##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211435_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9252859 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06171670831686 33.0 31.0 34.0 30.0 34.0 2 32.19348236042503 33.0 31.0 34.0 30.0 34.0 3 32.26594007322493 34.0 31.0 34.0 30.0 34.0 4 35.9204289182403 37.0 35.0 37.0 35.0 37.0 5 35.78596572151375 37.0 35.0 37.0 35.0 37.0 6 35.82239521860216 37.0 35.0 37.0 35.0 37.0 7 35.75595953639843 37.0 35.0 37.0 33.0 37.0 8 35.73393812658336 37.0 35.0 37.0 33.0 37.0 9 37.3161437994462 39.0 37.0 39.0 34.0 39.0 10-11 37.285625772531496 39.0 37.0 39.0 34.0 39.0 12-13 37.19128768740559 39.0 37.0 39.0 33.0 39.0 14-15 38.37250843225861 40.0 38.0 41.0 33.0 41.0 16-17 38.35826532102132 40.0 38.0 41.0 33.0 41.0 18-19 38.380654833279095 40.0 38.0 41.0 33.5 41.0 20-21 38.343573375537225 40.0 38.0 41.0 34.0 41.0 22-23 38.24260220543726 40.0 38.0 41.0 34.0 41.0 24-25 38.13046194695067 40.0 37.0 41.0 33.0 41.0 26-27 37.99927044170888 40.0 37.0 41.0 33.0 41.0 28-29 37.90734561069179 40.0 37.0 41.0 33.0 41.0 30-31 37.94949123292595 40.0 37.0 41.0 33.0 41.0 32-33 37.76706145635635 40.0 37.0 41.0 33.0 41.0 34-35 37.66337609813356 40.0 37.0 41.0 33.0 41.0 36-37 37.53797561380758 40.0 36.0 41.0 32.5 41.0 38-39 37.42573273838929 40.0 36.0 41.0 32.0 41.0 40-41 37.36109758075855 40.0 36.0 41.0 32.0 41.0 42-43 37.232865052844744 40.0 36.0 41.0 31.0 41.0 44-45 37.086273874918014 39.0 36.0 41.0 31.0 41.0 46-47 36.86176834641056 39.0 35.5 41.0 30.5 41.0 48-49 36.642436029771986 39.0 35.0 41.0 30.0 41.0 50-51 36.46055975780027 39.0 35.0 41.0 30.0 41.0 52-53 36.26704281346987 39.0 35.0 40.0 29.0 41.0 54-55 36.07305460939154 38.5 35.0 40.0 29.0 41.0 56-57 35.84672440161468 38.0 35.0 40.0 28.0 41.0 58-59 35.59590273665685 38.0 34.0 40.0 28.0 41.0 60-61 35.33364109406617 38.0 34.0 40.0 27.5 41.0 62-63 35.015761831018935 37.0 34.0 40.0 27.0 41.0 64-65 34.66849419190328 36.5 34.0 39.5 26.0 41.0 66-67 34.32712851238736 36.0 33.0 39.0 26.0 41.0 68-69 33.967254553430465 36.0 33.0 39.0 26.0 40.0 70-71 33.59614849853435 35.0 33.0 38.5 25.5 40.0 72-73 33.23477505709316 35.0 32.0 37.5 25.0 40.0 74-75 32.85254422443917 35.0 32.0 37.0 24.5 39.0 76-77 31.831424806105872 34.0 30.5 36.0 23.5 38.5 78-79 32.31383305419438 35.0 31.5 36.0 24.5 38.5 80-81 32.214282688194 35.0 32.0 36.0 24.5 37.5 82-83 31.927029256578965 34.5 31.0 35.0 24.0 37.0 84-85 31.634606179560286 34.0 31.0 35.0 23.5 36.5 86-87 31.383749336286222 34.0 31.0 35.0 23.0 36.0 88-89 31.159670702860595 34.0 31.0 35.0 21.5 36.0 90-91 30.934452908014702 34.0 30.5 35.0 20.0 35.0 92-93 30.735450902256265 34.0 30.0 35.0 20.0 35.0 94-95 30.553882535116983 34.0 30.0 35.0 20.0 35.0 96-97 30.379722851066894 34.0 30.0 35.0 19.0 35.0 98-99 30.188072518991156 34.0 29.0 35.0 19.0 35.0 100 28.372880533465388 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.24798977943024525 1101 2 -0.2770303623066006 1101 3 -0.29862372986611874 1101 4 -0.1644508590713727 1101 5 -0.16645194830862664 1101 6 -0.266612313065707 1101 7 -0.2663132962578487 1101 8 -0.2972978267505866 1101 9 -0.5026341859728447 1101 10-11 -0.43048782841464117 1101 12-13 -0.48721516088443906 1101 14-15 -0.6529101322231412 1101 16-17 -0.6560789742630959 1101 18-19 -0.6951340818680336 1101 20-21 -0.6508161201619558 1101 22-23 -0.7298084559472784 1101 24-25 -0.7868998015757214 1101 26-27 -0.7836001613104528 1101 28-29 -0.857530788557689 1101 30-31 -0.7348397249074523 1101 32-33 -0.5977947745122734 1101 34-35 -0.6505067612383684 1101 36-37 -0.7240798870642848 1101 38-39 -0.6873305911877381 1101 40-41 -0.6922300789893541 1101 42-43 -0.6911218223509294 1101 44-45 -0.7651949169967978 1101 46-47 -0.7981987192684485 1101 48-49 -0.7233517956339952 1101 50-51 -0.8537041671644232 1101 52-53 -0.8105833659885704 1101 54-55 -0.8121076095624744 1101 56-57 -0.8123337910562256 1101 58-59 -0.8396685003416664 1101 60-61 -0.8861272684885435 1101 62-63 -0.8527788838039214 1101 64-65 -0.819945230062757 1101 66-67 -0.8628933339664968 1101 68-69 -0.8038794672045526 1101 70-71 -0.9000523994065119 1101 72-73 -0.8781601778586392 1101 74-75 -1.0489178075558385 1101 76-77 -0.8928735760763189 1101 78-79 -0.778754702728488 1101 80-81 -0.6582181275706063 1101 82-83 -0.7251963752623887 1101 84-85 -0.8606890630546395 1101 86-87 -0.9753078872843979 1101 88-89 -0.9669613108984478 1101 90-91 -1.0736843954051132 1101 92-93 -1.1871150429045514 1101 94-95 -1.234735204375152 1101 96-97 -1.2602471777739126 1101 98-99 -1.446743164703264 1101 100 -1.2907575509553162 1102 1 -0.34454723620745753 1102 2 -0.3060744114661773 1102 3 -0.3537325243996392 1102 4 -0.20203066803338032 1102 5 -0.19437108216121857 1102 6 -0.3202073063929731 1102 7 -0.30798401270233455 1102 8 -0.2965854794478915 1102 9 -0.5134727164636104 1102 10-11 -0.4690140637523612 1102 12-13 -0.45208540048836454 1102 14-15 -0.6049874855760322 1102 16-17 -0.6424346428085386 1102 18-19 -0.6764657894386374 1102 20-21 -0.7470538838396408 1102 22-23 -0.7094317968155295 1102 24-25 -0.6992858932929593 1102 26-27 -0.7884894283981723 1102 28-29 -0.7205707185321941 1102 30-31 -0.6672399526662005 1102 32-33 -0.7081945442229554 1102 34-35 -0.7429555336360139 1102 36-37 -0.6596870486737672 1102 38-39 -0.7358147687306698 1102 40-41 -0.7681168340429707 1102 42-43 -0.8771197808186812 1102 44-45 -0.8010510562661963 1102 46-47 -0.8651136865257953 1102 48-49 -0.7776597030413726 1102 50-51 -0.8377732564736746 1102 52-53 -0.9057636302352208 1102 54-55 -0.9558426764136456 1102 56-57 -0.9114714637263077 1102 58-59 -0.8902957322288003 1102 60-61 -0.93328749994145 1102 62-63 -0.9681754398100537 1102 64-65 -0.8866162302789107 1102 66-67 -0.875131161307074 1102 68-69 -1.0288500977945176 1102 70-71 -1.0489715648301328 1102 72-73 -1.0656054177777463 1102 74-75 -1.0380958361566215 1102 76-77 -0.8544971614554129 1102 78-79 -0.7454979195784937 1102 80-81 -0.7308218167799581 1102 82-83 -0.7610451694028413 1102 84-85 -0.8771319499130463 1102 86-87 -0.9398180470682966 1102 88-89 -0.8717999953994848 1102 90-91 -0.9764952345605913 1102 92-93 -0.9758095766687767 1102 94-95 -1.0354653025337974 1102 96-97 -1.1074660216708345 1102 98-99 -1.145330543988674 1102 100 -1.1658231573560656 1103 1 -0.3774792315004447 1103 2 -0.3308764669737414 1103 3 -0.3624793791237124 1103 4 -0.18872005506494105 1103 5 -0.20863402420769006 1103 6 -0.2830207599542547 1103 7 -0.341686402039322 1103 8 -0.3672760333224545 1103 9 -0.45281259259156315 1103 10-11 -0.501766447862181 1103 12-13 -0.5863810494614228 1103 14-15 -0.7628499733651353 1103 16-17 -0.8533727449717077 1103 18-19 -0.6722897844522322 1103 20-21 -0.7015813772667769 1103 22-23 -0.811491293904524 1103 24-25 -0.7929415885199518 1103 26-27 -0.8507088187207543 1103 28-29 -0.8483767141064149 1103 30-31 -0.8179784207474228 1103 32-33 -0.7808349514048061 1103 34-35 -0.8478496640130118 1103 36-37 -0.7598430645247092 1103 38-39 -0.794595092154637 1103 40-41 -0.8452691101281999 1103 42-43 -0.8253231539916612 1103 44-45 -0.837102693911639 1103 46-47 -0.7878317770436283 1103 48-49 -0.8381573897377947 1103 50-51 -0.8218445781254715 1103 52-53 -0.9086849731457534 1103 54-55 -0.877583190338207 1103 56-57 -0.9922596968489685 1103 58-59 -1.0653384194951556 1103 60-61 -1.0439313471122418 1103 62-63 -1.0064889414750482 1103 64-65 -0.9971519659103265 1103 66-67 -0.9905313539887928 1103 68-69 -0.9347717465688135 1103 70-71 -0.9357509193732128 1103 72-73 -0.9779674992902514 1103 74-75 -1.0660664473292485 1103 76-77 -0.9326440457118856 1103 78-79 -0.7520046120516 1103 80-81 -0.7693915192549312 1103 82-83 -0.8061116467551201 1103 84-85 -0.8249644664727676 1103 86-87 -0.8830526297833181 1103 88-89 -0.8125095192434912 1103 90-91 -0.9571615974474774 1103 92-93 -0.9833104800095782 1103 94-95 -0.9596472766684983 1103 96-97 -0.9960008431442908 1103 98-99 -1.1055078286223115 1103 100 -1.0715232381945903 1104 1 -0.40343514924228785 1104 2 -0.3567188821530536 1104 3 -0.3726947690228961 1104 4 -0.21096470398163092 1104 5 -0.2169986229915537 1104 6 -0.31627150867443987 1104 7 -0.3307896347845869 1104 8 -0.3370519037693924 1104 9 -0.5428923759593474 1104 10-11 -0.45750520489690416 1104 12-13 -0.4873510048017309 1104 14-15 -0.6681130230467929 1104 16-17 -0.6718288060577748 1104 18-19 -0.7383815925180741 1104 20-21 -0.673428144608387 1104 22-23 -0.7188296711204885 1104 24-25 -0.7865253635175904 1104 26-27 -0.7977767777828007 1104 28-29 -0.8475395331631361 1104 30-31 -0.7440910470278936 1104 32-33 -0.6842862748918819 1104 34-35 -0.7421808198655384 1104 36-37 -0.6906673502795329 1104 38-39 -0.8378986798723318 1104 40-41 -0.8334638228403151 1104 42-43 -0.6878530101319811 1104 44-45 -0.7655987798903752 1104 46-47 -0.7943569364163423 1104 48-49 -0.8164322622772673 1104 50-51 -0.7358089334847762 1104 52-53 -0.6580634890610142 1104 54-55 -0.6737268542629025 1104 56-57 -0.6600664809980472 1104 58-59 -0.8682547649645826 1104 60-61 -0.8843990069178815 1104 62-63 -0.9231058382550472 1104 64-65 -0.7599927269841729 