##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211438_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9345520 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08577799844203 33.0 31.0 34.0 30.0 34.0 2 32.22354111916726 33.0 31.0 34.0 30.0 34.0 3 32.3037856641471 34.0 31.0 34.0 30.0 34.0 4 35.94023350225562 37.0 35.0 37.0 35.0 37.0 5 35.80319746787766 37.0 35.0 37.0 35.0 37.0 6 35.83636812076803 37.0 35.0 37.0 35.0 37.0 7 35.768963417765946 37.0 35.0 37.0 33.0 37.0 8 35.752796099093466 37.0 35.0 37.0 33.0 37.0 9 37.34665914791258 39.0 37.0 39.0 34.0 39.0 10-11 37.32613059519427 39.0 37.0 39.0 34.0 39.0 12-13 37.24425890694151 39.0 37.0 39.0 33.5 39.0 14-15 38.46665214990712 40.0 38.0 41.0 34.0 41.0 16-17 38.46100195601743 40.0 38.0 41.0 34.0 41.0 18-19 38.46738731499157 40.0 38.0 41.0 34.0 41.0 20-21 38.42954110632688 40.0 38.0 41.0 34.0 41.0 22-23 38.326820444448245 40.0 38.0 41.0 34.0 41.0 24-25 38.21391351150069 40.0 38.0 41.0 33.5 41.0 26-27 38.09672982348762 40.0 37.5 41.0 33.0 41.0 28-29 38.02824042963901 40.0 37.0 41.0 33.0 41.0 30-31 38.070843248957786 40.0 37.0 41.0 33.0 41.0 32-33 37.91919229748585 40.0 37.0 41.0 33.0 41.0 34-35 37.81171877006309 40.0 37.0 41.0 33.0 41.0 36-37 37.672041202629714 40.0 37.0 41.0 33.0 41.0 38-39 37.55946785197614 40.0 37.0 41.0 32.0 41.0 40-41 37.498952171735766 40.0 37.0 41.0 32.0 41.0 42-43 37.3749039646804 40.0 36.0 41.0 32.0 41.0 44-45 37.226745328242835 40.0 36.0 41.0 31.0 41.0 46-47 37.01527748054683 39.5 36.0 41.0 31.0 41.0 48-49 36.81015711271283 39.0 36.0 41.0 30.5 41.0 50-51 36.63434051823762 39.0 35.0 41.0 30.0 41.0 52-53 36.436582983076384 39.0 35.0 40.5 30.0 41.0 54-55 36.244628977306775 39.0 35.0 40.0 29.0 41.0 56-57 36.02401492907832 38.0 35.0 40.0 29.0 41.0 58-59 35.767304280553674 38.0 34.5 40.0 28.0 41.0 60-61 35.52008309863977 38.0 34.0 40.0 28.0 41.0 62-63 35.23833414299044 37.0 34.0 40.0 28.0 41.0 64-65 34.897032481873666 37.0 34.0 40.0 27.0 41.0 66-67 34.550590175827566 36.0 33.5 39.0 26.0 41.0 68-69 34.18656447153288 36.0 33.0 39.0 26.0 40.0 70-71 33.81673973197853 35.0 33.0 38.5 26.0 40.0 72-73 33.46518529734033 35.0 32.5 38.0 26.0 40.0 74-75 33.09889904467595 35.0 32.0 37.0 25.0 39.0 76-77 32.073227439457625 34.0 30.5 36.0 24.5 39.0 78-79 32.54542433165838 35.0 32.0 36.0 25.0 39.0 80-81 32.42814150523459 35.0 32.0 36.0 25.0 37.5 82-83 32.15415525299823 35.0 32.0 35.0 25.0 37.0 84-85 31.87265053201962 34.0 31.0 35.0 24.0 37.0 86-87 31.612522203151883 34.0 31.0 35.0 24.0 36.0 88-89 31.378185376522655 34.0 31.0 35.0 23.0 36.0 90-91 31.152676630085857 34.0 31.0 35.0 23.0 35.5 92-93 30.97012456235715 34.0 30.5 35.0 21.5 35.0 94-95 30.799986303597873 34.0 30.0 35.0 20.0 35.0 96-97 30.625408966007242 34.0 30.0 35.0 20.0 35.0 98-99 30.432999019851223 34.0 30.0 35.0 19.5 35.0 100 28.643076040712554 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.25421836732797587 1101 2 -0.24459053797973596 1101 3 -0.2950456277524971 1101 4 -0.16438980150954308 1101 5 -0.18523073663664036 1101 6 -0.284385648483088 1101 7 -0.2505684090873359 1101 8 -0.33139490015078366 1101 9 -0.4646986219443576 1101 10-11 -0.39018943064508704 1101 12-13 -0.4479813883705148 1101 14-15 -0.5852159978831821 1101 16-17 -0.6363299931676138 1101 18-19 -0.6053677599755574 1101 20-21 -0.6046945249640956 1101 22-23 -0.6421201687126583 1101 24-25 -0.7172474712170853 1101 26-27 -0.6469106498295218 1101 28-29 -0.6423898489055944 1101 30-31 -0.6960094017010618 1101 32-33 -0.5793883304578955 1101 34-35 -0.5923455558985324 1101 36-37 -0.5716352246112208 1101 38-39 -0.6316289560256578 1101 40-41 -0.6752331343050031 1101 42-43 -0.6517333507282501 1101 44-45 -0.6540077166023579 1101 