##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211439_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8058448 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.217383545814283 31.0 31.0 34.0 28.0 34.0 2 31.268620707113826 31.0 31.0 34.0 28.0 34.0 3 31.333757567213937 31.0 31.0 34.0 28.0 34.0 4 34.950028839300074 37.0 35.0 37.0 32.0 37.0 5 35.00594196301819 37.0 35.0 37.0 32.0 37.0 6 35.06746137717833 37.0 35.0 37.0 32.0 37.0 7 34.99639831391851 37.0 35.0 37.0 32.0 37.0 8 34.996390868316084 37.0 35.0 37.0 32.0 37.0 9 36.5499532912541 39.0 37.0 39.0 32.0 39.0 10-11 36.47018085864673 39.0 37.0 39.0 32.0 39.0 12-13 36.3802647854773 39.0 37.0 39.0 32.0 39.0 14-15 37.5466790875861 40.0 37.0 41.0 32.0 41.0 16-17 37.541806561263414 40.0 37.0 41.0 32.0 41.0 18-19 37.54354988702539 40.0 37.0 41.0 32.0 41.0 20-21 37.52977974170709 40.0 37.0 41.0 31.5 41.0 22-23 37.40492027745293 40.0 37.0 41.0 31.0 41.0 24-25 37.31325740390706 40.0 37.0 41.0 31.0 41.0 26-27 37.17730765278873 40.0 36.0 41.0 31.0 41.0 28-29 37.0511023338489 40.0 36.0 41.0 30.0 41.0 30-31 36.89965958705696 39.0 36.0 41.0 30.0 41.0 32-33 36.70489906989535 39.0 35.5 41.0 30.0 41.0 34-35 36.583116686984894 39.0 35.0 41.0 30.0 41.0 36-37 36.455306654581626 39.0 35.0 41.0 29.5 41.0 38-39 36.30592559510218 39.0 35.0 40.5 29.0 41.0 40-41 36.15289085441762 39.0 35.0 40.0 28.5 41.0 42-43 35.96288565738713 38.5 35.0 40.0 27.5 41.0 44-45 35.829835285901204 38.0 35.0 40.0 27.0 41.0 46-47 35.614981755792186 38.0 34.5 40.0 26.5 41.0 48-49 35.37702421111361 38.0 34.0 40.0 26.0 41.0 50-51 34.727075548542345 37.5 33.5 39.5 25.0 40.5 52-53 35.03863802310321 38.0 33.5 39.5 26.0 40.5 54-55 35.451317673080474 38.0 34.0 40.0 26.0 41.0 56-57 35.379823013066535 38.0 34.0 40.0 26.0 41.0 58-59 35.18565342855101 38.0 34.0 40.0 26.0 41.0 60-61 34.972619975955666 38.0 34.0 40.0 26.0 41.0 62-63 34.708430208893816 37.0 33.5 40.0 25.5 41.0 64-65 34.513349841061206 37.0 33.0 40.0 25.0 41.0 66-67 34.32817988029457 36.5 33.0 40.0 25.0 41.0 68-69 34.01498111050664 36.0 33.0 39.0 24.5 41.0 70-71 33.66630447947297 35.5 32.5 39.0 24.0 41.0 72-73 33.30810647410023 35.0 32.0 38.5 23.5 40.0 74-75 32.96029080289406 35.0 32.0 37.5 23.0 40.0 76-77 32.60627027685728 35.0 32.0 37.0 22.5 39.0 78-79 32.24297792825616 35.0 31.0 36.5 21.5 39.0 80-81 31.884940871989247 35.0 31.0 36.0 20.5 38.5 82-83 31.550743952185336 35.0 31.0 36.0 20.0 37.0 84-85 31.247340368765798 34.5 31.0 35.0 20.0 37.0 86-87 30.951676240884098 34.0 30.0 35.0 19.0 36.5 88-89 30.677951387165372 34.0 30.0 35.0 18.0 36.0 90-91 30.42011774475681 34.0 29.5 35.0 18.0 36.0 92-93 30.178209563429583 34.0 29.0 35.0 17.0 35.5 94-95 29.943971779677675 34.0 29.0 35.0 15.5 35.0 96-97 29.697564593082937 34.0 29.0 35.0 14.0 35.0 98-99 29.430245253180264 34.0 28.5 35.0 10.5 35.0 100 28.221238382378345 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4333481664411778 1101 2 -0.3164064666110171 1101 3 -0.39183882878237597 1101 4 -0.3523574653785104 1101 5 -0.2851983672666947 1101 6 -0.3323303582788455 1101 7 -0.3532574520740468 1101 8 -0.3630492972018402 1101 9 -0.41210940865782675 1101 10-11 -0.4868156631186622 1101 12-13 -0.45739990572966605 1101 14-15 -0.7147318320720615 1101 16-17 -0.632459872805299 1101 18-19 -0.6760167502693832 1101 20-21 -0.6953655978320015 1101 22-23 -0.6720919158672274 1101 24-25 -0.7472526881057462 1101 26-27 -0.8060178411162937 1101 28-29 -0.8104729885100639 1101 30-31 -0.8586312191472274 1101 32-33 -0.8718567838838212 1101 34-35 -0.7827995130207128 1101 36-37 -0.8512328923105699 1101 38-39 -0.8496238459668035 1101 40-41 -1.0195543466568537 1101 42-43 -0.9802255228290662 1101 44-45 -1.0410785586533606 1101 46-47 -1.034788012050356 1101 48-49 -1.0344832697787112 1101 50-51 -0.9291878067856274 1101 52-53 -0.7944203079340326 1101 54-55 -0.7550674817263712 1101 56-57 -0.7899807596193398 1101 58-59 -0.7023385302635106 1101 60-61 -0.7945963164201189 1101 62-63 -0.805269431265593 1101 64-65 -0.7271510403639354 1101 66-67 -0.8530498437199014 1101 68-69 -0.8442224569715222 1101 70-71 -0.8279550473087127 1101 72-73 -0.9308278738922766 1101 74-75 -0.9890503121197654 1101 76-77 -0.9310167052255807 1101 78-79 -1.001240736742556 1101 80-81 -1.0339845580149891 1101 82-83 -1.0319518771462342 1101 84-85 -1.0751662223021619 1101 86-87 -1.1524063254682702 1101 88-89 -1.2547685568201352 1101 90-91 -1.374985531624347 1101 92-93 -1.4987686148151802 1101 94-95 -1.5425235738006542 1101 96-97 -1.7496023477475937 1101 98-99 -1.749697035571888 1101 100 -1.7864624848105635 1102 1 -0.37406856547621103 1102 2 -0.34666953291844393 1102 3 -0.4379018045066623 1102 4 -0.370602524427035 1102 5 -0.33131749384235576 1102 6 -0.3022857282711229 1102 7 -0.30590641377062866 1102 8 -0.37117534318395684 1102 9 -0.5382177949815485 1102 10-11 -0.48317478498834987 1102 12-13 -0.5107573169390847 1102 14-15 -0.6863084824262273 1102 16-17 -0.6525995702582961 1102 18-19 -0.6778128443609646 1102 20-21 -0.7679540503015971 1102 22-23 -0.7695046726678783 1102 24-25 -0.7596570020091846 1102 26-27 -0.696691141576224 1102 28-29 -0.8427849355567858 1102 30-31 -0.7410764144931079 1102 32-33 -0.75637746908982 1102 34-35 -0.790011770742943 1102 36-37 -0.8974216683677767 1102 38-39 -0.8758824120983135 1102 40-41 -1.0872433785842688 1102 42-43 -1.0059244995811483 1102 44-45 -1.054098979363829 1102 46-47 -1.2363266575072487 1102 48-49 -1.2162454301253547 1102 50-51 -1.0658170168380323 1102 52-53 -0.7859499390660645 1102 54-55 -0.8587956783119637 1102 56-57 -0.8959879605563188 1102 58-59 -0.8678745177409226 1102 60-61 -0.890507727972313 1102 62-63 -0.7663523414046551 1102 64-65 -0.8722152770241607 1102 66-67 -0.8797781233673589 1102 68-69 -0.8713494852916526 1102 70-71 -0.9933863664940432 1102 72-73 -0.9165413420491859 1102 74-75 -0.9338222193399304 1102 76-77 -0.9457852632328994 1102 78-79 -1.08414345802327 1102 80-81 -1.1053583494024117 1102 82-83 -1.0537291136945903 1102 84-85 -1.215125001022006 1102 86-87 -1.220127767046069 1102 88-89 -1.3215499244668472 1102 90-91 -1.3516088799073742 1102 92-93 -1.3343372508644222 1102 94-95 -1.4443482737752689 1102 96-97 -1.5318765805704153 1102 98-99 -1.5620792298350281 1102 100 -1.6293802624547418 1103 1 -0.3456203091207293 1103 2 -0.42367901759468296 1103 3 -0.4568030431622496 1103 4 -0.24675474472975623 1103 5 -0.2679626365419665 1103 6 -0.19496905719437763 1103 7 -0.36889922880165926 1103 8 -0.30097724048863483 1103 9 -0.5041791257270134 1103 10-11 -0.49297878804908635 1103 12-13 -0.5790859838985298 1103 14-15 -0.6992124724183455 1103 16-17 -0.7039408814516435 1103 18-19 -0.7269252173864089 1103 20-21 -0.6900939510078103 1103 22-23 -0.8103318653671892 1103 24-25 -0.7031479299410677 1103 26-27 -0.7267563507456813 1103 28-29 -0.8475686037561303 1103 30-31 -0.8051322669017793 1103 32-33 -0.8343072984635427 1103 34-35 -0.7900713414734852 1103 36-37 -0.8649580446109795 1103 38-39 -0.7747365067548273 1103 40-41 -0.8334652550807462 1103 42-43 -0.8737901541919015 1103 44-45 -0.8976762848550024 1103 46-47 -1.019206930036404 1103 48-49 -0.9739851226572682 1103 50-51 -0.8954607746335981 1103 52-53 -0.7179658818559957 1103 54-55 -0.7539107860495591 1103 56-57 -0.7315979117314129 1103 58-59 -0.7131961142549699 1103 60-61 -0.6868354002390831 1103 62-63 -0.7074071035304144 1103 64-65 -0.7024164136706474 1103 66-67 -0.7454831845243319 1103 68-69 -0.6742965509251988 1103 70-71 -0.7236200823268817 1103 72-73 -0.6823449027164301 1103 74-75 -0.8152464492958771 1103 76-77 -0.8547050796442193 1103 78-79 -0.8224604371130226 1103 80-81 -0.8935689323966791 1103 82-83 -0.81797817845721 1103 84-85 -0.8791796758697181 1103 86-87 -1.035682816173452 1103 88-89 -1.009059881783724 1103 90-91 -0.9830153310018872 1103 92-93 -1.0916170019418914 1103 94-95 -1.1540284713099638 1103 96-97 -1.2937907266794078 1103 98-99 -1.3022740723027688 1103 100 -1.3097463499923165 1104 1 -0.4340322530729708 1104 2 -0.46120342217358257 1104 3 -0.41507371989858655 1104 4 -0.3192291602904973 1104 5 -0.36255476656882735 1104 6 -0.34162180364467787 1104 7 -0.3091315889103896 1104 8 -0.4245716670703672 1104 9 -0.4670517720430496 1104 10-11 -0.4749455045166968 1104 12-13 -0.5798751218105949 1104 14-15 -0.72363365361322 1104 16-17 -0.7920861207981602 1104 18-19 -0.7850427931012689 1104 20-21 -0.841409845348494 1104 22-23 -0.7161936434013256 1104 24-25 -0.6947345130421425 1104 26-27 -0.66355875039892 1104 28-29 -0.8698744907213936 1104 30-31 -0.8619618547618728 1104 32-33 -0.8115579157148218 1104 34-35 -0.8637207326192637 1104 36-37 -0.9468234346869266 1104 38-39 -0.9539468135212843 1104 40-41 -0.9938772873939712 1104 42-43 -1.0743776182527256 1104 44-45 -1.0068065944787605 1104 46-47 -1.0820650782695083 1104 48-49 -0.9849154925832408 1104 50-51 -0.9651099328924246 1104 52-53 -0.7603261713398553 1104 54-55 -0.686966466559042 1104 56-57 -0.7209523714868808 1104 58-59 -0.7812446889041595 1104 60-61 -0.8419991601498893 1104 62-63 -0.8264648820001028 1104 64-65 -0.810526577188682 1104 66-67 -0.8248281211494586 1104 68-69 -0.7756203024318182 1104 70-71 -0.8429010968628177 1104 72-73 -0.8830787813960157 1104 74-75 -0.8873148750263589 1104 76-77 -0.9116532666086172 1104 78-79 -0.9571384809876093 1104 