FastQCFastQC Report
Mon 6 Jun 2016
ERR1211439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211439_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8058448
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226800.2814437718032058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188990.2345240671652904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121830.1511829573138649No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG82350.046.1089252
ACCTGGG94500.039.3364683
GGTATCA178450.035.9120561
GTATCAA274000.031.5739021
GTACCTG125150.031.06961
CCTGGGG87900.029.24524
GTACATG244050.026.1433961
ATCAACG326250.026.1298833
CAACGCA329000.026.0794835
TCAACGC339250.025.4059584
TACATGG247450.025.3151172
TATCAAC341200.025.1770272
AACGCAG349800.024.8776826
TGGGGGG143450.024.5668496
TAACGCA8300.024.3470574
ACATGGG245550.024.1529483
CTGGGGG64350.023.4396235
CATGGGG182100.022.5299594
ACGCAGA383400.022.354327
CGCAGAG386750.022.0756448
Produced by FastQC (version 0.11.5)