46-47 -0.7414362943654211 1101 48-49 -0.698527469308253 1101 50-51 -0.8069080980185319 1101 52-53 -0.785037831393609 1101 54-55 -0.7492649047956945 1101 56-57 -0.7344797683899955 1101 58-59 -0.6929541435352533 1101 60-61 -0.732439055733316 1101 62-63 -0.8235662668395989 1101 64-65 -0.7168471646861647 1101 66-67 -0.7347478213320926 1101 68-69 -0.815731929571264 1101 70-71 -0.8220143289336903 1101 72-73 -0.853768485006519 1101 74-75 -0.8506123984565761 1101 76-77 -0.7376202112774273 1101 78-79 -0.6365968949045282 1101 80-81 -0.5331332289672375 1101 82-83 -0.6345504839171809 1101 84-85 -0.7370398575203687 1101 86-87 -0.8092678932324162 1101 88-89 -0.824764670601045 1101 90-91 -0.9289430321766652 1101 92-93 -1.0703193335533214 1101 94-95 -1.0731103218959284 1101 96-97 -1.0495003992342866 1101 98-99 -1.1328691972820053 1101 100 -1.1099964321711049 1102 1 -0.2872427882297117 1102 2 -0.23061820366607577 1102 3 -0.29002292698307386 1102 4 -0.1779116831023515 1102 5 -0.17584274132730116 1102 6 -0.29305038349751555 1102 7 -0.2583858868912756 1102 8 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-1.0217441520758896 1102 72-73 -1.13709587266262 1102 74-75 -1.1072538549061122 1102 76-77 -0.8870881611190278 1102 78-79 -0.6932806022081053 1102 80-81 -0.6660476623790892 1102 82-83 -0.7363988722638624 1102 84-85 -0.782338673501318 1102 86-87 -0.8310250655134155 1102 88-89 -0.8309579826683695 1102 90-91 -0.7295080660890001 1102 92-93 -0.7908715056755717 1102 94-95 -0.9050266583983699 1102 96-97 -1.009010366008713 1102 98-99 -1.0789440515849122 1102 100 -0.9793075603062427 1103 1 -0.33236610031285707 1103 2 -0.2622294998775736 1103 3 -0.28901777485441826 1103 4 -0.17922739386194309 1103 5 -0.16647279893864209 1103 6 -0.3230848019053951 1103 7 -0.28800208800959837 1103 8 -0.35257483262174105 1103 9 -0.4629239975678203 1103 10-11 -0.4543058994527982 1103 12-13 -0.5402539331469995 1103 14-15 -0.6801282368419592 1103 16-17 -0.7220284296911714 1103 18-19 -0.6789649398086866 1103 20-21 -0.6369833568506138 1103 22-23 -0.7801476356301507 1103 24-25 -0.708619621772435 1103 26-27 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-0.8976802176819305 1104 48-49 -0.9244402837550894 1104 50-51 -0.9349830311647622 1104 52-53 -0.837356736261178 1104 54-55 -0.7431112757618195 1104 56-57 -0.8528540088378307 1104 58-59 -0.9611984104051601 1104 60-61 -0.9692498053229244 1104 62-63 -0.9985656071008151 1104 64-65 -0.8866754694285675 1104 66-67 -0.9314861518910718 1104 68-69 -0.9323391132676662 1104 70-71 -0.982364525478296 1104 72-73 -0.9204712711129304 1104 74-75 -0.8893296694051287 1104 76-77 -0.8352005642245857 1104 78-79 -0.770553338215727 1104 80-81 -0.8424537466479336 1104 82-83 -0.9059668856882936 1104 84-85 -0.8207671275765058 1104 86-87 -0.8443080952825675 1104 88-89 -0.8718776824313643 1104 90-91 -0.8148630509423143 1104 92-93 -0.9681108518363146 1104 94-95 -0.9065879703917652 1104 96-97 -0.9971297872490688 1104 98-99 -0.90121713019591 1104 100 -1.0840489415080974 1105 1 -0.4169609105344634 1105 2 -0.38829679281721496 1105 3 -0.41376633664865636 1105 4 -0.27182430247250977 1105 5 -0.2782067168055704 1105 6 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2311 30-31 0.8609315472511128 2311 32-33 0.9978261974372771 2311 34-35 0.925260441813009 2311 36-37 1.0505886085229221 2311 38-39 1.0564550278994957 2311 40-41 1.0203898734948993 2311 42-43 1.0821925398890784 2311 44-45 1.0262814585876114 2311 46-47 1.077708684370542 2311 48-49 1.0459109277223533 2311 50-51 1.1234025901445932 2311 52-53 1.1014179695005737 2311 54-55 1.0787173947690292 2311 56-57 1.1125021305065843 2311 58-59 1.184198701397392 2311 60-61 1.2004632322547906 2311 62-63 