FastQCFastQC Report
Mon 6 Jun 2016
ERR1211433_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211433_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8589519
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299760.3489834529733271No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224260.261085632385236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158140.184108097321864No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG115500.059.2078132
ACCTGGG133450.050.2662853
GGTATCA162650.043.8203161
GTACCTG169500.041.0766831
CCTGGGG117950.038.93754
GTATCAA275800.037.4742551
TAACGCA11200.036.5151144
ATAACGC11450.034.486193
TATCACG8100.031.9575162
CTGGGGG83700.031.5071285
GTACATG291450.031.1625961
ATCAACG331550.030.5966473
TACATGG293100.030.5093632
CAACGCA332900.030.4019645
TATCAAC340400.029.961582
TCAACGC341150.029.7494324
AACGCAG353050.029.0532686
ACATGGG298300.028.8225153
TGGGGGG196600.028.8124376
CATGGGG223150.028.1226224
Produced by FastQC (version 0.11.5)