##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211436_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6254925 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10027442375408 33.0 31.0 34.0 30.0 34.0 2 32.23936945686799 33.0 31.0 34.0 30.0 34.0 3 32.319477371831 34.0 31.0 34.0 30.0 34.0 4 35.95277097647054 37.0 35.0 37.0 35.0 37.0 5 35.813367386499436 37.0 35.0 37.0 35.0 37.0 6 35.849367338537235 37.0 35.0 37.0 35.0 37.0 7 35.783020419909114 37.0 35.0 37.0 33.0 37.0 8 35.7638465689037 37.0 35.0 37.0 33.0 37.0 9 37.36008713134051 39.0 37.0 39.0 34.0 39.0 10-11 37.337765840517676 39.0 37.0 39.0 34.0 39.0 12-13 37.251674080824316 39.0 37.0 39.0 33.5 39.0 14-15 38.46718345943397 40.0 38.0 41.0 34.0 41.0 16-17 38.45544654812008 40.0 38.0 41.0 34.0 41.0 18-19 38.46102439277849 40.0 38.0 41.0 34.0 41.0 20-21 38.41809094433586 40.0 38.0 41.0 34.0 41.0 22-23 38.3186321178911 40.0 38.0 41.0 34.0 41.0 24-25 38.199208064045536 40.0 37.5 41.0 33.5 41.0 26-27 38.08092175365812 40.0 37.0 41.0 33.0 41.0 28-29 38.006636210665995 40.0 37.0 41.0 33.0 41.0 30-31 38.06696011542904 40.0 37.0 41.0 33.0 41.0 32-33 37.91513791132587 40.0 37.0 41.0 33.0 41.0 34-35 37.811821964292136 40.0 37.0 41.0 33.0 41.0 36-37 37.67754889147352 40.0 37.0 41.0 33.0 41.0 38-39 37.56623220901929 40.0 37.0 41.0 32.0 41.0 40-41 37.510044724756895 40.0 37.0 41.0 32.0 41.0 42-43 37.38437455285235 40.0 36.0 41.0 32.0 41.0 44-45 37.241074513283536 40.0 36.0 41.0 31.0 41.0 46-47 37.038311010923394 39.5 36.0 41.0 31.0 41.0 48-49 36.830559599035965 39.0 36.0 41.0 30.5 41.0 50-51 36.6581716807156 39.0 35.0 41.0 30.0 41.0 52-53 36.45845233955643 39.0 35.0 40.5 30.0 41.0 54-55 36.2672127963165 39.0 35.0 40.0 29.5 41.0 56-57 36.04212808307054 38.0 35.0 40.0 29.0 41.0 58-59 35.78604699496796 38.0 34.5 40.0 28.0 41.0 60-61 35.52681311126832 38.0 34.0 40.0 28.0 41.0 62-63 35.23185361934796 37.0 34.0 40.0 28.0 41.0 64-65 34.886689528651424 37.0 34.0 39.5 27.0 41.0 66-67 34.53300567153083 36.0 33.5 39.0 26.0 41.0 68-69 34.16757347210398 36.0 33.0 39.0 26.0 40.0 70-71 33.793601362126644 35.0 33.0 38.5 26.0 40.0 72-73 33.436584771200295 35.0 32.5 37.5 26.0 40.0 74-75 33.06921258048658 35.0 32.0 37.0 25.0 39.0 76-77 32.04834358525482 34.0 30.5 36.0 24.5 38.5 78-79 32.53126640207516 35.0 32.0 36.0 25.0 38.5 80-81 32.42419597357282 35.0 32.0 36.0 25.0 37.5 82-83 32.15646774341819 35.0 32.0 35.0 25.0 37.0 84-85 31.882185797591497 34.0 31.0 35.0 24.0 36.5 86-87 31.63144378229955 34.0 31.0 35.0 24.0 36.0 88-89 31.406362666219017 34.0 31.0 35.0 23.5 36.0 90-91 31.189634647897456 34.0 31.0 35.0 23.0 35.5 92-93 31.01316914271554 34.0 31.0 35.0 22.5 35.0 94-95 30.846212224766884 34.0 30.0 35.0 20.0 35.0 96-97 30.679849958232914 34.0 30.0 35.0 20.0 35.0 98-99 30.491451216441444 34.0 30.0 35.0 20.0 35.0 100 28.621343501320958 32.0 26.0 34.0 16.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2248426874356788 1101 2 -0.2507116911960878 1101 3 -0.25473744980556745 1101 4 -0.13598142389408707 1101 5 -0.17146354607032066 1101 6 -0.25838651511030974 1101 7 -0.22101172252394718 1101 8 -0.24227147251622938 1101 9 -0.35843173346074053 1101 10-11 -0.3623363237776829 1101 12-13 -0.37840422376908833 1101 14-15 -0.6136887467479966 1101 16-17 -0.5699882819051112 1101 18-19 -0.5370122340241252 1101 20-21 -0.5779612966040091 1101 22-23 -0.6375141557187902 1101 24-25 -0.7133967187683936 1101 26-27 -0.6555569589560548 1101 28-29 -0.6433782243736346 1101 30-31 -0.60545602644941 1101 32-33 -0.5658274779967343 1101 34-35 -0.6460367510544884 1101 36-37 -0.6558849113668188 1101 38-39 -0.6691508483261401 1101 40-41 -0.6735137753788578 1101 42-43 -0.5469392757971789 1101 44-45 -0.6937716434322923 1101 46-47 -0.7244954235374763 1101 48-49 -0.6563780566417137 1101 50-51 -0.7426956412483676 1101 52-53 -0.7159484315708511 1101 54-55 -0.707149300238541 1101 56-57 -0.7945000651449519 1101 58-59 -0.8183746088110482 1101 60-61 -0.8130527065978796 1101 62-63 -0.9005886319416945 1101 64-65 -0.8862965345215557 1101 66-67 -0.9117494596872433 1101 68-69 -0.9482125485436015 1101 70-71 -0.8821178424386886 1101 72-73 -0.9247367155543174 1101 74-75 -1.0340234589547634 1101 76-77 -0.8297440396239999 1101 78-79 -0.811833342080682 1101 80-81 -0.6539085665564919 1101 82-83 -0.7945957673491755 1101 84-85 -0.88112785405308 1101 86-87 -0.9703025120027675 1101 88-89 -0.9229488053596491 1101 90-91 -0.9645660707330883 1101 92-93 -1.0943336268992923 1101 94-95 -1.1232268105159662 1101 96-97 -1.1413541925375874 1101 98-99 -1.2440268491418855 1101 100 -1.1595746727299705 1102 1 -0.271107409589721 1102 2 -0.2140487549859209 1102 3 -0.2984797307800413 1102 4 -0.1506344232590351 1102 5 -0.15970579003244012 1102 6 -0.23256820100392162 1102 7 -0.2970044330366264 1102 8 -0.2800601202403641 1102 9 -0.47187803241932613 1102 10-11 -0.4315070715442815 1102 12-13 -0.39370932251417656 1102 14-15 -0.5906220095630488 1102 16-17 -0.6256933479234732 1102 18-19 -0.6565602156815729 1102 20-21 -0.6901948319469184 1102 22-23 -0.7232982187800587 1102 24-25 -0.7610233497473544 1102 26-27 -0.6966778150325084 1102 28-29 -0.6765364307935258 1102 30-31 -0.6831147802626631 1102 32-33 -0.6250823217461274 1102 34-35 -0.719824669245483 1102 36-37 -0.5969987302244206 1102 38-39 -0.6386200326126428 1102 40-41 -0.672259372855784 1102 42-43 -0.732153275430953 1102 44-45 -0.6681792995368028 1102 46-47 -0.6736426294937345 1102 48-49 -0.6717420829633554 1102 50-51 -0.8064807250716299 1102 52-53 -0.7457162325690163 1102 54-55 -0.7493320702047654 1102 56-57 -0.8140438098609479 1102 58-59 -0.7884815258371205 1102 60-61 -0.8970234074172154 1102 62-63 -0.8907731726572408 1102 64-65 -0.8037858739872448 1102 66-67 -0.854734837856185 1102 68-69 -0.8566930303666354 1102 70-71 -0.801157561573298 1102 72-73 -0.9884385215627844 1102 74-75 -1.020087695857253 1102 76-77 -0.8074283855376407 1102 78-79 -0.7860570321352647 1102 80-81 -0.6665554715438091 1102 82-83 -0.7378341444934549 1102 84-85 -0.7489395419726357 1102 86-87 -0.845403321989096 1102 88-89 -0.8133886826734056 1102 90-91 -0.7995341573108483 1102 92-93 -0.9474527463167561 1102 94-95 -1.083725854038608 1102 96-97 -1.2803504659771257 1102 98-99 -1.1870358470419227 1102 100 -1.2104944299288718 1103 1 -0.38735266309901206 1103 2 -0.3299895716935666 1103 3 -0.37291696038569455 1103 4 -0.22469815851378883 1103 5 -0.15143493270728214 1103 6 -0.3142929473390126 1103 7 -0.35394286871778746 1103 8 -0.4186834239535173 1103 9 -0.5100415684770923 1103 10-11 -0.49047297851306837 1103 12-13 -0.5652961774583076 1103 14-15 -0.7733105950310062 1103 16-17 -0.8328881232821885 1103 18-19 -0.7260525357039853 1103 20-21 -0.8038416735853176 1103 22-23 -0.8865164659243874 1103 24-25 -0.8726726288254412 1103 26-27 -0.9225387973226518 1103 28-29 -0.9172948772126475 1103 30-31 -0.7849768538566124 1103 32-33 -0.8006694081789405 1103 34-35 -0.7980046234924174 1103 36-37 -0.7641965402247308 1103 38-39 -0.8094910233842185 1103 40-41 -0.8527424936345156 1103 42-43 -0.9400109059983563 1103 44-45 -0.8597043333847267 1103 46-47 -0.8715647267423279 1103 48-49 -0.8401153302632949 1103 50-51 -0.8425012886590437 1103 52-53 -0.9427094571480623 1103 54-55 -0.9513820581440058 1103 56-57 -0.939799552336595 1103 58-59 -1.113315836350985 1103 60-61 -1.049949648118762 1103 62-63 -1.0820047119208382 1103 64-65 -1.095684596714385 1103 66-67 -1.067667225652258 1103 68-69 -1.0689732620109496 1103 70-71 -0.9727855106224439 1103 72-73 -1.0198344177936036 1103 74-75 -1.0443577770144614 1103 76-77 -0.9667397747810043 1103 78-79 -0.8774075254590556 1103 80-81 -0.8334053190336164 1103 82-83 -0.897142129491435 1103 84-85 -0.8876552466319758 1103 86-87 -0.8514405080204064 1103 88-89 -0.9521907092693134 1103 90-91 -1.035138333055503 1103 92-93 -1.0277975574686735 1103 94-95 -1.103816737663994 1103 96-97 -1.0697144848541527 1103 98-99 -1.139756228161783 1103 100 -1.2973605594380402 1104 1 -0.3699464053887347 1104 2 -0.31751067092949015 1104 3 -0.3375085506512612 1104 4 -0.1979482681710678 1104 5 -0.23642195741150118 1104 6 -0.313069154939825 1104 7 -0.2882997679231636 1104 8 -0.34422606839761016 1104 9 -0.47803963283025297 1104 10-11 -0.4538385734429795 1104 12-13 -0.49514412466420055 1104 14-15 -0.7229232882040577 