##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211433_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8589519 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.173965620193634 31.0 31.0 34.0 28.0 34.0 2 31.212778852925293 31.0 31.0 34.0 28.0 34.0 3 31.27362440201832 31.0 31.0 34.0 28.0 34.0 4 34.89878781338047 37.0 35.0 37.0 32.0 37.0 5 34.9617294053369 37.0 35.0 37.0 32.0 37.0 6 35.02164114195451 37.0 35.0 37.0 32.0 37.0 7 34.95175154743822 37.0 35.0 37.0 32.0 37.0 8 34.9477472487109 37.0 35.0 37.0 32.0 37.0 9 36.49198366055189 39.0 37.0 39.0 32.0 39.0 10-11 36.406157259795336 39.0 37.0 39.0 32.0 39.0 12-13 36.30710503114319 39.0 35.0 39.0 32.0 39.0 14-15 37.45499497701792 40.0 37.0 41.0 31.5 41.0 16-17 37.445032079211884 40.0 37.0 41.0 31.0 41.0 18-19 37.450948010010805 40.0 37.0 41.0 31.0 41.0 20-21 37.43586765452174 40.0 37.0 41.0 31.0 41.0 22-23 37.30945056411191 40.0 37.0 41.0 31.0 41.0 24-25 37.22029417479605 40.0 36.0 41.0 31.0 41.0 26-27 37.08357708970665 40.0 36.0 41.0 30.5 41.0 28-29 36.94441854078209 39.5 36.0 41.0 30.0 41.0 30-31 36.78214734724959 39.0 36.0 41.0 30.0 41.0 32-33 36.57019682941501 39.0 35.0 41.0 30.0 41.0 34-35 36.441694174027674 39.0 35.0 41.0 29.5 41.0 36-37 36.31472472439958 39.0 35.0 41.0 29.0 41.0 38-39 36.164375851546524 39.0 35.0 40.0 28.5 41.0 40-41 36.01046548706627 39.0 35.0 40.0 28.0 41.0 42-43 35.82076801972264 38.0 35.0 40.0 27.0 41.0 44-45 35.69416750809911 38.0 35.0 40.0 27.0 41.0 46-47 35.47754769504556 38.0 34.0 40.0 26.0 41.0 48-49 35.2357260051465 38.0 34.0 40.0 25.0 41.0 50-51 34.58645821727619 37.5 33.5 39.5 24.5 40.5 52-53 34.90293449493505 38.0 33.5 39.5 25.5 40.5 54-55 35.318386687310436 38.0 34.0 40.0 26.0 41.0 56-57 35.254235015953746 38.0 34.0 40.0 26.0 41.0 58-59 35.06554674365351 38.0 34.0 40.0 25.0 41.0 60-61 34.84980381322866 38.0 34.0 40.0 25.0 41.0 62-63 34.57354439753844 37.0 33.0 40.0 25.0 41.0 64-65 34.382347137249475 37.0 33.0 40.0 24.5 41.0 66-67 34.19688617022676 36.5 33.0 39.5 24.5 41.0 68-69 33.88053475404152 36.0 33.0 39.0 23.5 41.0 70-71 33.52806169938037 35.5 32.0 39.0 23.0 41.0 72-73 33.16542166098009 35.0 32.0 38.5 23.0 40.0 74-75 32.815525060250756 35.0 32.0 37.5 22.0 40.0 76-77 32.46104182318009 35.0 31.5 37.0 22.0 39.0 78-79 32.1002002556837 35.0 31.0 36.5 20.5 39.0 80-81 31.746381258368483 35.0 31.0 36.0 20.0 38.5 82-83 31.41646790699223 35.0 31.0 35.5 20.0 37.0 84-85 31.114443369879034 34.0 30.0 35.0 19.0 37.0 86-87 30.821070947046046 34.0 30.0 35.0 18.5 36.5 88-89 30.558936361861473 34.0 30.0 35.0 18.0 36.0 90-91 30.3051739567722 34.0 29.0 35.0 17.5 36.0 92-93 30.065503726110855 34.0 29.0 35.0 16.5 35.5 94-95 29.82706167830818 34.0 29.0 35.0 15.0 35.0 96-97 29.577793296691002 34.0 29.0 35.0 12.0 35.0 98-99 29.3159014491964 34.0 27.5 35.0 10.0 35.0 100 28.107966231869327 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3394081161411435 1101 2 -0.3557219773298108 1101 3 -0.44314631326513165 1101 4 -0.24703541256886297 1101 5 -0.20170089788528145 1101 6 -0.2808057661258445 1101 7 -0.2466096510697895 1101 8 -0.3436499539082831 1101 9 -0.4734538364770273 1101 10-11 -0.4922708017849047 1101 12-13 -0.4924742541940006 1101 14-15 -0.7066627885034364 1101 16-17 -0.6849099448665399 1101 18-19 -0.6703153613000694 1101 20-21 -0.7107150651328524 1101 22-23 -0.6859292394421885 1101 24-25 -0.7730207857984865 1101 26-27 -0.8224045340492765 1101 28-29 -0.8405649672583664 1101 30-31 -0.8329130244390868 1101 32-33 -0.8320249294179902 1101 34-35 -0.8533425876957921 1101 36-37 -0.8671895068291775 1101 38-39 -0.9170865070370269 1101 40-41 -1.0928413870673168 1101 42-43 -1.0821666035965976 1101 44-45 -1.0874579902816635 1101 46-47 -1.1800204068702698 1101 48-49 -1.2307157289458601 1101 50-51 -1.0211278614537775 1101 52-53 -0.7826989820696753 