FastQCFastQC Report
Mon 6 Jun 2016
ERR1211440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211440_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2982875
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139460.46753551523278714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120490.40393915266311864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74340.249222645937225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41250.13828940200310105No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35690.11964966684825881No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34210.11468801072790512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53700.060.1460761
TACCTGG24450.042.6708682
GTATCAA99350.041.3588451
ATCAACG121400.033.2929043
CAACGCA122700.033.011795
TATCAAC123900.032.9993442
ACCTGGG30900.032.700313
TCAACGC126600.032.0367934
AACGCAG130950.031.5056276
GTGGTAT19150.028.9693051
ACGCAGA148350.027.4302317
CGCAGAG149800.027.0392448
GTACATG84050.026.2337271
TACATGG85050.024.8101482
TCTAACG4400.024.565892
GCAGAGT161500.024.323889
TGGTATC21950.024.1936452
ACATGGG85750.023.9507543
GTACCTG44850.023.1661931
ATAACGC2853.6379788E-1223.086343
Produced by FastQC (version 0.11.5)