FastQCFastQC Report
Mon 6 Jun 2016
ERR1211438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211438_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9345520
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271910.2909522423578356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223430.2390771193042228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142400.15237247365582654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142050.053.0559121
TACCTGG94950.047.612072
GTATCAA258300.040.954311
ACCTGGG110600.039.8560183
ATCAACG317500.032.7406773
CAACGCA319200.032.6500975
GTACCTG144100.032.0071141
TATCAAC332050.031.630862
TCAACGC332400.031.4011384
AACGCAG342350.030.90766
CCTGGGG105550.029.0745354
ACGCAGA381600.027.2115617
TAACGCA9850.027.1954654
CGCAGAG389500.026.502688
ATAACGC9650.026.2973923
GTACATG283950.025.6809711
TACATGG291900.024.2453142
CTGGGGG74250.024.0476675
GCAGAGT427850.023.6328249
TGGGGGG146900.023.5089266
Produced by FastQC (version 0.11.5)