1101 54-55 -0.8146245771464962 1101 56-57 -0.8758250218650332 1101 58-59 -0.9218812453584562 1101 60-61 -0.7952888123057775 1101 62-63 -0.9220114012337817 1101 64-65 -0.8635601586556874 1101 66-67 -0.9130133919668424 1101 68-69 -0.9944053131289721 1101 70-71 -0.9096952190491479 1101 72-73 -1.0077773488219037 1101 74-75 -1.111673534591688 1101 76-77 -0.9696829311749013 1101 78-79 -0.9863567030290028 1101 80-81 -1.172781969861603 1101 82-83 -1.1377500070013973 1101 84-85 -1.1697076889580096 1101 86-87 -1.2732836991277239 1101 88-89 -1.419195906035803 1101 90-91 -1.3746772044471918 1101 92-93 -1.524638691799737 1101 94-95 -1.6197538096724458 1101 96-97 -1.8311604459813289 1101 98-99 -1.7928385452924296 1101 100 -1.7062569519381512 1102 1 -0.405959167193231 1102 2 -0.47722232043979673 1102 3 -0.470557135014289 1102 4 -0.4719578818173389 1102 5 -0.397877029936879 1102 6 -0.383860769020103 1102 7 -0.5109066927054542 1102 8 -0.42623095679602585 1102 9 -0.5620898975553033 1102 10-11 -0.6758607463876345 1102 12-13 -0.7078262393815749 1102 14-15 -0.780345859482054 1102 16-17 -0.8162913818876731 1102 18-19 -0.8449933525535229 1102 20-21 -0.9318255118771575 1102 22-23 -0.8532632526467836 1102 24-25 -0.9396466710447839 1102 26-27 -0.9636971308966409 1102 28-29 -0.9895227934966542 1102 30-31 -0.8936464196082099 1102 32-33 -0.9072409426559389 1102 34-35 -0.9379150051529308 1102 36-37 -0.9603350275357982 1102 38-39 -1.0719631491203643 1102 40-41 -1.2178192529287912 1102 42-43 -1.2091163280247272 1102 44-45 -1.213502661586702 1102 46-47 -1.243544011064273 1102 48-49 -1.288808669005185 1102 50-51 -1.083739734865219 1102 52-53 -0.9056279495689807 1102 54-55 -0.9263526014708034 1102 56-57 -0.8707478296017683 1102 58-59 -0.8751951950581756 1102 60-61 -0.9501701830272964 1102 62-63 -0.9084731209916228 1102 64-65 -1.087101331015191 1102 66-67 -1.0271524789952693 1102 68-69 -0.9812609078875454 1102 70-71 -1.0229329289566849 1102 72-73 -0.999347420512656 1102 74-75 -1.1067589087970333 1102 76-77 -1.0211170605214264 1102 78-79 -1.1074493141724773 1102 80-81 -1.069879097537104 1102 82-83 -1.1938918122648552 1102 84-85 -1.1901328071813637 1102 86-87 -1.327621853764093 1102 88-89 -1.4100540668577253 1102 90-91 -1.445740938825292 1102 92-93 -1.414630904925101 1102 94-95 -1.4543500512422725 1102 96-97 -1.6662798311840241 1102 98-99 -1.7490086993585336 1102 100 -1.6919298855059992 1103 1 -0.37400439735899127 1103 2 -0.46050927907479533 1103 3 -0.5614440311849158 1103 4 -0.35092598264694885 1103 5 -0.3172039657719026 1103 6 -0.3299225059393578 1103 7 -0.3225898299390195 1103 8 -0.35687958489102556 1103 9 -0.4324349840837627 1103 10-11 -0.4757264350286263 1103 12-13 -0.5995206308886196 1103 14-15 -0.6919300866930627 1103 16-17 -0.7161693836850205 1103 18-19 -0.7820877277563625 1103 20-21 -0.7330213695079308 1103 22-23 -0.7055688851022239 1103 24-25 -0.6972425811015839 1103 26-27 -0.7680414164627791 1103 28-29 -0.8025640532564111 1103 30-31 -0.8087320357124881 1103 32-33 -0.7895535671384266 1103 34-35 -0.7708514193173244 1103 36-37 -0.8264791015034589 1103 38-39 -0.8640213980405775 1103 40-41 -0.8880088018681676 1103 42-43 -0.9169980352644913 1103 44-45 -0.8555831931912365 1103 46-47 -1.0190388465411573 1103 48-49 -0.991174017435533 1103 50-51 -0.8911421923639864 1103 52-53 -0.6966870680198554 1103 54-55 -0.7066380643123154 1103 56-57 -0.732347628381639 1103 58-59 -0.7398729833950952 1103 60-61 -0.8533092121628414 1103 62-63 -0.7568518846908248 1103 64-65 -0.6766823364050509 1103 66-67 -0.7407212534783909 1103 68-69 -0.7021520679236843 1103 70-71 -0.6791413786315346 1103 72-73 -0.7177001112449517 1103 74-75 -0.8696780529703325 1103 76-77 -0.9157414570371216 1103 78-79 -0.8673497921350446 1103 80-81 -0.9488379533801563 1103 82-83 -0.9752857587091874 1103 84-85 -1.0421607581272383 1103 86-87 -1.0716092488482687 1103 88-89 -1.14242277246489 1103 90-91 -1.1184427683368945 1103 92-93 -1.1759395352010067 1103 94-95 -1.1350284179170558 1103 96-97 -1.2880330740735424 1103 98-99 -1.3874088034334413 1103 100 -1.5726183957606068 1104 1 -0.525829141650032 1104 2 -0.44603375264545164 1104 3 -0.4718760221030607 1104 4 -0.27022531207929745 1104 5 -0.3586192179058898 1104 6 -0.3501177991070321 1104 7 -0.3434918979133812 1104 8 -0.4778937055186958 1104 9 -0.47203667595138654 1104 10-11 -0.5680244735189888 1104 12-13 -0.5961846381156732 1104 14-15 -0.8450291889157029 1104 16-17 -0.8254665024547876 1104 18-19 -0.8621473633613945 1104 20-21 -0.8477931377945467 1104 22-23 -0.8506419423538105 1104 24-25 -0.8481947100448153 1104 26-27 -0.852164905087669 1104 28-29 -0.972360896142682 1104 30-31 -0.941003722712729 1104 32-33 -0.9408886243419801 1104 34-35 -0.9098357595587174 1104 36-37 -1.0547700273135874 1104 38-39 -0.9695214464856221 1104 40-41 -1.0349310546787578 1104 42-43 -0.9910818316306091 1104 44-45 -1.0000449524048207 1104 46-47 -0.9493453231289664 1104 48-49 -0.9586209080234198 1104 50-51 -0.8834056249168043 1104 52-53 -0.7770303399671263 1104 54-55 -0.7152945075768002 1104 56-57 -0.7269587502855543 1104 58-59 -0.8376246104705345 1104 60-61 -0.8335521464979934 1104 62-63 -0.945716631844455 1104 64-65 -0.8314163812787214 1104 66-67 -0.7503620134561473 1104 68-69 -0.7733282711294933 1104 70-71 -0.7643718487837532 1104 72-73 -0.803384151192418 1104 74-75 -0.7974503962252868 1104 76-77 -0.7717313722983228 1104 78-79 -0.7764236960720332 1104 80-81 -0.8110195374988081 1104 82-83 -0.8184379740202026 1104 84-85 -0.8287517497670756 1104 86-87 -0.9443736244047152 1104 88-89 -0.8963925048505423 1104 90-91 -0.9457928962791939 1104 92-93 -1.0274085964634807 1104 94-95 -1.0262212149803567 1104 96-97 -1.08058069849357 1104 98-99 -1.121941869178027 1104 100 -1.1624538084184373 1105 1 -0.48594989781453535 1105 2 -0.5052579214782078 1105 3 -0.5135431266988881 1105 4 -0.4038133123720229 1105 5 -0.4174548646911376 1105 6 -0.4197120107911303 1105 7 -0.483620666712703 1105 8 -0.3920716887644957 1105 9 -0.5896301214933786 1105 10-11 -0.6337277199678297 1105 12-13 -0.624822904080709 1105 14-15 -0.859215009189441 1105 16-17 -0.7430573791750419 1105 18-19 -0.7339166293775463 1105 20-21 -0.8427247088878786 1105 22-23 -0.7668946490008324 1105 24-25 -0.775458604155304 1105 26-27 -0.8175699266739329 1105 28-29 -0.8050269015961575 1105 30-31 -0.8519739591755453 1105 32-33 -0.9258812870428841 1105 34-35 -0.8884310279522794 1105 36-37 -0.7816411495596682 1105 38-39 -0.8885671347591355 1105 40-41 -0.933003668055683 1105 42-43 -0.8037146669693982 1105 44-45 -0.8059331455321725 1105 46-47 -0.9837090912660074 1105 48-49 -0.9314004064366586 1105 50-51 -0.8700306155244419 1105 52-53 -0.7792200401735556 1105 54-55 -0.7139434770745368 1105 56-57 -0.7158531170063682 1105 58-59 -0.6983216835870323 1105 60-61 -0.6572976165424365 1105 62-63 -0.7693361904549434 1105 64-65 -0.7447067174758004 1105 66-67 -0.7160529511034994 1105 68-69 -0.6656349568487414 1105 70-71 -0.6793648372386798 1105 72-73 -0.6986574343300447 1105 74-75 -0.6669535175558607 1105 76-77 -0.7392888837126037 1105 78-79 -0.7902993156166715 1105 80-81 -0.8097936024134249 1105 82-83 -0.7766086452308016 1105 84-85 -0.6977619315058305 1105 86-87 -0.8175333182272766 1105 88-89 -0.8469824590798183 1105 90-91 -0.8764538694533179 1105 92-93 -0.8659547719644678 1105 94-95 -1.04600336192388 1105 96-97 -1.1446445666481893 1105 98-99 -1.1119824624367958 1105 100 -1.1058980480462246 1106 1 -0.48902825191141375 1106 2 -0.5549371033520707 1106 3 -0.5727210007914749 1106 4 -0.40947898636869695 1106 5 -0.43500780265569716 1106 6 -0.5359462520728542 1106 