1104 16-17 -0.7147223971062076 1104 18-19 -0.6897370056872631 1104 20-21 -0.7286612653273394 1104 22-23 -0.7469786456663812 1104 24-25 -0.7850632224335996 1104 26-27 -0.8205192186024348 1104 28-29 -0.8074480816529856 1104 30-31 -0.788458197187687 1104 32-33 -0.6897378499881341 1104 34-35 -0.821935367086283 1104 36-37 -0.756968736278246 1104 38-39 -0.9553760543330228 1104 40-41 -1.032400559955633 1104 42-43 -0.8677130707016332 1104 44-45 -0.9142018913072718 1104 46-47 -0.8447755201029779 1104 48-49 -0.8354555995012944 1104 50-51 -0.8288896196066773 1104 52-53 -0.7780027206173017 1104 54-55 -0.7430174043575022 1104 56-57 -0.8024360609662295 1104 58-59 -0.8929925419623075 1104 60-61 -0.8660164776232264 1104 62-63 -0.9723100413626398 1104 64-65 -0.9405558607789075 1104 66-67 -1.0213995935935571 1104 68-69 -0.9519301155421047 1104 70-71 -0.9943989486327922 1104 72-73 -0.9899777751079455 1104 74-75 -1.031239688115697 1104 76-77 -0.917261148649672 1104 78-79 -0.8780264282204584 1104 80-81 -0.8219696310712443 1104 82-83 -0.8239004889010104 1104 84-85 -0.8476449638141013 1104 86-87 -0.876260626019473 1104 88-89 -1.039650927908312 1104 90-91 -0.8754448679930995 1104 92-93 -1.0551546321746557 1104 94-95 -0.9706259751150874 1104 96-97 -1.1297650336790603 1104 98-99 -1.1889058824476209 1104 100 -1.1551615613335429 1105 1 -0.31572100836312345 1105 2 -0.3145887203751485 1105 3 -0.3807221217417833 1105 4 -0.20218200813833676 1105 5 -0.2571778249701495 1105 6 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68-69 -0.9345251226742377 1105 70-71 -0.9254999633626539 1105 72-73 -0.9310935707051016 1105 74-75 -0.8837592062548154 1105 76-77 -0.86062819381144 1105 78-79 -0.7217706966963817 1105 80-81 -0.6516070136679097 1105 82-83 -0.7232830757543418 1105 84-85 -0.6965557385503587 1105 86-87 -0.8083676996223055 1105 88-89 -0.6932543398670497 1105 90-91 -0.697411960452218 1105 92-93 -0.8266700827529085 1105 94-95 -0.802481241420427 1105 96-97 -0.8570360441644489 1105 98-99 -0.9071729290456823 1105 100 -1.0973213624458538 1106 1 -0.40219561509083945 1106 2 -0.3519933170119174 1106 3 -0.3154284517033652 1106 4 -0.18623416464249232 1106 5 -0.24182797833206848 1106 6 -0.3251724587452074 1106 7 -0.34734600116586023 1106 8 -0.3793735414203212 1106 9 -0.44201935192959496 1106 10-11 -0.4643568310837125 1106 12-13 -0.46879836932075847 1106 14-15 -0.6518723715035932 1106 16-17 -0.7064543686005322 1106 18-19 -0.7070812214377256 1106 20-21 -0.6806451720531399 1106 22-23 -0.705695794320711 1106 24-25 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0.9518153465879209 2311 34-35 0.91365938279273 2311 36-37 1.0286770732072839 2311 38-39 1.0440496157620487 2311 40-41 1.0180805579997454 2311 42-43 1.1249751153075707 2311 44-45 1.0530382380714514 2311 46-47 1.1091224038179703 2311 48-49 1.0716564877229615 2311 50-51 1.065064645415454 2311 52-53 1.1094743874253865 2311 54-55 1.0322430533114257 2311 56-57 1.089737307457149 2311 58-59 1.1502444127168374 2311 60-61 1.1575568950168318 2311 62-63 1.0916091487707789 2311 64-65 1.1538482452277847 2311 66-67 1.1601924051886243 2311 68-69 1.1557587967814058 2311 70-71 1.158368115498348 2311 72-73 1.182708169094866 2311 74-75 1.1265325375860442 2311 76-77 0.9784184997329568 2311 78-79 0.9225584143830687 2311 80-81 0.9400751458680503 2311 82-83 0.9841535438552853 2311 84-85 1.0134052557436668 2311 86-87 1.082724012674447 2311 88-89 1.1064965210552948 2311 90-91 1.1556302145320707 2311 92-93 1.2573583515400415 2311 94-95 1.2638233064817115 2311 96-97 1.282321895603264 2311 98-99 1.296530427426891 2311 100 1.3926731032214263 2312 1 0.11850284062759897 2312 2 0.12956302755326732 2312 3 0.3379194720486467 2312 4 0.25868956766228024 2312 5 0.3232563697301245 2312 6 0.4469426473193394 2312 7 0.4673584307903056 2312 8 0.3223075672539295 2312 9 0.4420469994345453 2312 10-11 0.31088493805301454 2312 12-13 0.4763462904960747 2312 14-15 0.8300708088060134 2312 16-17 0.8574944947591518 2312 18-19 0.8972564296101737 2312 20-21 0.8728464635019435 2312 22-23 0.5085960757472066 2312 24-25 0.43080729816852426 2312 26-27 0.7234370773580778 