950646470862 1103 68-69 -0.7562149117600754 1103 70-71 -0.7417490328713683 1103 72-73 -0.6144175375577845 1103 74-75 -0.7497631830855767 1103 76-77 -0.9350576899116376 1103 78-79 -0.8986496876715471 1103 80-81 -0.9147689000914738 1103 82-83 -0.9749713295088149 1103 84-85 -1.138509910693859 1103 86-87 -1.1885414298605852 1103 88-89 -1.2276132479022372 1103 90-91 -1.1601177880888365 1103 92-93 -1.1889943817848057 1103 94-95 -1.2577780090587787 1103 96-97 -1.140933897262272 1103 98-99 -1.3973628182251225 1103 100 -1.2816961651494871 1104 1 -0.4815940006529509 1104 2 -0.5541968362502523 1104 3 -0.5274338489334376 1104 4 -0.37754141725338997 1104 5 -0.3208623409009306 1104 6 -0.2838011572233867 1104 7 -0.3217337431911531 1104 8 -0.3398616363906726 1104 9 -0.5256356423840529 1104 10-11 -0.5370399352850015 1104 12-13 -0.5416423425967452 1104 14-15 -0.7093779273495286 1104 16-17 -0.6638277066919613 1104 18-19 -0.7722130097783904 1104 20-21 -0.7963092402181005 1104 22-23 -0.7581245976796964 1104 24-25 -0.8103901917412486 1104 26-27 -0.776517560312719 1104 28-29 -1.0397171970130827 1104 30-31 -0.9095604107352031 1104 32-33 -0.9345367033010632 1104 34-35 -0.780561606607769 1104 36-37 -0.8744333047820163 1104 38-39 -0.9373299203305336 1104 40-41 -1.1355016997067295 1104 42-43 -1.1012561001007626 1104 44-45 -1.1219924052575365 1104 46-47 -0.9590726291670464 1104 48-49 -0.852269195824789 1104 50-51 -0.9572257920169918 1104 52-53 -0.7760442621147732 1104 54-55 -0.7727801595393444 1104 56-57 -0.7166762413600196 1104 58-59 -0.8146181429078041 1104 60-61 -0.8371338666331596 1104 62-63 -0.8645506090008297 1104 64-65 -0.8985330443794837 1104 66-67 -0.9151169530319336 1104 68-69 -0.8813907618492394 1104 70-71 -0.779053552841944 1104 72-73 -0.7429594706958156 1104 74-75 -0.8479587552769772 1104 76-77 -1.0141470903881888 1104 78-79 -0.8753943362542991 1104 80-81 -0.9401101515110213 1104 82-83 -0.9973634677842718 1104 84-85 -1.04483846897244 1104 86-87 -1.1022927387567094 1104 88-89 -1.0379256966038746 1104 90-91 -1.0283827506834022 1104 92-93 -1.0849753773694317 1104 94-95 -1.3153001769549242 1104 96-97 -1.3957423572272774 1104 98-99 -1.4667239480580925 1104 100 -1.4054489671256185 1105 1 -0.6062208155239546 1105 2 -0.5740538508340514 1105 3 -0.5481968808336752 1105 4 -0.459080544559356 1105 5 -0.48527287857925927 1105 6 -0.4178268887634857 1105 7 -0.42067115830268165 1105 8 -0.44580569346813803 1105 9 -0.5972758416754473 1105 10-11 -0.6482247919916304 1105 12-13 -0.7023295380922647 1105 14-15 -0.8242561417660639 1105 16-17 -0.6915888714509748 1105 18-19 -0.8329364297587531 1105 20-21 -0.8429590021251769 1105 22-23 -0.8533617667564712 1105 24-25 -0.8822309628446448 1105 26-27 -0.9510506939656764 1105 28-29 -0.8244153100983098 1105 30-31 -0.8262736748935495 1105 32-33 -0.9618254100140149 1105 34-35 -0.9581559928661321 1105 36-37 -0.9679714887926778 1105 38-39 -0.9436343583566824 1105 40-41 -1.0915088052164137 1105 42-43 -0.8558919427292793 1105 44-45 -0.9695647164607024 1105 46-47 -0.8939021876983588 1105 48-49 -1.0113651882784254 1105 50-51 -1.0799206343634964 1105 52-53 -0.9260051202217667 1105 54-55 -0.7846645458949482 1105 56-57 -0.777030528167181 1105 58-59 -0.8003872689495637 1105 60-61 -0.7603642964970376 1105 62-63 -0.8370260784604326 1105 64-65 -0.8816501653514308 1105 66-67 -0.9774765527635267 1105 68-69 -1.0415580914158724 1105 70-71 -0.9769766013389898 1105 72-73 -0.7674636733843698 1105 74-75 -0.854435806620117 1105 76-77 -0.8962035714104495 