##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211440_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2982875 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.041513640363746 33.0 31.0 34.0 30.0 34.0 2 32.16405280140804 33.0 31.0 34.0 30.0 34.0 3 32.24094472614508 34.0 31.0 34.0 30.0 34.0 4 35.891582114570674 37.0 35.0 37.0 35.0 37.0 5 35.75645275112098 37.0 35.0 37.0 35.0 37.0 6 35.80016929975275 37.0 35.0 37.0 35.0 37.0 7 35.73097866990739 37.0 35.0 37.0 33.0 37.0 8 35.71963122826133 37.0 35.0 37.0 33.0 37.0 9 37.30150140384696 39.0 37.0 39.0 34.0 39.0 10-11 37.283464945731886 39.0 37.0 39.0 34.0 39.0 12-13 37.202945983321456 39.0 37.0 39.0 33.0 39.0 14-15 38.40065959854168 40.0 38.0 41.0 33.5 41.0 16-17 38.39811440305075 40.0 38.0 41.0 33.5 41.0 18-19 38.41443272011063 40.0 38.0 41.0 33.5 41.0 20-21 38.37663529313163 40.0 38.0 41.0 34.0 41.0 22-23 38.26658877760549 40.0 38.0 41.0 34.0 41.0 24-25 38.143473829778316 40.0 37.0 41.0 33.0 41.0 26-27 38.011681682940115 40.0 37.0 41.0 33.0 41.0 28-29 37.925944432803924 40.0 37.0 41.0 33.0 41.0 30-31 37.936251770523405 40.0 37.0 41.0 33.0 41.0 32-33 37.742785902862174 40.0 37.0 41.0 33.0 41.0 34-35 37.60606998281859 40.0 36.0 41.0 33.0 41.0 36-37 37.4273229686125 40.0 36.0 41.0 32.0 41.0 38-39 37.297773959686545 40.0 36.0 41.0 32.0 41.0 40-41 37.21043322298118 39.5 36.0 41.0 31.0 41.0 42-43 37.04697682604869 39.0 36.0 41.0 31.0 41.0 44-45 36.869007584964166 39.0 35.5 41.0 31.0 41.0 46-47 36.61337048987973 39.0 35.0 41.0 30.0 41.0 48-49 36.36524980094707 39.0 35.0 41.0 29.5 41.0 50-51 36.16556443028957 39.0 35.0 40.5 29.0 41.0 52-53 35.94930394334325 39.0 35.0 40.0 28.0 41.0 54-55 35.74304689267905 38.0 35.0 40.0 28.0 41.0 56-57 35.51181377027197 38.0 34.5 40.0 27.0 41.0 58-59 35.244338264258474 38.0 34.0 40.0 26.5 41.0 60-61 34.987298160331896 37.5 34.0 40.0 26.0 41.0 62-63 34.701434354439925 37.0 34.0 40.0 26.0 41.0 64-65 34.359151154506975 36.5 33.0 39.5 26.0 41.0 66-67 34.0062759921217 36.0 33.0 39.0 25.0 40.5 68-69 33.63588333403177 35.5 33.0 39.0 24.5 40.0 70-71 33.2608734861501 35.0 32.0 38.0 24.0 40.0 72-73 32.900674517034744 35.0 32.0 37.5 23.0 40.0 74-75 32.5212338767129 35.0 31.5 37.0 22.5 39.0 76-77 31.481624271885345 34.0 30.5 36.0 21.5 38.5 78-79 31.93020961320873 34.5 31.0 36.0 22.0 38.5 80-81 31.793504421070274 35.0 31.0 35.5 22.0 37.5 82-83 31.500285462850435 34.0 31.0 35.0 20.5 37.0 84-85 31.20678104178016 34.0 31.0 35.0 20.0 36.5 86-87 30.91738088253782 34.0 30.5 35.0 19.5 36.0 88-89 30.646139211331352 34.0 30.0 35.0 19.0 36.0 90-91 30.403685370657502 34.0 30.0 35.0 18.0 35.0 92-93 30.19885060553996 34.0 29.0 35.0 18.0 35.0 94-95 30.002040648703016 34.0 29.0 35.0 18.0 35.0 96-97 29.794752210535137 34.0 29.0 35.0 17.0 35.0 98-99 29.560686250680973 34.0 29.0 35.0 15.5 35.0 100 27.776627247202782 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2751441433734634 1101 2 -0.2331213641236083 1101 3 -0.2586373304275895 1101 4 -0.16573601851310116 1101 5 -0.21951698028897937 1101 6 -0.2511280646487535 1101 7 -0.2080566967238724 1101 8 -0.2933065094499412 1101 9 -0.4942448177894292 1101 10-11 -0.31201854670720053 1101 12-13 -0.4263516262160465 1101 14-15 -0.6507888413953751 1101 16-17 -0.561077684967465 1101 18-19 -0.5818393182193944 1101 20-21 -0.7137659787175537 1101 22-23 -0.7210454248941076 1101 24-25 -0.7698656940658211 1101 26-27 -0.8518718176506042 1101 28-29 -0.8055003703709289 1101 30-31 -0.6932154642896151 1101 32-33 -0.6832340055748389 1101 34-35 -0.7167679309213284 1101 36-37 -0.7107191332945177 1101 38-39 -0.6808214407911279 1101 40-41 -0.6206700007350534 1101 42-43 -0.6564444334428501 1101 44-45 -0.6618001582449651 1101 46-47 -0.6771831051032322 1101 48-49 -0.5996484953662033 1101 50-51 -0.720613460080294 1101 52-53 -0.6163668841970988 1101 54-55 -0.5481545637661043 1101 56-57 -0.7599768677946557 1101 58-59 -0.6460418615952506 1101 60-61 -0.5644677368913307 1101 62-63 -0.5897563609908829 1101 64-65 -0.5893318855507346 1101 66-67 -0.5477995791970898 1101 68-69 -0.7243574106632806 1101 70-71 -0.6718019326841471 