FastQCFastQC Report
Mon 6 Jun 2016
ERR1211437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211437_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8227445
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229750.2792482964030753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187710.22815102379900443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121910.14817479788682877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124800.050.4099771
TACCTGG81400.042.086532
GTATCAA224600.040.696921
ACCTGGG100200.032.9708563
ATCAACG277650.032.2433323
CAACGCA280900.031.9002485
TATCAAC289350.031.4590852
TCAACGC293400.030.5767354
AACGCAG300900.030.0437586
GTACCTG127900.027.645361
ACGCAGA333950.026.6623947
CCTGGGG88500.026.4442564
CGCAGAG339100.026.0771758
GTACATG272400.025.7880481
TACATGG275950.025.0338082
ACATGGG270450.024.5526163
TGGGGGG152650.023.6701436
CATGGGG203250.023.5376174
ATGGGGG148000.023.3674435
GCAGAGT374500.022.9974339
Produced by FastQC (version 0.11.5)