1105 78-79 -0.912397606993018 1105 80-81 -0.9198621231927149 1105 82-83 -0.9087001917942139 1105 84-85 -0.9095647350465477 1105 86-87 -1.0149921355015294 1105 88-89 -1.0663858237380417 1105 90-91 -0.996499531291068 1105 92-93 -1.0008912166267265 1105 94-95 -1.3238137412821551 1105 96-97 -1.185669924465433 1105 98-99 -1.2601390336516083 1105 100 -1.4357105938075847 1106 1 -0.527254581130876 1106 2 -0.5927312876669788 1106 3 -0.5580076221343759 1106 4 -0.3752935250703686 1106 5 -0.43167048037176414 1106 6 -0.42080571989759363 1106 7 -0.5423844475805453 1106 8 -0.5817518331727172 1106 9 -0.6299672712068087 1106 10-11 -0.7065627982332217 1106 12-13 -0.5670630403601038 1106 14-15 -0.8143306480348542 1106 16-17 -0.727534183045222 1106 18-19 -0.871148989116513 1106 20-21 -0.9879220802349096 1106 22-23 -0.824332950883246 1106 24-25 -0.8071603960149645 1106 26-27 -0.7762448858364692 1106 28-29 -0.9042693970593589 1106 30-31 -0.8439715755155817 1106 32-33 -0.8690190860903826 1106 34-35 -0.8294114554367198 1106 36-37 -0.8210357335915504 1106 38-39 -0.917343038609097 1106 40-41 -0.8955619320426891 1106 42-43 -0.8346420403279993 1106 44-45 -0.7463156374376823 1106 46-47 -0.598645958534199 1106 48-49 -0.6882690730622514 1106 50-51 -0.8169766785041475 1106 52-53 -0.7148009058500335 1106 54-55 -0.5845800786419773 1106 56-57 -0.6174311916018453 1106 58-59 -0.6195463110861823 1106 60-61 -0.6776112318973375 1106 62-63 -0.6126377562356069 1106 64-65 -0.6432975370475731 1106 66-67 -0.5690407112023266 1106 68-69 -0.5242328404393035 1106 70-71 -0.7024420584913713 1106 72-73 -0.6242455420271398 1106 74-75 -0.6390242002990334 1106 76-77 -0.7757158854109569 1106 78-79 -0.7405475612103452 1106 80-81 -0.7616273941385785 1106 82-83 -0.8380511425831649 1106 84-85 -0.8696598730925409 1106 86-87 -0.8385514009913351 1106 88-89 -0.8159954288713784 1106 90-91 -0.8483121464871282 1106 92-93 -0.9363542131084408 1106 94-95 -0.8561189164984704 1106 96-97 -0.9557498252814796 1106 98-99 -1.1499938540817993 1106 100 -1.1124376178866662 1107 1 -0.4702086381123074 1107 2 -0.5964560302115522 1107 3 -0.5980211584214743 1107 4 -0.5149383597582755 1107 5 -0.4718926444950924 1107 6 -0.4247734214413299 1107 7 -0.42987104050688885 1107 8 -0.4582562266184098 1107 9 -0.7674511289580863 1107 10-11 -0.7213382139515332 1107 12-13 -0.7396071266663853 1107 14-15 -0.7831511613196938 1107 16-17 -0.9236413309819795 1107 18-19 -0.8935076305531737 1107 20-21 -0.8356496024718822 1107 22-23 -0.9525402265998366 1107 24-25 -1.0254965946799643 1107 26-27 -0.9273691559691528 1107 28-29 -1.059504352370375 1107 30-31 -0.904995217614335 1107 32-33 -1.1928687186148963 1107 34-35 -1.165045919849831 1107 36-37 -1.0104356663628309 1107 38-39 -0.9225845772035441 1107 40-41 -0.9932691762576056 1107 42-43 -1.0757530043055539 1107 44-45 -1.03296387091072 1107 46-47 -1.156221016453891 1107 48-49 -1.0590637413949295 1107 50-51 -1.0985671016942078 1107 52-53 -1.0067463245827 1107 54-55 -0.9743344146489932 1107 56-57 -1.014603888292065 1107 58-59 -0.9945725781984791 1107 60-61 -0.8516055268880436 1107 62-63 -0.8348348903003071 1107 64-65 -0.7529186379611943 1107 66-67 -0.7989180282257209 1107 68-69 -0.8523416121268781 1107 70-71 -0.6783427139147733 1107 72-73 -0.7776313303592985 1107 74-75 -0.7963782560732149 1107 76-77 -0.8345006452418353 1107 78-79 -0.8294737616598198 1107 80-81 -0.8433715907573003 1107 82-83 -0.8210143972981392 1107 84-85 -0.9731711588021348 1107 86-87 -0.9202333487981598 1107 88-89 -0.9294550178858607 