1.18013366270322 2311 64-65 1.223021111818234 2311 66-67 1.2670896884157017 2311 68-69 1.282594579539655 2311 70-71 1.2429322229625868 2311 72-73 1.2278864193323145 2311 74-75 1.1409415149859328 2311 76-77 1.0642570941779077 2311 78-79 1.0289122366062102 2311 80-81 1.0551296320369161 2311 82-83 1.0430248351970448 2311 84-85 1.066247283069199 2311 86-87 1.0896483040343448 2311 88-89 1.1194053773342496 2311 90-91 1.1797829853584254 2311 92-93 1.220536700137572 2311 94-95 1.2553855011188624 2311 96-97 1.2558177800534303 2311 98-99 1.2961286177901954 2311 100 1.4119875433859441 2312 1 0.11499542776583382 2312 2 0.1577667022832543 2312 3 0.3377670121892251 2312 4 0.22952817276713944 2312 5 0.2960055290100314 2312 6 0.4075630128265786 2312 7 0.439686559493353 2312 8 0.3016455424696147 2312 9 0.40934501870657414 2312 10-11 0.26005180099767955 2312 12-13 0.47246188606364825 2312 14-15 0.7777188641320336 2312 16-17 0.8363764262798838 2312 18-19 0.8095524176741904 2312 20-21 0.8338924008619912 2312 22-23 0.47318167881461903 2312 24-25 0.4142548435380249 2312 26-27 0.7543383961862276 2312 28-29 0.8642274635973948 2312 30-31 0.8169219017474916 2312 32-33 0.6765672083531697 2312 34-35 0.8521111004874626 2312 36-37 0.935631553513673 2312 38-39 0.9070487567801635 2312 40-41 0.9671983548528473 2312 42-43 0.8306438109785645 2312 44-45 0.8088273151031586 2312 46-47 0.9995470105157054 2312 48-49 1.0892898865504748 2312 50-51 1.061891472298143 2312 52-53 1.10127076837788 2312 54-55 1.102340754217657 2312 56-57 1.1066074368434258 2312 58-59 1.1211583863956065 2312 60-61 1.105715052621008 2312 62-63 1.077193677585818 2312 64-65 1.1109725722563155 2312 66-67 1.1253492415662762 2312 68-69 1.1951845449926566 2312 70-71 1.0062146703810413 2312 72-73 1.0997253092123458 2312 74-75 1.188099371029267 2312 76-77 1.1186658987880627 2312 78-79 0.9774467000400406 2312 80-81 0.8426238288838519 2312 82-83 0.9066262404885919 2312 84-85 0.753565586346614 2312 86-87 0.9388143494872985 2312 88-89 0.5784151372428852 2312 90-91 0.9320756287510186 2312 92-93 1.126774492790588 2312 94-95 1.226365507764303 2312 96-97 1.2522828888443094 2312 98-99 1.271022884341285 2312 100 1.3328475242454871 2313 1 -0.3928093365630545 2313 2 -0.03863588378484195 2313 3 0.22073115103125218 2313 4 0.05286660219946526 2313 5 0.21657334329474764 2313 6 0.3181947852971234 2313 7 0.10012594622279636 2313 8 0.04135410595088729 2313 9 0.24562427277573562 2313 10-11 0.3608064398668134 2313 12-13 0.5333096417478345 2313 14-15 0.6138885393204347 2313 16-17 0.7395435994361677 2313 18-19 0.6147598157707961 2313 20-21 0.7899587827744199 2313 22-23 0.848411919004171 2313 24-25 0.7228650150824549 2313 26-27 0.9035376775337056 2313 28-29 0.7983263079480096 2313 30-31 0.783208235872408 2313 32-33 0.9196624039272976 2313 34-35 0.9721441011336083 2313 36-37 0.8477864170756746 2313 38-39 0.6385888992636453 2313 40-41 0.7224173441191368 2313 42-43 0.7609349886495451 2313 44-45 0.936577169521648 2313 46-47 0.9962653419993828 2313 48-49 1.0582502481689247 2313 50-51 0.9810955740744731 2313 52-53 0.9492611086356888 2313 54-55 1.044306982888969 2313 56-57 1.071209974495602 2313 58-59 0.9548433755215626 2313 60-61 0.9406123201818346 2313 62-63 0.9993862661723867 2313 64-65 1.0947799849721207 2313 66-67 1.075528481423234 2313 68-69 1.0649499800921163 2313 70-71 1.0125794435140065 2313 72-73 1.0998298495158352 2313 74-75 0.6929739788301532 2313 76-77 0.8530729184785635 2313 78-79 0.7265638419962173 2313 80-81 0.8158694801195523 2313 82-83 0.7924085312976814 2313 84-85 0.8451500797058706 2313 86-87 0.9759460266400133 2313 88-89 0.9555206561211058 2313 90-91 1.0103039038140373 2313 92-93 0.8736374289999169 