80-81 -0.9175271182721865 1104 82-83 -0.9351335260388112 1104 84-85 -0.9420060533072423 1104 86-87 -0.9880048391035601 1104 88-89 -0.9487026676891439 1104 90-91 -0.9798627247465177 1104 92-93 -1.1342231516999917 1104 94-95 -1.1507576713666197 1104 96-97 -1.1357154744501408 1104 98-99 -1.2568024795866144 1104 100 -1.0611152628987242 1105 1 -0.4955810576097903 1105 2 -0.49980811193280417 1105 3 -0.5149978623947327 1105 4 -0.38728943153533635 1105 5 -0.49455207677570456 1105 6 -0.5282620172022234 1105 7 -0.49305524332934425 1105 8 -0.4362917702905662 1105 9 -0.5583360125104946 1105 10-11 -0.6170283501754739 1105 12-13 -0.6780662604792056 1105 14-15 -0.8677338824588361 1105 16-17 -0.8262027091262922 1105 18-19 -0.8354620340798746 1105 20-21 -0.9022210121620589 1105 22-23 -0.8268738906395683 1105 24-25 -0.8186673522701682 1105 26-27 -0.8613963820557089 1105 28-29 -0.776172067031581 1105 30-31 -0.8052964297623717 1105 32-33 -0.9276903203823608 1105 34-35 -0.9553269514160476 1105 36-37 -1.0083129551771606 1105 38-39 -1.146044342143476 1105 40-41 -0.992350608154176 1105 42-43 -0.977432710004571 1105 44-45 -1.0482680390262047 1105 46-47 -1.1067619454412636 1105 48-49 -1.2036935072671255 1105 50-51 -1.1111141728138065 1105 52-53 -0.9278930466971076 1105 54-55 -0.8851412810241328 1105 56-57 -0.8834212023050156 1105 58-59 -0.9716604449318211 1105 60-61 -0.9587333400716744 1105 62-63 -0.9630088874435003 1105 64-65 -0.9572312776773231 1105 66-67 -0.8617814280987091 1105 68-69 -0.9508163366742224 1105 70-71 -0.8777930944445202 1105 72-73 -0.8974188866854078 1105 74-75 -0.8314563994669157 1105 76-77 -0.9416265097933874 1105 78-79 -0.9574094773376913 1105 80-81 -0.9652692588987009 1105 82-83 -1.0383958090145242 1105 84-85 -0.9893779178963449 1105 86-87 -1.0733620170479625 1105 88-89 -1.0632587916199476 1105 90-91 -1.169930810134872 1105 92-93 -1.1157061095306275 1105 94-95 -1.1269381198868622 1105 96-97 -1.0886343931244689 1105 98-99 -1.2129316523916316 1105 100 -1.2274920282767816 1106 1 -0.4111829365586601 1106 2 -0.49805389271876876 1106 3 -0.5038482318312987 1106 4 -0.27772276703642973 1106 5 -0.3406815388207036 1106 6 -0.4063392795530447 1106 7 -0.3881040763233372 1106 8 -0.35645628670468454 1106 9 -0.5377442803524204 1106 10-11 -0.6374387407560462 1106 12-13 -0.5493049026601753 1106 14-15 -0.7235209716055238 1106 16-17 -0.7399729964547248 1106 18-19 -0.8343124535417061 1106 20-21 -0.7978890745335647 1106 22-23 -0.691501194094478 1106 24-25 -0.8640481028825633 1106 26-27 -0.7909297130545312 1106 28-29 -0.8805707883858673 1106 30-31 -0.8848621938065406 1106 32-33 -0.9112411802332545 1106 34-35 -0.8479934980220847 1106 36-37 -0.9224600161095964 1106 38-39 -0.9130568501266723 1106 40-41 -0.8954683492801152 1106 42-43 -0.7363725579611184 1106 44-45 -0.7742012186152252 1106 46-47 -0.8147790774262447 1106 48-49 -0.9055837951958594 1106 50-51 -0.8542817224263644 1106 52-53 -0.7651677871076856 1106 54-55 -0.684873562492939 1106 56-57 -0.7752041360198731 1106 58-59 -0.7117533585198501 1106 60-61 -0.6396956427011631 1106 62-63 -0.7213621891942168 1106 64-65 -0.6784520775145779 1106 66-67 -0.6616239318964574 1106 68-69 -0.6454876456852716 1106 70-71 -0.7313968309981433 1106 72-73 -0.6582290958410297 1106 74-75 -0.556529644789137 1106 76-77 -0.6624013344131541 1106 78-79 -0.6813044609710914 1106 80-81 -0.7144771088629334 1106 82-83 -0.7025656314415158 1106 84-85 -0.7477132105524724 1106 86-87 -0.7335721731768068 1106 88-89 -0.7162604741362877 1106 90-91 -0.8287550163590183 1106 92-93 -0.812853919174497 1106 94-95 -0.8244664550294409 1106 96-97 -0.849503715465584 1106 98-99 -0.8955014334644069 1106 100 -0.8405676314563877 1107 1 -0.5032009541306621 1107 2 -0.49480564413880757 1107 3 -0.48861305787108833 1107 4 -0.38735040689827827 1107 5 -0.40565699751348916 1107 6 -0.3801125136334065 1107 7 -0.4425181978069972 1107 8 -0.38015876839286733 1107 9 -0.5228158583148641 1107 10-11 -0.4817363392737306 1107 12-13 -0.5911041596380571 1107 14-15 -0.6885674799135302 1107 16-17 -0.7346084488463731 1107 18-19 -0.7345272171513528 1107 20-21 -0.7633981861055048 1107 22-23 -0.703327574671107 1107 24-25 -0.7521700054968576 1107 26-27 -0.7594604384198149 1107 28-29 -0.8134254329217541 1107 30-31 -0.7341583918270018 1107 32-33 -0.7736104985819239 1107 34-35 -0.7888964900111688 1107 36-37 -0.6969144410375847 1107 38-39 -0.7566326136549577 1107 40-41 -0.8220228945435011 1107 42-43 -0.7914652706998382 1107 44-45 -0.8299197854820264 1107 46-47 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1.3898165841194512 2310 94-95 1.442491614070157 2310 96-97 