2312 28-29 0.8962214206501002 2312 30-31 0.8510416011073119 2312 32-33 0.7217657894074847 2312 34-35 0.9036763104434868 2312 36-37 0.9881845001390559 2312 38-39 0.9958040833358268 2312 40-41 1.0307647332550474 2312 42-43 1.001055915315824 2312 44-45 0.8917360446082085 2312 46-47 1.102532940397687 2312 48-49 1.1885172564474615 2312 50-51 1.2083767101392482 2312 52-53 1.2410317255988232 2312 54-55 1.248834543356324 2312 56-57 1.1991567336166966 2312 58-59 1.2176680434864195 2312 60-61 1.1934410520602299 2312 62-63 1.1897928457927875 2312 64-65 1.2245670681313996 2312 66-67 1.2204149682152519 2312 68-69 1.236866045437047 2312 70-71 1.080190928585452 2312 72-73 1.1813469566926074 2312 74-75 1.2292384421912175 2312 76-77 1.238340359436915 2312 78-79 1.1432084319190494 2312 80-81 0.9326443977994074 2312 82-83 0.9775661404759504 2312 84-85 0.924803692041575 2312 86-87 1.0970298578593258 2312 88-89 0.7568583781649245 2312 90-91 1.152684811654897 2312 92-93 1.2967198482089657 2312 94-95 1.3304781242738457 2312 96-97 1.3716859687473644 2312 98-99 1.370936799572128 2312 100 1.5197192080390458 2313 1 -0.39368540165694554 2313 2 -0.03146826577879125 2313 3 0.22723718066441023 2313 4 0.06287648285431402 2313 5 0.22203285814377693 2313 6 0.3185712642597025 2313 7 0.11065474511398321 2313 8 0.024143422794651315 2313 9 0.24174938269243285 2313 10-11 0.35654055704390686 2313 12-13 0.491173072359139 2313 14-15 0.5574892380196701 2313 16-17 0.7227879699856601 2313 18-19 0.6130828021991093 2313 20-21 0.7857894331680342 2313 22-23 0.8455086927805269 2313 24-25 0.6965552157909585 2313 26-27 0.8728709793715765 2313 28-29 0.7439109710195098 2313 30-31 0.7725833082638545 2313 32-33 0.880413200823952 2313 34-35 0.8980555783778783 2313 36-37 0.7494271656971065 2313 38-39 0.5470748038246924 2313 40-41 0.7299863108669271 2313 42-43 0.7328760587037877 2313 44-45 0.881623760330676 2313 46-47 0.9171135247946651 2313 48-49 0.9641794560297754 2313 50-51 0.9292394820167615 2313 52-53 0.8303323847123565 2313 54-55 0.9572376580715698 2313 56-57 0.991979414991981 2313 58-59 0.8998628921840961 2313 60-61 0.9516629808473951 2313 62-63 1.0330361126380936 2313 64-65 1.0901998298867994 2313 66-67 1.067267106138189 2313 68-69 1.0688491901714698 2313 70-71 0.9796276727803743 2313 72-73 1.0738360825243944 2313 74-75 0.6804001538149222 2313 76-77 0.8245231365562411 2313 78-79 0.7679539625394369 2313 80-81 0.9222207556843074 2313 82-83 0.9412806557063078 2313 84-85 0.946932046963294 2313 86-87 0.9737131913647943 2313 88-89 0.9989539757352794 2313 90-91 1.1181718349538237 2313 92-93 0.8111874303413735 2313 94-95 0.9483829007596754 2313 96-97 1.2070505921133154 2313 98-99 1.2581201733881677 2313 100 1.4356423349392706 2314 1 -0.2906588215187611 2314 2 -0.1428203062796598 2314 3 0.12008058851478864 2314 4 -0.12457003453093307 2314 5 0.17393014102084692 2314 6 0.22529521400129227 2314 7 0.13904128695077844 2314 8 0.20021370369070013 2314 9 0.26018419078567945 2314 10-11 0.3817626077920053 2314 12-13 0.5276863696416854 2314 14-15 0.6023628521089037 2314 16-17 0.7660842591372017 2314 18-19 0.8183509067340182 2314 20-21 0.5001536011351817 2314 22-23 0.7048946546555683 2314 24-25 0.8681954307189343 2314 26-27 0.8429992387195924 2314 28-29 0.787559920822936 2314 30-31 0.7391291341055037 2314 32-33 0.8686355871007478 2314 34-35 0.9457499881977469 2314 36-37 0.8325975780016961 2314 38-39 0.8689440253321692 2314 40-41 0.8815681751286704 2314 42-43 0.7241023594133438 2314 44-45 0.7620924865005065 2314 46-47 0.8928724523724014 2314 48-49 0.7976536320677567 2314 50-51 0.842655482572404 2314 52-53 0.9430683780380491 2314 54-55 1.0209893728128208 2314 56-57 0.946138760002043 2314 58-59 0.9263767438721686 2314 60-61 0.9602641755191641 2314 62-63 0.7404018382992703 2314 64-65 0.8800962632249991 2314 66-67 0.9563059575995538 2314 68-69 0.9622652432822818 2314 70-71 0.9581964728572103 2314 72-73 1.002357108920691 2314 74-75 1.0325963622582108 2314 76-77 0.8231170796449696 2314 78-79 0.7585973388154983 2314 80-81 0.8400682391366416 2314 82-83 0.8591593631129228 2314 84-85 0.7369670043956695 