FastQCFastQC Report
Mon 6 Jun 2016
ERR1211439_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211439_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8058448
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257250.3192302041286362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200600.2489313078647402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127640.15839278233228035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG81700.047.8104132
ACCTGGG95650.040.2512783
GGTATCA178150.036.3073121
GTATCAA281650.033.3020441
GTACCTG126600.031.9273591
ATAACGC9250.028.4594883
ATCAACG327700.027.9729943
CAACGCA328200.027.8872365
GTACATG252150.027.7818661
TATCAAC338700.027.3310032
TCAACGC339300.027.1548614
TACATGG253750.027.0963382
TAACGCA10100.026.9949194
AACGCAG347200.026.6325956
CCTGGGG94250.026.4344274
ACATGGG254100.025.844763
ACGCAGA376150.024.3206987
CATGGGG189150.024.0571634
CGCAGAG382000.023.910298
ATGGGGG145350.022.5745185
Produced by FastQC (version 0.11.5)