2313 94-95 0.9021780739922853 2313 96-97 1.175706803153009 2313 98-99 1.2319377125241715 2313 100 1.1808937691053636 2314 1 -0.32554739407731503 2314 2 -0.13199819279535774 2314 3 0.13193094249389503 2314 4 -0.06722580987759841 2314 5 0.2113462187764199 2314 6 0.2493296741601938 2314 7 0.14550221757888693 2314 8 0.21436368956526053 2314 9 0.28508782036032443 2314 10-11 0.43621426280925135 2314 12-13 0.4921390985170717 2314 14-15 0.5662582531424007 2314 16-17 0.784921670533528 2314 18-19 0.8272957650131261 2314 20-21 0.48166442444929913 2314 22-23 0.6917208487890463 2314 24-25 0.8891195174220812 2314 26-27 0.8586128190106592 2314 28-29 0.781733180752795 2314 30-31 0.769208748036327 2314 32-33 0.8304709049179237 2314 34-35 0.8942352665636832 2314 36-37 0.8011776608958812 2314 38-39 0.8536688338681202 2314 40-41 0.9465440357816135 2314 42-43 0.7188484321289295 2314 44-45 0.7738934560200832 2314 46-47 0.9313546553778096 2314 48-49 0.7875376625421069 2314 50-51 0.911216595593153 2314 52-53 1.0071601447540033 2314 54-55 1.0582435505129695 2314 56-57 0.9144476298109154 2314 58-59 0.8447851104306494 2314 60-61 0.9831674853166632 2314 62-63 0.6921102391787244 2314 64-65 0.8620221035829019 2314 66-67 0.9123011517501212 2314 68-69 1.0150077258515822 2314 70-71 1.035131207530803 2314 72-73 1.0184634447657572 2314 74-75 1.0397061186693932 2314 76-77 0.8058183538317287 2314 78-79 0.8257896058787182 2314 80-81 0.835086873711937 2314 82-83 0.8888584182645047 2314 84-85 0.8065225080092127 2314 86-87 0.8866156096105691 2314 88-89 0.9510041150370903 2314 90-91 0.9991353696918246 2314 92-93 1.0718806347487266 2314 94-95 1.1439439511996987 2314 96-97 1.2305350169847777 2314 98-99 1.2372025372994742 2314 100 1.2893153856420874 2315 1 -0.04983740542584414 2315 2 0.10425376107862405 2315 3 0.13154187095145176 2315 4 -0.04478338954601924 2315 5 0.26209006565499493 2315 6 0.3836839370606313 2315 7 0.4307871619944592 2315 8 0.404405672306936 2315 9 0.6411856712449477 2315 10-11 0.6246535457496591 2315 12-13 0.7001044988884857 2315 14-15 0.7813948028978146 2315 16-17 0.7950294097723756 2315 18-19 0.8530374767429905 2315 20-21 0.8215998079147084 2315 22-23 0.9497532660645049 2315 24-25 0.9137357746268364 2315 26-27 0.9552010460318314 2315 28-29 0.9872895433256232 2315 30-31 0.9606363875424435 2315 32-33 0.9747306762343797 2315 34-35 0.9793471605221029 2315 36-37 1.0262270187210092 2315 38-39 1.0045999107811596 2315 40-41 1.0129382489568073 2315 42-43 1.0508963268601406 2315 44-45 0.9469811718221806 2315 46-47 0.9699839057228488 2315 48-49 1.0736919957138724 2315 50-51 0.9622152308012346 2315 52-53 0.9798175581363111 2315 54-55 0.862022897554084 2315 56-57 1.0421032134133625 2315 58-59 1.0753384071489194 2315 60-61 1.126863145477877 2315 62-63 1.1232138263745455 2315 64-65 1.1434586705654937 2315 66-67 1.1745084538857 2315 68-69 1.0438470152156967 2315 70-71 0.6865332641839004 2315 72-73 0.9262784601047542 2315 74-75 1.0666620061320913 2315 76-77 1.0526959573748833 2315 78-79 0.9212198340853561 2315 80-81 0.8972639552811827 2315 82-83 0.8901783477303837 2315 84-85 0.8899175234163472 2315 86-87 0.8637976220958912 2315 88-89 0.9282538013952966 2315 90-91 0.9529722988345704 2315 92-93 1.0730074275436792 2315 94-95 1.110277765823529 2315 96-97 1.1218972515937047 2315 98-99 1.0651617134994886 2315 100 0.9311232641096652 2316 1 0.4969031543436131 2316 2 0.493443117525473 2316 3 0.46271327280221186 2316 4 0.3007197653985614 2316 5 0.38433811536129525 2316 6 0.42081397596084713 2316 7 0.44688759796910205 2316 8 0.46602394967278116 2316 9 0.49321540458254987 2316 10-11 0.4162513058627795 2316 12-13 0.6120737415749957 2316 14-15 0.7523188611388534 