2314 86-87 0.881499966381682 2314 88-89 0.9319614360963122 2314 90-91 1.0182394147202096 2314 92-93 1.0843763173341543 2314 94-95 1.0905466723577888 2314 96-97 1.1793713810426816 2314 98-99 1.2145901425393113 2314 100 1.2528983717765314 2315 1 -0.19163707711377498 2315 2 0.04794652399787935 2315 3 0.06595882344035431 2315 4 -0.094404173570382 2315 5 0.20319204748485475 2315 6 0.33693498176951664 2315 7 0.40189178523917946 2315 8 0.3365617344618528 2315 9 0.5913699688180074 2315 10-11 0.5322267113014334 2315 12-13 0.5952101160070598 2315 14-15 0.6612300574437882 2315 16-17 0.6764903099056596 2315 18-19 0.7122839728248138 2315 20-21 0.696212541979115 2315 22-23 0.8020186704070795 2315 24-25 0.7578499488212813 2315 26-27 0.8451973576853575 2315 28-29 0.883072125457339 2315 30-31 0.848091811201023 2315 32-33 0.8905347181133081 2315 34-35 0.8781953105011695 2315 36-37 0.8640528861216339 2315 38-39 0.8992174132080066 2315 40-41 0.8755025471511928 2315 42-43 0.9330764274246732 2315 44-45 0.8349666854553419 2315 46-47 0.8665401600730362 2315 48-49 1.0330033153194833 2315 50-51 0.8995071652524373 2315 52-53 0.9183033685727295 2315 54-55 0.8730785132499435 2315 56-57 0.9871651602530918 2315 58-59 1.004422692620473 2315 60-61 1.0251070157406517 2315 62-63 1.008514235559474 2315 64-65 1.054690958728024 2315 66-67 1.0845259681434385 2315 68-69 1.0103222041741518 2315 70-71 0.6872708679896959 2315 72-73 0.9185406502197466 2315 74-75 1.0786519264101386 2315 76-77 1.056075407658323 2315 78-79 0.9405858538309886 2315 80-81 0.8911554559172501 2315 82-83 0.8617513601066094 2315 84-85 0.8106118158005664 2315 86-87 0.8190599624982227 2315 88-89 0.9038287096587645 2315 90-91 0.9635256272618626 2315 92-93 1.0745151800205832 2315 94-95 1.122237915341529 2315 96-97 1.2089828540396752 2315 98-99 1.1035949552706121 2315 100 1.0546167802214015 2316 1 0.48785797447339263 2316 2 0.4496994420067466 2316 3 0.46114755269852736 2316 4 0.28004918981253013 2316 5 0.3685067198620047 2316 6 0.3981378797578188 2316 7 0.45146386758702306 2316 8 0.42902108381858284 2316 9 0.4419143152531575 2316 10-11 0.4365190602464466 2316 12-13 0.6049936216100917 2316 14-15 0.7173539212361106 2316 16-17 0.7040704247435485 2316 18-19 0.7766863015107646 2316 20-21 0.8361421760097656 2316 22-23 0.7935527517952892 2316 24-25 0.8739500588258338 2316 26-27 0.9094043402277947 2316 28-29 0.847892018587693 2316 30-31 0.7223187929425094 2316 32-33 0.7118868959849962 2316 34-35 0.6301433609721698 2316 36-37 0.6078614162963092 2316 38-39 0.5607348984455527 2316 40-41 0.663704729168991 2316 42-43 0.8247615057295974 2316 44-45 0.781275928877804 2316 46-47 0.8020669201855597 2316 48-49 0.5803804418025251 2316 50-51 0.6656625635921003 2316 52-53 0.8481585077582423 2316 54-55 0.939192843406019 2316 56-57 0.956468623042646 2316 58-59 0.8009824533414402 2316 60-61 0.8929041178212813 2316 62-63 0.9115772444787424 2316 64-65 0.885827383194659 2316 66-67 0.9101749684833322 2316 68-69 0.8787359149092495 2316 70-71 0.7668525018954426 2316 72-73 0.8279778782455622 2316 74-75 0.8627122049638132 2316 76-77 0.7221064124136909 2316 78-79 0.6640882592285493 2316 80-81 0.6559887179200601 2316 82-83 0.7172706572244394 2316 84-85 0.7705350793687664 2316 86-87 0.749881286190984 2316 88-89 0.706883988136962 2316 90-91 0.8046867243462081 2316 92-93 0.796914231247662 2316 94-95 0.734759516978631 2316 96-97 0.8892193972649025 2316 98-99 0.9872452860873047 2316 100 0.6087807376723404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 2.0 12 30.0 13 140.0 14 614.0 15 1786.0 16 4317.0 17 8553.0 18 14271.0 19 20135.0 20 27690.0 21 37232.0 22 44785.0 23 48079.0 24 50878.0 25 56973.0 26 66343.0 27 80457.0 28 98992.0 29 120140.0 30 147159.0 31 183022.0 32 230596.0 33 300409.0 34 421712.0 35 576885.0 36 880168.0 37 1375979.0 38 1258104.0 39 199447.