FastQCFastQC Report
Mon 6 Jun 2016
ERR1211434_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211434_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8763738
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327000.3731284527218865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239910.27375304921256205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170950.1950651651156162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG106750.060.2798422
ACCTGGG119450.053.0117723
GGTATCA142050.052.9115561
TAACGCA11800.044.221084
GTATCAA269600.043.5571251
CCTGGGG109150.042.379884
ATAACGC13350.039.4391563
GTACCTG173300.037.9320871
GTACATG347450.035.113231
CTGGGGG81000.035.054425
TACATGG347050.034.5875362
ATCAACG335250.034.481063
CAACGCA340250.033.9881745
TATCAAC345400.033.5670782
TGGGGGG258150.033.4894456
ATGGGGG228800.033.326195
TCAACGC350950.032.9785234
ACATGGG355750.032.903743
CATGGGG285900.032.573074
AACGCAG361400.032.4939546
Produced by FastQC (version 0.11.5)