1104 66-67 -0.8266378092280391 1104 68-69 -0.7469478103422205 1104 70-71 -0.8169309377096923 1104 72-73 -0.760544790785211 1104 74-75 -0.7185047325732512 1104 76-77 -0.585321549298385 1104 78-79 -0.631774550364355 1104 80-81 -0.7400560732111501 1104 82-83 -0.7999174532676427 1104 84-85 -0.7085515166687308 1104 86-87 -0.6685195525969405 1104 88-89 -0.8219915079693969 1104 90-91 -0.7886384252021621 1104 92-93 -0.8520621270202327 1104 94-95 -0.9609556619291233 1104 96-97 -1.0141712510469318 1104 98-99 -1.0132336768581318 1104 100 -1.1336154673159982 1105 1 -0.36451915903383636 1105 2 -0.34424320375096684 1105 3 -0.4198605617357991 1105 4 -0.21410297647594234 1105 5 -0.23999374391895145 1105 6 -0.30262501151342036 1105 7 -0.32990918598116536 1105 8 -0.31678550607153255 1105 9 -0.5150210495296861 1105 10-11 -0.5093223379351457 1105 12-13 -0.5106493210651806 1105 14-15 -0.6896209983040436 1105 16-17 -0.5953248892714598 1105 18-19 -0.6046111477619931 1105 20-21 -0.7222691769742795 1105 22-23 -0.6850108267325083 1105 24-25 -0.7077589953257615 1105 26-27 -0.6804419572713201 1105 28-29 -0.7757906731815041 1105 30-31 -0.7324689558074979 1105 32-33 -0.6487257744473496 1105 34-35 -0.6149242070353154 1105 36-37 -0.7560314532990091 1105 38-39 -0.7063222937708886 1105 40-41 -0.6893733726618052 1105 42-43 -0.7491010497903048 1105 44-45 -0.710483025785841 1105 46-47 -0.8721689724270547 1105 48-49 -0.746677787242433 1105 50-51 -0.8399953589851137 1105 52-53 -0.8354107007133393 1105 54-55 -0.8365330558737085 1105 56-57 -0.8489553868977495 1105 58-59 -0.879273986751393 1105 60-61 -0.8224360584192638 1105 62-63 -0.8496031718563444 1105 64-65 -0.8644003572435466 1105 66-67 -0.9253953130846995 1105 68-69 -0.831100260762355 1105 70-71 -0.9100722192623536 1105 72-73 -0.9242367996058434 1105 74-75 -0.9581256107061122 1105 76-77 -0.911290063859056 1105 78-79 -0.8211998744992286 1105 80-81 -0.7077062394191707 1105 82-83 -0.7664229455646208 1105 84-85 -0.709370441224042 1105 86-87 -0.7207188374375768 1105 88-89 -0.7513849836850959 1105 90-91 -0.7773586596025197 1105 92-93 -0.7946987789097584 1105 94-95 -0.7681658640008884 1105 96-97 -0.8546604624175522 1105 98-99 -0.8890986071598803 1105 100 -0.9169700858169456 1106 1 -0.38501340467637135 1106 2 -0.36508027092719075 1106 3 -0.39769102829745506 1106 4 -0.23083142572912863 1106 5 -0.2801808581237921 1106 6 -0.30608429349815225 1106 7 -0.32104206327438334 1106 8 -0.3646632011939346 1106 9 -0.45298298283512395 1106 10-11 -0.4805658420562935 1106 12-13 -0.5172871766408988 1106 14-15 -0.64066704942271 1106 16-17 -0.7149971842418665 1106 18-19 -0.6811207631179315 1106 20-21 -0.6567838545276032 1106 22-23 -0.6221961207166515 1106 24-25 -0.7875979623242415 1106 26-27 -0.7332674796404817 1106 28-29 -0.7851881863393402 1106 30-31 -0.7566566859166883 1106 32-33 -0.79112616425315 1106 34-35 -0.7144296789442848 1106 36-37 -0.7296521472123558 1106 38-39 -0.7790994771347712 1106 40-41 -0.7456953038097822 1106 42-43 -0.7335012859828325 1106 44-45 -0.8083156823311768 1106 46-47 -0.915015960076154 1106 48-49 -0.8719693897904648 1106 50-51 -0.8810824049105079 1106 52-53 -0.9202038372846104 1106 54-55 -0.9611194710467217 1106 56-57 -0.9837759849344891 1106 58-59 -0.9607394849143631 1106 60-61 -1.046428705567216 1106 62-63 -0.985610866549024 1106 64-65 -1.0255816039099628 1106 66-67 -0.9231553835898652 1106 68-69 -1.0545671801438345 1106 70-71 -0.9525803493315337 1106 72-73 -0.8500748526643989 1106 74-75 -0.9647025020166211 1106 76-77 -0.8980433634743257 1106 78-79 -0.7954341481442029 1106 80-81 -0.7471795664848884 1106 82-83 -0.7302272774969154 1106 84-85 -0.7446610781798135 1106 86-87 -0.764279901124123 1106 88-89 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1107 46-47 -0.8311313422910231 1107 48-49 -0.8784485862433158 1107 50-51 -0.8041389899959484 1107 52-53 -0.7641167529467197 1107 54-55 -0.8120817952267174 1107 56-57 -0.8561204949139096 1107 58-59 -0.8744881295091318 1107 60-61 -0.8729162987164543 1107 62-63 -0.8889912635408308 1107 64-65 -0.8512782573275572 1107 66-67 -0.9027625875554222 1107 68-69 -0.8095563778753672 1107 70-71 -0.8377656278591488 1107 72-73 -0.8631084792894157 1107 74-75 -0.7314981109546608 1107 76-77 -0.8263465309079692 1107 78-79 -0.7212053575462818 1107 80-81 -0.7229503274996638 1107 82-83 -0.6240521159782801 1107 84-85 -0.68110491027495 1107 86-87 -0.6983387766440288 1107 88-89 -0.593117882446375 1107 90-91 -0.584409109218992 1107 92-93 -0.6196893972627713 