FastQCFastQC Report
Mon 6 Jun 2016
ERR1211436_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211436_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6254925
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179820.2874854614563724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140090.22396751359928377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86530.1383389888767651No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG54750.040.4121442
ACCTGGG66450.032.7553863
GTATCAA224950.032.4917371
GGTATCA147050.031.4377271
GTACATG203150.029.5760731
TACATGG209800.028.0256142
ATCAACG260700.027.3552863
CAACGCA260400.027.2421595
TATCAAC269250.026.8380412
TCAACGC268650.026.5982744
ACATGGG210550.026.4135173
AACGCAG277400.025.8440236
GTACCTG88800.025.7380681
GTACTAG24000.025.7221471
CATGGGG165500.025.138654
ATGGGGG129700.024.683145
ACGCAGA299650.023.5957497
CGCAGAG302450.023.1423028
CCTGGGG65750.023.094234
TAACGCA7200.022.8524234
Produced by FastQC (version 0.11.5)