##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211434_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8763738 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07439200030854 33.0 31.0 34.0 30.0 34.0 2 32.19656509585293 33.0 31.0 34.0 30.0 34.0 3 32.275502645104176 34.0 31.0 34.0 30.0 34.0 4 35.92737083194409 37.0 35.0 37.0 35.0 37.0 5 35.796628676028426 37.0 35.0 37.0 35.0 37.0 6 35.83220961192587 37.0 35.0 37.0 35.0 37.0 7 35.763567212986054 37.0 35.0 37.0 33.0 37.0 8 35.741390260639925 37.0 35.0 37.0 33.0 37.0 9 37.329201762992 39.0 37.0 39.0 34.0 39.0 10-11 37.29657846914182 39.0 37.0 39.0 34.0 39.0 12-13 37.19926149093001 39.0 37.0 39.0 33.0 39.0 14-15 38.39427485166718 40.0 38.0 41.0 33.5 41.0 16-17 38.38353605504866 40.0 38.0 41.0 33.5 41.0 18-19 38.39825574429541 40.0 38.0 41.0 33.5 41.0 20-21 38.36215100223215 40.0 38.0 41.0 34.0 41.0 22-23 38.2595511755372 40.0 38.0 41.0 34.0 41.0 24-25 38.14362181982163 40.0 37.0 41.0 33.0 41.0 26-27 38.01873047779384 40.0 37.0 41.0 33.0 41.0 28-29 37.9326405011195 40.0 37.0 41.0 33.0 41.0 30-31 37.966894149505606 40.0 37.0 41.0 33.0 41.0 32-33 37.788534013682295 40.0 37.0 41.0 33.0 41.0 34-35 37.675350118864806 40.0 37.0 41.0 33.0 41.0 36-37 37.545656431079976 40.0 36.5 41.0 32.5 41.0 38-39 37.42534024864732 40.0 36.0 41.0 32.0 41.0 40-41 37.35984045848929 40.0 36.0 41.0 32.0 41.0 42-43 37.233764348044176 40.0 36.0 41.0 31.0 41.0 44-45 37.095383043171765 39.5 36.0 41.0 31.0 41.0 46-47 36.88197051303907 39.0 36.0 41.0 30.5 41.0 48-49 36.676049192707495 39.0 35.0 41.0 30.0 41.0 50-51 36.50256517253254 39.0 35.0 41.0 30.0 41.0 52-53 36.31009844201185 39.0 35.0 40.0 29.5 41.0 54-55 36.11854057024526 39.0 35.0 40.0 29.0 41.0 56-57 35.903198441121816 38.0 35.0 40.0 28.0 41.0 58-59 35.65562982371221 38.0 34.5 40.0 28.0 41.0 60-61 35.39009398729172 38.0 34.0 40.0 27.5 41.0 62-63 35.080173437407645 37.0 34.0 40.0 27.0 41.0 64-65 34.732659967698716 37.0 34.0 39.5 26.0 41.0 66-67 34.38728479787963 36.0 33.0 39.0 26.0 41.0 68-69 34.03241601928309 36.0 33.0 39.0 26.0 40.0 70-71 33.65722389236191 35.0 33.0 38.5 25.5 40.0 72-73 33.29631636637243 35.0 32.0 37.5 25.0 40.0 74-75 32.924897743405836 35.0 32.0 37.0 25.0 39.0 76-77 31.916272941979784 34.0 30.5 36.0 23.5 38.5 78-79 32.40772579006812 35.0 32.0 36.0 25.0 38.5 80-81 32.317346947158846 35.0 32.0 36.0 25.0 37.5 82-83 32.04210435090597 34.5 31.5 35.0 24.5 37.0 84-85 31.76110205485376 34.0 31.0 35.0 24.0 37.0 86-87 31.515167443390023 34.0 31.0 35.0 23.0 36.0 88-89 31.30821362984608 34.0 31.0 35.0 23.0 36.0 90-91 31.09881822117457 34.0 31.0 35.0 22.0 35.5 92-93 30.916680587667045 34.0 31.0 35.0 20.0 35.0 94-95 30.74892129363064 34.0 30.0 35.0 20.0 35.0 96-97 30.591187059677047 34.0 30.0 35.0 20.0 35.0 98-99 30.41536533839784 34.0 30.0 35.0 19.0 35.0 100 28.62731690518361 32.0 26.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2797869724787212 1101 2 -0.27734983352985765 1101 3 -0.2911387258617033 1101 4 -0.1931006410530287 1101 5 -0.1849295211277706 1101 6 -0.21108419854981975 1101 7 -0.2658442322282468 1101 8 -0.3145012021208018 1101 9 -0.4777471241611124 1101 10-11 -0.40048915969956767 1101 12-13 -0.4492731209579901 1101 14-15 -0.5864966688756468 1101 16-17 -0.6452067546359572 1101 18-19 -0.6228803228358331 1101 20-21 -0.6693968082527633 1101 22-23 -0.6921893519945641 1101 24-25 -0.7321658003814662 1101 26-27 -0.7094717551800471 1101 28-29 -0.760447877406115 1101 30-31 -0.6428281958629682 1101 32-33 -0.5729157342923514 1101 34-35 -0.6421124461363377 1101 36-37 -0.6437791579285062 1101 38-39 -0.6936970048305469 1101 40-41 -0.6978353289011281 1101 42-43 -0.7040862625591515 1101 44-45 -0.7552636643589992 1101 46-47 -0.7672248753235351 1101 48-49 -0.7337453250375106 1101 50-51 -0.7856491810186057 1101 52-53 -0.7934053808270463 1101 54-55 -0.7937626779802045 1101 56-57 -0.748554833759556 1101 58-59 -0.7528848915432391 1101 60-61 -0.7727062419648902 1101 62-63 -0.7865482695297885 1101 64-65 -0.8493160102361657 1101 66-67 -0.84611535161077 1101 68-69 -0.9142151899219542 1101 70-71 -0.9872683032794285 1101 72-73 -1.0833525782286628 1101 74-75 -1.088755486648104 1101 76-77 -0.9057160613432025 1101 78-79 -0.8642352366863406 1101 80-81 -0.7331276721334206 1101 82-83 -0.8102427452091199 1101 84-85 -0.8329334469568472 1101 86-87 -0.9317651140942118 1101 88-89 -0.9413974768113711 1101 90-91 -1.0007800069272328 1101 92-93 -1.03525758147628 1101 94-95 -1.0488697247437777 1101 96-97 -1.0934933869566201 1101 98-99 -1.1957215958382967 1101 100 -1.121956579808291 1102 1 -0.260713685449776 1102 2 -0.210766339781852 1102 3 -0.24881812995209174 1102 4 -0.12135115632103322 1102 5 -0.1330093961024943 1102 6 -0.26851666983418454 1102 7 -0.2668221756000051 1102 8 -0.2962734000073439 1102 9 -0.4386396981451668 1102 10-11 -0.42319378442645217 1102 12-13 -0.4250070224505791 1102 14-15 -0.5378855352618999 1102 16-17 -0.6361170055258398 1102 18-19 -0.5897152843328186 1102 20-21 -0.6391474283248044 1102 22-23 -0.6748995523980668 1102 24-25 -0.7126848053028354 1102 26-27 -0.6957569360920246 1102 28-29 -0.7672607754128862 1102 30-31 -0.6755917052753446 1102 32-33 -0.6034113671819981 1102 34-35 -0.7152323493290424 1102 36-37 -0.6604339225540059 1102 38-39 -0.6227262595489691 1102 40-41 -0.6563020419751169 1102 42-43 -0.6781406467386759 1102 44-45 -0.6414605020408217 1102 46-47 -0.7023902334804646 1102 48-49 -0.7157447386558786 1102 50-51 -0.7181232530580886 1102 52-53 -0.7408523413732695 1102 54-55 -0.6905439454349747 1102 56-57 -0.6936024918006538 1102 58-59 -0.8715240582523194 1102 60-61 -0.7681573447327636 1102 62-63 -0.7730914815477519 1102 64-65 -0.7884839598707529 1102 66-67 -0.7711252808326137 1102 68-69 -0.8573306604660473 1102 70-71 -0.7542292678883129 1102 72-73 -0.8945860067846851 1102 74-75 -0.9696259186739269 1102 76-77 -0.7213563822627691 1102 78-79 -0.5625588866854478 1102 80-81 -0.5453658804398884 1102 82-83 -0.5401104735512021 1102 84-85 -0.6358475822358329 1102 86-87 -0.6555245125450675 1102 88-89 -0.6393577833690678 1102 90-91 -0.6310345921119485 1102 92-93 -0.621876627906282 1102 94-95 -0.7435447709048901 1102 96-97 -0.8940264627496717 1102 98-99 -0.8341356314597235 1102 100 -0.8754519645229877 1103 1 -0.3819621102157953 1103 2 -0.32870274949682 1103 3 -0.3558593737844902 1103 4 -0.20238376125026747 1103 5 -0.1924893350409107 1103 6 -0.2930437714326857 1103 7 -0.320531831905825 1103 8 -0.30690767318558443 1103 9 -0.44483740678327877 1103 10-11 -0.45663488314102807 1103 12-13 -0.5698616294427197 1103 14-15 -0.7121413961800513 1103 16-17 -0.773717078564438 1103 18-19 -0.7169368644482432 1103 20-21 -0.672667596432234 1103 22-23 -0.73302136287667 1103 24-25 -0.7514515140178233 1103 26-27 -0.8077318985975737 1103 28-29 -0.8411346259165313 1103 30-31 -0.7071414186689182 1103 32-33 -0.7384186710416145 1103 34-35 -0.7788441413636988 1103 36-37 -0.7063314565735652 1103 38-39 -0.6888600512102201 1103 40-41 -0.7997750884482926 1103 42-43 -0.7893343196976943 1103 44-45 -0.7382877980765059 1103 46-47 -0.8342591088767009 1103 48-49 -0.8328030536506716 1103 50-51 -0.7576350854747744 1103 52-53 -0.8758971996441787 1103 54-55 -0.8344731517720376 1103 56-57 -0.9428477112503231 1103 58-59 -0.984080482966327 1103 60-61 -1.0026753808857265 1103 62-63 -1.0295500513318672 1103 64-65 -0.968177983569042 1103 66-67 -0.9395170835440823 1103 68-69 -0.9646073609897527 1103 70-71 -0.959550895227629 1103 72-73 -0.9616612938487137 1103 74-75 -1.0536390184099886 1103 76-77 -0.9028980356591596 1103 78-79 -0.7417816025220105 1103 80-81 -0.7642382596942454 1103 82-83 -0.7540543254775045 1103 84-85 -0.7412258125937079 1103 86-87 -0.8439201773648151 1103 88-89 -0.8422622005126108 1103 90-91 -0.936386038916158 1103 92-93 -0.8484615471403139 1103 94-95 -0.908429212509926 1103 96-97 -1.0600998801720642 1103 98-99 -1.162831878631625 1103 100 -1.073211695565238 1104 1 -0.37481155183815673 1104 2 -0.3219653169803536 1104 3 -0.3627184234063776 1104 4 -0.2242495550370478 1104 5 -0.2026938478545759 1104 6 -0.299879769649138 1104 7 -0.29580471649106954 1104 8 -0.3650417974934044 1104 9 -0.47739694301009905 1104 10-11 -0.44861363005545485 1104 12-13 -0.5316945518632892 1104 14-15 -0.7247565182494995 1104 16-17 -0.6758042328569616 1104 18-19 -0.6996557873376688 1104 20-21 -0.6615580910254266 1104 22-23 -0.6843935544057373 1104 24-25 -0.7619386625025086 1104 26-27 -0.7326846819973909 1104 28-29 -0.7570591076508251 1104 30-31 -0.7384185149289806 1104 32-33 -0.6857591624516601 1104 34-35 -0.790724860547698 1104 36-37 -0.7065610088600494 1104 38-39 -0.8219084509711081 1104 40-41 -0.8769620525341395 1104 42-43 -0.7659084297490111 1104 44-45 -0.8402075551103465 1104 46-47 -0.8264137817595625 1104 48-49 -0.8914344895379216 1104 50-51 -0.8254165176948973 1104 52-53 -0.8470593950414198 1104 54-55 -0.8294920160742763 1104 56-57 -0.8877907789326045 1104 58-59 -0.9907324422365136 1104 60-61 -1.0242900601696405 1104 62-63 -1.069433558898254 1104 64-65 -1.0577696752344323 1104 66-67 -1.0726261057090554 1104 68-69 -0.9835260961917172 