1107 94-95 -0.670933438615517 1107 96-97 -0.7271705682792593 1107 98-99 -0.7194658135953027 1107 100 -0.7814175853371133 1108 1 -0.42915382261139 1108 2 -0.3910671461887212 1108 3 -0.44608961293144844 1108 4 -0.2657435970574724 1108 5 -0.342171277094522 1108 6 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0.844679419713934 2311 32-33 0.9799231069964023 2311 34-35 0.9619358165072498 2311 36-37 1.0173383670667633 2311 38-39 1.053062302877244 2311 40-41 1.0560273990729883 2311 42-43 1.1629381820712723 2311 44-45 1.1005890717271782 2311 46-47 1.138551125904705 2311 48-49 1.1630294074360847 2311 50-51 1.1483996515947226 2311 52-53 1.1844298748299877 2311 54-55 1.178943309280747 2311 56-57 1.192298743715007 2311 58-59 1.21837333485945 2311 60-61 1.2167510735846676 2311 62-63 1.2350638195258483 2311 64-65 1.252096527276322 2311 66-67 1.3071450129151643 2311 68-69 1.3039230789569274 2311 70-71 1.3340600567292995 2311 72-73 1.2781650341590307 2311 74-75 1.2184829474871535 2311 76-77 1.1073250894659168 2311 78-79 1.0816592835854522 2311 80-81 1.1085756590662896 2311 82-83 1.0933684758892497 2311 84-85 1.1214803200047108 2311 86-87 1.176730104786099 2311 88-89 1.1786394197149654 2311 90-91 1.2786879602180257 2311 92-93 1.3565008050890626 2311 94-95 1.3318249585567976 2311 96-97 1.3261379594139449 2311 98-99 1.4049846586958843 2311 100 1.4614544161661236 2312 1 0.16199847966209546 2312 2 0.17161935593252053 2312 3 0.33702555050421523 2312 4 0.25062514592111285 2312 5 0.3629827655450484 2312 6 0.4415959937509015 2312 7 0.48418225842055307 2312 8 0.31216447931593905 2312 9 0.4427511848162524 2312 10-11 0.29464696611901076 2312 12-13 0.47786486384354987 2312 14-15 0.8376923024927052 2312 16-17 0.8691875955272295 2312 18-19 0.9038522368140818 2312 20-21 0.907730729690698 2312 22-23 0.5490589185246861 2312 24-25 0.5009935854991454 2312 26-27 0.8231394077242342 2312 28-29 0.9658800366448617 2312 30-31 0.8696522397157764 2312 32-33 0.7217906495676232 2312 34-35 0.8806007423575934 2312 36-37 0.9889117086568362 2312 38-39 0.9586793567596672 2312 40-41 0.9962937776990657 2312 42-43 0.8017425494635049 2312 44-45 0.8062815642474348 2312 46-47 0.9456297740650399 2312 48-49 0.969039732632254 2312 50-51 1.0326828683530067 2312 52-53 1.1055956668978268 2312 54-55 1.120228617630083 2312 56-57 1.0954378850570592 2312 58-59 1.115763882007677 2312 60-61 1.0891656735615811 2312 62-63 1.1114322463095192 2312 64-65 1.1762045089362374 2312 66-67 1.1210862154948913 2312 68-69 1.0998751990465792 2312 70-71 0.9801621910633997 2312 72-73 1.0933047529259667 2312 74-75 1.1413853061401724 2312 76-77 1.0581380096202366 2312 78-79 0.963901813362078 2312 80-81 0.8345102858289906 2312 82-83 0.8799741110114994 2312 84-85 0.7302070312118936 2312 86-87 0.8758394708831823 2312 88-89 0.5244363338680138 2312 90-91 0.9745067126489211 2312 92-93 1.1275905689568795 2312 94-95 1.20795658560181 2312 96-97 1.1867020092930503 2312 98-99 1.2105982680496545 2312 100 1.2980723958004248 2313 1 -0.3842778665421136 2313 2 -0.03841234602947452 2313 3 0.22902754952963278 2313 4 0.11633655637411522 2313 5 0.22405253742807218 2313 6 0.3529355486643979 2313 7 0.09508139990034437 2313 8 0.09625642045864424 2313 9 0.26904603673688143 2313 10-11 0.41436192647633163 2313 12-13 0.5652494084395201 2313 14-15 0.6820992283916425 2313 16-17 0.8028816585656955 2313 18-19 0.706411090524071 2313 20-21 0.8779868542353597 2313 22-23 0.8833900392065885 2313 24-25 0.7053114990296194 2313 26-27 0.9055387375480493 2313 28-29 0.8531787496891923 2313 30-31 0.822971982794769 2313 32-33 0.9720476123526609 2313 34-35 0.9989941109098837 2313 36-37 0.8675229311148058 2313 38-39 0.6477353729438065 2313 40-41 0.8086687431841497 2313 42-43 0.7936978244743713 2313 44-45 0.9524850920950954 2313 46-47 1.0350111964673303 2313 48-49 1.0623643326732264 2313 50-51 0.9489668291890538 2313 52-53 0.93804481548068 2313 54-55 1.0601520515203262 2313 56-57 1.156689669694451 2313 58-59 1.0270668058508008 2313 60-61 1.0389231299873032 2313 62-63 1.0684603263382542 2313 64-65 1.1532539248332085 2313 66-67 1.118965581465055 2313 68-69 1.1422498895364726 2313 70-71 1.0679156721560048 2313 72-73 1.127981402796003 2313 74-75 0.7530778817214383 2313 76-77 0.8914701335228301 2313 78-79 0.7458314936866302 2313 80-81 0.8397425264795473 2313 82-83 0.8817307707061985 2313 84-85 0.9812106503935247 2313 86-87 1.0429267230307708 2313 88-89 1.0403682698242385 2313 90-91 1.1609442931120704 2313 92-93 0.91001231701253 