2316 16-17 0.7552251470501048 2316 18-19 0.7946957712797413 2316 20-21 0.8321856190261698 2316 22-23 0.8515683629467574 2316 24-25 0.9293818213010638 2316 26-27 0.9376887565098997 2316 28-29 0.9103006090273169 2316 30-31 0.809888284403435 2316 32-33 0.7808656110193013 2316 34-35 0.6537097340222573 2316 36-37 0.6378444583707363 2316 38-39 0.5872617530079722 2316 40-41 0.7279104322533243 2316 42-43 0.8423517507022353 2316 44-45 0.7598082509128474 2316 46-47 0.8442902390378677 2316 48-49 0.5388576084179348 2316 50-51 0.6125605660657953 2316 52-53 0.7354744211596085 2316 54-55 0.850248797442319 2316 56-57 0.8973084946173131 2316 58-59 0.8252933063241272 2316 60-61 0.87799935268567 2316 62-63 0.9084442043460186 2316 64-65 0.90148206654289 2316 66-67 0.9129721293070858 2316 68-69 0.849686877703455 2316 70-71 0.7155315323001545 2316 72-73 0.7670298250537542 2316 74-75 0.8311366841623169 2316 76-77 0.7077817923348455 2316 78-79 0.6927442102660848 2316 80-81 0.6945246160112859 2316 82-83 0.5763552317162066 2316 84-85 0.6477660253987843 2316 86-87 0.6487468217826624 2316 88-89 0.5580468658906845 2316 90-91 0.6317740659174902 2316 92-93 0.6974557877030456 2316 94-95 0.6018169461839626 2316 96-97 0.7921643668133278 2316 98-99 0.84520257593352 2316 100 0.45509448833442434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 3.0 11 6.0 12 52.0 13 242.0 14 927.0 15 2787.0 16 6852.0 17 13499.0 18 22154.0 19 32000.0 20 42795.0 21 56855.0 22 69190.0 23 73925.0 24 76636.0 25 84046.0 26 98709.0 27 120238.0 28 147729.0 29 180202.0 30 220485.0 31 272356.0 32 343637.0 33 444945.0 34 618457.0 35 857277.0 36 1308268.0 37 2034516.0 38 1907074.0 39 309611.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.56511418669414 19.957908791962204 13.492546785733097 23.984430235610557 2 15.37216927171193 21.093520862779265 40.23001825794801 23.3042916075608 3 17.784812165060067 27.083107957942197 30.857248270534786 24.27483160646295 4 12.152578337731404 17.625846424002734 38.977141053668454 31.24443418459741 5 12.627743224962265 37.79526927119348 35.16921248900762 14.407775014836629 6 29.372576378842485 38.16828812093923 18.83793518177694 13.621200318441348 7 26.242546816367184 32.89678468884981 23.367773783818805 17.492894710964194 8 25.590223893285724 35.47598790816394 20.86281505741974 18.070973141130594 9 26.51182340339085 15.641624295195426 20.752042653216513 37.09450964819721 10-11 24.382038787007453 26.283263650574735 29.190684150114137 20.14401341230368 12-13 25.02441283990185 24.274318569851648 28.833864498519713 21.86740409172679 14-15 22.490088028302768 25.689472289227016 26.62031431754926 25.200125364920957 16-17 21.825709788673393 28.234418012798766 27.332637419860582 22.607234778667255 18-19 21.509364914953903 27.636594860425102 29.27985815663548 21.574182067985518 20-21 22.486783371088507 26.965662739846913 28.903629049581358 21.643924839483226 22-23 22.338896069988614 26.891473133651207 28.7087449387514 22.06088585760878 24-25 21.941653160627233 26.856274999637524 29.066448536973983 22.135623302761257 26-27 21.968366117767154 27.048427227277678 28.785409689725583 22.19779696522959 28-29 21.81298676840618 27.451536498836067 28.824779156882478 21.91069757587528 30-31 22.471684862879158 27.049573786924814 28.734839001211604 21.74390234898442 32-33 22.318619532676852 27.203158580329738 28.50211971107633 21.976102175917077 34-35 22.360634542811948 27.097342300784938 28.822283489343302 21.719739667059812 36-37 22.20294694000926 27.0473132629212 28.781692631301333 21.968047165768212 38-39 22.14387536842592 27.000507457723494 28.874381925536376 21.981235248314213 40-41 22.15422636137261 26.626703419457282 29.138628004365952 