1107 90-91 -0.9678732508640486 1107 92-93 -1.0025377158151336 1107 94-95 -1.0281733121511643 1107 96-97 -1.0406909699729923 1107 98-99 -0.937317770113058 1107 100 -1.2069637591809048 1108 1 -0.5152191511162911 1108 2 -0.598382450008323 1108 3 -0.49788502873732554 1108 4 -0.4763718908099932 1108 5 -0.515211148016661 1108 6 -0.4220358971651166 1108 7 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1.1478760467463758 2311 58-59 1.103735209893074 2311 60-61 1.0966248176680367 2311 62-63 1.0880595315201091 2311 64-65 1.1248401439172397 2311 66-67 1.0677889387458706 2311 68-69 1.0668734650179843 2311 70-71 1.1147590184471028 2311 72-73 1.0732757822987438 2311 74-75 1.0947825570768899 2311 76-77 1.0845363829308923 2311 78-79 1.1572562529569304 2311 80-81 1.1730303627401 2311 82-83 1.2292602080374984 2311 84-85 1.1653400170406947 2311 86-87 1.2953897193730022 2311 88-89 1.2754970887947898 2311 90-91 1.3880955540339706 2311 92-93 1.4054332154476405 2311 94-95 1.449374076036289 2311 96-97 1.521979010920191 2311 98-99 1.6433622176034213 2311 100 1.8777641992282952 2312 1 0.5525806564727951 2312 2 0.6124104948203879 2312 3 0.7237052316681201 2312 4 0.5680928263266622 2312 5 0.5892339517133252 2312 6 0.42551182410228705 2312 7 0.6140024476956327 2312 8 0.603493937104659 2312 9 0.7891249369593467 2312 10-11 0.7918570699263583 2312 12-13 0.8506183110433696 2312 14-15 1.0508348686211093 2312 16-17 1.025740210072911 2312 18-19 1.0796880905050728 2312 20-21 1.1287738703268602 2312 22-23 1.0704787828412066 2312 24-25 1.1385941721587685 2312 26-27 1.0666796288695153 2312 28-29 1.113261419374446 2312 30-31 1.1482361746284653 2312 32-33 1.1378795419098537 2312 34-35 1.1698801942306218 2312 36-37 1.2322148976242175 2312 38-39 1.3279927594644576 2312 40-41 1.2762817390058174 2312 42-43 1.3454907113488304 2312 44-45 1.362203207613021 2312 46-47 1.2922133655560586 2312 48-49 1.3165196089425777 2312 50-51 1.2788838772628779 2312 52-53 1.1872751950347507 2312 54-55 1.154558161648005 2312 56-57 1.0976859345689007 2312 58-59 1.1221708731471978 2312 60-61 1.1712291446758272 2312 62-63 1.0915436452372305 2312 64-65 0.9817548971211139 2312 66-67 1.0364894233767998 2312 68-69 1.1141563204882132 2312 70-71 1.1596364413649525 2312 72-73 1.191031816029664 2312 74-75 1.1846807586881596 2312 76-77 1.1542875327768343 2312 78-79 1.2189189320806015 2312 80-81 1.2384710112386799 2312 82-83 1.317726154409808 2312 84-85 1.3004323110046556 2312 86-87 1.3456592061277455 2312 88-89 1.491398504543593 2312 90-91 1.411501028898396 2312 92-93 1.4960716017668254 2312 94-95 1.471341690892995 2312 96-97 1.64089574489671 2312 98-99 1.5181639982463473 2312 100 1.6245724018310561 2313 1 0.625937658758577 2313 2 0.6750284360695318 2313 3 0.7697718058422396 2313 4 0.6411986487052843 2313 5 0.6341738003229196 2313 6 0.649264513142235 2313 7 0.6907388053375882 2313 8 0.7079627517079743 2313 9 0.8357147030506695 2313 10-11 0.7444591078436176 2313 12-13 0.7949934229972584 2313 14-15 1.1360237213404574 2313 16-17 1.1440474396862186 2313 18-19 1.1486392523652071 2313 20-21 1.075215056484403 2313 22-23 1.1065052914307927 2313 24-25 1.2216961932386141 2313 26-27 1.2193018866199736 2313 28-29 1.24516327612708 2313 30-31 1.1882158174079223 2313 32-33 1.255755382726008 2313 34-35 1.2678190779181264 2313 36-37 1.313203804268106 2313 38-39 1.3300402415762917 2313 40-41 1.2508486160667616 2313 42-43 1.353996518689506 2313 44-45 1.2788660600386237 2313 46-47 1.295717116193245 2313 48-49 1.3543362223728863 