1104 70-71 -0.9853412450840509 1104 72-73 -0.98206551063641 1104 74-75 -0.8660320217282731 1104 76-77 -0.767454228780668 1104 78-79 -0.6972573785663414 1104 80-81 -0.817068311454733 1104 82-83 -0.8349901213255713 1104 84-85 -0.7371197771214746 1104 86-87 -0.6505907856253224 1104 88-89 -0.7641541109522834 1104 90-91 -0.7582907718940142 1104 92-93 -0.9444114245117987 1104 94-95 -0.9316824340912042 1104 96-97 -0.9481311064807763 1104 98-99 -0.9755915327029356 1104 100 -1.0485953385857734 1105 1 -0.3935438835506062 1105 2 -0.3735436259216378 1105 3 -0.4070040874764196 1105 4 -0.2527176756812466 1105 5 -0.2959354219999497 1105 6 -0.298458579491772 1105 7 -0.3852491220472203 1105 8 -0.38857129749204233 1105 9 -0.5448479763426093 1105 10-11 -0.5164119080214604 1105 12-13 -0.5600056446312536 1105 14-15 -0.7945847193894195 1105 16-17 -0.7177341909091268 1105 18-19 -0.7087763282020276 1105 20-21 -0.7743081061009534 1105 22-23 -0.7507444608008242 1105 24-25 -0.696495173901873 1105 26-27 -0.7787644244013663 1105 28-29 -0.7740110729386345 1105 30-31 -0.7484485720402176 1105 32-33 -0.6631646404276097 1105 34-35 -0.7030045495585568 1105 36-37 -0.8243415522516528 1105 38-39 -0.7335283168459057 1105 40-41 -0.7927605053459317 1105 42-43 -0.8406744333653222 1105 44-45 -0.9302964204036428 1105 46-47 -1.0041876404059948 1105 48-49 -0.8703292475280264 1105 50-51 -0.8514139340984528 1105 52-53 -0.837182926465637 1105 54-55 -0.8979200873374182 1105 56-57 -0.8654713511419416 1105 58-59 -0.908795481262274 1105 60-61 -0.9294614044912848 1105 62-63 -0.8573773134038589 1105 64-65 -0.9529150684419889 1105 66-67 -0.9107083656498816 1105 68-69 -0.9458518072186024 1105 70-71 -0.9631641257001391 1105 72-73 -1.0081232897434589 1105 74-75 -0.9951050345392858 1105 76-77 -0.8727968507583306 1105 78-79 -0.798630289166816 1105 80-81 -0.7118811719226485 1105 82-83 -0.7313855482447273 1105 84-85 -0.6605533392088461 1105 86-87 -0.6703083521114159 1105 88-89 -0.7593002617238902 1105 90-91 -0.7744247119452403 1105 92-93 -0.7955660581826116 1105 94-95 -0.7664277607543362 1105 96-97 -0.9922925603492168 1105 98-99 -1.078508025508654 1105 100 -1.0780474894767735 1106 1 -0.3680879085599962 1106 2 -0.331112016013698 1106 3 -0.36695610684060753 1106 4 -0.23199860194571897 1106 5 -0.2563595912378034 1106 6 -0.32144728595335437 1106 7 -0.38718401864662155 1106 8 -0.38759955081511066 1106 9 -0.5049898854758155 1106 10-11 -0.4799572364242053 1106 12-13 -0.5456211700116 1106 14-15 -0.6744336582947099 1106 16-17 -0.6880575900658314 1106 18-19 -0.6741563696724171 1106 20-21 -0.7011864185938776 1106 22-23 -0.736276167825217 1106 24-25 -0.8029353519084239 1106 26-27 -0.7352185049717619 1106 28-29 -0.7683322449986392 1106 30-31 -0.7809602213141247 1106 32-33 -0.6748577712178303 1106 34-35 -0.6631139298265936 1106 36-37 -0.701644016072521 1106 38-39 -0.7605584829468484 1106 40-41 -0.7619212830119224 1106 42-43 -0.7267596012326223 1106 44-45 -0.7858307154093254 1106 46-47 -0.7741138426419596 1106 48-49 -0.8319619771632247 1106 50-51 -0.7905706059187025 1106 52-53 -0.762905781429879 1106 54-55 -0.8693152823785297 1106 56-57 -0.9468053793795903 1106 58-59 -0.9084223507765259 1106 60-61 -0.971129985279056 1106 62-63 -0.9089895406751793 1106 64-65 -0.9911622528929698 1106 66-67 -0.9649673410868971 1106 68-69 -0.9181125879740506 1106 70-71 -0.8836816458409444 1106 72-73 -0.9527339978070799 1106 74-75 -0.8996434940456624 1106 76-77 -0.8745834085685935 1106 78-79 -0.8111414100985286 1106 80-81 -0.7015093443825009 1106 82-83 -0.6829841416007802 1106 84-85 -0.6972287503866887 1106 86-87 -0.7416606977045284 1106 88-89 -0.8129013391837852 1106 90-91 -0.8192705770630617 1106 92-93 -0.8337083577517426 1106 94-95 -0.8069982180971991 1106 96-97 -0.8574879283104551 1106 98-99 -0.8617643462499807 1106 100 -0.9752195154586758 1107 1 -0.3531907138076278 1107 2 -0.2909904804557186 1107 3 -0.3969171939718059 1107 4 -0.20854781695738467 1107 5 -0.24473087000095006 1107 6 -0.2746957641801089 1107 7 -0.3116713885773663 1107 8 -0.376654269507128 1107 9 -0.4985298076396134 1107 10-11 -0.45761551221750807 1107 12-13 -0.5041890372004545 1107 14-15 -0.6616809121350897 1107 16-17 -0.6547585769175299 1107 18-19 -0.6621113474823446 1107 20-21 -0.6304169636195667 1107 22-23 -0.6966597228791827 1107 24-25 -0.7033391570774015 1107 26-27 -0.705328998736988 1107 28-29 -0.7489788161894424 1107 30-31 -0.761472020800916 1107 32-33 -0.7090413975592185 1107 34-35 -0.6915203371289493 1107 36-37 -0.7020162163943127 1107 38-39 -0.6776098079459985 