1.464249031112839 2310 98-99 1.4237416902040003 2310 100 1.475319680334625 2311 1 0.6066481376726749 2311 2 0.7073311474417672 2311 3 0.7335441241726492 2311 4 0.6337591873162154 2311 5 0.6150983928284219 2311 6 0.6080559670411532 2311 7 0.6590141519278916 2311 8 0.6490177803238595 2311 9 0.8862466933973892 2311 10-11 0.7400708780209655 2311 12-13 0.8579874108271142 2311 14-15 1.0467521668051631 2311 16-17 1.0735500567800216 2311 18-19 1.0617395777785532 2311 20-21 1.1229163152793689 2311 22-23 1.131599462273428 2311 24-25 1.1769272088593183 2311 26-27 1.2214120644034878 2311 28-29 1.2318994991752348 2311 30-31 1.2024158764950386 2311 32-33 1.25545989076776 2311 34-35 1.272934738571756 2311 36-37 1.3161280592298397 2311 38-39 1.3372051272257437 2311 40-41 1.3873157635566429 2311 42-43 1.4615206096581161 2311 44-45 1.4908507750655033 2311 46-47 1.4876620509830474 2311 48-49 1.5253248570176439 2311 50-51 1.4594740627372502 2311 52-53 1.3392583635538031 2311 54-55 1.2480007008316463 2311 56-57 1.2283613636058561 2311 58-59 1.2124514230355814 2311 60-61 1.1835329810927604 2311 62-63 1.1348357916435745 2311 64-65 1.0867701637013454 2311 66-67 1.204366162577358 2311 68-69 1.1565818950408655 2311 70-71 1.1924297950901916 2311 72-73 1.1002847732801584 2311 74-75 1.0931678579710322 2311 76-77 1.0957695906334735 2311 78-79 1.178385709387065 2311 80-81 1.177375381214251 2311 82-83 1.2011174899590316 2311 84-85 1.1345142686798688 2311 86-87 1.2037931255053422 2311 88-89 1.261336063417069 2311 90-91 1.257305585780145 2311 92-93 1.248842959646204 2311 94-95 1.363530189278233 2311 96-97 1.4827506367174976 2311 98-99 1.4740897268549915 2311 100 1.566942295533373 2312 1 0.6275936178063617 2312 2 0.6367927409646583 2312 3 0.700659409258769 2312 4 0.5788809701053026 2312 5 0.6222705886312028 2312 6 0.4157070950644055 2312 7 0.552717430755159 2312 8 0.599483963739452 2312 9 0.8229260610730265 2312 10-11 0.8281588061719134 2312 12-13 0.8967254989619491 2312 14-15 1.083097703747086 2312 16-17 1.0996656385667762 2312 18-19 1.0978532130662018 2312 20-21 1.0845896164271167 2312 22-23 1.153793454409623 2312 24-25 1.154552620315883 2312 26-27 1.1618361984003016 2312 28-29 1.247790196048392 2312 30-31 1.1603910715384131 2312 32-33 1.2052892959771881 2312 34-35 1.251499178145032 2312 36-37 1.30986625167602 2312 38-39 1.3245432430778976 2312 40-41 1.331355335037479 2312 42-43 1.427247062998731 2312 44-45 1.435708054869913 2312 46-47 1.3644378438886307 2312 48-49 1.4921750416702508 2312 50-51 1.4012520319713673 2312 52-53 1.209854640756376 2312 54-55 1.1893625442517788 2312 56-57 1.1903110144492075 2312 58-59 1.1837642330213853 2312 60-61 1.196725523613516 2312 62-63 1.225547655929212 2312 64-65 1.2315834002225614 2312 66-67 1.1915496468262674 2312 68-69 1.1946731075457606 2312 70-71 1.212433071478145 2312 72-73 1.2231918588318749 2312 74-75 1.1869844593887535 2312 76-77 1.1464931270242786 2312 78-79 1.181743953215566 2312 80-81 1.076872331437034 2312 82-83 1.1474612655571548 2312 84-85 1.23227725118414 2312 86-87 1.2342908654155593 2312 88-89 1.3066576180302114 2312 90-91 1.296083283158893 2312 92-93 1.3913636288466122 2312 94-95 1.4327318927674533 2312 96-97 1.446286955611452 2312 98-99 1.3573898603325993 2312 100 1.5861139446543113 2313 1 0.5378806435734802 2313 2 0.5739684763524693 2313 3 0.6377669767791616 2313 4 0.4860053379282476 2313 5 0.50494458864857 2313 6 0.5192956715083881 2313 7 0.5676342094193316 2313 8 0.5928643296735601 2313 9 0.773464770478185 2313 10-11 0.6598051383647743 2313 12-13 0.7855769737878688 2313 14-15 1.0052029365674784 2313 16-17 0.9386290994445119 2313 18-19 0.9718682331951385 2313 20-21 1.010058907995024 2313 22-23 1.0419951791951476 2313 24-25 1.0835094370391118 2313 26-27 1.1055647327324323 2313 28-29 1.123559604279464 2313 30-31 1.1286330031835803 2313 32-33 1.1591077211009946 2313 34-35 1.1937810897498764 2313 36-37 1.2153548770130271 2313 38-39 1.2368993515591669 2313 40-41 1.2225500894164796 2313 42-43 1.2631513099162177 2313 44-45 1.2435059986888106 2313 46-47 1.2401874535554853 2313 48-49 1.2922030928466057 2313 50-51 1.2119520350679167 2313 52-53 1.1098049470369915 2313 54-55 1.1007443322256947 2313 56-57 1.0546579630515964 2313 58-59 1.0890656231856681 2313 60-61 1.0480683660467847 2313 62-63 1.1094041867555475 2313 64-65 1.0502970322696328 2313 66-67 1.0452714510001329 2313 68-69 1.105801684221717 2313 70-71 1.0812292098637286 2313 72-73 1.1023129198208537 2313 74-75 1.130918189726252 2313 76-77 1.1240698552327686 2313 78-79 1.156955817635584 2313 80-81 1.1383677963840562 2313 82-83 1.1693414225937602 