2313 50-51 1.3697448113280828 2313 52-53 1.253719747182238 2313 54-55 1.2622967203995046 2313 56-57 1.2073734522789863 2313 58-59 1.2283372054673265 2313 60-61 1.1577518941161813 2313 62-63 1.2673173506583097 2313 64-65 1.2589810555820407 2313 66-67 1.236780199212852 2313 68-69 1.3455200444498558 2313 70-71 1.349160044025517 2313 72-73 1.332195222522003 2313 74-75 1.3021657922409133 2313 76-77 1.3251917528158046 2313 78-79 1.3992293319068985 2313 80-81 1.3932337115600717 2313 82-83 1.473709810094423 2313 84-85 1.5487024811282701 2313 86-87 1.5697861636827497 2313 88-89 1.6171701230210225 2313 90-91 1.567323963789267 2313 92-93 1.7286389210059632 2313 94-95 1.7984142181951874 2313 96-97 1.829025276672425 2313 98-99 1.951589114788522 2313 100 1.9638094585431354 2314 1 0.5024672063978777 2314 2 0.5065405899579432 2314 3 0.5992311703433941 2314 4 0.4838156171604666 2314 5 0.467845690657839 2314 6 0.52401718901055 2314 7 0.6101786867799674 2314 8 0.5315963273169757 2314 9 0.6750205876532007 2314 10-11 0.6450081969423351 2314 12-13 0.3118041095299944 2314 14-15 0.7453711479195988 2314 16-17 0.8167418338995986 2314 18-19 0.8643636074784311 2314 20-21 0.9345666674759201 2314 22-23 0.7974149580465237 2314 24-25 0.8447920048338347 2314 26-27 0.8877452801429087 2314 28-29 0.8899216275801862 2314 30-31 0.9043919441433843 2314 32-33 0.9293324524649762 2314 34-35 1.001279979674571 2314 36-37 1.0240568150270732 2314 38-39 1.0675581756779238 2314 40-41 1.1177859689285086 2314 42-43 1.1211238982162897 2314 44-45 1.1539976878851732 2314 46-47 1.1373614533138507 2314 48-49 1.0664128567518318 2314 50-51 1.1437153534996796 2314 52-53 1.0550676134326196 2314 54-55 0.9996012313554488 2314 56-57 0.9895003742375579 2314 58-59 1.0520862418750454 2314 60-61 1.0559441627567523 2314 62-63 1.0323872310696132 2314 64-65 0.9387258767105493 2314 66-67 0.9356111577200679 2314 68-69 0.9527253779489726 2314 70-71 0.9774777848978644 2314 72-73 0.94615836389913 2314 74-75 1.0302536549017631 2314 76-77 1.052461680599734 2314 78-79 1.0952524953646794 2314 80-81 1.1998816885760206 2314 82-83 1.0790253470313367 2314 84-85 1.105740369297731 2314 86-87 1.212183166092899 2314 88-89 1.3333254544949575 2314 90-91 1.4020055473969748 2314 92-93 1.4306974924903244 2314 94-95 1.5260095157416877 2314 96-97 1.5285454133328003 2314 98-99 1.6030539743107362 2314 100 1.5742543604833337 2315 1 0.5072304728922283 2315 2 0.5168629635532866 2315 3 0.5719724518513942 2315 4 0.5450001698147986 2315 5 0.41994752469062746 2315 6 -0.2923517919020071 2315 7 0.2967329037893265 2315 8 0.2714464705013029 2315 9 0.5039364241231183 2315 10-11 0.6377712882516491 2315 12-13 0.6833553472895773 2315 14-15 0.8587477769840461 2315 16-17 0.6825225647966136 2315 18-19 0.7762623151943373 2315 20-21 0.771851417139807 2315 22-23 0.7142719544254206 2315 24-25 0.7713040154980462 2315 26-27 0.8260708718432639 2315 28-29 0.8507806525723964 2315 30-31 0.9860580569161783 2315 32-33 0.898451268037455 2315 34-35 0.9120118298972528 2315 36-37 0.9600603584757295 2315 38-39 1.0036010253496457 2315 40-41 1.0500581814529895 2315 42-43 1.1398430821189933 2315 44-45 1.1172978575128738 2315 46-47 1.235817248718341 2315 48-49 1.2730945466397188 2315 50-51 1.1457904129282568 2315 52-53 0.987141013019766 2315 54-55 0.9100716657517793 2315 56-57 0.9368799777673118 2315 58-59 0.9799268249615238 2315 60-61 0.8030543272797956 2315 62-63 0.9237226011130488 2315 64-65 1.0490995912995231 2315 66-67 1.0479176122950733 2315 68-69 