1107 40-41 -0.7731612344095495 1107 42-43 -0.7315706016604224 1107 44-45 -0.754361860305309 1107 46-47 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0.8018964513813103 2311 28-29 0.7082796528581881 2311 30-31 0.7871517773977388 2311 32-33 0.9534047877112073 2311 34-35 0.9467802586851377 2311 36-37 0.9085972087037391 2311 38-39 0.9786430532816652 2311 40-41 0.9280802387846663 2311 42-43 1.0422382800916026 2311 44-45 0.9997464916125409 2311 46-47 0.9910379809788381 2311 48-49 0.981665750331878 2311 50-51 1.0045061001723568 2311 52-53 1.0308673279264369 2311 54-55 0.9868109704947088 2311 56-57 1.0301550743413443 2311 58-59 1.0329470608533171 2311 60-61 0.988085237598348 2311 62-63 1.0384123212041274 2311 64-65 1.0406762265368599 2311 66-67 1.0608407386374736 2311 68-69 1.082704590429067 2311 70-71 1.075543817550873 2311 72-73 1.0983106701450325 2311 74-75 0.9848388896941671 2311 76-77 0.9231681284300919 2311 78-79 0.8532584961232672 2311 80-81 0.877958469387778 2311 82-83 0.878906946000189 2311 84-85 0.9123721432013276 2311 86-87 0.9750553541175044 2311 88-89 0.9878822322677472 2311 90-91 0.9955901864828753 2311 92-93 1.029925699577415 2311 94-95 1.0775777705353669 2311 96-97 1.1144739948449711 2311 98-99 1.1661333720779687 2311 100 1.3739168356846818 2312 1 0.09598374493030803 2312 2 0.11104534914426267 2312 3 0.316702096298755 2312 4 0.23884435417437544 2312 5 0.29478173773338767 2312 6 0.350806324041109 2312 7 0.4229499848455802 2312 8 0.277970853480511 2312 9 0.3582374374125905 2312 10-11 0.2805719376592961 2312 12-13 0.4230880162216337 2312 14-15 0.7589035496066998 2312 16-17 0.8457042506990362 2312 18-19 0.8658587623542005 2312 20-21 0.8988285564703062 2312 22-23 0.5190233292162532 2312 24-25 0.48942350492996667 2312 26-27 0.7689315250914959 2312 28-29 0.8872546771180154 2312 30-31 0.7805219621774953 2312 32-33 0.6995764569993241 2312 34-35 0.8336144640829275 2312 36-37 0.8816848982735053 2312 38-39 0.8553378022190969 2312 40-41 0.9036344855977418 2312 42-43 0.7843063914288919 2312 44-45 0.718972511681649 2312 46-47 0.9224235166786983 2312 48-49 0.9724055660608286 2312 50-51 0.9540563650910414 2312 52-53 0.9559502212376358 2312 54-55 0.9741723181552047 2312 56-57 0.997734138103759 2312 58-59 0.9727905686171923 2312 60-61 0.9742451803799312 2312 62-63 0.9756400336483608 2312 64-65 1.0633735300158307 2312 66-67 1.044189931873312 2312 68-69 1.0522757427319291 2312 70-71 0.9395249594208863 2312 72-73 1.0274978215599901 2312 74-75 1.0923521291064944 2312 76-77 1.0301233219803692 2312 78-79 0.8850418999591199 2312 80-81 0.7268169793764088 2312 82-83 0.7218532495648624 2312 84-85 0.6034458628211219 2312 86-87 0.7955197423445917 2312 88-89 0.45790427826116087 2312 90-91 0.819557070553742 2312 92-93 0.93025960155002 2312 94-95 1.0289510850178978 2312 96-97 1.1264906620142128 2312 98-99 1.0896786192595762 2312 100 1.2031202066697482 2313 1 -0.3734713177615916 2313 2 -0.02609971381309606 2313 3 0.23900461901737913 2313 4 0.1454918195102195 2313 5 0.2558233878090448 2313 6 0.3787865121610636 2313 7 0.14689258436952457 2313 8 0.11047242409627955 2313 9 0.26595756695520834 2313 10-11 0.416161095543778 2313 12-13 0.606676519314199 2313 14-15 0.6655250223576203 2313 16-17 0.7940610769048675 2313 18-19 0.6807764045723488 2313 20-21 0.8627224226626993 2313 22-23 0.9144314902242741 2313 24-25 0.7815177772703166 2313 26-27 0.975321166106248 2313 28-29 0.8608871353307777 2313 30-31 0.8479973853589016 2313 32-33 1.0198566460041292 2313 34-35 1.0338627762240122 2313 36-37 0.8984014485818719 2313 38-39 0.6948102522912194 2313 40-41 0.8283959068501687 2313 42-43 0.9038050331780667 2313 44-45 1.0777372113179737 2313 46-47 1.0780510772248348 2313 48-49 1.0874381286863724 2313 50-51 1.0265721376127956 2313 52-53 0.9982419266166644 2313 54-55 1.072064485427994 2313 56-57 1.100977127848303 2313 58-59 1.037527811235293 2313 60-61 1.0602814107251177 2313 62-63 1.1248674455390315 2313 64-65 1.1985272291477855 2313 66-67 1.1841722790369147 2313 68-69 1.2534537045593268 2313 70-71 1.125627664419433 2313 72-73 1.224424060134318 2313 74-75 0.8450216228815464 2313 76-77 1.0041396962027989 2313 78-79 0.8291182451583481 2313 80-81 0.8830512179153374 2313 82-83 0.9359318290203298 2313 84-85 1.0134747975454452 2313 86-87 1.1026589652658494 2313 88-89 1.0701336235577195 2313 90-91 1.111240217625319 2313 92-93 0.8716875788623639 2313 94-95 0.9198253649679913 2313 96-97 1.1408610220612871 2313 98-99 1.2314829913229666 2313 100 1.37878672291124 2314 1 -0.3362584593178717 2314 2 -0.14052635739109576 2314 3 0.12163406050628112 2314 4 -0.17287574471199463 2314 5 0.15127893024105532 2314 6 0.15695370102790918 2314 7 0.1133371651672519 2314 8 0.1835562766139347 2314 9 0.2709012965501927 2314 10-11 0.42480817851566854 2314 12-13 0.5147530798556019 2314 14-15 0.5759294131161568 2314 16-17 0.7417288599868996 2314 18-19 0.7979032087401521 2314 20-21 0.47323328230618245 2314 22-23 0.6884727036442158 2314 24-25 0.8456679673058503 2314 26-27 0.8298363744324959 2314 28-29 0.7802601450937559 2314 30-31 0.762917615031931 2314 32-33 0.8151437939576738 2314 34-35 0.8217919235115119 2314 36-37 0.7978543355976413 2314 38-39 0.8081556426545831 2314 40-41 0.8885771242035148 2314 42-43 0.6747378029665683 2314 44-45 0.7139193367183978 2314 46-47 0.838025566731659 2314 48-49 0.751403784839944 2314 50-51 0.7797503891908164 2314 52-53 0.9353618966701163 2314 54-55 0.9534125030596101 2314 56-57 0.8352759923142301 2314 58-59 0.8988835311824559 2314 60-61 0.9419703572625693 2314 62-63 0.5974808289611815 2314 64-65 0.7580420792268043 2314 66-67 0.877517297444605 2314 68-69 0.9402546152384232 2314 70-71 0.9738910929332079 2314 72-73 0.9890273826580867 2314 74-75 1.0608762975144614 2314 76-77 0.7564287680902311 2314 78-79 0.80081802044959 2314 80-81 0.8705405527489276 2314 82-83 0.824057541630733 2314 84-85 0.6877263167147767 2314 86-87 0.8247243355161906 2314 88-89 0.8660774377794773 2314 90-91 1.0237436401658648 2314 92-93 1.003410261870954 2314 94-95 0.9983396332608088 2314 96-97 1.0557771163548857 2314 98-99 1.0894549016324824 2314 100 1.1894651408183385 2315 1 -0.07683617899641249 2315 2 0.09551128147457177 2315 3 0.06989812888085822 2315 4 -0.04267132405755092 2315 5 0.2185163317146106 2315 6 0.3661913928411664 2315 7 0.4299556358223242 2315 8 0.4068245023385515 2315 9 0.5777372185713503 2315 10-11 0.5496134605714147 2315 12-13 0.624672096816397 2315 14-15 0.7081954785714615 2315 16-17 0.7661902869237522 2315 18-19 0.8059380679926917 2315 20-21 0.7597754638164531 2315 22-23 0.8900323583019158 2315 24-25 0.833320680577863 2315 26-27 0.9237608802779178 2315 28-29 0.985221898240745 2315 30-31 0.9109160490282946 2315 32-33 0.948126374324751 2315 34-35 0.9754529205737299 2315 36-37 0.9908320877423762 2315 38-39 1.0198427834520771 2315 40-41 0.95477783545725 2315 42-43 1.0417715215962673 2315 44-45 0.9293797426637127 2315 46-47 0.9778737259818229 2315 48-49 1.0582134551398994 2315 50-51 0.9890692284325056 2315 52-53 1.0059638155066395 2315 54-55 0.9700397095436344 2315 56-57 1.0937552373270094 2315 58-59 1.0762804444512781 2315 60-61 1.2120359862947439 2315 62-63 1.1352082084294182 2315 64-65 1.1534321478919267 2315 66-67 1.1695596839719755 2315 68-69 1.087925859625706 2315 70-71 0.7223202934809976 2315 72-73 0.9794604357633219 2315 74-75 1.123917327952654 2315 76-77 1.056252957388466 2315 78-79 0.9232389005558801 2315 80-81 0.9440740612339553 2315 82-83 0.9229681954052076 2315 84-85 0.8630696602049461 2315 86-87 0.8354388202527296 2315 88-89 0.9307303943132261 2315 90-91 1.022554346018591 2315 92-93 1.0509544219286724 2315 94-95 1.1265473658750054 2315 96-97 1.1953540063147337 2315 98-99 1.0537913606268212 2315 100 0.906069559400084 2316 1 0.5270593117595865 2316 2 0.5119633660307485 2316 3 0.5058095044562521 2316 4 0.3406161463334456 2316 5 0.3961700887062065 2316 6 0.4101271287003385 2316 7 0.4698351531968541 2316 8 0.5216195201627158 2316 9 0.5337368047092355 2316 10-11 0.5079033244077422 2316 12-13 0.6107862149756755 2316 14-15 0.7895924780500891 2316 16-17 0.7844331899038366 2316 18-19 0.8353674267295688 2316 20-21 0.8499691886290393 2316 22-23 0.8579868304200247 2316 24-25 0.923028334690315 2316 26-27 0.9579740981749509 2316 28-29 0.9162276637038147 2316 30-31 0.8551053941535329 2316 32-33 0.7782009158545691 2316 34-35 0.7421430279785497 2316 36-37 0.7574150190877305 2316 38-39 0.6982372183541301 2316 40-41 0.7920264659802712 2316 42-43 0.9514269749003574 2316 44-45 0.8621800423378758 2316 46-47 0.8331877366785463 2316 48-49 0.5733901502833803 2316 50-51 0.6950418705134211 2316 52-53 0.8856087148711467 2316 54-55 0.9368253996818012 2316 56-57 0.9779246705290987 2316 58-59 0.905220120087499 2316 60-61 0.9010773825034093 2316 62-63 0.9969512494175632 2316 64-65 0.97153416199491 2316 66-67 0.8847175881491935 2316 68-69 0.8957339603940042 2316 70-71 0.8284253239515493 2316 72-73 0.852291285849482 2316 74-75 0.8971071847630725 2316 76-77 0.7720264985496925 2316 78-79 0.7463753507013209 2316 80-81 0.758781316913435 