1101 72-73 -0.6761999319519134 1101 74-75 -0.6832575195175004 1101 76-77 -0.612817362012283 1101 78-79 -0.6608570488410734 1101 80-81 -0.6004130617435024 1101 82-83 -0.6591550045324617 1101 84-85 -0.7382078445627158 1101 86-87 -0.9353481675953077 1101 88-89 -1.0384506519287413 1101 90-91 -1.1011382736151987 1101 92-93 -1.3838166595476054 1101 94-95 -1.6202271869506575 1101 96-97 -1.5068882864579436 1101 98-99 -1.7728419137467206 1101 100 -1.4381655224424073 1102 1 -0.3631592795879719 1102 2 -0.33744309481642887 1102 3 -0.3805439352790927 1102 4 -0.21911689152208425 1102 5 -0.25997257948122865 1102 6 -0.3618421199211923 1102 7 -0.30625037222637985 1102 8 -0.3748305287897651 1102 9 -0.44221215088216326 1102 10-11 -0.56727622634169 1102 12-13 -0.534948361933381 1102 14-15 -0.6527372878711049 1102 16-17 -0.7697140456569045 1102 18-19 -0.6581765105238446 1102 20-21 -0.6748950696833376 1102 22-23 -0.873626728637511 1102 24-25 -0.8703555186137919 1102 26-27 -0.95756460016338 1102 28-29 -0.8653694273583383 1102 30-31 -0.8743589326215968 1102 32-33 -0.705912548721912 1102 34-35 -0.7558311762050067 1102 36-37 -0.6364452335088089 1102 38-39 -0.6731804742196701 1102 40-41 -0.6840556003379064 1102 42-43 -0.749229230790526 1102 44-45 -0.8663938301662526 1102 46-47 -0.8684906797863405 1102 48-49 -0.8266563638637834 1102 50-51 -0.8432357688006604 1102 52-53 -0.7972036333254948 1102 54-55 -0.9111018651773861 1102 56-57 -0.9144496580811534 1102 58-59 -0.9008703350165774 1102 60-61 -0.9772491135557928 1102 62-63 -0.851742104645254 1102 64-65 -0.8526892984769106 1102 66-67 -0.989145976164906 1102 68-69 -1.0576795269547787 1102 70-71 -0.9840671659157749 1102 72-73 -1.1597212999535174 1102 74-75 -1.0741513860287313 1102 76-77 -0.9853661939631984 1102 78-79 -0.9311134055849912 1102 80-81 -0.8394776036760483 1102 82-83 -0.8486842407229176 1102 84-85 -0.8752469592108625 1102 86-87 -0.9309676554869171 1102 88-89 -1.00895621223696 1102 90-91 -1.0530085969963388 1102 92-93 -1.3051827779273282 1102 94-95 -1.260989381856124 1102 96-97 -1.3326772587062585 1102 98-99 -1.575156447420845 1102 100 -1.7027547517976735 1103 1 -0.31756008391198165 1103 2 -0.2681240838252421 1103 3 -0.27159609664866835 1103 4 -0.20083134645041412 1103 5 -0.12716026188082452 1103 6 -0.2692730441342803 1103 7 -0.26146640574697244 1103 8 -0.3528960314287346 1103 9 -0.35930199691215847 1103 10-11 -0.3339093696568227 1103 12-13 -0.4043582347032455 1103 14-15 -0.6460037784194981 1103 16-17 -0.6568128552183552 1103 18-19 -0.6968743337126426 1103 20-21 -0.715627419461434 1103 22-23 -0.7899413902619798 1103 24-25 -0.6307446298588104 1103 26-27 -0.7030666539735293 1103 28-29 -0.6932885374714601 1103 30-31 -0.5973239701203639 1103 32-33 -0.5946229025316754 1103 34-35 -0.5237142785148663 1103 36-37 -0.5540429538815062 1103 38-39 -0.5734894840612341 1103 40-41 -0.6382001771058441 1103 42-43 -0.6667687310610901 1103 44-45 -0.7447221655353147 1103 46-47 -0.7520478157237775 1103 48-49 -0.6005319992238967 1103 50-51 -0.6455370734924557 1103 52-53 -0.6461871162529675 1103 54-55 -0.6121140083539132 1103 56-57 -0.6764630953989368 1103 58-59 -0.6908397650299491 1103 60-61 -0.6061373371658973 1103 62-63 -0.6661210513106184 1103 64-65 -0.7641096295867413 1103 66-67 -0.7229905627741147 1103 68-69 -0.5865971857028214 1103 70-71 -0.6644932572693207 1103 72-73 -0.6742799457716728 1103 74-75 -0.7762589980558836 1103 76-77 -0.6230412199923094 1103 78-79 -0.5805980985744341 1103 80-81 -0.4525224234020797 1103 82-83 -0.4160830965944733 1103 84-85 -0.6780928582843977 1103 86-87 -0.6174219655624569 1103 88-89 -0.6698873614988194 1103 90-91 -0.9212564319917043 1103 92-93 -1.0876045036598114 1103 94-95 -0.9611285190342116 1103 96-97 -1.1767333113656058 1103 98-99 -1.4062743383991219 1103 100 -1.358325514194341 1104 1 -0.3147557266955303 1104 2 -0.3269354688824535 1104 3 -0.35723333927444756 1104 4 -0.23868134485368842 1104 5 -0.22479284766568952 1104 6 -0.3699133597596287 1104 7 -0.2824751339621514 1104 8 -0.3557068315795533 1104 9 -0.6469669710479806 1104 10-11 -0.4941424161955439 1104 12-13 -0.5859970202717477 1104 14-15 -0.7071153610786993 1104 16-17 -0.6565622810968819 1104 18-19 -0.7517524474007473 1104 20-21 -0.6556252120561012 1104 22-23 -0.7836696838760204 1104 24-25 -0.7652135723940034 1104 26-27 -0.9418067157472692 1104 28-29 -0.9904173418141085 1104 30-31 -0.8059459048943367 1104 32-33 -0.6903198767616203 1104 34-35 -0.7793379797733664 1104 36-37 -0.7533957471176507 1104 38-39 -0.8664935498329456 1104 40-41 -0.9325489252997201 1104 42-43 -1.0094488012722636 1104 44-45 -1.0079700991742087 1104 46-47 -0.9257552790508825 1104 48-49 -1.0079892615594517 1104 50-51 -1.0201871241115157 1104 52-53 -0.9623347625369902 1104 54-55 -0.8466425620384044 1104 56-57 -1.0053904420697037 1104 58-59 -1.022861961218986 1104 60-61 -1.1430956894778816 1104 62-63 -1.1886447094854944 1104 64-65 -0.9971482546981676 1104 66-67 -1.0857304290187955 1104 68-69 -1.1591058990712355 1104 70-71 -1.2303193170900073 1104 72-73 -1.0388627013418734 1104 74-75 -1.126886007621117 1104 76-77 -0.9930299476584388 1104 78-79 -0.8824812282270003 1104 80-81 -1.1115816653432304 1104 82-83 -1.0305259984697024 1104 84-85 -1.0229021082091805 1104 86-87 -0.9065591502018293 1104 88-89 -0.9799234643414962 1104 90-91 -0.9504371120406248 1104 92-93 -1.2956077066385774 1104 94-95 -1.1356145969735465 1104 96-97 -1.269350983158045 1104 98-99 -1.2358187360996133 1104 100 -1.5291096361148284 1105 1 -0.44056247109491764 1105 2 -0.34837517740096047 1105 3 -0.4013915535613215 1105 4 -0.31935092244540186 1105 5 -0.30685143570496365 1105 6 -0.37310792171079044 1105 7 -0.41330954511572315 1105 8 -0.37304109848349754 1105 9 -0.505505090227345 1105 10-11 -0.5230835104829339 1105 12-13 -0.581667910697135 1105 14-15 -0.7407243084454151 1105 16-17 -0.7794751186653244 1105 18-19 -0.7675996465268184 1105 20-21 -0.7916550409338967 1105 22-23 -0.8021267477315988 1105 24-25 -0.7854020369620756 1105 26-27 -0.8747423901385858 1105 28-29 -0.7336335574170647 1105 30-31 -0.7731412707535341 1105 32-33 -0.7306964007021932 1105 34-35 -0.8045994930005733 1105 36-37 -0.8468913855924285 1105 38-39 -0.8321118944955472 1105 40-41 -0.8801137809042245 1105 42-43 -0.9213057964066778 1105 44-45 -0.9329529999207438 1105 46-47 -0.9578026026530395 1105 48-49 -0.7870827093848476 1105 50-51 -0.8427981720121167 1105 52-53 -0.89640701052965 1105 54-55 -0.9394785421092635 1105 56-57 -0.9322348113098471 1105 58-59 -0.9124299578996187 1105 60-61 -1.1359723511849538 1105 62-63 -0.9955390312367243 1105 64-65 -1.0299241199528026 1105 66-67 -0.9629229423011054 1105 68-69 -1.0652395066409355 1105 70-71 -0.9858356497134366 1105 72-73 -1.163211957240712 1105 74-75 -0.9860773020751239 1105 76-77 -0.9899707210309288 1105 78-79 -1.0551366855631485 1105 80-81 -0.9754562103085966 1105 82-83 -0.9798489976949689 1105 84-85 -0.7635060279091448 1105 86-87 -0.8834143786835114 1105 88-89 -0.9040230387240626 1105 90-91 -0.8441271633545959 1105 92-93 -0.836058786565836 1105 94-95 -0.9517291441431759 1105 96-97 -1.1468915594434073 1105 98-99 -1.170111815683267 1105 100 -1.15029471973099 1106 1 -0.4075445294932578 1106 2 -0.3734113185014962 1106 3 -0.4587784159459467 1106 4 -0.2557414024891287 1106 5 -0.21302417005427543 1106 6 -0.28522767119558523 1106 7 -0.31709495570465407 1106 8 -0.42057581405126854 1106 9 -0.5460978752570043 1106 10-11 -0.5529942638503869 1106 12-13 -0.5759332467088143 1106 14-15 -0.7895147941676157 1106 16-17 -0.6973040973213713 1106 18-19 -0.7479444480051143 1106 20-21 -0.7271280930409674 1106 22-23 -0.7138844947036773 1106 24-25 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0.9042033270890784 2311 34-35 0.859790002659004 2311 36-37 0.8661212685935311 2311 38-39 0.9121188602299739 2311 40-41 0.9084840424316383 2311 42-43 1.0866845732038328 2311 44-45 1.0586859891188354 2311 46-47 0.9842886149278982 2311 48-49 1.0122214947649937 2311 50-51 1.0422265346176118 2311 52-53 1.1205712546760722 2311 54-55 1.0889884525823277 2311 56-57 1.0295108738297998 2311 58-59 1.1239443316171531 2311 60-61 1.1165070068784786 2311 62-63 1.127668313370478 2311 64-65 1.1767234133232023 2311 