1.095385364029859 2315 70-71 0.9929009138130738 2315 72-73 1.12892177539959 2315 74-75 1.1669708565636583 2315 76-77 1.1573932130603097 2315 78-79 1.0441096522071867 2315 80-81 1.0044504849554272 2315 82-83 1.1962436441517887 2315 84-85 1.1989370803826382 2315 86-87 1.291687657417743 2315 88-89 1.4329660675395424 2315 90-91 1.4725453123766776 2315 92-93 1.6567511513734026 2315 94-95 1.8042719598600492 2315 96-97 1.836634840142807 2315 98-99 1.8030458039160493 2315 100 1.8299261049398865 2316 1 0.04371522036734987 2316 2 -0.04216779223301259 2316 3 0.043808101424961166 2316 4 0.13021335118212107 2316 5 0.08604204499106771 2316 6 0.10901066620102284 2316 7 0.11973650628341659 2316 8 0.13725264378237512 2316 9 0.24318775431732575 2316 10-11 0.15139826602827355 2316 12-13 0.2919303525439787 2316 14-15 0.3998152877970611 2316 16-17 0.4558253918510786 2316 18-19 0.33603532488087495 2316 20-21 0.4078208641551271 2316 22-23 0.2337739105391492 2316 24-25 0.3799750749705382 2316 26-27 0.33595874098828205 2316 28-29 0.41253521837414553 2316 30-31 0.4378735000745735 2316 32-33 0.6021800522233534 2316 34-35 0.5501086658233802 2316 36-37 0.6921645911297318 2316 38-39 0.6905481605003345 2316 40-41 0.6097961617701699 2316 42-43 0.587235237752644 2316 44-45 0.6012654682517748 2316 46-47 0.3856985175927079 2316 48-49 0.5236511448823506 2316 50-51 0.3718306930157169 2316 52-53 0.23803039027132655 2316 54-55 0.08721703026002814 2316 56-57 0.1577779464326241 2316 58-59 0.3073044894981578 2316 60-61 0.35493909171650984 2316 62-63 0.37595655305275244 2316 64-65 0.35181565798069414 2316 66-67 0.41176914676581333 2316 68-69 0.3666826675058772 2316 70-71 0.34280080356274567 2316 72-73 0.41512628791982564 2316 74-75 0.5904132480002673 2316 76-77 0.5878071864601111 2316 78-79 0.639333489877405 2316 80-81 0.8216749789654045 2316 82-83 0.8268517540038864 2316 84-85 0.8258905208198009 2316 86-87 0.9331036182733463 2316 88-89 1.0077463965206341 2316 90-91 0.9910076336807982 2316 92-93 1.0778198421230663 2316 94-95 1.1464657570805592 2316 96-97 1.3242847750619262 2316 98-99 1.4158084780949771 2316 100 1.2627265002186618 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 11.0 1 156.0 2 78.0 3 61.0 4 46.0 5 36.0 6 65.0 7 286.0 8 983.0 9 1924.0 10 2717.0 11 3397.0 12 4170.0 13 4679.0 14 5643.0 15 7117.0 16 10095.0 17 13984.0 18 18145.0 19 22268.0 20 27172.0 21 32346.0 22 34898.0 23 35285.0 24 34643.0 25 35320.0 26 38394.0 27 44509.0 28 52042.0 29 61191.0 30 73555.0 31 89917.0 32 112361.0 33 143753.0 34 194534.0 35 269889.0 36 386159.0 37 550255.0 38 527417.0 39 143177.0 40 197.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.62650883756717 18.837281580799775 12.10120908603713 23.43500049559592 2 16.414372539368426 21.110783567832684 39.32810278800511 23.146741104793776 3 19.257057760140945 26.489373581347376 30.23057579733769 24.022992861173993 4 12.610631487245314 17.084177509340424 38.56869999912816 31.736491004286098 5 12.770802733680744 37.348745498078515 35.04275567896018 14.837696089280564 6 29.26955175949651 37.414747160530766 18.775968300853428 14.539732779119296 7 25.85266364090746 33.21398021515765 23.19127024860201 17.742085895332885 8 25.331834660269465 34.50654478711329 21.420693763534345 18.7409267890829 9 26.388613594905657 15.809577279241335 21.269285810828258 36.53252331502475 10-11 24.393365900907753 25.990061361442223 29.118923073233777 20.49764966441625 12-13 25.093576496112973 24.5294595964686 28.726965575956815 21.64999833146161 14-15 