2313 94-95 0.9496830223354742 2313 96-97 1.2307437301736748 2313 98-99 1.248090913102864 2313 100 1.3593380686175962 2314 1 -0.33966148713566113 2314 2 -0.13145458445956848 2314 3 0.1396552835536511 2314 4 -0.16335511772992106 2314 5 0.18410558028117663 2314 6 0.21800530525904804 2314 7 0.1459843396748255 2314 8 0.18911546157740133 2314 9 0.23925477922594496 2314 10-11 0.4629074061128051 2314 12-13 0.5559292452251796 2314 14-15 0.6114835950321336 2314 16-17 0.7982417648201334 2314 18-19 0.8452925805449425 2314 20-21 0.47556593792339186 2314 22-23 0.7143233270619902 2314 24-25 0.9006364422474888 2314 26-27 0.8557795757239504 2314 28-29 0.8195049445544882 2314 30-31 0.7889680910571499 2314 32-33 0.8881158244252347 2314 34-35 0.9049155120318488 2314 36-37 0.7938042426388208 2314 38-39 0.8425384448734619 2314 40-41 0.8919534649502197 2314 42-43 0.7297914913312695 2314 44-45 0.747583268377916 2314 46-47 0.8572588828479937 2314 48-49 0.7159596100393841 2314 50-51 0.8428249284084828 2314 52-53 0.9172745189963223 2314 54-55 0.9520111004731788 2314 56-57 0.8147393836595782 2314 58-59 0.8405235824338462 2314 60-61 0.947065647853556 2314 62-63 0.6669880888663187 2314 64-65 0.815097148913047 2314 66-67 0.8610526929918407 2314 68-69 0.9628516625730867 2314 70-71 1.0261099251840307 2314 72-73 1.017230191373649 2314 74-75 1.0037321793556018 2314 76-77 0.8288999656381648 2314 78-79 0.8170880163244547 2314 80-81 0.8277021242409219 2314 82-83 0.8116712074751007 2314 84-85 0.7552958238856 2314 86-87 0.9101978604751473 2314 88-89 0.9909420570610514 2314 90-91 1.0301704893633925 2314 92-93 1.1546694638469823 2314 94-95 1.1880572245383014 2314 96-97 1.2584707035070153 2314 98-99 1.2618332725998656 2314 100 1.2602608648111868 2315 1 -0.0401188699728543 2315 2 0.14808503574334253 2315 3 0.11469877898134939 2315 4 -0.049275806638078734 2315 5 0.26521991130309175 2315 6 0.3950135181618819 2315 7 0.4368252639284549 2315 8 0.43753811269055376 2315 9 0.6195177435194026 2315 10-11 0.5894851785975277 2315 12-13 0.6602646651424706 2315 14-15 0.7799611927489494 2315 16-17 0.7223553849870186 2315 18-19 0.7810859760015489 2315 20-21 0.7765028917537435 2315 22-23 0.9114294467678263 2315 24-25 0.8691500718965841 2315 26-27 0.9662938034414168 2315 28-29 0.9705163403776709 2315 30-31 0.9260969229443887 2315 32-33 0.9336496369558702 2315 34-35 0.9176197921532037 2315 36-37 0.9040604324761077 2315 38-39 0.9553758108731145 2315 40-41 0.9410861011728997 2315 42-43 0.9943478110976898 2315 44-45 0.877161321764838 2315 46-47 0.8880370135119264 2315 48-49 0.9467694844298009 2315 50-51 0.8685833226809692 2315 52-53 0.9693456087731747 2315 54-55 0.8542925104783095 2315 56-57 0.97022408060554 2315 58-59 0.9863190672146729 2315 60-61 1.0069996349998007 2315 62-63 1.05535631628495 2315 64-65 1.0652189038972253 2315 66-67 1.057153089697124 2315 68-69 1.0124239634810266 2315 70-71 0.6503333506891025 2315 72-73 0.8922879212844137 2315 74-75 1.028958821756568 2315 76-77 1.0202552569081647 2315 78-79 0.9097234677925101 2315 80-81 0.9230183940501391 2315 82-83 0.9157163282986502 2315 84-85 0.8113977872451663 2315 86-87 0.8439086534625595 2315 88-89 0.9862305842997294 2315 90-91 1.036201362834472 2315 92-93 1.0678131471193026 2315 94-95 1.1489482613964022 2315 96-97 1.1857218024434708 2315 98-99 1.1126185177942993 2315 100 1.0941196256168304 2316 1 0.45415038518954987 2316 2 0.4429859996542902 2316 3 0.44185086110947935 2316 4 0.2663624929978212 2316 5 0.3654864964887423 2316 6 0.3857211102554956 2316 7 0.4810395995695771 2316 8 0.48202496403177975 2316 9 0.5383794567640905 2316 10-11 0.46468011312877167 2316 12-13 0.6073219664424201 2316 14-15 0.7569716079620079 2316 16-17 0.7184137059151752 2316 18-19 0.7943639620175205 2316 20-21 0.8213876090125751 2316 22-23 0.8429888156147669 2316 24-25 0.8887488305667546 2316 26-27 0.956178345975772 2316 28-29 0.8776434878834962 2316 30-31 0.7743167685159662 2316 32-33 0.7276821073785911 2316 34-35 0.656786162618431 2316 36-37 0.6411540647924667 2316 38-39 0.5695779765070839 2316 40-41 0.6533636981253252 2316 42-43 0.7510271502827237 2316 44-45 0.6956065214375471 2316 46-47 0.6873448010634675 2316 48-49 0.4741347031766665 2316 50-51 0.5966026141308092 2316 52-53 0.7273623762521098 2316 54-55 0.8136250155112705 2316 56-57 0.8403219909086133 2316 58-59 0.7730563822373 2316 60-61 0.8660386340472641 2316 62-63 0.8433244154095263 2316 64-65 0.8255609155759842 2316 66-67 0.828337144147028 2316 68-69 0.7730049624396145 2316 