FastQCFastQC Report
Mon 6 Jun 2016
ERR1211437_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211437_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8227445
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256450.31170065554980925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196330.238628152482332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125770.152866412355233No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125000.053.035551
TACCTGG78350.044.1522142
GTATCAA227050.040.5910571
ACCTGGG95900.035.1490143
ATCAACG277350.032.460353
CAACGCA279450.032.1657525
TATCAAC285650.031.931822
TCAACGC293500.030.801974
AACGCAG300450.030.4027736
GTACCTG121650.029.0490671
CCTGGGG91750.027.2591864
GTACATG275550.027.0855581
ACGCAGA333550.027.0479627
CGCAGAG339900.026.4577338
TACATGG276850.026.401652
ACATGGG274200.025.666523
CATGGGG207900.024.760934
GTACTAG30250.024.2986551
GCAGAGT374350.023.293599
ATGGGGG156100.023.1597255
Produced by FastQC (version 0.11.5)