22.502176204859676 25.790425100997368 27.0124865202719 24.694912173871057 16-17 21.438936125801916 28.316010337632857 27.844244766845804 22.400808769719422 18-19 21.015860827753976 27.439290692985978 30.239350042206688 21.30549843705336 20-21 21.936374508157268 26.922007142523025 30.12569747402951 21.015920875290192 22-23 21.883733653099572 26.881288293105193 29.775766272877362 21.459211780917876 24-25 21.49173390980609 26.818993355574744 30.189072132696033 21.50020060192313 26-27 21.448916290324448 26.981813353371386 29.920023820337516 21.649246535966647 28-29 21.240739219437117 27.397827541171598 29.90628148749218 21.4551517518991 30-31 21.77952567396699 27.120329106394593 29.8540862848082 21.246058934830213 32-33 21.63832689613453 27.305252660332524 29.539348509429114 21.51707193410383 34-35 21.827276525625436 27.15124705162778 29.76757739539943 21.253899027347348 36-37 21.701070388946096 26.9109381806037 29.74726266327363 21.640728767176572 38-39 21.554090735182527 26.981539708923396 29.835318363149298 21.62905119274478 40-41 21.5772031615554 26.54036294645267 30.16082406194347 21.721609830048457 42-43 21.2393226595699 26.727992069794393 29.856714383826244 22.17597088680946 44-45 21.026848372345725 27.100808419231022 29.931432768395688 21.940910440027565 46-47 21.067104736676704 26.790965007279738 29.71354286325662 22.428387392786938 48-49 21.07565032434887 26.855455976625148 29.670610323221986 22.398283375803995 50-51 20.937247496108746 27.09021453158165 29.425905392288453 22.54663258002115 52-53 20.733910706787213 27.311114897362042 29.241001654174276 22.71397274167647 54-55 20.572626300247634 27.16730019212868 29.437829511078885 22.822243996544803 56-57 20.928561426574625 27.00133068482055 29.149694915140543 22.920412973464284 58-59 20.784852544781266 26.93238507451623 29.180491667769314 23.10227071293319 60-61 20.826916683111733 26.92942559404698 29.05426805253457 23.189389670306713 62-63 20.94990140368756 26.637586580908586 29.011308940868872 23.40120307453499 64-65 20.707659150876758 26.505554224465012 29.057548213934513 23.729238410723717 66-67 20.844151935342754 26.504729556049007 28.702778845781413 23.94833966282683 68-69 20.653432633299204 26.543200146756647 28.6978332266307 24.10553399331345 70-71 20.550437607993388 26.2636903420372 28.83996989600438 24.345902153965035 72-73 20.62503731300733 26.22294429645947 28.803897590971626 24.348120799561567 74-75 20.62587113541986 26.21040981362292 28.68676917051225 24.476949880444966 76-77 20.64262612582631 25.960786430784598 28.82992191118997 24.56666553219912 78-79 20.57814795025439 26.021771207009543 28.92429703116704 24.475783811569027 80-81 20.646845786812566 25.98346436243671 28.864416859154346 24.505272991596378 82-83 20.842875853461766 25.627559714659924 29.12191601956365 24.40764841231466 84-85 20.93819645874148 25.61737656911201 28.80128375566932 24.64314321647719 86-87 20.847849378813002 25.566563748805315 29.063122228497768 24.522464643883914 88-89 21.26353696800418 25.058020666640402 29.056471548790924 24.621970816564495 90-91 21.57813353210599 25.004514131116522 28.931615398376053 24.485736938401434 92-93 21.754010239737546 24.836879418355544 28.800041444480996 24.609068897425914 94-95 21.460340794471747 24.510161598386162 29.07085957786982 24.95863802927227 96-97 21.65311226917481 24.257783145959284 28.994584835729288 25.094519749136623 98-99 21.77393189858446 24.274746366417133 28.82952882260396 25.121792912394447 100 22.161546388675664 24.01461853870281 28.839856376680224 