70-71 0.7130453656078473 2316 72-73 0.7330520167046828 2316 74-75 0.7738567737133266 2316 76-77 0.6181429742134554 2316 78-79 0.5849111020316897 2316 80-81 0.6210319908052 2316 82-83 0.5161151904181551 2316 84-85 0.6063670610318788 2316 86-87 0.5899699240822862 2316 88-89 0.5463117389537722 2316 90-91 0.7079389514234791 2316 92-93 0.6818603177463558 2316 94-95 0.6233228042039372 2316 96-97 0.7419674046595865 2316 98-99 0.8345814931723119 2316 100 0.4293830249021049 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 9.0 12 39.0 13 283.0 14 1066.0 15 3249.0 16 7621.0 17 15674.0 18 25746.0 19 35930.0 20 48163.0 21 64656.0 22 77934.0 23 81163.0 24 81458.0 25 88084.0 26 102052.0 27 124092.0 28 151002.0 29 183513.0 30 223890.0 31 276306.0 32 349175.0 33 455014.0 34 649671.0 35 858214.0 36 1279366.0 37 1998135.0 38 1793569.0 39 277756.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88241137609175 20.10020007359352 13.462239853236834 23.555148697077897 2 15.805082515577077 20.869452349808853 41.103792892553535 22.221672242060535 3 17.473788371788654 28.469546547721087 31.288783283091202 22.767881797399053 4 12.031057765706098 17.71911210036615 38.662804334822184 31.58702579910556 5 12.084204856145101 38.771797797412866 34.04585558276627 15.098141763675763 6 28.164754266762305 37.821909963180026 20.237290982171025 13.77604478788664 7 26.33109913689221 33.83756039495757 23.37219202489354 16.459148443256677 8 26.412379615131552 35.52855863247804 20.839075724341022 17.219986028049384 9 28.707736672418875 15.351272005397792 20.61497255982136 35.32601876236197 10-11 24.83572653105888 26.13035260995673 29.385404128215413 19.64851673076897 12-13 24.998395087346157 24.500499306158975 28.58186783733427 21.919237769160603 14-15 23.13575147654395 25.853133794243227 26.70128264167489 24.309832087537934 16-17 21.74157151580324 27.81893146435963 27.695553261967937 22.743943757869197 18-19 21.43712554141374 27.452536561942637 29.44250528404248 21.667832612601142 20-21 22.731389883146363 27.115992390242276 28.597605042420955 21.55501268419041 22-23 22.325964331673052 26.77826928952446 28.701572130300484 22.194194248502004 24-25 21.695333595270604 26.496167154525285 29.21395096419736 22.594548286006752 26-27 21.765958341299342 26.754114972272884 28.94761585529745 22.53231083113032 28-29 21.44467044855647 28.015856637271725 28.855733813664575 21.68373910050724 30-31 23.330073953429682 26.721259253635328 28.985243087687408 20.963423705247582 32-33 23.211323364995316 26.667958705837215 28.596727101924973 21.523990827242493 34-35 22.878035099204485 27.150679051145023 28.94006923069481 21.031216618955682 36-37 22.255957678300074 27.035230173000564 28.88346397241401 21.825348176285353 38-39 22.142055290806685 27.382688504530094 28.82993609814391 21.645320106519307 40-41 22.21045767117656 26.37505830639747 29.531682367064654 21.882801655361312 42-43 21.914968327681482 27.004500407826153 28.499315390279417 22.581215874212948 44-45 20.89591762233609 27.449603586675238 29.387271810028565 22.267206980960108 46-47 21.053191939544476 26.39377687755737 28.93272665376542 23.62030452913273 48-49 21.632049025184042 26.623204010856654 28.976385824739832 22.768361139219472 50-51 21.479268854594853 26.93303864947807 28.826747747960997 22.76094474796608 52-53 21.074001779073143 27.432602026072356 28.32123063133965 23.172165563514852 54-55 20.99948475877102 26.924585956061065 28.988058444157396 23.08787084101052 56-57 21.57681676187578 26.749364720204532 28.25407430720134 23.419744210718353 58-59 20.77155665080536 26.945858091293033 28.982022951608155 23.300562306293454 60-61 21.893742674096643 27.275994132954835 28.27068233121397 22.559580861734553 62-63 21.774965025374268 27.113933710405615 28.033824863112756 23.077276401107362 64-65 21.105663599000078 26.581144714031197 27.996747202553117 24.316444484415605 66-67 20.97316395924668 26.48415418394699 27.829972994097886 24.712708862708446 68-69 20.440349675008733 26.87708962154946 28.22387128942308 24.45868941401873 70-71 20.985613203862204 26.43592374523913 28.449727037911398 24.128736012987265 72-73 20.987159578824453 27.121969810616964 27.46901932482711 24.42185128573147 74-75 21.40031268398183 27.12905965405848 27.158404857650087 24.3122228043096 76-77 21.05367678365556 26.86153710939432 27.48235712801973 24.602428978930387 78-79 20.81730662182322 26.89538899809355 27.706090377358123 24.58121400272511 80-81 21.001933872863706 27.245067954121165 27.100104494088818 24.652893678926308 82-83 