2313 84-85 1.1977607112980415 2313 86-87 1.2330335654824154 2313 88-89 1.3246492912424337 2313 90-91 1.3300437140181316 2313 92-93 1.3313507499404622 2313 94-95 1.463348542398915 2313 96-97 1.4613401845433067 2313 98-99 1.4873587455067359 2313 100 1.604170672522958 2314 1 0.52877393390931 2314 2 0.5397451536489335 2314 3 0.6026219298637479 2314 4 0.5038134785218702 2314 5 0.5301106761393868 2314 6 0.5441591205146423 2314 7 0.5687037226977196 2314 8 0.598769767817295 2314 9 0.7357630964501212 2314 10-11 0.746901068026375 2314 12-13 0.5256874723444511 2314 14-15 0.8817587263577877 2314 16-17 0.8857210867472318 2314 18-19 0.9690291036372827 2314 20-21 0.9840738561213556 2314 22-23 0.8778643207891861 2314 24-25 0.9016802738173126 2314 26-27 0.983309157136965 2314 28-29 1.0498360583845923 2314 30-31 1.0170813780128398 2314 32-33 1.0322629222160415 2314 34-35 1.125966610957036 2314 36-37 1.1607350618130994 2314 38-39 1.2174428372468071 2314 40-41 1.1799426668699695 2314 42-43 1.2855139387415093 2314 44-45 1.3206621439984971 2314 46-47 1.3352645179162224 2314 48-49 1.3822423208459327 2314 50-51 1.241676000590985 2314 52-53 1.1071104247082957 2314 54-55 1.0686121941388222 2314 56-57 1.0935595121062391 2314 58-59 1.08167564995167 2314 60-61 1.1227204729445859 2314 62-63 1.1140223585260784 2314 64-65 1.0253114724561811 2314 66-67 1.076773314938137 2314 68-69 1.0964474827214232 2314 70-71 1.1471973599421332 2314 72-73 1.0927191498833508 2314 74-75 1.1366515538977566 2314 76-77 1.1758514975056826 2314 78-79 1.128412844078177 2314 80-81 1.2371315548281565 2314 82-83 1.0387211449252192 2314 84-85 1.022980534406809 2314 86-87 1.2176943695063045 2314 88-89 1.3392737540045303 2314 90-91 1.3837547299206747 2314 92-93 1.4235652680841042 2314 94-95 1.4583522466918915 2314 96-97 1.5033676912361074 2314 98-99 1.587207245336451 2314 100 1.516539325938922 2315 1 0.39399183294994344 2315 2 0.41623895016688905 2315 3 0.4666280774548923 2315 4 0.3815562938893393 2315 5 0.36647201122206496 2315 6 -0.356929171198324 2315 7 0.2392216294080569 2315 8 0.29486250428476524 2315 9 0.5158877413185223 2315 10-11 0.5686372025953403 2315 12-13 0.5943117331158945 2315 14-15 0.7198096290978313 2315 16-17 0.5906214693755203 2315 18-19 0.7246778990863731 2315 20-21 0.7654820997844638 2315 22-23 0.6521692032061566 2315 24-25 0.7584690651623021 2315 26-27 0.7928999490498327 2315 28-29 0.8126828828430988 2315 30-31 0.8597289809867235 2315 32-33 0.9981076815669567 2315 34-35 0.903779147163732 2315 36-37 1.0046978704981413 2315 38-39 1.0585235010756833 2315 40-41 1.0852357187742498 2315 42-43 1.1148535612290686 2315 44-45 1.0502160183394338 2315 46-47 1.1550862514398261 2315 48-49 1.1291446645513048 2315 50-51 1.1003076108119174 2315 52-53 0.9474245876665819 2315 54-55 0.7952489306160828 2315 56-57 0.8254012009252847 2315 58-59 0.7435877449874155 2315 60-61 0.5948058899478212 2315 62-63 0.8666938416013394 2315 64-65 0.9077549726707659 2315 66-67 0.9156708832351796 2315 68-69 0.964260417711742 2315 70-71 0.9605597320662014 2315 72-73 0.9625382979428352 2315 74-75 1.0031492117834304 2315 76-77 0.9686291641136009 2315 78-79 0.8980433909441174 2315 80-81 0.872045381869885 2315 82-83 1.0415375312806532 2315 84-85 0.9642753124016217 2315 86-87 1.1086163664106863 2315 88-89 1.2298193899450744 2315 90-91 1.2772000378183428 2315 92-93 1.369183197069205 2315 94-95 1.4219532056492028 2315 96-97 1.4090586241409149 2315 98-99 1.465160278825298 2315 100 1.5418271028844543 2316 1 0.005492531725401761 2316 2 -0.12290049797490354 2316 3 -0.015648110928459857 2316 4 0.0033719241359690955 2316 5 -0.028324204461199542 2316 6 0.10913228732394487 2316 7 0.06482271001024031 2316 8 0.0846337570577731 2316 9 0.16584617522436673 2316 10-11 0.07155427038935613 2316 12-13 0.15500557005588433 2316 14-15 0.3712965035135767 2316 16-17 0.3888781205319276 2316 18-19 0.3322408935194261 2316 20-21 0.2890279986352695 2316 22-23 0.15615690313035202 2316 24-25 0.25110734654673195 2316 26-27 0.3374424516891352 2316 28-29 0.4583617966832776 2316 30-31 0.45883496115591527 2316 32-33 0.5934960978231985 2316 34-35 0.5596946709081436 2316 36-37 0.6367760385902628 2316 38-39 0.6652769013565845 2316 40-41 0.647501088346246 2316 42-43 0.6740560038039121 2316 44-45 0.5414945507016924 2316 46-47 0.5376391450213305 2316 48-49 0.6375116722343535 2316 50-51 0.3940328019578203 2316 52-53 0.16327317255907303 2316 54-55 0.11501413159832197 2316 56-57 0.26303086969873846 2316 58-59 0.2543787390391046 2316 60-61 0.3828070811216193 2316 62-63 0.41937331411306644 2316 64-65 0.4929355700830911 2316 66-67 0.41903637070372923 