FastQCFastQC Report
Mon 6 Jun 2016
ERR1211438_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211438_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9345520
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297290.31810963969902156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225260.24103527679572673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140600.15044641710680626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140550.050.2439961
TACCTGG92550.048.366782
GTATCAA254850.042.072761
ACCTGGG114500.038.6312333
CAACGCA311800.033.9955865
ATCAACG311100.033.9816063
TATCAAC323450.032.9321822
TCAACGC324900.032.6538164
GTACCTG141300.032.373561
AACGCAG334800.031.9841166
ACGCAGA367500.028.6909547
CGCAGAG373350.028.1643418
CCTGGGG110050.027.0801664
GTACATG289850.026.232741
TACATGG290650.025.215142
GTACTAG31300.025.1353861
GCAGAGT409600.024.7877249
ACATGGG296750.023.9347113
CATGGGG216050.022.6707344
GTGGTAT62600.021.8993931
Produced by FastQC (version 0.11.5)