21.63496305697299 26.928111428785318 27.28384228020595 24.153083234035744 84-85 21.629130232305176 27.38957348779762 26.509450578965343 24.47184570093186 86-87 20.937770367259127 27.572323064421145 26.922644208621467 24.567262359698265 88-89 21.048007791184826 26.839462749100445 27.047586698667104 25.06494276104762 90-91 21.910455190269438 26.350456468154317 27.075607485875565 24.66348085570069 92-93 21.650974628788127 26.851308234360616 26.67978135105933 24.817935785791928 94-95 21.04015971562206 26.47352474053588 26.84151311345649 25.64480243038557 96-97 20.97090759510006 26.382185908743978 27.097634395307306 25.549272100848658 98-99 20.9201493891784 26.599804966890744 26.943588386525313 25.536457257405544 100 20.77785006977036 26.77859830810794 26.957626383043763 25.485925239077933 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1601.0 1 1164.0 2 1739.0 3 2621.5 4 1895.0 5 1508.5 6 2074.5 7 3676.5 8 5687.0 9 6718.5 10 6412.5 11 6003.5 12 6568.0 13 7417.5 14 8461.0 15 9673.0 16 10864.0 17 12027.0 18 13208.0 19 14297.5 20 15596.5 21 17186.0 22 19088.0 23 22234.5 24 25986.5 25 30799.5 26 38833.5 27 48380.5 28 58345.5 29 73349.5 30 94783.5 31 114636.0 32 140858.5 33 170363.0 34 196488.0 35 221130.0 36 245753.5 37 277215.0 38 288585.5 39 280858.5 40 272416.0 41 264575.0 42 263304.0 43 270618.5 44 285939.0 45 313343.0 46 336884.0 47 352823.5 48 376234.0 49 398921.5 50 417735.5 51 428109.0 52 417688.5 53 392203.0 54 360982.5 55 315291.0 56 272683.0 57 234608.0 58 193837.0 59 156066.5 60 115585.5 61 82926.5 62 62386.0 63 47593.5 64 34120.5 65 22863.5 66 15917.5 67 11549.0 68 8642.0 69 6455.5 70 4842.0 71 3556.5 72 2652.5 73 1983.0 74 1448.0 75 995.5 76 669.0 77 406.0 78 255.5 79 161.5 80 98.0 81 67.0 82 47.0 83 29.0 84 16.5 85 11.5 86 9.5 87 6.5 88 4.0 89 3.0 90 1.5 91 1.5 92 1.0 93 1.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051465174169410766 2 0.0 3 0.0 4 0.015703254529221725 5 0.00406360888023907 6 0.0 7 1.8372699724485158E-4 8 3.7826146491587085E-4 9 0.0020101894992671995 10-11 7.727341354709932E-4 12-13 2.0534193809718706E-4 14-15 2.1614940852335477E-5 16-17 9.726723383550966E-5 18-19 0.0 20-21 2.1614940852335477E-5 22-23 0.0 24-25 8.105602819625805E-5 26-27 2.1614940852335477E-5 28-29 8.375789580279997E-4 30-31 8.26771487601832E-4 32-33 0.01441176181329468 34-35 0.023706186379798936 36-37 0.03184961534591633 38-39 0.02111779721273176 40-41 1.621120563925161E-4 42-43 0.0015346608005158188 44-45 0.009040449011489314 46-47 0.014022692877952643 48-49 0.005095722305938089 50-51 0.009834798087812643 52-53 0.015265551976961932 54-55 0.009532188915879947 56-57 0.0026262153135587604 58-59 0.008473056814115507 60-61 0.0017345990033999221 62-63 8.591938988803352E-4 64-65 0.018859035893662703 66-67 0.04474292756433444 68-69 0.054815490001522776 70-71 0.047698770725891314 72-73 0.04049018795163743 74-75 0.028369609868690315 76-77 0.02802917455026603 78-79 0.012071944466029366 80-81 0.0019723633527756125 82-83 0.02665662580614273 84-85 0.04731510552576236 86-87 0.04802299483867635 88-89 0.059278975287530046 90-91 0.03457850162852368 92-93 0.026089233608768924 94-95 0.007181564098188462 96-97 0.0036853474153231993 98-99 0.022933452244327943 100 0.01941021688539726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9252859.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.49637990779128 #Duplication Level Percentage of deduplicated Percentage of total 1 76.40747790341109 43.167458994272806 2 14.245983368106845 16.096929770492807 3 4.481030328882505 7.594859755166426 4 1.7748950647434074 4.0110058349682935 5 0.8952021639536175 2.5287840774500214 6 0.5634704613189833 1.9100424749697409 7 0.3415580015241494 1.3507753430258007 8 0.21670128320355092 0.9794270417898947 9 0.16591964532111123 0.8436473384604735 >10 0.806964685310922 8.026061899019307 >50 0.04984782135750985 1.9393538511278012 >100 0.04282871258883549 5.097733590787066 >500 0.00517695334604333 2.0147732450522464 >1k 0.0027152236363611405 2.7182964262619222 >5k 1.522555300196647E-4 0.6321669582428439 >10k+ 7.612776500983235E-5 1.0886833989124636 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32113 0.3470602977955246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25843 0.2792974582234529 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17982 0.1943399332033483 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0807470426167738E-5 2 0.0 1.0807470426167738E-5 0.0 0.0 1.0807470426167738E-5 3 0.0 1.0807470426167738E-5 0.0 