2316 68-69 0.45972045554561447 2316 70-71 0.322084876355774 2316 72-73 0.47546468468075176 2316 74-75 0.5087196788119286 2316 76-77 0.5933641148621334 2316 78-79 0.6200481614527789 2316 80-81 0.7209652011649261 2316 82-83 0.692868678179309 2316 84-85 0.7429204844561816 2316 86-87 0.8013653938222163 2316 88-89 0.8673043622033383 2316 90-91 0.8659375011355408 2316 92-93 0.9628835793610051 2316 94-95 1.046620124619153 2316 96-97 1.1195330327978255 2316 98-99 1.13452175095102 2316 100 1.0338667598450044 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 45.0 1 427.0 2 225.0 3 177.0 4 130.0 5 118.0 6 178.0 7 936.0 8 3293.0 9 5783.0 10 7729.0 11 10455.0 12 13908.0 13 17077.0 14 20312.0 15 24102.0 16 29632.0 17 37461.0 18 45602.0 19 54563.0 20 64284.0 21 76430.0 22 83752.0 23 86682.0 24 87152.0 25 91429.0 26 100164.0 27 115041.0 28 134919.0 29 159709.0 30 191213.0 31 234440.0 32 294881.0 33 380549.0 34 522012.0 35 708745.0 36 1033065.0 37 1514877.0 38 1492250.0 39 414050.0 40 651.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.55680579146118 19.05804184228223 12.745644657371338 23.63950770888525 2 15.799865307245012 20.746362904138852 40.612028070477194 22.841743718138943 3 18.398761049930002 27.15422666843253 30.241386488101096 24.205625793536374 4 12.301621779961225 17.15924126614519 39.07779727874607 31.46133967514752 5 12.734653619462218 37.71448587997567 34.889214904874926 14.66164559568719 6 29.166478353225028 37.658686149947826 19.38631836212501 13.788517134702136 7 26.35458355564998 32.91420462750575 23.40644441583008 17.3247674010142 8 25.49794865726851 35.07820282436302 21.14996871738031 18.273879800988155 9 26.960704166478894 15.772597658580164 20.901879037773554 36.36481913716739 10-11 24.478553842416005 26.045724465226446 29.387518762800152 20.088202929557397 12-13 25.241657595687244 24.26487822706714 28.769736829037196 21.723727348208417 14-15 22.686981831694958 25.648465759785317 26.831763532084768 24.832788876434957 16-17 21.86600793788455 28.047920222739858 27.53982982977703 22.546242009598565 18-19 21.509004473457292 27.642422536767093 29.341579198377055 21.506993791398564 20-21 22.598676653063485 26.98456426228479 28.860458006777716 21.55630107787401 22-23 22.387543381443916 26.871794489873942 28.69788020515265 22.0427819235295 24-25 21.996408438580588 26.763946810236444 29.091293752760915 22.14835099842205 26-27 21.981273862408305 26.871059184668184 28.93707938830317 22.210587564620344 28-29 21.739386223096776 27.492126657609457 28.934151626412728 21.83433549288104 30-31 22.59931199559443 26.894663513245913 28.869909975344015 21.636114515815645 32-33 22.42205798194645 27.03683639193903 28.653615007606042 21.88749061850848 34-35 22.496990906498958 27.13450142141422 28.78226947898525 21.586238193101572 36-37 22.270042849276088 27.01263222948489 28.7444708434331 21.972854077805913 38-39 22.16533943613617 27.164330328849818 28.80169688899547 21.868633346018544 40-41 22.20760055083237 26.682185746241142 29.10071321608565 22.00950048684084 42-43 21.99355554539069 26.902758521257287 28.62091563588639 22.48277029746563 44-45 21.735080173872994 27.203526123467704 28.79856814856793 22.26282555409137 46-47 21.720566684527537 26.808189647680674 28.614101165359713 22.857142502432072 48-49 21.894868944958905 26.823040778872887 28.67486757192034 22.607222704247864 50-51 21.756602619188623 27.166171869249357 28.498847900097307 22.57837761146472 52-53 21.59942142007083 27.38224115674272 28.23076256496982 22.787574858216633 54-55 21.444886047203962 27.083190047625067 28.566628537149956 22.905295368021015 56-57 21.880125234417257 26.941464635839846 28.27702611897518 22.90138401076772 58-59 21.64517807307415 27.03362679292417 28.486135994406638 22.835059139595046 60-61 21.839465671689958 27.139144066818343 28.279444967833307 22.74194529365839 62-63 21.82808658025423 26.881979692202318 28.281010408728452 23.008923318814997 64-65 21.537015275853992 26.74243254572286 28.324105989479797 23.396446188943347 66-67 21.65660587822807 26.61743378704429 28.115777291644605 23.610183043083033 68-69 21.374727663301 26.778962811256463 28.28689858519917 23.559410940243364 70-71 21.600565200680506 26.482708295469553 28.409074544716006 23.507651959133934 72-73 21.688986155411577 26.681478871595775 28.21212304049588 23.41741193249677 74-75 21.810356202124296 26.825537283422427 28.003413183218477 23.360693331234796 76-77 21.732659107598533 26.567872402761072 28.049165778768142 23.650302710872253 78-79 21.641473113682615 26.587545706438576 28.262836666578337 23.508144513300472 80-81 21.80639337269875 26.623618616651523 