66-67 1.1531121045727701 2311 68-69 1.1572125593126898 2311 70-71 1.068455974839658 2311 72-73 1.1315647234889568 2311 74-75 1.050181541775281 2311 76-77 0.8855196611507985 2311 78-79 0.9638881735973257 2311 80-81 1.0291585783022192 2311 82-83 1.057259241312341 2311 84-85 1.1219643283255891 2311 86-87 1.0858430270197985 2311 88-89 1.1247027134955978 2311 90-91 1.3141819494360298 2311 92-93 1.4483879165070732 2311 94-95 1.4783318388253406 2311 96-97 1.4823747969656509 2311 98-99 1.50255299714809 2311 100 1.5593619436001127 2312 1 0.12658202942967733 2312 2 0.22320646837692237 2312 3 0.3232236119873164 2312 4 0.23666761562330407 2312 5 0.30215100885086343 2312 6 0.3969256345763128 2312 7 0.40506768842554663 2312 8 0.20778714143018107 2312 9 0.4225892068358661 2312 10-11 0.22073849329395756 2312 12-13 0.3558596933219462 2312 14-15 0.711019378152038 2312 16-17 0.8239419554443188 2312 18-19 0.8041427018175895 2312 20-21 0.7808997453301529 2312 22-23 0.5045357736146556 2312 24-25 0.3937686765940356 2312 26-27 0.7050614537892912 2312 28-29 0.8958435467257502 2312 30-31 0.9184822851928942 2312 32-33 0.7322804003956662 2312 34-35 0.8448409397447634 2312 36-37 0.9715128272118392 2312 38-39 0.8963626316741369 2312 40-41 0.9571316985647158 2312 42-43 0.8442762324869406 2312 44-45 0.7817402739092927 2312 46-47 0.897314879032173 2312 48-49 0.9766055180479896 2312 50-51 0.9710604174814179 2312 52-53 0.9817919701540134 2312 54-55 1.0443931626721152 2312 56-57 0.9781334979604281 2312 58-59 1.0636991064064958 2312 60-61 1.0650195880844748 2312 62-63 1.074317624725957 2312 64-65 1.1171378536073107 2312 66-67 1.1996761287146427 2312 68-69 1.1199912899888673 2312 70-71 0.9953837232130027 2312 72-73 1.0179697569186388 2312 74-75 1.1533076686495676 2312 76-77 1.059454778768842 2312 78-79 0.9681823737510413 2312 80-81 0.882190845034895 2312 82-83 0.9318620526643464 2312 84-85 0.7629986244582092 2312 86-87 0.9873132841697441 2312 88-89 0.601789589937443 2312 90-91 0.9486369575100575 2312 92-93 1.1213061238273099 2312 94-95 1.1040821279051052 2312 96-97 1.2566917966871465 2312 98-99 1.3487060417532106 2312 100 1.4165272536496083 2313 1 -0.41138193939504575 2313 2 -0.05549623070947263 2313 3 0.2507558940934089 2313 4 0.08464448616507525 2313 5 0.22506147613135852 2313 6 0.35808324541336845 2313 7 0.16694771776371908 2313 8 0.16306853124653742 2313 9 0.27632215913833846 2313 10-11 0.3890882793840973 2313 12-13 0.537641183080126 2313 14-15 0.6355221572964282 2313 16-17 0.733519615545184 2313 18-19 0.574550975940582 2313 20-21 0.8663047217835356 2313 22-23 0.8267069327106142 2313 24-25 0.7147734992631882 2313 26-27 0.8909768466301031 2313 28-29 0.8498733303608361 2313 30-31 0.8265972927368779 2313 32-33 0.992044552617557 2313 34-35 0.9207035064046991 2313 36-37 0.789979456012361 2313 38-39 0.5209785537773044 2313 40-41 0.7541143065961506 2313 42-43 0.6344874737435262 2313 44-45 0.9638355145503184 2313 46-47 1.002019865468533 2313 48-49 0.952359750306222 2313 50-51 0.9214774366076028 2313 52-53 0.9677073405947212 2313 54-55 1.0442625326936437 2313 56-57 1.0158279187872736 2313 58-59 0.9498470347957308 2313 60-61 0.9551061279251982 2313 62-63 1.0892411010947072 2313 64-65 1.0161100693314253 2313 66-67 1.0311090242178977 2313 68-69 1.0614957157653961 2313 70-71 0.9995022448863509 2313 72-73 1.060696661265915 2313 74-75 0.6651436631035992 2313 76-77 0.8356569535059855 2313 78-79 0.7623468611980542 2313 80-81 0.9426598316710759 2313 82-83 0.9918975971598947 2313 84-85 1.064555372413139 2313 86-87 1.1629710316848083 2313 88-89 1.1896121356637224 2313 90-91 1.3337003472097848 2313 92-93 1.146852516659095 2313 94-95 1.1093645966601215 2313 96-97 1.3401327314407787 2313 98-99 1.4983852236354593 2313 100 1.4926995765059843 2314 1 -0.39371132894688543 2314 2 -0.21967083526076792 2314 3 0.10688971007969172 2314 4 -0.28037749268737144 2314 5 0.1457566812874802 2314 6 0.17178871393677042 2314 7 0.07429439382955394 2314 8 0.1740095921860032 2314 9 0.26528581677413854 2314 10-11 0.39637238127625096 2314 12-13 0.5445897349510176 2314 14-15 