24.983978695941303 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2174.0 1 1506.5 2 2092.0 3 3233.0 4 2149.5 5 1351.5 6 1875.0 7 2936.0 8 4374.5 9 5006.5 10 4145.0 11 3265.5 12 3225.0 13 3450.0 14 3782.0 15 4165.0 16 4592.5 17 5055.0 18 5463.0 19 5892.0 20 6475.0 21 7267.5 22 8337.0 23 9817.5 24 11539.0 25 13709.5 26 16525.5 27 19673.0 28 23171.5 29 27679.5 30 33244.0 31 38306.5 32 44253.0 33 50465.0 34 56392.0 35 62559.5 36 68840.5 37 77039.0 38 82361.5 39 84414.5 40 86328.5 41 88634.0 42 91894.0 43 96358.0 44 102586.5 45 109659.0 46 115678.0 47 120476.0 48 124513.0 49 126027.0 50 125169.0 51 122898.5 52 117909.0 53 111380.5 54 104368.0 55 94228.0 56 83281.5 57 71431.5 58 59586.0 59 50341.0 60 40872.5 61 31739.0 62 24829.5 63 19134.0 64 13917.5 65 9922.0 66 7243.5 67 5376.0 68 4018.0 69 3026.5 70 2298.5 71 1741.0 72 1335.5 73 1026.5 74 808.0 75 626.0 76 437.5 77 294.5 78 188.0 79 138.5 80 100.0 81 69.5 82 50.5 83 36.0 84 36.5 85 30.5 86 24.0 87 22.5 88 14.5 89 9.5 90 12.0 91 8.0 92 4.5 93 4.0 94 3.5 95 3.0 96 3.5 97 3.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22327452541591586 2 0.14995599882663538 3 0.0245400829736412 4 0.022696224280266523 5 0.027322633365461174 6 0.05350542681138164 7 0.06051208984620542 8 0.052667309223484046 9 0.04069899006830659 10-11 0.042894858148598246 12-13 0.04101747475170767 14-15 0.020751791476344133 16-17 0.01902526924527511 18-19 0.01768428110463898 20-21 0.019963960943720403 22-23 0.023349955998826638 24-25 0.02013158446129992 26-27 0.018421824581988853 28-29 0.018103339898587773 30-31 0.01662825294388803 32-33 0.02443950886309349 34-35 0.024171311234966266 36-37 0.023064996018941457 38-39 0.02132171143611449 40-41 0.022528600762687007 42-43 0.02879772032016092 44-45 0.03788291497297071 46-47 0.043984411012865106 48-49 0.04053136655072707 50-51 0.029049155596530193 52-53 0.04448728156560366 54-55 0.042894858148598246 56-57 0.0445040439173616 58-59 0.04728659430918158 60-61 0.024288647697271928 62-63 0.025663160541423962 64-65 0.025998407576582998 66-67 0.03136236013912752 68-69 0.035804383354984703 70-71 0.04059841595775887 72-73 0.0449566274148263 74-75 0.025998407576582998 76-77 0.018958219838243306 78-79 0.018153626953861628 80-81 0.02177429493357918 82-83 0.020416544441185097 84-85 0.012605288521979634 86-87 0.01339311905460336 88-89 0.017851904622218497 90-91 0.01922641746637053 92-93 0.019176130411096677 94-95 0.022360977245107487 96-97 0.02326614424003688 98-99 0.029049155596530193 100 0.030440430792440177 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2982875.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.84377857983615 #Duplication Level Percentage of deduplicated Percentage of total 1 82.00031421706574 58.09212103870796 2 12.00082264794235 17.003672448934218 3 2.9179949084107926 6.201653555656304 4 1.1329086623967233 3.2103812172004726 5 0.5689294067969786 2.0152554461341334 6 0.35377493242378216 1.5037651787836153 7 0.23846611225340594 1.1825688318692251 8 0.15521845760921896 0.8797009633896954 9 0.10917819968638612 0.6961136583894728 >10 0.5028952784956155 5.756660676498498 >50 0.013166177820336815 0.6266938830542013 >100 0.005415952912873467 0.7293077400126708 >500 5.083589921455917E-4 0.24453554805381716 >1k 2.5417949605743904E-4 0.599454226383595 >5k 5.0835899211487806E-5 0.2598775426605 >10k+ 1.0167179842297561E-4 0.9982380442715824 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15273 0.5120227967983908 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12445 