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78611350022338 19.717195577999792 13.453500392930545 24.043190528846285 2 15.647317913484175 20.802583564151448 40.10500525585839 23.445093266505992 3 18.01968848547345 26.850154078586076 30.812855469889726 24.317301966050753 4 12.309322538663958 17.401143693003032 38.582553652882794 31.706980115450218 5 12.684949509807057 38.00055318577449 34.7402425064112 14.574254798007253 6 29.65635878927405 37.67946058505897 18.83455037430505 13.829630251361927 7 26.495506087541525 32.992901891731066 23.067045292127492 17.444546728599917 8 25.94209045622363 35.27101103088414 20.621008225080747 18.16589028781148 9 27.049508470977663 15.60732548353512 20.495068721973382 36.84809732351384 10-11 24.77583492049484 25.94958302137676 29.03596665450016 20.23861540362824 12-13 25.313412309822088 23.968003003085823 28.560386941468845 22.15819774562325 14-15 22.80887208292795 25.51260618934828 26.363895577619772 25.31462615010399 16-17 22.101855651083017 27.879507021711948 27.224047052236717 22.794590274968325 18-19 21.799757790860802 27.52647713601682 28.84857931949624 21.82518575362614 20-21 22.84294923830803 26.817122543545096 28.291920756504858 22.048007461642015 22-23 22.561557492695755 26.683469426092238 28.268492427966763 22.48648065324524 24-25 22.308993006546928 26.546520208127312 28.562452782181197 22.582034003144564 26-27 22.325638541034 26.61659464073339 28.449502895559224 22.608263922673387 28-29 21.998095080503433 27.541962386832953 28.312083848433 22.147858684230613 30-31 22.963817407948763 26.614043691538075 28.451715042952515 21.970423857560643 32-33 22.81321548556401 26.771933802330235 28.155339805825243 22.259510906280518 34-35 22.909397357622822 26.859810408957603 28.380144751865366 21.850647481554212 36-37 22.53916781252289 26.807294330402563 28.426318006526824 22.227219850547726 38-39 22.466031553331106 26.894104540327707 28.447569169576507 22.192294736764676 40-41 22.445053210155532 26.38478700350045 28.77937589879319 22.390783887550832 42-43 22.304318165430793 26.75566112410467 28.11925192280052 22.82076878766402 44-45 21.808457185835476 26.975378356812723 28.559732645360253 22.656431811991546 46-47 21.929561993025278 26.413533077177682 28.28724290495218 23.36966202484486 48-49 22.18740317061573 26.531706956750583 28.302911252881497 22.977978619752186 50-51 21.959732732612213 26.817372987791842 28.25006823348114 22.9728260461148 52-53 21.75525663084575 27.146574688302223 27.891897492690404 23.20627118816163 54-55 21.567347235061156 26.70957203648398 28.24948697349578 23.473593754959083 56-57 22.040666072715457 26.596735506651175 27.81845237738365 23.544146043249718 58-59 21.622393183957396 26.749430522825374 28.186965667965108 23.441210625252122 60-61 22.01129608394163 26.97253998814515 27.853332368744276 23.162831559168946 62-63 21.94090091582261 26.83345780970455 27.6288222526181 23.59681902185474 64-65 21.45535047750612 26.665139035124785 27.650549327720856 24.228961159648236 66-67 21.558774956913958 26.491088887200547 27.501977359335577 24.44815879654992 68-69 21.262575627726626 26.66605347338668 27.638543356697333 24.432827542189365 70-71 21.517637664895243 26.35499935379107 27.67866397263657 24.448699008677114 72-73 21.5254929639864 26.530768594698973 27.401427595107297 24.54231084620733 74-75 21.547158752668174 26.720162735088117 27.159376358074766 24.573302154168946 76-77 21.38351935893372 26.38438133387664 27.205152595152278 25.02694671203736 78-79 21.238006420559863 26.38264889866732 27.41136953156136 24.967975149211462 80-81 21.215227677325956 26.463713039356207 27.284507325979508 25.036551957338332 82-83 21.502132068892426 26.102433818469063 27.52131569148173 24.874118421156787 84-85 21.581363008955424 26.36607641776062 26.93221536040863 25.12034521287533 86-87 21.044952986976696 26.530521495980818 27.22522192096828 25.19930359607421 88-89 21.1021225748609 25.992333682151354 27.23962406359638 25.665919679391365 90-91 21.469592479296587 25.64554778061939 27.31781388334952 25.56704585673451 92-93 21.41743724417862 25.83076407530818 27.183849331817004 25.56794934869619 94-95 20.828532754417893 25.626625441645267 27.4248719392612 26.119969864675635 96-97 20.775527761503337 25.465436326996254 27.609857266432876 26.149178645067533 98-99 20.683538053127627 25.675193135627737 27.36758749930239 26.273681311942244 100 20.649554434127584 25.524498727400097 