FastQCFastQC Report
Mon 6 Jun 2016
ERR1211435_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211435_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9252859
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321130.3470602977955246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258430.2792974582234529No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179820.1943399332033483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG90250.056.4453742
GGTATCA161150.053.1900441
ACCTGGG99750.049.9873923
GTATCAA297600.042.026451
CCTGGGG87450.039.499594
CTGGGGG68300.035.637215
TAACGCA10600.035.4690174
ATCAACG375800.032.576843
CAACGCA379850.032.2371675
TGGGGGG270350.032.0659036
GTACATG422200.031.7729511
TATCAAC391700.031.6015572
TACATGG429050.030.8549752
TCAACGC399150.030.7304844
AACGCAG406900.030.5429846
GTACCTG169650.030.237431
ACATGGG423800.029.8851763
ATGGGGG257500.029.6348955
CATGGGG316900.029.3486824
ATAACGC14000.028.5330943
Produced by FastQC (version 0.11.5)