FastQCFastQC Report
Mon 6 Jun 2016
ERR1211434_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211434_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8763738
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292580.3338529746096928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227450.2595353717785721No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170110.19410667000770676No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT112540.12841552314777097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG109600.060.759182
ACCTGGG122150.053.5172353
GGTATCA139500.052.1436841
CCTGGGG106950.045.1291474
GTATCAA253100.041.372681
CTGGGGG76700.039.0861935
TAACGCA12500.038.725294
GTACCTG185000.036.5233541
TGGGGGG224300.034.0413936
ATAACGC13100.033.722253
GTACATG328000.032.6418531
ATCAACG318700.032.2202843
CAACGCA325350.031.7160875
TACATGG333750.031.4849572
TATCAAC331650.031.2592182
TCAACGC333250.030.9832784
ATGGGGG204800.030.6760545
ACATGGG331900.030.613183
CATGGGG263400.030.278444
AACGCAG349300.030.1592276
Produced by FastQC (version 0.11.5)