2316 82-83 0.6602513080128141 2316 84-85 0.6918563367045643 2316 86-87 0.7268793769755995 2316 88-89 0.6799531302241171 2316 90-91 0.78381596520234 2316 92-93 0.77400051474865 2316 94-95 0.679970248540652 2316 96-97 0.7961443133338229 2316 98-99 0.9069179107972047 2316 100 0.6146727440023092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 9.0 12 56.0 13 261.0 14 1025.0 15 2927.0 16 7178.0 17 14980.0 18 24424.0 19 33643.0 20 44740.0 21 58212.0 22 70112.0 23 74265.0 24 76342.0 25 81704.0 26 95287.0 27 116139.0 28 142146.0 29 171325.0 30 209037.0 31 256783.0 32 325103.0 33 423219.0 34 602505.0 35 801890.0 36 1198866.0 37 1890345.0 38 1765503.0 39 275674.0 40 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66394304483187 19.30220557427245 12.981595288138031 24.05225609275765 2 15.693200778024172 20.891347961337956 41.11762583500329 22.29782542563459 3 17.424026140443726 28.412830232943982 31.39024694713603 22.772896679476272 4 11.83051556571103 17.571135779297826 38.7227497203117 31.875598934679445 5 11.900962549661136 38.31605387502921 34.06821239994742 15.714771175362236 6 28.112801650711333 37.41237328322381 20.68683713351964 13.787987932545215 7 26.169243368372097 33.76191830800677 23.483318676320554 16.585519647300583 8 26.502157933359037 35.31678196982579 21.01799591930768 17.163064177507493 9 28.514736156372607 15.389131452085625 20.505818340698852 35.59031405084291 10-11 24.93822220061787 25.778680976659768 29.602834019461717 19.680262803260646 12-13 25.04191575018367 24.190284469118417 28.821941345506563 21.945858435191347 14-15 22.93550376232237 25.556355189156772 27.075393416688954 24.4327476318319 16-17 21.965577330338945 27.46256859450436 27.764021269252858 22.807832805903832 18-19 21.56256839261968 27.067068869470994 29.530304306221844 21.840058431687485 20-21 22.87682754514427 26.77498195119274 28.592522801631315 21.75566770203168 22-23 22.171429588607054 26.79451393914332 28.68159112013618 22.352465352113448 24-25 21.621725859583897 26.234327238345905 29.340509804527898 22.803437097542297 26-27 21.64756313431131 26.4013970296005 29.09776388771137 22.853275948376815 28-29 21.59474602627158 27.726590026247617 28.86415795240626 21.814505995074544 30-31 23.282804233800412 26.50130430620164 29.00366547641095 21.212225983587 32-33 22.96426105143797 26.484409566860073 28.749790155888928 21.801539225813034 34-35 22.709123149758753 27.05935968879875 28.916924033187087 21.31459312825541 36-37 22.378337725068707 27.02411669675373 28.70048482681157 21.897060751365988 38-39 22.302175269835118 27.271052662964912 28.68528135832972 21.741490708870252 40-41 22.464467019701498 26.262138888551323 29.391476192570863 21.881917899176315 42-43 22.136870795200338 26.785158311311775 28.458094967331526 22.619875926156364 44-45 21.100845558201872 27.37527415979818 29.26142281852848 22.262457463471463 46-47 21.22973708755915 26.28529829218811 28.739563907510863 23.74540071274188 48-49 21.556734088380452 26.539309046301312 28.920023963712094 22.98393290160614 50-51 21.615856942921596 27.000411054701086 28.681906654749657 22.70182534762766 52-53 21.32999138359002 27.392092304005207 28.14976005295383 23.128156259450947 54-55 21.08765126214332 26.753543621988246 29.03860557899182 23.120199536876612 56-57 21.701055096839024 26.781331028916544 28.223398266963244 23.29421560728119 58-59 21.05433191366796 27.036263517874996 28.834806833131722 23.074597735325327 60-61 21.93655851556226 27.291842524536897 28.176577229359552 22.595021730541287 62-63 21.97044748685879 27.150615485275893 27.95308897407406 22.925848053791263 64-65 21.30531407113675 26.557327936585747 27.957631004326316 24.179726987951188 66-67 21.020149991201265 26.501765654972747 27.89548208428282 24.58260226954317 68-69 20.4951204989052 26.78657921847493 28.315457403885187 24.402842878734678 70-71 20.848842701019958 26.380322783595535 28.580353584471972 24.19048093091254 72-73 20.737634349193637 26.983814513696842 27.813105508850605 24.465445628258916 74-75 20.971779622311065 26.747343929707384 27.553112132013357 24.727764315968198 76-77 20.482352844493963 26.384284328637424 28.047844129861954 25.08551869700666 78-79 20.04421029447092 26.3731981081098 28.52006781042069 25.062523786998593 80-81 20.229813168824723 26.6397871593983 27.834353952423523 25.296045719353454 82-83 20.740481605236653 26.507183559564336 27.92175840982956 24.83057642536945 84-85 20.815117751680692 26.79206418726793 27.34760030373684 25.045217757314543 86-87 19.986564258667645 26.7884468378916 27.842505804002798 