7 -0.5158608353900576 1106 8 -0.45457707570128747 1106 9 -0.6686998262132278 1106 10-11 -0.7736625748466963 1106 12-13 -0.6916151954385583 1106 14-15 -0.8699271496704526 1106 16-17 -0.8106690733139459 1106 18-19 -0.9122830760589409 1106 20-21 -0.8672146220296213 1106 22-23 -0.8285227166287612 1106 24-25 -0.9084446365716374 1106 26-27 -0.8435250389159066 1106 28-29 -0.9409616835784291 1106 30-31 -0.9174020952601083 1106 32-33 -0.9835077710465754 1106 34-35 -0.9322951646197666 1106 36-37 -0.965939667182802 1106 38-39 -1.0179099758596521 1106 40-41 -1.0058477754004187 1106 42-43 -0.8981265585015663 1106 44-45 -0.9684456980888001 1106 46-47 -1.0193479360116129 1106 48-49 -0.9677399266715341 1106 50-51 -0.9487127311360126 1106 52-53 -0.8873897468589433 1106 54-55 -0.8020460928215627 1106 56-57 -0.8754095094803063 1106 58-59 -0.8554198518154337 1106 60-61 -0.7772560082402151 1106 62-63 -0.9135126915346277 1106 64-65 -0.8888791952491815 1106 66-67 -0.7724706621494519 1106 68-69 -0.8302722444952977 1106 70-71 -0.8302681474242704 1106 72-73 -0.806589638724688 1106 74-75 -0.7703474801538093 1106 76-77 -0.8764809091569497 1106 78-79 -0.8340045555389892 1106 80-81 -0.8919028593828564 1106 82-83 -0.8460221126904592 1106 84-85 -0.8163687626282972 1106 86-87 -0.8801607719590976 1106 88-89 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36-37 1.264152635030264 2310 38-39 1.3361784438201383 2310 40-41 1.331277448835884 2310 42-43 1.3632064487332016 2310 44-45 1.4182201495997901 2310 46-47 1.397292521863811 2310 48-49 1.471043775445736 2310 50-51 1.3512017563076029 2310 52-53 1.2990055843092279 2310 54-55 1.2706781283009292 2310 56-57 1.2598280648160838 2310 58-59 1.2768251188580848 2310 60-61 1.2303696927050112 2310 62-63 1.2305165577251884 2310 64-65 1.2330072700085921 2310 66-67 1.1815944942738597 2310 68-69 1.1940553476325206 2310 70-71 1.111615779797532 2310 72-73 1.0917432507881983 2310 74-75 1.0499735303954907 2310 76-77 1.073226336032647 2310 78-79 1.0559708890501724 2310 80-81 1.127760090337361 2310 82-83 1.1576709759206842 2310 84-85 1.1472173249541946 2310 86-87 1.1970369801003145 2310 88-89 1.2468269669646972 2310 90-91 1.2971959301456302 2310 92-93 1.284948343241858 2310 94-95 1.4068825452924365 2310 96-97 1.3864438812475868 2310 98-99 1.4379169504237161 2310 100 1.5052977497024038 2311 1 0.6357888344886291 2311 2 0.6774434241525924 2311 3 0.752888894809697 2311 4 0.6379499291100927 2311 5 0.5790521753544624 2311 6 0.6126283356861535 2311 7 0.6391758718054064 2311 8 0.6605805643980389 2311 9 0.7885696722788111 2311 10-11 0.7341979269811603 2311 12-13 0.8662038436082753 2311 14-15 1.0503902789749162 2311 16-17 1.0509606766235393 2311 18-19 1.055022736908441 2311 20-21 1.0651447259728215 2311 22-23 1.1165621513730883 2311 24-25 1.1435657792872291 2311 26-27 1.2082078962120804 2311 28-29 1.2468184780430178 2311 30-31 1.2337751377770232 2311 32-33 1.2343956860232694 2311 34-35 1.2602228136844076 2311 36-37 1.2586885712489746 2311 38-39 1.2552933108903943 2311 40-41 1.3063079269891773 2311 42-43 1.3663887383519082 2311 44-45 1.3742955892191446 2311 46-47 1.4631656806718922 2311 48-49 1.4773529202083182 2311 50-51 1.4012120483339032 2311 52-53 1.2395865969502893 2311 54-55 1.14315929855983 2311 56-57 1.1745920526869185 2311 58-59 1.1780812225946349 2311 60-61 1.149155160894452 2311 62-63 1.2079589857512545 2311 64-65 1.1676759384866529 2311 66-67 1.20157449581054 2311 68-69 1.1959238932547436 2311 70-71 1.1598355294443579 2311 72-73 1.163199590686233 2311 74-75 1.1668223782092824 2311 76-77 1.1666917541365152 2311 78-79 1.1772347459494483 2311 80-81 1.1961786760370785 2311 82-83 1.2161613228351662 2311 84-85 1.1994416455379913 2311 86-87 1.2695120193573928 2311 88-89 1.3384380714273405 2311 90-91 1.4222811795644112 