FastQCFastQC Report
Mon 6 Jun 2016
ERR1211436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211436_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6254925
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157310.2514978197180622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127190.20334376511309088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84850.13565310535298186No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG55150.041.836972
ACCTGGG67750.033.2943153
GGTATCA150400.032.9175151
GTATCAA229850.032.094081
GTACATG210800.027.2773631
CAACGCA271300.026.5504255
ATCAACG270500.026.5457023
TATCAAC281350.026.1558652
TACATGG215450.026.1078972
TCAACGC278650.025.8032254
TGGGGGG129200.025.783926
GTACCTG92000.025.7055381
AACGCAG287050.025.4856836
ACATGGG211100.025.4001063
ATGGGGG124550.024.483875
CATGGGG166350.023.7300174
CCTGGGG67150.023.5844354
ACGCAGA311100.023.1227997
CGCAGAG316500.022.6689038
CTGGGGG47450.022.3795495
Produced by FastQC (version 0.11.5)