25.38248309943796 88-89 19.848085064599953 26.23496321296292 28.09943917465508 25.817512547782044 90-91 20.64526796151502 25.77651463915568 28.10797335423573 25.470244045093573 92-93 20.273910531234172 26.149304837248454 27.869496301263347 25.707288330254023 94-95 19.776677758280567 25.84987556311556 27.977832444014815 26.39561423458906 96-97 19.508204437833616 25.689956435275246 28.269064595095685 26.53277453179546 98-99 19.407582842376343 25.79499708647206 28.13455631315081 26.66286375800079 100 19.212904075516033 25.85074401332937 28.309472932400077 26.626878978754526 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1305.0 1 998.5 2 2010.5 3 3166.5 4 2255.0 5 1631.5 6 2239.5 7 4045.5 8 6271.5 9 7353.0 10 6814.5 11 6200.0 12 6770.5 13 7591.5 14 8467.5 15 9575.0 16 10647.5 17 11706.5 18 12889.5 19 13899.5 20 15070.5 21 16769.0 22 19230.5 23 22991.5 24 27988.0 25 34525.0 26 43569.0 27 53901.0 28 65140.0 29 79928.0 30 98512.5 31 115992.0 32 137104.5 33 157461.5 34 175171.0 35 193817.5 36 213960.0 37 240176.5 38 252984.5 39 250704.5 40 249095.0 41 248928.5 42 252871.0 43 262804.0 44 276609.0 45 297491.5 46 316841.0 47 333227.5 48 353882.0 49 375316.0 50 395915.0 51 406418.0 52 393259.5 53 366082.0 54 338012.0 55 298584.0 56 256571.5 57 220475.5 58 186776.0 59 154906.0 60 117515.0 61 84879.5 62 64657.0 63 49029.5 64 34886.0 65 23947.5 66 17039.5 67 12755.5 68 9544.5 69 6886.0 70 5084.5 71 3769.0 72 2869.5 73 2160.5 74 1552.5 75 1067.5 76 718.5 77 434.0 78 240.5 79 152.5 80 102.0 81 68.5 82 41.0 83 26.0 84 18.5 85 12.5 86 9.0 87 4.5 88 4.0 89 4.0 90 1.5 91 1.5 92 2.0 93 2.0 94 1.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05212387682059871 2 0.0 3 0.0 4 0.014890906140735835 5 0.003948086992103141 6 1.1410656046540871E-5 7 1.7115984069811309E-4 8 3.3090902534968524E-4 9 0.0017914729993069168 10-11 8.215672353509428E-4 12-13 3.13793041279874E-4 14-15 2.2821312093081742E-5 16-17 8.557992034905654E-5 18-19 0.0 20-21 1.1410656046540871E-5 22-23 0.0 24-25 7.416926430251565E-5 26-27 1.1410656046540871E-5 28-29 9.756110919792445E-4 30-31 8.557992034905653E-4 32-33 0.014617050395618857 34-35 0.02369422728064212 36-37 0.03165886520112765 38-39 0.02121811491854275 40-41 1.5404385662830177E-4 42-43 0.001899874231749055 44-45 0.009453728534559112 46-47 0.014137802841664139 48-49 0.005568400150711945 50-51 0.010429339626538355 52-53 0.01583799059259873 54-55 0.009659120343396848 56-57 0.0031607517248918213 58-59 0.00864357195525471 60-61 0.001717303735004401 62-63 9.584951079094332E-4 64-65 0.019061500925746527 66-67 0.0451576713041855 68-69 0.05476544369537291 70-71 0.04846105622965908 72-73 0.04043936502894085 74-75 0.028908897093911298 76-77 0.028480997492166014 78-79 0.012243633937938356 80-81 0.002105266040586791 82-83 0.027533913040303126 84-85 0.04771365825861065 86-87 0.04867785869454336 88-89 0.05954080325085027 90-91 0.03447159191659997 92-93 0.026250214235067272 94-95 0.007542443646763516 96-97 0.0038853283838471663 98-99 0.023089462510175452 100 0.01975184561656225 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8763738.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.928381808505506 #Duplication Level Percentage of deduplicated Percentage of total 1 77.7484754853365 46.59340323914476 2 13.658349789095537 16.370456020700757 3 4.043274742795512 7.269207376288089 4 1.6254951214675013 3.8965316906866976 5 0.8282543459297066 2.4817971338714724 6 0.4870228479943048 1.751189471044106 7 0.320173912257408 1.343125312121942 8 0.21929799084262347 1.0513738980043905 9 0.16095187315577136 0.8681026776545891 >10 0.8284415658367106 8.776455960885547 >50 0.04800533903315311 1.9934707918021746 >100 0.02779793090508892 3.227576763900474 >500 0.0027276924676873566 1.1361868226035794 >1k 0.0015416244863842828 1.7519287186677668 >5k 9.486919804614818E-5 0.34831342860838355 >10k+ 9.486919804614818E-5 1.1408806940152505 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29258 0.3338529746096928 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22745 0.2595353717785721 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17011 0.19410667000770676 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 11254 0.12841552314777097 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1410656046540871E-5 2 0.0 0.0 0.0 0.0 1.1410656046540871E-5 3 0.0 0.0 0.0 0.0 1.1410656046540871E-5 4 0.0 0.0 0.0 0.0 1.1410656046540871E-5 5 0.0 1.1410656046540871E-5 0.0 0.0 1.1410656046540871E-5 6 0.0 1.1410656046540871E-5 0.0 1.1410656046540871E-5 1.1410656046540871E-5 