0.4172149352554163 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7216 0.2419142605707581 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4471 0.14988894941960357 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3754 0.12585173699870092 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3700 0.12404140300884213 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.352470351590328E-5 7 0.0 0.0 0.0 0.0 3.352470351590328E-5 8 0.0 0.0 0.0 0.0 3.352470351590328E-5 9 0.0 0.0 0.0 0.0 3.352470351590328E-5 10-11 0.0 0.0 0.0 1.676235175795164E-5 6.704940703180656E-5 12-13 0.0 0.0 0.0 5.0287055273854925E-5 1.676235175795164E-4 14-15 0.0 0.0 0.0 6.704940703180656E-5 2.011482210954197E-4 16-17 0.0 0.0 0.0 1.1733646230566147E-4 3.0172233164312955E-4 18-19 0.0 0.0 0.0 3.687717386749361E-4 3.0172233164312955E-4 20-21 0.0 0.0 0.0 9.889787537191467E-4 3.352470351590328E-4 22-23 0.0 0.0 0.0 0.003536856220927796 3.6877173867493605E-4 24-25 0.0 0.0 0.0 0.009655114612580146 4.0229644219083935E-4 26-27 0.0 0.0 0.0 0.02135523613963039 4.6934584922264595E-4 28-29 0.0 0.0 0.0 0.05935548757490676 4.6934584922264595E-4 30-31 0.0 0.0 0.0 0.16531031303691907 4.6934584922264595E-4 32-33 0.0 0.0 0.0 0.3946528097892134 4.6934584922264595E-4 34-35 0.0 0.0 0.0 0.7411306206260738 4.6934584922264595E-4 36-37 0.0 0.0 0.0 1.2662951012026986 4.861082009805976E-4 38-39 0.0 0.0 0.0 1.984360725809831 5.699199597703557E-4 40-41 0.0 0.0 0.0 2.709600636969367 5.699199597703557E-4 42-43 0.0 0.0 0.0 3.399991618824121 5.699199597703557E-4 44-45 0.0 0.0 0.0 4.206780371286091 5.699199597703557E-4 46-47 0.0 0.0 0.0 5.136940032686586 5.699199597703557E-4 48-49 0.0 0.0 0.0 6.074273980639484 6.034446632862591E-4 50-51 0.0 0.0 0.0 6.993571638100826 6.704940703180656E-4 52-53 0.0 0.0 0.0 7.841428152369778 6.704940703180656E-4 54-55 0.0 0.0 0.0 8.751120982273813 6.704940703180656E-4 56-57 0.0 0.0 0.0 9.771495620835603 6.704940703180656E-4 58-59 0.0 0.0 0.0 10.852566735112937 6.704940703180656E-4 60-61 0.0 0.0 0.0 11.895017390939948 6.704940703180656E-4 62-63 0.0 0.0 0.0 12.90139546578385 7.710681808657755E-4 64-65 0.0 0.0 0.0 13.89297238402548 7.710681808657755E-4 66-67 0.0 0.0 0.0 14.965327075388677 7.710681808657755E-4 68-69 0.0 0.0 0.0 16.10023886351255 7.710681808657755E-4 70-71 0.0 0.0 0.0 17.231077400159243 7.710681808657755E-4 72-73 0.0 0.0 0.0 18.311159535682854 8.213552361396304E-4 74-75 0.0 0.0 0.0 19.34075346771152 8.548799396555337E-4 76-77 0.0 0.0 0.0 20.405296903155513 8.884046431714369E-4 78-79 0.0 0.0 0.0 21.519339563340736 9.386916984452919E-4 80-81 0.0 0.0 0.0 22.63909818547542 9.386916984452919E-4 82-83 0.0 0.0 0.0 23.720471022084396 9.722164019611952E-4 84-85 0.0 0.0 0.0 24.733771948204332 9.722164019611952E-4 86-87 0.0 0.0 0.0 25.76623224238361 9.722164019611952E-4 88 0.0 0.0 0.0 26.55122993756024 9.722164019611952E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5680 0.0 57.40373 1 TACCTGG 2560 0.0 42.098545 2 GTATCAA 10565 0.0 41.565094 1 ATCAACG 12620 0.0 34.379253 3 CAACGCA 12665 0.0 34.1823 5 TATCAAC 12885 0.0 33.89496 2 TCAACGC 13020 0.0 33.21475 4 GTGGTAT 2050 0.0 32.637493 1 ACCTGGG 3225 0.0 32.21192 3 AACGCAG 13525 0.0 32.182556 6 ACGCAGA 14995 0.0 28.58875 7 CGCAGAG 15100 0.0 28.075367 8 ATAACGC 290 0.0 27.555294 3 TAACGCA 295 0.0 27.088253 4 TGGTATC 2465 0.0 26.347155 2 GTACATG 8185 0.0 26.134819 1 GCAGAGT 16135 0.0 25.604898 9 TACATGG 8280 0.0 24.95202 2 GTACTAG 955 0.0 24.668854 1 CCTGGGG 3125 0.0 23.31502 4 >>END_MODULE