2311 92-93 1.4109429214318823 2311 94-95 1.4260561677968298 2311 96-97 1.5037923074567559 2311 98-99 1.514906833877692 2311 100 1.6339472924023752 2312 1 0.5857613842141056 2312 2 0.6046331330169821 2312 3 0.6815307165807489 2312 4 0.5052408602185992 2312 5 0.4628308444500888 2312 6 0.40972451171565183 2312 7 0.49346220901748694 2312 8 0.5700746447878728 2312 9 0.7478747076779797 2312 10-11 0.8029123372457221 2312 12-13 0.8490744214567698 2312 14-15 1.0727036792222577 2312 16-17 1.0596582898585538 2312 18-19 1.0219685487482906 2312 20-21 1.0591063418494073 2312 22-23 1.0935628838541191 2312 24-25 1.101418393046849 2312 26-27 1.12347199833426 2312 28-29 1.1551261959389691 2312 30-31 1.06838247780842 2312 32-33 1.1345431819373317 2312 34-35 1.169791974973279 2312 36-37 1.1935166062930023 2312 38-39 1.2204420473716269 2312 40-41 1.2433058691129304 2312 42-43 1.2828842926717599 2312 44-45 1.316305123772949 2312 46-47 1.261181165243599 2312 48-49 1.3537420185264608 2312 50-51 1.2376892119744412 2312 52-53 1.1244763222167293 2312 54-55 1.0857515541961575 2312 56-57 1.0456006619731468 2312 58-59 1.0688626348555132 2312 60-61 0.9964241954901283 2312 62-63 1.0512755042498227 2312 64-65 1.1205256190387658 2312 66-67 1.0683062822483365 2312 68-69 1.1228935539754232 2312 70-71 1.0796341875735962 2312 72-73 1.100336570422911 2312 74-75 1.0824081064971836 2312 76-77 1.0998086297753247 2312 78-79 1.0908040564102208 2312 80-81 0.9275873129818422 2312 82-83 1.1185730118741013 2312 84-85 1.21529853441508 2312 86-87 1.2777036193822688 2312 88-89 1.2856731846090632 2312 90-91 1.2842033926950762 2312 92-93 1.306702631975682 2312 94-95 1.4102340530245456 2312 96-97 1.4375675633387424 2312 98-99 1.3313272729948586 2312 100 1.5239542408526532 2313 1 0.5812539173968787 2313 2 0.6128080145247523 2313 3 0.7392673626910238 2313 4 0.5823952665355989 2313 5 0.6009333261130365 2313 6 0.6298616540259232 2313 7 0.6253731869934498 2313 8 0.6867804680223131 2313 9 0.8498187810256326 2313 10-11 0.7287915389457211 2313 12-13 0.883442918758881 2313 14-15 1.0794395390638911 2313 16-17 1.0715739477423156 2313 18-19 1.056745939131396 2313 20-21 1.0643821211942495 2313 22-23 1.0958380391766696 2313 24-25 1.1599095733812916 2313 26-27 1.2286611592098282 2313 28-29 1.194200276218119 2313 30-31 1.143153100884902 2313 32-33 1.228145440341791 2313 34-35 1.2296497442157843 2313 36-37 1.2532605088443844 2313 38-39 1.279301837403679 2313 40-41 1.3037522293713195 2313 42-43 1.3250444818681473 2313 44-45 1.2834375561951106 2313 46-47 1.3296233550802015 2313 48-49 1.3417733269059582 2313 50-51 1.3160616212584841 2313 52-53 1.22804792818701 2313 54-55 1.2347104289027797 2313 56-57 1.188664134486423 2313 58-59 1.2183209329811646 2313 60-61 1.129348623796865 2313 62-63 1.126925312286744 2313 64-65 1.1478608158412769 2313 66-67 1.1316899768682518 2313 68-69 1.151946144156426 2313 70-71 1.126646895309463 2313 72-73 1.1189643981426656 2313 74-75 1.1187425253300916 2313 76-77 1.1114191137986467 2313 78-79 1.1493031397003648 2313 80-81 1.1880384319889785 2313 82-83 1.2175830233251794 2313 84-85 1.2447556701083577 2313 86-87 1.2711467313089564 2313 88-89 1.3224697283051405 2313 90-91 1.3505637384095905 2313 92-93 1.4230564860439472 2313 94-95 1.5012796533901636 2313 96-97 1.5787305541895016 2313 98-99 1.6310853076908494 2313 100 1.7086518703252551 2314 1 0.548915093254358 2314 2 0.5398653725980473 2314 3 0.6033980508079537 2314 4 0.557044673517062 2314 5 0.5145029681928364 2314 6 0.5330108304966856 2314 7 0.6421696425234984 2314 8 0.5903817928265127 2314 9 0.73713329385658 2314 10-11 0.6744158046204589 2314 12-13 0.4797885905456454 2314 14-15 0.883722790387445 2314 16-17 0.911005079498473 2314 18-19 0.9687223692225757 2314 20-21 0.9767381902181782 2314 22-23 0.8757951029601116 