0.0 1.0807470426167738E-5 4 0.0 1.0807470426167738E-5 0.0 3.242241127850322E-5 1.0807470426167738E-5 5 0.0 1.0807470426167738E-5 0.0 4.322988170467095E-5 1.0807470426167738E-5 6 0.0 1.0807470426167738E-5 0.0 4.322988170467095E-5 1.0807470426167738E-5 7 0.0 1.0807470426167738E-5 0.0 5.403735213083869E-5 1.0807470426167738E-5 8 0.0 1.0807470426167738E-5 0.0 5.403735213083869E-5 1.0807470426167738E-5 9 0.0 1.0807470426167738E-5 0.0 5.403735213083869E-5 1.0807470426167738E-5 10-11 0.0 1.0807470426167738E-5 0.0 1.080747042616774E-4 1.0807470426167738E-5 12-13 0.0 1.0807470426167738E-5 0.0 1.6211205639251607E-4 1.0807470426167738E-5 14-15 0.0 2.7018676065419347E-5 0.0 1.9993820288410316E-4 1.0807470426167738E-5 16-17 0.0 4.322988170467095E-5 0.0 2.48571819801858E-4 1.0807470426167738E-5 18-19 0.0 4.322988170467095E-5 0.0 3.458390536373677E-4 1.0807470426167738E-5 20-21 0.0 4.322988170467095E-5 0.0 5.890071382261418E-4 1.0807470426167738E-5 22-23 0.0 4.322988170467095E-5 0.0 0.0017345990033999221 2.7018676065419347E-5 24-25 0.0 4.322988170467095E-5 0.0 0.004339199376106348 4.322988170467095E-5 26-27 0.0 4.322988170467095E-5 0.0 0.007911068351954785 4.322988170467095E-5 28-29 0.0 4.322988170467095E-5 0.0 0.020631461043554213 5.403735213083869E-5 30-31 0.0 4.322988170467095E-5 0.0 0.06899489120065486 5.403735213083869E-5 32-33 0.0 4.322988170467095E-5 0.0 0.17942022028002375 5.403735213083869E-5 34-35 0.0 4.322988170467095E-5 0.0 0.32187348796734067 5.403735213083869E-5 36-37 0.0 4.863361691775482E-5 0.0 0.5400979308125197 5.403735213083869E-5 38-39 0.0 5.944108734392257E-5 0.0 0.9300098488477994 6.484482255700644E-5 40-41 0.0 7.024855777009031E-5 0.0 1.4399441297008848 7.565229298317418E-5 42-43 0.0 7.565229298317418E-5 0.0 1.902503863940864 8.105602819625805E-5 44-45 0.0 7.565229298317418E-5 0.0 2.3920984854519016 9.726723383550965E-5 46-47 0.0 7.565229298317418E-5 0.0 2.9251769642226257 9.726723383550965E-5 48-49 0.0 7.565229298317418E-5 0.0 3.50640812747714 9.726723383550965E-5 50-51 0.0 7.565229298317418E-5 0.0 4.150657650786638 9.726723383550965E-5 52-53 0.0 7.565229298317418E-5 0.0 4.817975719720791 1.1347843947476127E-4 54-55 5.403735213083869E-6 9.726723383550965E-5 0.0 5.523876458076363 1.2968964511401288E-4 56-57 1.0807470426167738E-5 9.726723383550965E-5 0.0 6.219137241797373 1.2968964511401288E-4 58-59 1.0807470426167738E-5 9.726723383550965E-5 0.0 7.048670038093091 1.4049711554018062E-4 60-61 1.0807470426167738E-5 9.726723383550965E-5 0.0 7.84812024045757 1.5130458596634836E-4 62-63 1.0807470426167738E-5 9.726723383550965E-5 0.0 8.691978338803175 1.6211205639251607E-4 64-65 1.0807470426167738E-5 9.726723383550965E-5 0.0 9.449068660832289 1.6211205639251607E-4 66-67 1.0807470426167738E-5 9.726723383550965E-5 0.0 10.269744735113763 1.6751579160559994E-4 68-69 1.0807470426167738E-5 9.726723383550965E-5 0.0 11.23395482412517 1.729195268186838E-4 70-71 1.0807470426167738E-5 1.0267096904859352E-4 0.0 12.21015580157441 1.8372699724485155E-4 72-73 1.0807470426167738E-5 1.0807470426167739E-4 0.0 13.067263858662496 2.1614940852335477E-4 74-75 1.0807470426167738E-5 1.1347843947476126E-4 0.0 13.882638868699933 2.1614940852335477E-4 76-77 1.0807470426167738E-5 1.1888217468784513E-4 0.0 14.761361866640353 2.1614940852335477E-4 78-79 1.0807470426167738E-5 1.1888217468784513E-4 0.0 15.690561155206192 2.1614940852335477E-4 80-81 1.0807470426167738E-5 1.1888217468784513E-4 0.0 16.576579195684275 2.4316808458877412E-4 82-83 1.0807470426167738E-5 1.1888217468784513E-4 0.0 17.478138378635187 2.701867606541935E-4 84-85 1.0807470426167738E-5 1.24285909900929E-4 0.0 18.376147307551104 2.701867606541935E-4 86-87 1.0807470426167738E-5 1.2968964511401288E-4 0.0 19.237545930398376 2.701867606541935E-4 88 1.0807470426167738E-5 1.2968964511401288E-4 0.0 19.902745735129002 2.701867606541935E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 9025 0.0 56.445374 2 GGTATCA 16115 0.0 53.190044 1 ACCTGGG 9975 0.0 49.987392 3 GTATCAA 29760 0.0 42.02645 1 CCTGGGG 8745 0.0 39.49959 4 CTGGGGG 6830 0.0 35.63721 5 TAACGCA 1060 0.0 35.469017 4 ATCAACG 37580 0.0 32.57684 3 CAACGCA 37985 0.0 32.237167 5 TGGGGGG 27035 0.0 32.065903 6 GTACATG 42220 0.0 31.772951 1 TATCAAC 39170 0.0 31.601557 2 TACATGG 42905 0.0 30.854975 2 TCAACGC 39915 0.0 30.730484 4 AACGCAG 40690 0.0 30.542984 6 GTACCTG 16965 0.0 30.23743 1 ACATGGG 42380 0.0 29.885176 3 ATGGGGG 25750 0.0 29.634895 5 CATGGGG 31690 0.0 29.348682 4 ATAACGC 1400 0.0 28.533094 3 >>END_MODULE