28.139028056469783 23.43095995417994 82-83 22.117490378555697 26.408373720696215 28.303625259056787 23.1705106416913 84-85 22.24329024159418 26.58911117524736 27.850414387242594 23.317184195915868 86-87 21.943238784675096 26.69844544769458 28.114770232661584 23.24354553496874 88-89 22.17457647566389 26.200608272445685 28.08437496431637 23.540440287574054 90-91 22.58780673844834 25.985528277090232 28.157112094631614 23.269552889829807 92-93 22.739091324040807 26.107840767448003 27.98854264377471 23.164525264736483 94-95 22.466204296648886 25.917104928924466 28.109710198597178 23.50698057582947 96-97 22.53055270105874 25.803783757415914 28.03812554079078 23.627538000734564 98-99 22.621682129951175 25.88367507142439 27.784415283279497 23.710227515344947 100 22.93533909997449 25.61090005654097 27.751152697240165 23.70260814624437 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1366.0 1 999.5 2 1542.5 3 2448.0 4 1819.5 5 1318.0 6 1812.5 7 3185.5 8 5048.0 9 6089.5 10 5661.0 11 5094.5 12 5420.5 13 6053.0 14 6751.5 15 7482.5 16 8302.5 17 9196.0 18 10171.0 19 11179.0 20 12321.0 21 14010.0 22 16367.0 23 19861.0 24 24483.0 25 30023.5 26 37506.5 27 46834.5 28 57502.5 29 71586.5 30 88880.0 31 105047.0 32 124258.0 33 144037.5 34 161998.0 35 180592.5 36 201388.5 37 226560.0 38 240802.5 39 243019.5 40 245598.5 41 249948.5 42 256381.5 43 266199.0 44 278796.0 45 295435.0 46 311199.5 47 325571.5 48 342406.0 49 347810.0 50 339581.0 51 330337.5 52 317202.0 53 299508.0 54 279644.5 55 251281.0 56 222442.5 57 193946.0 58 163893.0 59 137797.0 60 110952.5 61 86042.5 62 67258.0 63 52262.5 64 38420.5 65 26680.5 66 19035.0 67 14262.5 68 10568.5 69 7811.0 70 5956.0 71 4487.0 72 3334.0 73 2450.5 74 1820.5 75 1316.5 76 956.5 77 674.5 78 444.5 79 315.5 80 239.0 81 172.5 82 135.0 83 104.0 84 79.0 85 59.0 86 62.0 87 50.0 88 33.5 89 32.0 90 25.5 91 20.0 92 17.5 93 16.0 94 12.0 95 10.5 96 8.5 97 4.0 98 0.5 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2223877352065807 2 0.14875072718717053 3 0.02418579855575168 4 0.023180642227883085 5 0.028107149168177296 6 0.05584201821492178 7 0.061984640218563175 8 0.055283598032772566 9 0.04245234318072165 10-11 0.04484114062658219 12-13 0.042886669989059926 14-15 0.02038233664844645 16-17 0.018806350801047547 18-19 0.018372023992709267 20-21 0.021778387103819494 22-23 0.024917949461236207 24-25 0.02215066722525231 26-27 0.019619162399509185 28-29 0.018787736794975907 30-31 0.01709386224245661 32-33 0.025147522202786442 34-35 0.025253001570525742 36-37 0.02422923123658551 38-39 0.022609812708352776 40-41 0.02374526707872285 42-43 0.02946597161140706 44-45 0.03900254738877759 46-47 0.044623977222413055 48-49 0.04121140944261227 50-51 0.028404973265323548 52-53 0.04410278505240711 54-55 0.042762576615248994 56-57 0.04508932737420406 58-59 0.04843984846709937 60-61 0.024545669339803393 62-63 0.02586726377088988 64-65 0.026543572658159487 66-67 0.03207813713012729 68-69 0.03639038187005736 70-71 0.04040480251284118 72-73 0.0447232519214618 74-75 0.025302638920050115 76-77 0.018645029415093328 78-79 0.0181114279077063 80-81 0.021275808939885198 82-83 0.01955091104391317 84-85 0.011770256505967403 86-87 0.013402084371581227 88-89 0.019011104867835592 90-91 0.019401998995340047 92-93 0.020047284539156923 94-95 0.02374526707872285 96-97 0.024309891929562618 98-99 0.0294845856174787 100 0.029075077483902603 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8058448.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.1421132429382 #Duplication Level Percentage of deduplicated Percentage of total 1 78.76481120975173 52.09671062594047 2 13.624016931467295 18.022425414096343 3 3.838796879681793 7.61718413797653 4 1.4617546475865317 3.8673416573623838 5 0.7149001184995217 2.364250229759265 6 0.39326478737037784 1.5606818460426926 7 0.25390042246443095 1.1755457346550569 8 0.17382588217698972 0.9197768946803268 9 0.1315857931366216 0.7833026187723839 >10 0.600524048407854 6.858417382064644 >50 0.027901706252328212 1.261399043686542 >100 0.013248726970667573 1.6247220830917537 >500 0.0010237702724522739 0.47382864992322304 >1k 3.7831456823298566E-4 0.5126232179229958 >5k 0.0 0.0 >10k+ 6.676139311019635E-5 0.8617904640252437 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25725 0.3192302041286362 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20060 0.2489313078647402 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12764 0.15839278233228035 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.240933738109373E-5 2 0.0 0.0 0.0 0.0 1.240933738109373E-5 3 0.0 0.0 0.0 0.0 1.240933738109373E-5 4 0.0 0.0 0.0 