22.080442214804158 42-43 21.966527263810818 26.840118232226402 28.76481072860187 22.42854377536091 44-45 21.704920450229668 27.19097220624458 28.86258055810098 22.241526785424778 46-47 21.763061828881316 26.769679307779708 28.628502215226074 22.8387566481129 48-49 21.861440465240534 26.827060907496623 28.667036453818618 22.64446217344423 50-51 21.722345258816496 27.035631980335072 28.43561838953273 22.806404371315704 52-53 21.51107205591927 27.25133444666315 28.295069109014552 22.942524388403026 54-55 21.404008073077186 27.07173845113348 28.41974115642764 23.104512319361685 56-57 21.715645551751535 26.970890885616623 28.140733119110067 23.172730443521775 58-59 21.38746537420207 27.06309186765739 28.403997311639966 23.14544544650057 60-61 21.68023380984727 27.237144759994575 27.997975896518618 23.084645533639538 62-63 21.59717680116827 27.058184336671598 27.930921298142863 23.41371756401727 64-65 21.197018437175835 26.93584995115723 28.012338719193004 23.854792892473935 66-67 21.33330321604953 26.906476252637695 27.801944459625005 23.95827607168777 68-69 21.213342699170447 27.095667615134328 27.739884751382952 23.95110493431227 70-71 21.31345417656169 26.884517276733376 27.714264884396943 24.087763662307985 72-73 21.331950066885888 27.197360553773304 27.4265918469599 24.0440975323809 74-75 21.430685344334403 27.31055006656962 27.229268667561968 24.029495921534018 76-77 21.41411800719697 27.18830074859625 27.17738862570402 24.220192618502765 78-79 21.230735940372057 27.29492300369079 27.192732844595618 24.281608211341535 80-81 21.135915770568666 27.510103439877792 27.05064410766787 24.303336681885675 82-83 21.440726126233063 27.232190137834966 27.158070067495288 24.16901366843668 84-85 21.627365541280703 27.309669423058036 26.721232456742534 24.34173257891873 86-87 21.148186406686868 27.458456764106277 26.94093340374744 24.45242342545941 88-89 21.30641777257 27.10356111794204 26.930148751439525 24.65987235804844 90-91 21.46523028392349 27.016728094801902 26.90100610481398 24.617035516460625 92-93 21.45234474247001 27.117206030733783 26.77317339627951 24.6572758305167 94-95 21.052262390732327 26.90945482457835 27.03538545978433 25.002897324905 96-97 21.092351548823135 26.897222733479147 27.02210556052545 24.988320157172268 98-99 21.017991283537196 27.035424423198705 26.8546778503787 25.0919064428854 100 21.071720057458755 26.93998745256988 26.87182421268729 25.116468277284078 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1855.0 1 1304.0 2 1708.5 3 2709.5 4 2043.5 5 1425.5 6 1912.5 7 3450.5 8 5445.0 9 6438.5 10 5842.0 11 5211.0 12 5593.5 13 6279.0 14 7218.5 15 8230.0 16 9168.0 17 10289.5 18 11576.0 19 12831.5 20 14302.0 21 16370.0 22 19277.0 23 23338.0 24 28649.0 25 35599.5 26 44561.5 27 55244.5 28 68527.5 29 85243.0 30 104342.5 31 122132.0 32 144197.0 33 166159.5 34 184896.0 35 204670.0 36 226472.0 37 253335.5 38 270430.5 39 274975.5 40 279109.0 41 285791.5 42 295989.5 43 310367.0 44 326851.5 45 348015.0 46 366787.0 47 380286.0 48 392556.5 49 397234.0 50 394049.5 51 387250.0 52 372524.0 53 352879.0 54 330695.5 55 298458.5 56 264242.5 57 227377.0 58 191718.0 59 164688.5 60 134456.5 61 103525.5 62 79713.0 63 60434.0 64 42979.0 65 29187.0 66 20543.0 67 15304.0 68 11524.0 69 8354.5 70 6104.0 71 4346.5 72 3150.0 73 2268.5 74 1564.5 75 1052.5 76 668.5 77 422.5 78 255.5 79 144.5 80 100.0 81 74.5 82 43.0 83 32.5 84 22.5 85 14.0 86 14.0 87 9.0 88 3.5 89 3.0 90 2.5 91 3.0 92 3.