FastQCFastQC Report
Mon 6 Jun 2016
ERR1211435_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211435_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9252859
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361630.39083055302150393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275660.2979187297677399No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185040.19998143276580785No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG98410.10635631646391672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159300.055.516751
TACCTGG84000.055.083212
ACCTGGG96700.046.962423
GTATCAA304600.043.713721
CCTGGGG89550.036.9574664
ATCAACG381700.034.128263
GTACATG426450.034.095991
CAACGCA384500.033.8793685
TATCAAC394500.033.4680862
TACATGG433050.033.2061232
TCAACGC405200.032.2878344
AACGCAG412600.032.074396
ACATGGG434800.031.7875943
CTGGGGG72000.030.8833075
CATGGGG335200.030.8261174
ATGGGGG279150.030.5151625
TGGGGGG303150.030.5039466
TAACGCA10450.030.140724
TATCACG6350.029.650452
GTACCTG158850.029.4832061
Produced by FastQC (version 0.11.5)