27.415417319543383 26.410529518928932 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 609.0 1 435.5 2 662.5 3 1043.5 4 823.0 5 644.0 6 880.0 7 1622.0 8 2561.5 9 3148.5 10 3106.5 11 2969.5 12 3236.0 13 3783.5 14 4478.0 15 5046.5 16 5526.0 17 6133.5 18 6843.0 19 7537.5 20 8331.5 21 9425.5 22 10896.5 23 12960.0 24 15827.5 25 19547.5 26 24581.0 27 30930.0 28 38852.0 29 49723.0 30 62501.5 31 74936.0 32 90018.5 33 104529.0 34 117292.5 35 130322.0 36 144417.0 37 162651.0 38 173510.0 39 174840.0 40 176231.0 41 179580.5 42 185421.0 43 194408.5 44 206225.5 45 223386.5 46 239933.0 47 254324.5 48 272727.5 49 279009.5 50 275676.0 51 276749.0 52 272841.0 53 260442.0 54 243132.5 55 217222.5 56 191066.5 57 166014.5 58 139954.0 59 117276.5 60 92892.0 61 71179.0 62 54833.5 63 41500.5 64 29770.0 65 20453.5 66 14548.0 67 10910.0 68 8148.0 69 5990.5 70 4571.0 71 3401.5 72 2479.0 73 1792.5 74 1339.0 75 953.5 76 617.0 77 371.0 78 245.0 79 163.5 80 90.0 81 55.0 82 37.0 83 25.0 84 16.0 85 12.0 86 11.5 87 7.5 88 6.0 89 3.5 90 1.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05090388773646366 2 0.0 3 0.0 4 0.015571729477171987 5 0.003916913472183919 6 0.0 7 3.037606366183447E-4 8 5.115968616730016E-4 9 0.0025100221025831644 10-11 7.673952925095024E-4 12-13 4.076787491456732E-4 14-15 2.398110289092195E-5 16-17 7.194330867276585E-5 18-19 0.0 20-21 2.398110289092195E-5 22-23 0.0 24-25 5.595590674548455E-5 26-27 3.19748038545626E-5 28-29 0.0010791496300914878 30-31 8.393386011822685E-4 32-33 0.013877064872880172 34-35 0.02342154382346711 36-37 0.03131932037554407 38-39 0.02063174218715652 40-41 1.59874019272813E-4 42-43 0.001854538623564631 44-45 0.009760308876605235 46-47 0.01462847276346239 48-49 0.005867376507312238 50-51 0.010615634879714786 52-53 0.01583552160897213 54-55 0.009888208092023487 56-57 0.0032374488902744635 58-59 0.008057650571349776 60-61 0.0016387086975463334 62-63 9.032882088913937E-4 64-65 0.01975243508115605 66-67 0.04636346558911578 68-69 0.05591593824066635 70-71 0.04919323573024457 72-73 0.04143934579551314 74-75 0.029057103002833765 76-77 0.029464781751979443 78-79 0.012749953037006838 80-81 0.0020783622505465694 82-83 0.027602249427451167 84-85 0.048194023109789486 86-87 0.04945702786204471 88-89 0.060983944651614524 90-91 0.03536413306314624 92-93 0.0265470809002506 94-95 0.007530066307749493 96-97 0.0036691087423110593 98-99 0.02267013593288489 100 0.019056983097319312 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6254925.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.09095875039492 #Duplication Level Percentage of deduplicated Percentage of total 1 79.4457083142387 52.50643031092256 2 12.884667866251148 17.031201049218872 3 3.695460301494982 7.327095430494805 4 1.4733962248321255 3.8951267647347043 5 0.7597524944443876 2.5106385385416834 6 0.42999926480466516 1.7051438203743163 7 0.2843985535263149 1.315732114884576 8 0.19133551465969695 1.0116438085487691 9 0.1392440608753436 0.8282496135194833 >10 0.6551833506121352 7.358125460037618 >50 0.027229417995953 1.2203764944765616 >100 0.01201259498750814 1.4552099868953736 >500 0.0010181351198526166 0.437512023437005 >1k 4.5249992938571225E-4 0.5037317841999557 >5k 8.484373673348814E-5 0.36201399608629126 >10k+ 5.656249115565876E-5 0.5317688036273743 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15731 0.2514978197180622 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12719 0.20334376511309088 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8485 0.13565310535298186 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.796220578184391E-5 2 0.0 0.0 0.0 0.0 4.796220578184391E-5 3 0.0 0.0 0.0 0.0 4.796220578184391E-5 4 0.0 0.0 0.0 0.0 4.796220578184391E-5 5 0.0 0.0 0.0 1.59874019272813E-5 6.39496077091252E-5 6 0.0 0.0 0.0 1.59874019272813E-5 7.993700963640652E-5 7 0.0 0.0 0.0 1.59874019272813E-5 7.993700963640652E-5 8 0.0 0.0 0.0 1.59874019272813E-5 1.1191181349096912E-4 9 0.0 0.0 0.0 4.796220578184391E-5 1.1191181349096912E-4 10-11 3.19748038545626E-5 0.0 0.0 6.39496077091252E-5 1.1990551445460976E-4 12-13 3.19748038545626E-5 0.0 0.0 