2314 24-25 0.8931421349717823 2314 26-27 0.9801606627376529 2314 28-29 1.0611028790794066 2314 30-31 0.9703729889249288 2314 32-33 1.003145379299184 2314 34-35 1.074409876954192 2314 36-37 1.1122886202769493 2314 38-39 1.1404563875586007 2314 40-41 1.205327750521981 2314 42-43 1.3002615390055396 2314 44-45 1.2422392893996772 2314 46-47 1.22802298843758 2314 48-49 1.272677331950419 2314 50-51 1.1856358032495677 2314 52-53 1.0327056355806405 2314 54-55 1.0226365425652162 2314 56-57 0.9867945123687107 2314 58-59 1.0254816267402802 2314 60-61 1.012293808320834 2314 62-63 0.995884410562617 2314 64-65 0.9545411572799765 2314 66-67 0.9667630536196228 2314 68-69 0.9902746161181355 2314 70-71 1.0380640922136948 2314 72-73 1.0155746585246135 2314 74-75 1.0061627317038528 2314 76-77 0.9985837336338221 2314 78-79 1.0931744875040366 2314 80-81 1.1069781647863763 2314 82-83 0.9535368227804675 2314 84-85 0.9638354139100151 2314 86-87 1.1385761571892061 2314 88-89 1.2527637679992338 2314 90-91 1.3249190904938892 2314 92-93 1.413949523690281 2314 94-95 1.3820118338674803 2314 96-97 1.5534758878969868 2314 98-99 1.5927765444283075 2314 100 1.460112430974629 2315 1 0.4195399096020047 2315 2 0.4413732007442803 2315 3 0.48150478276995656 2315 4 0.41690857828274375 2315 5 0.3660857225820706 2315 6 -0.3484561157201398 2315 7 0.2604573042786811 2315 8 0.3614779987123313 2315 9 0.5176823112309634 2315 10-11 0.6160072996751325 2315 12-13 0.6834945101220313 2315 14-15 0.7675191799290815 2315 16-17 0.6273382493907107 2315 18-19 0.7574179042764939 2315 20-21 0.7820380636077857 2315 22-23 0.7262060395697176 2315 24-25 0.8184732967089232 2315 26-27 0.8492769597588179 2315 28-29 0.9698078427535037 2315 30-31 0.9469176396989667 2315 32-33 1.0180019854318232 2315 34-35 1.0146149750167766 2315 36-37 1.0481787445506967 2315 38-39 1.0955704371380648 2315 40-41 1.1077817767828648 2315 42-43 1.1454083731074007 2315 44-45 1.2494874063827623 2315 46-47 1.24878431979635 2315 48-49 1.2301616090973013 2315 50-51 1.1126168491057342 2315 52-53 1.0062782066730307 2315 54-55 0.8440643974702624 2315 56-57 0.7961824819341103 2315 58-59 0.7550476729067839 2315 60-61 0.6846768121247635 2315 62-63 0.854067535452117 2315 64-65 0.8966314863366094 2315 66-67 0.9657362731035946 2315 68-69 1.000297255036024 2315 70-71 1.0010733371181644 2315 72-73 0.9695588587892132 2315 74-75 1.0250967852285697 2315 76-77 0.9429356459383413 2315 78-79 0.8654319775824888 2315 80-81 0.9490549789651936 2315 82-83 1.066622450014325 2315 84-85 1.0037529855965808 2315 86-87 1.083770060666069 2315 88-89 1.251666284351522 2315 90-91 1.3203702443478953 2315 92-93 1.3714531999628292 2315 94-95 1.4023137423221357 2315 96-97 1.489465081339862 2315 98-99 1.562486754206585 2315 100 1.602190079177614 2316 1 -0.06108895570607942 2316 2 -0.08354050824438986 2316 3 0.05314716246276063 2316 4 0.020523132157059365 2316 5 0.03517784521111622 2316 6 0.15086328405222105 2316 7 0.11305523631136083 2316 8 0.1309916393240229 2316 9 0.24840439665492653 2316 10-11 0.15819069059805457 2316 12-13 0.19975962902948652 2316 14-15 0.4026742104948937 2316 16-17 0.3937627323153805 2316 18-19 0.3777986374258546 2316 20-21 0.354509933526451 2316 22-23 0.2514146956761323 2316 24-25 0.33743023849722675 2316 26-27 0.39004396072133574 2316 28-29 0.5219440478841619 2316 30-31 0.5147966590240642 2316 32-33 0.629359676709143 2316 34-35 0.5706289466128567 2316 36-37 0.6383572241688569 2316 38-39 0.6586882448810911 2316 40-41 0.658072552251042 2316 42-43 0.6736659160262732 2316 44-45 0.5772723380397196 2316 46-47 0.5937370575028851 2316 48-49 0.6198896735743702 2316 50-51 0.37291483005247983 2316 52-53 0.1304736915726039 2316 54-55 0.06058195774449615 2316 56-57 0.2483809134226007 2316 58-59 0.3117605439595721 2316 60-61 