1.240933738109373E-5 2.481867476218746E-5 5 0.0 0.0 0.0 1.240933738109373E-5 2.481867476218746E-5 6 0.0 0.0 0.0 1.240933738109373E-5 4.963734952437492E-5 7 0.0 0.0 0.0 1.240933738109373E-5 4.963734952437492E-5 8 0.0 0.0 0.0 2.481867476218746E-5 4.963734952437492E-5 9 0.0 0.0 0.0 6.204668690546864E-5 6.204668690546864E-5 10-11 1.240933738109373E-5 0.0 0.0 1.240933738109373E-4 6.825135559601552E-5 12-13 1.240933738109373E-5 0.0 0.0 1.6132138595421848E-4 1.3029804250148415E-4 14-15 1.240933738109373E-5 0.0 0.0 1.7993539202585909E-4 1.8614006071640592E-4 16-17 1.240933738109373E-5 0.0 0.0 2.7300542238406207E-4 2.3577741024078086E-4 18-19 1.240933738109373E-5 6.204668690546865E-6 0.0 3.846894588139056E-4 2.605960850029683E-4 20-21 1.240933738109373E-5 1.240933738109373E-5 0.0 6.142622003641395E-4 2.73005422384062E-4 22-23 1.240933738109373E-5 1.240933738109373E-5 0.0 0.0018179679263302315 2.8541475976515574E-4 24-25 1.240933738109373E-5 1.240933738109373E-5 0.0 0.004126104679213665 2.978240971462495E-4 26-27 1.240933738109373E-5 1.240933738109373E-5 0.0 0.008202572008902955 3.2264277190843696E-4 28-29 1.240933738109373E-5 1.240933738109373E-5 0.0 0.021263399602504104 3.350521092895307E-4 30-31 1.240933738109373E-5 1.240933738109373E-5 0.0 0.06581292080063059 3.350521092895307E-4 32-33 1.240933738109373E-5 1.240933738109373E-5 0.0 0.1682085682007255 3.474614466706244E-4 34-35 1.240933738109373E-5 1.240933738109373E-5 0.0 0.31358395562023855 3.536661153611713E-4 36-37 1.240933738109373E-5 1.240933738109373E-5 0.0 0.5409416304479473 3.5987078405171817E-4 38-39 1.240933738109373E-5 1.240933738109373E-5 0.0 0.8918652822478969 3.66075452742265E-4 40-41 1.240933738109373E-5 1.240933738109373E-5 0.0 1.2882877695556265 3.722801214328119E-4 42-43 1.240933738109373E-5 1.240933738109373E-5 0.0 1.6640425054551446 3.722801214328119E-4 44-45 1.240933738109373E-5 1.240933738109373E-5 0.0 2.0964334571619743 3.722801214328119E-4 46-47 1.240933738109373E-5 1.240933738109373E-5 0.0 2.5921430528558354 3.9089412750445245E-4 48-49 1.240933738109373E-5 1.240933738109373E-5 0.0 3.1132297434940326 4.219174709571868E-4 50-51 1.240933738109373E-5 1.240933738109373E-5 0.0 3.653668795778046 4.59145483100468E-4 52-53 1.240933738109373E-5 1.240933738109373E-5 0.0 4.1779757094666365 4.653501517910149E-4 54-55 1.240933738109373E-5 1.240933738109373E-5 0.0 4.7504122381878 4.8396415786265543E-4 56-57 1.240933738109373E-5 1.240933738109373E-5 0.0 5.380651460430098 4.8396415786265543E-4 58-59 1.240933738109373E-5 1.240933738109373E-5 0.0 6.061011996354633 4.8396415786265543E-4 60-61 1.240933738109373E-5 1.240933738109373E-5 0.0 6.740224668571417 4.8396415786265543E-4 62-63 1.240933738109373E-5 1.240933738109373E-5 0.0 7.440049250178198 5.087828326248429E-4 64-65 1.8614006071640595E-5 1.240933738109373E-5 0.0 8.104705769646959 5.087828326248429E-4 66-67 2.481867476218746E-5 1.240933738109373E-5 0.0 8.821121635332263 5.087828326248429E-4 68-69 2.481867476218746E-5 1.240933738109373E-5 0.0 9.598033020750398 5.087828326248429E-4 70-71 2.481867476218746E-5 1.240933738109373E-5 0.0 10.420430832338932 5.336015073870304E-4 72-73 2.481867476218746E-5 1.240933738109373E-5 0.0 11.184479939561562 5.770341882208584E-4 74-75 2.481867476218746E-5 1.240933738109373E-5 0.0 11.92886645170385 5.832388569114053E-4 76-77 2.481867476218746E-5 1.240933738109373E-5 0.0 12.698921678218932 6.080575316735927E-4 78-79 2.481867476218746E-5 1.240933738109373E-5 0.0 13.52043842685341 6.204668690546865E-4 80-81 2.481867476218746E-5 1.240933738109373E-5 0.0 14.349655169332856 6.576948811979676E-4 82-83 2.481867476218746E-5 1.240933738109373E-5 0.0 15.173399394027236 6.763088872696083E-4 84-85 2.481867476218746E-5 1.240933738109373E-5 0.0 15.979311400904988 6.88718224650702E-4 86-87 2.481867476218746E-5 1.8614006071640595E-5 0.0 16.78193493337675 7.073322307223426E-4 88 2.481867476218746E-5 2.481867476218746E-5 0.0 17.38749198356805 7.3215090548453E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 8170 0.0 47.810413 2 ACCTGGG 9565 0.0 40.251278 3 GGTATCA 17815 0.0 36.307312 1 GTATCAA 28165 0.0 33.302044 1 GTACCTG 12660 0.0 31.927359 1 ATAACGC 925 0.0 28.459488 3 ATCAACG 32770 0.0 27.972994 3 CAACGCA 32820 0.0 27.887236 5 GTACATG 25215 0.0 27.781866 1 TATCAAC 33870 0.0 27.331003 2 TCAACGC 33930 0.0 27.154861 4 TACATGG 25375 0.0 27.096338 2 TAACGCA 1010 0.0 26.994919 4 AACGCAG 34720 0.0 26.632595 6 CCTGGGG 9425 0.0 26.434427 4 ACATGGG 25410 0.0 25.84476 3 ACGCAGA 37615 0.0 24.320698 7 CATGGGG 18915 0.0 24.057163 4 CGCAGAG 38200 0.0 23.91029 8 ATGGGGG 14535 0.0 22.574518 5 >>END_MODULE