8.793071060004715E-5 3.0376063661834477E-4 14-15 3.19748038545626E-5 0.0 0.0 1.1191181349096912E-4 3.3573544047290737E-4 16-17 3.19748038545626E-5 0.0 0.0 1.5188031830917239E-4 3.5172284240018867E-4 18-19 3.19748038545626E-5 0.0 0.0 2.557984308365008E-4 3.6771024432747E-4 20-21 3.19748038545626E-5 1.59874019272813E-5 0.0 4.076787491456732E-4 4.316598520365952E-4 22-23 3.19748038545626E-5 1.59874019272813E-5 0.0 0.0013669228647825514 4.716283568547984E-4 24-25 3.19748038545626E-5 1.59874019272813E-5 0.0 0.003773026854838387 4.796220578184391E-4 26-27 3.19748038545626E-5 1.59874019272813E-5 0.0 0.007498091503894931 4.796220578184391E-4 28-29 3.19748038545626E-5 1.59874019272813E-5 0.0 0.019688485473446926 4.796220578184391E-4 30-31 3.19748038545626E-5 1.59874019272813E-5 0.0 0.06274255886361547 4.796220578184391E-4 32-33 3.19748038545626E-5 1.59874019272813E-5 0.0 0.16303952485441472 5.115968616730016E-4 34-35 3.19748038545626E-5 1.59874019272813E-5 0.0 0.3071979280327102 5.51565366491205E-4 36-37 3.19748038545626E-5 1.59874019272813E-5 0.0 0.5279120053397922 5.595590674548456E-4 38-39 3.19748038545626E-5 3.19748038545626E-5 0.0 0.8786835973253077 6.075212732366895E-4 40-41 3.19748038545626E-5 3.19748038545626E-5 0.0 1.2890242488918733 6.075212732366895E-4 42-43 3.19748038545626E-5 4.796220578184391E-5 0.0 1.679028925206937 6.235086751639708E-4 44-45 3.19748038545626E-5 4.796220578184391E-5 0.0 2.1200174262681006 6.315023761276114E-4 46-47 3.19748038545626E-5 4.796220578184391E-5 0.0 2.6148594907213116 6.394960770912521E-4 48-49 3.19748038545626E-5 4.796220578184391E-5 0.0 3.141540466112703 6.714708809458147E-4 50-51 3.19748038545626E-5 4.796220578184391E-5 0.0 3.694424793262909 6.714708809458147E-4 52-53 3.19748038545626E-5 4.796220578184391E-5 0.0 4.244719161300894 7.514078905822212E-4 54-55 3.19748038545626E-5 6.394960770912522E-5 0.0 4.835181876681175 7.993700963640652E-4 56-57 3.19748038545626E-5 7.993700963640652E-5 0.0 5.475661178990955 8.393386011822684E-4 58-59 3.19748038545626E-5 7.993700963640652E-5 0.0 6.175845433798167 8.633197040731904E-4 60-61 3.19748038545626E-5 7.993700963640652E-5 0.0 6.872744277509323 8.793071060004716E-4 62-63 3.19748038545626E-5 7.993700963640652E-5 0.0 7.587413118462651 9.272693117823156E-4 64-65 3.19748038545626E-5 7.993700963640652E-5 0.0 8.257477108038866 9.592441156368782E-4 66-67 3.19748038545626E-5 7.993700963640652E-5 0.0 8.982905470489253 9.832252185278001E-4 68-69 3.19748038545626E-5 7.993700963640652E-5 0.0 9.786224455129357 0.001007206321418722 70-71 3.19748038545626E-5 7.993700963640652E-5 0.0 10.614363881261566 0.001031187424309644 72-73 4.796220578184391E-5 8.793071060004717E-5 0.0 11.387898336111146 0.0010871433310551287 74-75 4.796220578184391E-5 9.592441156368782E-5 0.0 12.136916110105236 0.0011271118358733318 76-77 4.796220578184391E-5 9.592441156368782E-5 0.0 12.919555390352402 0.0011510929387642537 78-79 4.796220578184391E-5 9.592441156368782E-5 0.0 13.767950854726475 0.0011510929387642537 80-81 4.796220578184391E-5 9.592441156368782E-5 0.0 14.612517336338964 0.0011590866397278945 82-83 4.796220578184391E-5 9.592441156368782E-5 0.0 15.462839282645275 0.0012310299484006603 84-85 4.796220578184391E-5 9.592441156368782E-5 0.0 16.282985007813842 0.0012310299484006603 86-87 4.796220578184391E-5 9.592441156368782E-5 0.0 17.0840497687822 0.001286985855146145 88 4.796220578184391E-5 9.592441156368782E-5 0.0 17.711035703865353 0.0013429417618916295 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 5515 0.0 41.83697 2 ACCTGGG 6775 0.0 33.294315 3 GGTATCA 15040 0.0 32.917515 1 GTATCAA 22985 0.0 32.09408 1 GTACATG 21080 0.0 27.277363 1 CAACGCA 27130 0.0 26.550425 5 ATCAACG 27050 0.0 26.545702 3 TATCAAC 28135 0.0 26.155865 2 TACATGG 21545 0.0 26.107897 2 TCAACGC 27865 0.0 25.803225 4 TGGGGGG 12920 0.0 25.78392 6 GTACCTG 9200 0.0 25.705538 1 AACGCAG 28705 0.0 25.485683 6 ACATGGG 21110 0.0 25.400106 3 ATGGGGG 12455 0.0 24.48387 5 CATGGGG 16635 0.0 23.730017 4 CCTGGGG 6715 0.0 23.584435 4 ACGCAGA 31110 0.0 23.122799 7 CGCAGAG 31650 0.0 22.668903 8 CTGGGGG 4745 0.0 22.379549 5 >>END_MODULE