0.3759490864078998 2316 62-63 0.3322398863908873 2316 64-65 0.33465161139415045 2316 66-67 0.393956756940959 2316 68-69 0.37206847369248663 2316 70-71 0.27014244922987274 2316 72-73 0.4182791841829072 2316 74-75 0.512142762768903 2316 76-77 0.5517006426414355 2316 78-79 0.6157986445688124 2316 80-81 0.6878604974164659 2316 82-83 0.7137549096393876 2316 84-85 0.7849169543032133 2316 86-87 0.8216246836529209 2316 88-89 0.8777503789884555 2316 90-91 0.9453111399929774 2316 92-93 0.9703312659681345 2316 94-95 1.0630916578140308 2316 96-97 1.1020272623536798 2316 98-99 1.248680507557328 2316 100 1.1795837054358138 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 28.0 1 500.0 2 257.0 3 172.0 4 142.0 5 138.0 6 166.0 7 855.0 8 3167.0 9 6193.0 10 9276.0 11 12801.0 12 16459.0 13 20077.0 14 23536.0 15 27482.0 16 33782.0 17 42168.0 18 51888.0 19 61150.0 20 71545.0 21 84922.0 22 92500.0 23 95220.0 24 96742.0 25 100335.0 26 110332.0 27 125553.0 28 148381.0 29 173690.0 30 207730.0 31 253948.0 32 318829.0 33 409476.0 34 565144.0 35 751605.0 36 1085527.0 37 1596890.0 38 1564855.0 39 425375.0 40 683.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.56510779581661 18.38492258695025 12.34397912503232 23.705990492200822 2 15.936039817014105 20.416864680635143 41.14054413538979 22.506551366960963 3 18.14757370959089 27.661386364824676 30.377978284825147 23.81306164075929 4 12.00835021238847 16.99639921253589 38.84510465479974 32.1501459202759 5 12.381706886480535 37.742571919138655 34.33210156950902 15.543619624871784 6 28.79749154106841 37.10907982596506 20.319572710235043 13.773855922731487 7 26.697960612497734 33.12818499579751 23.2941702745569 16.87968411714785 8 26.229737921497126 34.80729097018017 21.259381163541516 17.703589944781193 9 28.012973015280306 15.721651345671969 20.610338765467066 35.655036873580656 10-11 24.829507736365088 25.73062726093011 29.41325899071049 20.02660601199431 12-13 25.316187149108814 24.12023223881897 28.721005816918765 21.84257479515345 14-15 22.973710430526147 25.511392115720056 26.969007457649457 24.54588999610434 16-17 22.033052612261645 27.604023296076193 27.591994721866293 22.770929369795866 18-19 21.570626954891086 27.20863720755487 29.41352641552265 21.80720942203139 20-21 22.89966591403196 26.78379665587981 28.7683243649863 21.548213065101933 22-23 22.368676478106156 26.650959792708388 28.799184565434423 22.18117916375103 24-25 21.850094240291433 26.353750782230517 29.27275443655997 22.52340054091808 26-27 21.913642961235283 26.563342216053286 28.938766289757545 22.584248532953882 28-29 21.739712144341087 27.570795844213247 28.843997072385907 21.84549493905976 30-31 23.043538025609276 26.636070950684044 28.89350591402222 21.42688510968446 32-33 22.67376312645444 26.76911385220465 28.679694103603044 21.877428917737866 34-35 22.738982219500098 26.997571805484284 28.805346115392428 21.45809985962319 36-37 22.342797160793538 26.955785669793247 28.76567906039235 21.93573810902086 38-39 22.316996357376944 27.240499642910915 28.6407220361053 21.80178196360684 40-41 22.51231107759426 26.530197540858758 28.99514380857136 21.962347572975624 42-43 22.152341345792717 26.861625965795316 28.404272354132807 22.58176033427916 44-45 21.5658411151712 27.161234695757685 28.917537792257132 22.355386396813984 46-47 21.506845549594914 26.56058800001584 28.647884859936045 23.2846815904532 48-49 21.83175908390266 26.779899029151544 28.746689283478283 22.641652603467517 50-51 21.744727563946732 27.11942470372816 28.610174449698523 22.525673282626585 52-53 21.618161918338153 27.313518034532446 28.171395699581737 22.896924347547667 54-55 21.40338427111579 26.891455859463925 28.758413609156396 22.94674626026389 56-57 21.82086556624596 26.920557076044943 28.35341870177875 22.905158655930347 58-59 