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05191792431025775 2 1.0700314161223773E-5 3 1.0700314161223773E-5 4 0.01766621868018045 5 0.004344327549456852 6 0.0 7 1.8190534074080414E-4 8 4.387128806101747E-4 9 0.002129362518083531 10-11 7.597223054468879E-4 12-13 2.9960879651426566E-4 14-15 3.210094248367132E-5 16-17 1.0165298453162584E-4 18-19 0.0 20-21 1.605047124183566E-5 22-23 0.0 24-25 1.0165298453162584E-4 26-27 2.6750785403059436E-5 28-29 9.095267037040207E-4 30-31 8.239241904142305E-4 32-33 0.014145815321137827 34-35 0.022470659738569924 36-37 0.03008928342136125 38-39 0.0201647420368262 40-41 1.7655518366019226E-4 42-43 0.0019581574915039507 44-45 0.009277172377781012 46-47 0.014199316891943948 48-49 0.005398308494337394 50-51 0.010325803165580941 52-53 0.01582041448736935 54-55 0.00941627646187692 56-57 0.0030549396930293874 58-59 0.008447898030286168 60-61 0.0015836464958611186 62-63 9.89779059913199E-4 64-65 0.0188593037091569 66-67 0.04559938879805511 68-69 0.05554533081091261 70-71 0.0486543284910845 72-73 0.04134066376188805 74-75 0.028559138496306254 76-77 0.02889084823530419 78-79 0.012583569453599158 80-81 0.0020116590623100693 82-83 0.027515857865586933 84-85 0.04817816451091004 86-87 0.0491037416858559 88-89 0.06039257312594698 90-91 0.035337787517441516 92-93 0.026868488858832893 94-95 0.007415317713728075 96-97 0.0038521130980405586 98-99 0.02194634434466996 100 0.018982357322010975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9345520.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.70411523420674 #Duplication Level Percentage of deduplicated Percentage of total 1 75.98350127797875 46.88494718754895 2 14.6888258495045 18.12722005746041 3 4.510706207100785 8.349874067717954 4 1.8294792117893306 4.515455844113383 5 0.8872789558098078 2.7374381467087496 6 0.5222675912144269 1.9335635778831943 7 0.339989267918416 1.468511587622207 8 0.2232591324970409 1.1020805790949153 9 0.16451719106748422 0.9136248944072448 >10 0.791918061772239 8.60477911481644 >50 0.040408760956277 1.710871317578922 >100 0.01645121469837282 1.7587648212409848 >500 7.985228188102718E-4 0.31288172926855135 >1k 5.100504518780774E-4 0.6111492898211327 >5k 2.217610549545824E-5 0.09618181134662958 >10k+ 6.652831648637473E-5 0.8726559733703164 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27191 0.2909522423578356 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22343 0.2390771193042228 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14240 0.15237247365582654 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.3501570806118865E-5 2 0.0 0.0 0.0 0.0 5.3501570806118865E-5 3 0.0 0.0 0.0 1.0700314161223773E-5 5.3501570806118865E-5 4 0.0 1.0700314161223773E-5 0.0 2.1400628322447547E-5 5.3501570806118865E-5 5 0.0 1.0700314161223773E-5 0.0 2.1400628322447547E-5 5.3501570806118865E-5 6 1.0700314161223773E-5 1.0700314161223773E-5 0.0 2.1400628322447547E-5 7.490219912856641E-5 7 1.0700314161223773E-5 1.0700314161223773E-5 0.0 2.1400628322447547E-5 7.490219912856641E-5 8 1.0700314161223773E-5 1.0700314161223773E-5 0.0 2.1400628322447547E-5 7.490219912856641E-5 9 1.0700314161223773E-5 1.0700314161223773E-5 0.0 5.3501570806118865E-5 7.490219912856641E-5 10-11 1.0700314161223773E-5 1.0700314161223773E-5 0.0 7.490219912856641E-5 7.490219912856641E-5 12-13 1.0700314161223773E-5 1.0700314161223773E-5 0.0 1.1770345577346151E-4 9.630282745101396E-5 14-15 1.605047124183566E-5 1.0700314161223773E-5 0.0 1.5515455533774471E-4 1.2840376993468529E-4 16-17 6.420188496734264E-5 1.0700314161223773E-5 0.0 2.0330596906325171E-4 1.8190534074080414E-4 18-19 6.420188496734264E-5 1.0700314161223773E-5 0.0 2.889084823530419E-4 1.8725549782141603E-4 20-21 6.420188496734264E-5 1.0700314161223773E-5 0.0 5.778169647060838E-4 2.3540691154692303E-4 22-23 6.420188496734264E-5 1.0700314161223773E-5 0.0 0.0017227505799570276 2.4075706862753491E-4 24-25 7.490219912856641E-5 