0.6168768867076935 2314 16-17 0.7923908559955848 2314 18-19 0.8584923025811833 2314 20-21 0.6653088461287595 2314 22-23 0.776936336326429 2314 24-25 0.9735829915537622 2314 26-27 0.9322238131122802 2314 28-29 0.8950298060088997 2314 30-31 0.7847251279479792 2314 32-33 0.857240356669962 2314 34-35 0.9347758844935683 2314 36-37 0.8362732223392726 2314 38-39 0.8461468486694699 2314 40-41 0.9152857348342778 2314 42-43 0.6505512698380826 2314 44-45 0.7513474310684316 2314 46-47 0.934902651708974 2314 48-49 0.8252974056049851 2314 50-51 0.898108562215981 2314 52-53 0.9503058729999339 2314 54-55 1.0435420167334968 2314 56-57 0.9683049496238922 2314 58-59 0.941688046543895 2314 60-61 0.8702795752600352 2314 62-63 0.7015129066110433 2314 64-65 1.0034664054121265 2314 66-67 1.115138835783121 2314 68-69 1.1267898661887514 2314 70-71 1.085703386075231 2314 72-73 1.01337066742812 2314 74-75 1.0001413781608406 2314 76-77 0.8630021065181701 2314 78-79 0.9110166781656943 2314 80-81 0.8526563457476151 2314 82-83 0.9135863013694667 2314 84-85 0.7888736772935516 2314 86-87 0.9901480256623714 2314 88-89 1.0518998999129892 2314 90-91 1.2008144597944614 2314 92-93 1.3467971447467093 2314 94-95 1.3909500085595496 2314 96-97 1.4636191244200667 2314 98-99 1.5264550589058317 2314 100 1.6235847351452115 2315 1 -0.11408619548790355 2315 2 0.0986587827512011 2315 3 0.17534790139720968 2315 4 -0.0573781996841376 2315 5 0.2203421533688399 2315 6 0.3637257120969579 2315 7 0.4266439895606311 2315 8 0.35002204946164994 2315 9 0.6055479392292114 2315 10-11 0.575726419567431 2315 12-13 0.6292273319422463 2315 14-15 0.759643860794661 2315 16-17 0.8001533198038615 2315 18-19 0.8243611780514826 2315 20-21 0.8160240684941513 2315 22-23 0.8834677940021223 2315 24-25 0.8206761528229976 2315 26-27 0.9677710657802621 2315 28-29 0.951781404296355 2315 30-31 0.9133997905603621 2315 32-33 0.9501086694135381 2315 34-35 0.9612091354972918 2315 36-37 0.9410541545518569 2315 38-39 1.0533498244597084 2315 40-41 1.0184574305260838 2315 42-43 1.0946017319622356 2315 44-45 0.8573687543264157 2315 46-47 0.8636157372585203 2315 48-49 1.0130890693733718 2315 50-51 0.9340630832965147 2315 52-53 0.9141172443491641 2315 54-55 0.8451745131166248 2315 56-57 0.9208406978609034 2315 58-59 0.975904739396313 2315 60-61 1.0167625699820348 2315 62-63 1.0836166507496117 2315 64-65 1.0702685460555372 2315 66-67 1.063007043636489 2315 68-69 1.0211055165061609 2315 70-71 0.6835132418530421 2315 72-73 0.8798363129426647 2315 74-75 1.0226116933135714 2315 76-77 1.0469953832402403 2315 78-79 1.0130218844000751 2315 80-81 0.9705320284080123 2315 82-83 0.891338022212846 2315 84-85 0.8826890197490158 2315 86-87 0.9671584856077473 2315 88-89 1.0371381313243262 2315 90-91 1.0132719562603967 2315 92-93 1.1553844871859873 2315 94-95 1.2470614085787588 2315 96-97 1.375567921807896 2315 98-99 1.2832372409679884 2315 100 1.2169808480247326 2316 1 0.3642888842500014 2316 2 0.43619588622795646 2316 3 0.4085221891026407 2316 4 0.280288696824357 2316 5 0.363291110046724 2316 6 0.3773130513766816 2316 7 0.3848841877899929 2316 8 0.44379603997052897 2316 9 0.5584020953717044 2316 10-11 0.44833861495018823 2316 12-13 0.5536800441372591 2316 14-15 0.673892333329789 2316 16-17 0.7315335507027143 2316 18-19 0.7556001852822831 2316 20-21 0.8018710970818006 2316 22-23 0.8120163613009268 2316 24-25 0.9035211644564356 2316 26-27 0.8172271887296603 2316 28-29 0.7920539327899832 2316 30-31 0.643438027882496 2316 32-33 0.6321861691277633 2316 34-35 0.5497519506049002 2316 36-37 0.56338095275688 2316 38-39 0.5247890890641926 2316 40-41 0.5849955332616972 2316 42-43 0.7395327978506359 2316 44-45 0.7196612974954988 2316 46-47 0.6955457448912412 2316 48-49 0.3887729717188648 2316 50-51 0.4729320402693915 2316 52-53 0.5994825269255486 2316 54-55 0.7073376319841813 2316 56-57 0.7140037024220973 2316 58-59 0.6012608405906263 2316 60-61 0.6293582093786441 2316 62-63 0.6804848407636612 2316 64-65 0.7832926499489545 2316 66-67 0.790339723088266 2316 68-69 0.7666779885840711 2316 70-71 0.5547428833366652 2316 72-73 