7 0.0 2.2821312093081742E-5 0.0 1.1410656046540871E-5 1.1410656046540871E-5 8 0.0 2.2821312093081742E-5 0.0 1.1410656046540871E-5 1.1410656046540871E-5 9 0.0 2.2821312093081742E-5 0.0 3.423196813962261E-5 2.2821312093081742E-5 10-11 0.0 2.2821312093081742E-5 0.0 6.846393627924523E-5 2.2821312093081742E-5 12-13 0.0 2.8526640116352178E-5 0.0 6.846393627924523E-5 2.8526640116352178E-5 14-15 0.0 6.275860825597479E-5 0.0 7.416926430251565E-5 3.9937296162893045E-5 16-17 0.0 9.128524837232697E-5 0.0 1.2551721651194957E-4 4.5642624186163484E-5 18-19 0.0 9.128524837232697E-5 0.0 2.3391844895408786E-4 4.5642624186163484E-5 20-21 0.0 9.128524837232697E-5 0.0 6.561127226761001E-4 4.5642624186163484E-5 22-23 0.0 9.128524837232697E-5 0.0 0.0019854541520981117 4.5642624186163484E-5 24-25 0.0 9.699057639559741E-5 0.0 0.004917992756059116 5.134795220943392E-5 26-27 5.7053280232704355E-6 1.0269590441886783E-4 0.0 0.008563697362928923 6.846393627924523E-5 28-29 1.1410656046540871E-5 1.0269590441886783E-4 0.0 0.019962942753423252 6.846393627924523E-5 30-31 1.1410656046540871E-5 1.0269590441886783E-4 0.0 0.0633177304022553 6.846393627924523E-5 32-33 1.1410656046540871E-5 1.0269590441886783E-4 0.0 0.16156347896297218 6.846393627924523E-5 34-35 2.2821312093081742E-5 1.0269590441886783E-4 0.0 0.2896024504612073 6.846393627924523E-5 36-37 3.423196813962261E-5 1.1981188848867914E-4 0.0 0.4738674296287726 6.846393627924523E-5 38-39 4.5642624186163484E-5 1.2551721651194957E-4 0.0 0.8259489272728144 7.987459232578609E-5 40-41 4.5642624186163484E-5 1.2551721651194957E-4 0.0 1.304180932839389 9.128524837232697E-5 42-43 4.5642624186163484E-5 1.2551721651194957E-4 0.0 1.7349560199083998 9.128524837232697E-5 44-45 5.134795220943392E-5 1.2551721651194957E-4 0.0 2.170774616949982 9.128524837232697E-5 46-47 5.7053280232704355E-5 1.2551721651194957E-4 0.0 2.650695399611444 9.128524837232697E-5 48-49 5.7053280232704355E-5 1.2551721651194957E-4 0.0 3.1793625049037297 1.1410656046540871E-4 50-51 5.7053280232704355E-5 1.3122254453522001E-4 0.0 3.7683292220739597 1.1410656046540871E-4 52-53 5.7053280232704355E-5 1.3692787255849045E-4 0.0 4.3790560603249435 1.426332005817609E-4 54-55 5.7053280232704355E-5 1.483385286050313E-4 0.0 5.054338685159232 1.4833852860503133E-4 56-57 5.7053280232704355E-5 1.5974918465157218E-4 0.0 5.70123730307775 1.4833852860503133E-4 58-59 5.7053280232704355E-5 1.5974918465157218E-4 0.0 6.430520857652294 1.4833852860503133E-4 60-61 5.7053280232704355E-5 1.5974918465157218E-4 0.0 7.156483911317294 1.4833852860503133E-4 62-63 5.7053280232704355E-5 1.5974918465157218E-4 0.0 7.991892272452691 1.5974918465157218E-4 64-65 5.7053280232704355E-5 1.6545451267484262E-4 0.0 8.730184539975978 1.5974918465157218E-4 66-67 5.7053280232704355E-5 1.7115984069811306E-4 0.0 9.483156616503141 1.5974918465157218E-4 68-69 5.7053280232704355E-5 1.7115984069811306E-4 0.0 10.326033252020999 1.5974918465157218E-4 70-71 5.7053280232704355E-5 1.7115984069811306E-4 0.0 11.195325556286598 1.5974918465157218E-4 72-73 5.7053280232704355E-5 1.7115984069811306E-4 0.0 11.98344815876513 1.7115984069811306E-4 74-75 5.7053280232704355E-5 1.768651687213835E-4 0.0 12.764718662287713 1.7115984069811306E-4 76-77 5.7053280232704355E-5 2.0539180883773567E-4 0.0 13.58112828110562 1.7115984069811306E-4 78-79 6.846393627924523E-5 2.0539180883773567E-4 0.0 14.43147889633396 1.7115984069811306E-4 80-81 6.846393627924523E-5 2.0539180883773567E-4 0.0 15.244841869987441 1.768651687213835E-4 82-83 6.846393627924523E-5 2.0539180883773567E-4 0.0 16.10082364397475 1.8257049674465394E-4 84-85 6.846393627924523E-5 2.0539180883773567E-4 0.0 16.937703979740153 1.8257049674465394E-4 86-87 6.846393627924523E-5 2.0539180883773567E-4 0.0 17.773391901948692 1.8827582476792438E-4 88 6.846393627924523E-5 2.0539180883773567E-4 0.0 18.383217298371996 1.9398115279119481E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 10960 0.0 60.75918 2 ACCTGGG 12215 0.0 53.517235 3 GGTATCA 13950 0.0 52.143684 1 CCTGGGG 10695 0.0 45.129147 4 GTATCAA 25310 0.0 41.37268 1 CTGGGGG 7670 0.0 39.086193 5 TAACGCA 1250 0.0 38.72529 4 GTACCTG 18500 0.0 36.523354 1 TGGGGGG 22430 0.0 34.041393 6 ATAACGC 1310 0.0 33.72225 3 GTACATG 32800 0.0 32.641853 1 ATCAACG 31870 0.0 32.220284 3 CAACGCA 32535 0.0 31.716087 5 TACATGG 33375 0.0 31.484957 2 TATCAAC 33165 0.0 31.259218 2 TCAACGC 33325 0.0 30.983278 4 ATGGGGG 20480 0.0 30.676054 5 ACATGGG 33190 0.0 30.61318 3 CATGGGG 26340 0.0 30.27844 4 AACGCAG 34930 0.0 30.159227 6 >>END_MODULE