2.6750785403059432E-5 0.0 0.004478081476472149 2.5145738278875866E-4 26-27 7.490219912856641E-5 4.280125664489509E-5 0.0 0.008806358554687164 2.6750785403059434E-4 28-29 7.490219912856641E-5 4.280125664489509E-5 0.0 0.022866571362535205 2.6750785403059434E-4 30-31 7.490219912856641E-5 4.815141372550698E-5 0.0 0.07037061608128814 2.6750785403059434E-4 32-33 7.490219912856641E-5 5.3501570806118865E-5 0.0 0.1750785403059434 2.9960879651426566E-4 34-35 7.490219912856641E-5 5.3501570806118865E-5 0.0 0.3265468374151465 2.9960879651426566E-4 36-37 7.490219912856641E-5 5.885172788673076E-5 0.0 0.5645913764028112 2.9960879651426566E-4 38-39 7.490219912856641E-5 6.955204204795453E-5 0.0 0.9250100582953116 2.9960879651426566E-4 40-41 8.560251328979019E-5 8.02523562091783E-5 0.0 1.318401758275623 2.9960879651426566E-4 42-43 8.560251328979019E-5 1.0700314161223773E-4 0.0 1.6957055359145343 3.1030911067548943E-4 44-45 8.560251328979019E-5 1.0700314161223773E-4 0.0 2.1324549088761247 3.263595819173251E-4 46-47 8.560251328979019E-5 1.1770345577346151E-4 0.0 2.6394571944632297 3.31709738997937E-4 48-49 8.560251328979019E-5 1.1770345577346151E-4 0.0 3.1758050916374905 3.31709738997937E-4 50-51 9.630282745101396E-5 1.1770345577346151E-4 0.0 3.7214141107182908 3.531103673203845E-4 52-53 9.630282745101396E-5 1.1770345577346151E-4 0.0 4.250089882638955 3.8521130980405583E-4 54-55 9.630282745101396E-5 1.230536128540734E-4 0.0 4.813530975269433 3.8521130980405583E-4 56-57 9.630282745101396E-5 1.2840376993468529E-4 0.0 5.435513486675969 4.0126178104589146E-4 58-59 9.630282745101396E-5 1.2840376993468529E-4 0.0 6.120028634040695 4.066119381265034E-4 60-61 9.630282745101396E-5 1.2840376993468529E-4 0.0 6.804158570095618 4.066119381265034E-4 62-63 9.630282745101396E-5 1.2840376993468529E-4 0.0 7.495912479990412 4.601135089326223E-4 64-65 9.630282745101396E-5 1.2840376993468529E-4 0.0 8.158946746676483 4.7081382309384606E-4 66-67 9.630282745101396E-5 1.2840376993468529E-4 0.0 8.873321120708104 4.8686429433568174E-4 68-69 9.630282745101396E-5 1.2840376993468529E-4 0.0 9.642855614240833 4.922144514162936E-4 70-71 9.630282745101396E-5 1.3375392701529717E-4 0.0 10.433908439551786 4.922144514162936E-4 72-73 1.1770345577346151E-4 1.3910408409590906E-4 0.0 11.198012523647694 5.18965236819353E-4 74-75 1.1770345577346151E-4 1.3910408409590906E-4 0.0 11.928742327874746 5.403658651418005E-4 76-77 1.1770345577346151E-4 1.3910408409590906E-4 0.0 12.697581300986997 5.564163363836362E-4 78-79 1.1770345577346151E-4 1.3910408409590906E-4 0.0 13.51259747986201 5.564163363836362E-4 80-81 1.1770345577346151E-4 1.3910408409590906E-4 0.0 14.346970527054674 5.831671217866956E-4 82-83 1.1770345577346151E-4 1.3910408409590906E-4 0.0 15.1771597514103 6.527191638346502E-4 84-85 1.230536128540734E-4 1.3910408409590906E-4 0.0 15.972936765423432 6.580693209152621E-4 86-87 1.2840376993468529E-4 1.3910408409590906E-4 0.0 16.77259799347709 7.115708917213809E-4 88 1.2840376993468529E-4 1.3910408409590906E-4 0.0 17.390054271993424 7.597223054468879E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14205 0.0 53.055912 1 TACCTGG 9495 0.0 47.61207 2 GTATCAA 25830 0.0 40.95431 1 ACCTGGG 11060 0.0 39.856018 3 ATCAACG 31750 0.0 32.740677 3 CAACGCA 31920 0.0 32.650097 5 GTACCTG 14410 0.0 32.007114 1 TATCAAC 33205 0.0 31.63086 2 TCAACGC 33240 0.0 31.401138 4 AACGCAG 34235 0.0 30.9076 6 CCTGGGG 10555 0.0 29.074535 4 ACGCAGA 38160 0.0 27.211561 7 TAACGCA 985 0.0 27.195465 4 CGCAGAG 38950 0.0 26.50268 8 ATAACGC 965 0.0 26.297392 3 GTACATG 28395 0.0 25.680971 1 TACATGG 29190 0.0 24.245314 2 CTGGGGG 7425 0.0 24.047667 5 GCAGAGT 42785 0.0 23.632824 9 TGGGGGG 14690 0.0 23.508926 6 >>END_MODULE