0.617316078872733 2316 74-75 0.5722151239793902 2316 76-77 0.5553721960101008 2316 78-79 0.45582279590114183 2316 80-81 0.4787534213969771 2316 82-83 0.3969346092460775 2316 84-85 0.5510868132674815 2316 86-87 0.46739484166837997 2316 88-89 0.4876329672578237 2316 90-91 0.6293412185418781 2316 92-93 0.6916245821708884 2316 94-95 0.648821542206587 2316 96-97 0.8143917093308026 2316 98-99 0.8926570071192934 2316 100 0.6308060182859059 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 25.0 13 116.0 14 506.0 15 1474.0 16 3645.0 17 7078.0 18 11313.0 19 15211.0 20 20254.0 21 25868.0 22 29781.0 23 30140.0 24 29599.0 25 30992.0 26 34903.0 27 41816.0 28 50381.0 29 61431.0 30 74858.0 31 91865.0 32 114877.0 33 147073.0 34 200453.0 35 279515.0 36 413410.0 37 623998.0 38 557700.0 39 84577.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66591755274545 19.64797236105055 12.834501727434342 23.851608358769663 2 16.019276704521644 21.598256715417172 39.001869002221014 23.38059757784017 3 18.75429535407798 26.453346671699844 30.88544806116517 23.906909913057003 4 12.49286624895802 17.499636187079645 38.539859477678654 31.467638086283685 5 12.541149231528987 37.59260358173908 35.10036573742072 14.765881449311221 6 29.2515442316557 37.779055441478434 18.507077903029796 14.462322423836063 7 25.747937890647528 33.4109320291746 22.954988286441104 17.886141793736765 8 25.402759297198223 34.93652570468419 21.06118924079935 18.599525757318226 9 26.129782790169067 15.863324426802736 21.08460757066276 36.92228521236544 10-11 24.377152824584638 26.225013657219954 28.93863914582491 20.459194372370494 12-13 25.000645352273498 24.604482868327686 28.671241459977935 21.723630319420877 14-15 22.341138110426385 25.945212570158848 26.876361522477985 24.83728779693678 16-17 21.350195382168593 28.467999967816255 27.77070219573619 22.411102454278964 18-19 20.947843942505134 27.524167120647025 30.201684616351677 21.326304320496163 20-21 21.851539513311742 26.98598281623984 30.07266983575742 21.089807834690998 22-23 21.777366094349595 26.935595178409283 29.754134811907107 21.532903915334018 24-25 21.369565516198644 26.983498794618477 30.144142911883947 21.502792777298932 26-27 21.39630390143737 27.062129656790844 29.872052969031554 21.669513472740228 28-29 21.134785559627545 27.519276476103105 29.912963980199443 21.432973984069907 30-31 21.728274868108144 27.183207689714656 29.849189103745537 21.23932833843166 32-33 21.6004256875763 27.36984022298304 29.51923389647973 21.510500192960933 34-35 21.767701510534714 27.21628307260204 29.812480027255145 21.203535389608103 36-37 21.63681944160093 26.955864739529073 29.763152154028816 21.644163664841173 38-39 21.425729626416185 27.004966866598767 29.935711561272694 21.633591945712357 40-41 21.50607946271888 26.557874369671765 30.251631775499472 21.684414392109883 42-43 21.13108413708666 26.82306408162588 29.87312210541102 22.172729675876436 44-45 20.926655593501746 27.103040784071275 29.994977512802652 21.975326109624334 46-47 20.95318230656894 26.86457768047527 29.67008851610831 22.512151496847476 48-49 20.93461478307267 26.881628502617833 29.666490149663954 22.51726656464554 50-51 20.77303764536387 27.090063782487416 29.39266740283552 22.744231169313192 52-53 20.575065903166774 27.237488792605703 29.20973871431321 22.97770658991432 54-55 20.395499272526898 27.0825855897796 29.350886789628255 23.171028348065242 56-57 20.674274982239776 26.882893828561123 28.974660963279668 23.468170225919433 58-59 20.42716684293736 26.9470703441612 29.00854062113334 23.6172221917681 60-61 20.568612637397177 27.097833376888293 28.681887266804267 23.651666718910267 62-63 20.552516786426633 26.81706352818278 28.547192333216497 24.083227352174095 64-65 20.15924315992267 26.791686736504396 28.504243963833886 24.54482613973905 66-67 20.358530399669025 26.816025265096865 28.236717402237694 24.588726932996412 68-69 20.374982871999624 26.994298828721508 28.025903406631393 24.604814892647482 70-71 20.326383468711523 26.81497725398227 27.923801595290264 24.93483768201594 72-73 20.395685293020254 27.028578854917352 27.715578138035408 24.86015771402699 74-75 20.549256492174916 27.219918223025857 27.346326122656073 24.884499162143157 76-77 20.62331322165944 27.077151832854014 27.281627596900886 25.017907348585656 78-79 20.52754012317842 27.195094187667205 27.18361054456609 25.093755144588286 80-81 20.352077875664726 27.363864763581674 27.14420582498493 25.13985153576867 82-83 20.6177269930992 26.98974579697057 27.29812472680246 25.09440248312777 84-85 20.796524364648565 26.977949081995767 26.906993448301247 25.31853310505442 86-87 20.565489497108256 27.09555556971735 27.02309002732593 25.31586490584846 88-89 20.695873716590256 26.738212505734094 27.04846997179706 25.517443805878585 90-91 20.745022188350717 26.562184812654525 27.014788600729577 25.678004398265173 92-93 20.73092214335152 26.460398994532703 27.105264216846138 25.703414645269635 94-95 20.448659544953543 26.14878485245984 27.493556512008986 25.908999090577627 96-97 20.544085590354726 26.065604878156716 27.473040124501892 25.91726940698667 98-99 20.469925640878188 26.287226296279965 27.25310184555682 25.98974621728503 100 20.385331400160144 26.15098467097383 27.460805628913903 26.00287829995212 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2276.0 1 1538.5 2 1732.0 3 2804.0 4 2051.5 5 1278.0 6 1788.0 7 2840.0 8 3968.5 9 4423.0 10 3748.0 11 2952.5 12 2939.0 13 3188.5 14 3502.0 15 3861.0 16 4234.5 17 4634.5 18 5186.5 19 5643.0 20 6250.5 21 7280.5 22 8283.0 23 9542.5 24 11399.5 25 13707.0 26 16492.0 27 19976.5 28 23838.5 29 28300.0 30 33547.5 31 38638.0 32 44847.0 33 50676.5 34 55826.5 35 61712.5 36 67974.0 37 75593.5 38 80709.0 39 83285.5 40 85202.5 41 87474.5 42 91454.5 43 96661.0 44 103045.5 45 110346.0 46 116454.0 47 121169.0 48 125569.5 49 127678.5 50 127009.5 51 124775.5 52 120003.5 53 113232.0 54 106291.5 55 95947.0 56 84110.0 57 72005.0 58 60188.0 59 50655.0 60 40678.5 61 31338.5 62 24330.0 63 18411.0 64 13265.0 65 9244.0 66 6505.5 67 4880.0 68 3639.5 69 2629.5 70 1940.0 71 1478.0 72 1168.0 73 901.5 74 661.5 75 438.5 76 289.5 77 191.0 78 100.5 79 55.0 80 44.5 81 31.5 82 15.0 83 9.5 84 7.5 85 4.0 86 4.0 87 3.0 88 2.0 89 1.5 90 0.5 91 0.0 92 0.5 93 1.5 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.052801408037547674 2 0.0 3 3.352470351590328E-5 4 0.019209655114612582 5 0.00449231027113104 6 0.0 7 2.3467292461132295E-4 8 4.693458492226459E-4 9 0.0015086116582156475 10-11 6.872564220760173E-4 12-13 2.6819762812722625E-4 14-15 3.352470351590328E-5 16-17 1.0057411054770984E-4 18-19 0.0 20-21 1.676235175795164E-5 22-23 1.676235175795164E-5 24-25 6.704940703180656E-5 26-27 0.0 28-29 9.889787537191467E-4 30-31 0.00112307756778276 32-33 0.013611029627456733 34-35 0.02299794661190965 36-37 0.031496458953191135 38-39 0.02053388090349076 40-41 1.5086116582156475E-4 42-43 0.0015588987134895025 44-45 0.00968863931609605 46-47 0.01419771193898504 48-49 0.005347190210786573 50-51 0.010610568662783388 52-53 0.01572308594895864 54-55 0.009738926371369904 56-57 0.002883124502367682 58-59 0.008263839416670159 60-61 0.0017935716381008255 62-63 0.00112307756778276 64-65 0.019461090390981855 66-67 0.04594560616854544 68-69 0.055164899635418846 70-71 0.04941541298244144 72-73 0.04064870301303273 74-75 0.028898294430708626 76-77 0.02879772032016092 78-79 0.01247118970791602 80-81 0.002313204542597326 82-83 0.02780874156644177 84-85 0.048091187193563256 86-87 0.04887901772618698 88-89 0.06042827808741566 90-91 0.03411138582743159 92-93 0.02611574403888866 94-95 0.007123999497129447 96-97 0.0036541926832334573 98-99 0.02207601726522231 100 0.018740309265389932 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2982875.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.50116920827033 #Duplication Level Percentage of deduplicated Percentage of total 1 81.76154121648497 57.64284252032375 2 12.108067397535319 17.072658167575575 3 3.0109066819893613 6.368173243717311 4 1.141728541571762 3.219727883970499 5 0.5751662443037486 2.027494635627196 6 0.3587581788291012 1.517572264228882 7 0.233981965035801 1.1547201476070816 8 0.15705476133461396 0.8858035443052904 9 0.11431906135650073 0.7253664739582765 >10 0.5187771479775607 5.9546867630905735 >50 0.012661623237946522 0.6027565757865498 >100 0.006065600126164702 0.7818878110452618 >500 5.624938099513192E-4 0.2703850314039658 >1k 2.5567900447988747E-4 0.570764775548203 >5k 5.11358008959775E-5 0.26800564309154506 >10k+ 1.02271601791955E-4 0.9371545187200314 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13946 0.46753551523278714 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12049 0.40393915266311864 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7434 0.249222645937225 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4125 0.13828940200310105 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3569 0.11964966684825881 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3421 0.11468801072790512 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 3.352470351590328E-5 5 0.0 0.0 0.0 0.0 6.704940703180656E-5 6 0.0 0.0 0.0 0.0 6.704940703180656E-5 7 0.0 0.0 0.0 0.0 1.0057411054770984E-4 8 0.0 0.0 0.0 0.0 1.0057411054770984E-4 9 0.0 0.0 0.0 0.0 1.0057411054770984E-4 10-11 0.0 0.0 0.0 1.676235175795164E-5 1.0057411054770984E-4 12-13 0.0 0.0 0.0 5.0287055273854925E-5 1.8438586933746805E-4 14-15 0.0 0.0 0.0 6.704940703180656E-5 2.6819762812722625E-4 16-17 0.0 0.0 0.0 1.1733646230566147E-4 3.0172233164312955E-4 18-19 0.0 0.0 0.0 3.687717386749361E-4 3.352470351590328E-4 20-21 0.0 0.0 0.0 9.386916984452918E-4 3.687717386749361E-4 22-23 0.0 0.0 0.0 0.003486569165653941 3.855340904328877E-4 24-25 0.0 0.0 0.0 0.009537778150274484 4.0229644219083935E-4 26-27 0.0 0.0 0.0 0.021321711436114485 4.3582114570674265E-4 28-29 0.0 0.0 0.0 0.05952311109248627 4.3582114570674265E-4 30-31 0.0 0.0 0.0 0.16613166827305872 4.3582114570674265E-4 32-33 0.0 0.0 0.0 0.39641285672379833 4.861082009805976E-4 34-35 0.0 0.0 0.0 0.7440305074801995 5.363952562544525E-4 36-37 0.0 0.0 0.0 1.2708209361773457 5.363952562544525E-4 38-39 0.0 0.0 0.0 1.9914344382516866 6.369693668021624E-4 40-41 0.0 0.0 0.0 2.720345304446214 6.369693668021624E-4 42-43 0.0 0.0 0.0 3.4139714201902525 6.369693668021624E-4 44-45 0.0 0.0 0.0 4.224313791224908 6.704940703180656E-4 46-47 0.0 0.0 0.0 5.159485395801031 7.207811255919205E-4 48-49 0.0 0.0 0.0 6.098696727150819 7.375434773498722E-4 50-51 0.0 0.0 0.0 7.019285085697524 7.375434773498722E-4 52-53 0.0 0.0 0.0 7.868281439886015 7.543058291078239E-4 54-55 0.0 0.0 0.0 8.778292754473453 7.87830532623727E-4 56-57 0.0 0.0 0.0 9.797661651929765 8.213552361396304E-4 58-59 0.0 0.0 0.0 10.878732766207099 8.381175878975821E-4 60-61 0.0 0.0 0.0 11.921233709089385 8.381175878975821E-4 62-63 0.0 0.0 0.0 12.928282278003604 8.716422914134853E-4 64-65 0.0 0.0 0.0 13.917948288144828 8.716422914134853E-4 66-67 0.0 0.0 0.0 14.988190923186522 8.716422914134853E-4 68-69 0.0 0.0 0.0 16.12253279135063 8.716422914134853E-4 70-71 0.0 0.0 0.0 17.254209445585218 8.716422914134853E-4 72-73 0.0 0.0 0.0 18.334358630515858 9.219293466873402E-4 74-75 0.0 0.0 0.0 19.366567489418763 9.554540502032436E-4 76-77 0.0 0.0 0.0 20.433625277626454 9.722164019611952E-4 78-79 0.0 0.0 0.0 21.549528558856807 9.889787537191467E-4 80-81 0.0 0.0 0.0 22.671080752629592 0.0010057411054770985 82-83 0.0 0.0 0.0 23.752956459791307 0.0010057411054770985 84-85 0.0 0.0 0.0 24.767782759921218 0.0010057411054770985 86-87 0.0 0.0 0.0 25.80009219293467 0.00102250345723505 88 0.0 0.0 0.0 26.585291036332396 0.0010392658089930016 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5370 0.0 60.146076 1 TACCTGG 2445 0.0 42.670868 2 GTATCAA 9935 0.0 41.358845 1 ATCAACG 12140 0.0 33.292904 3 CAACGCA 12270 0.0 33.01179 5 TATCAAC 12390 0.0 32.999344 2 ACCTGGG 3090 0.0 32.70031 3 TCAACGC 12660 0.0 32.036793 4 AACGCAG 13095 0.0 31.505627 6 GTGGTAT 1915 0.0 28.969305 1 ACGCAGA 14835 0.0 27.430231 7 CGCAGAG 14980 0.0 27.039244 8 GTACATG 8405 0.0 26.233727 1 TACATGG 8505 0.0 24.810148 2 TCTAACG 440 0.0 24.56589 2 GCAGAGT 16150 0.0 24.32388 9 TGGTATC 2195 0.0 24.193645 2 ACATGGG 8575 0.0 23.950754 3 GTACCTG 4485 0.0 23.166193 1 ATAACGC 285 3.6379788E-12 23.08634 3 >>END_MODULE