21.50917393284204 27.08535336007917 28.62957056500258 22.775902142076205 60-61 22.16441290686804 27.109450000628826 28.220265718676185 22.505871373826945 62-63 21.98140141727554 26.921294778662634 28.308951068674386 22.78835273538744 64-65 21.651536305123674 26.50267681456912 28.431697471800955 23.414089408506253 66-67 21.58122007181716 26.53608920476368 28.21904908530599 23.66364163811317 68-69 21.274704959008595 26.764574210900587 28.40354913343537 23.557171696655445 70-71 21.517133216760595 26.44041259691368 28.600991456437708 23.441462729888016 72-73 21.50468045158758 26.79844182950507 28.237152198245692 23.45972552066166 74-75 21.762695916642578 26.80659911969427 28.031690773325785 23.399014190337365 76-77 21.600614538953433 26.4974219837058 28.11850485243688 23.783458624903883 78-79 21.512446964128465 26.540342195084676 28.428723427481863 23.518487413305 80-81 21.780662754330113 26.544815746508355 28.18375731729087 23.490764181870667 82-83 22.195841996713504 26.520947214863007 28.293505073200905 22.989705715222584 84-85 22.262830091434367 26.777629252476316 27.76328910583506 23.19625155025426 86-87 21.88198039759972 26.86829555806852 28.102556770019245 23.147167274312512 88-89 21.98140075180423 26.230147298388403 28.185904916630122 23.602547033177245 90-91 22.684944695362503 26.030435977934737 28.178681913417375 23.105937413285385 92-93 22.670346086473934 26.178033241935882 28.07124948778885 23.08037118380133 94-95 22.456939846236963 26.005327900899854 28.118706465825156 23.419025787038024 96-97 22.434145869698515 25.99031635939271 28.09887241954594 23.476665351362836 98-99 22.455098885806034 25.997437965278607 27.816797049001824 23.73066609991353 100 22.675450655742228 25.84706370232073 27.80723902341997 23.670246618517066 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1636.0 1 1206.5 2 2544.0 3 3924.0 4 2641.5 5 1894.5 6 2522.0 7 4423.0 8 6979.5 9 8213.0 10 7429.0 11 6550.0 12 6930.0 13 7555.0 14 8290.0 15 9227.0 16 10049.0 17 10850.0 18 11973.0 19 13208.0 20 14498.0 21 16414.5 22 19070.0 23 22896.5 24 27862.5 25 34318.5 26 42990.5 27 53590.5 28 65799.0 29 80440.0 30 97687.5 31 113949.5 32 132914.0 33 151945.0 34 168949.5 35 186889.5 36 206966.5 37 231183.5 38 244799.5 39 246043.5 40 248487.0 41 253149.5 42 258782.0 43 268115.0 44 281254.5 45 299477.5 46 319480.0 47 335509.0 48 355408.5 49 372380.0 50 376210.5 51 371026.0 52 353787.5 53 331948.0 54 309312.0 55 278507.5 56 247476.0 57 217672.5 58 185532.5 59 154791.5 60 121717.0 61 91562.5 62 70400.0 63 53951.0 64 39585.5 65 27947.5 66 20114.5 67 15165.5 68 11374.5 69 8329.0 70 6299.0 71 4787.5 72 3625.5 73 2714.0 74 2036.5 75 1417.5 76 989.5 77 688.5 78 496.0 79 369.5 80 269.5 81 211.0 82 149.5 83 101.0 84 83.5 85 70.5 86 60.0 87 54.5 88 49.5 89 37.0 90 21.5 91 21.0 92 19.5 93 16.0 94 13.5 95 10.5 96 8.0 97 5.0 98 3.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21969798308845934 2 0.14765669649255098 3 0.02407585337432748 4 0.022737012398482383 5 0.027649976675061784 6 0.05595191069488291 7 0.061982516133906915 8 0.05545130059087127 9 0.04304082684955933 10-11 0.045002519931558446 12-13 0.043099037326769986 14-15 0.02048426693042998 16-17 0.01903482604788464 18-19 0.018679742136899633 20-21 0.022224760199028606 22-23 0.025566041590920286 24-25 0.022085055053723032 26-27 0.01960528872454907 28-29 0.019075573381932096 30-31 0.017434037924591587 32-33 0.025990978074558076 34-35 0.02647994608312759 36-37 0.025496189018267498 38-39 0.023586885365757964 40-41 0.02480930538718175 42-43 0.03124738416668035 44-45 0.0407589761429016 46-47 0.045881498137439365 48-49 0.041626312253340375 50-51 0.02829611297210007 52-53 0.04419339429833032 54-55 0.042522753602384486 56-57 0.044624151829689186 58-59 0.048122601510049635 60-61 0.024343621569496498 62-63 0.025589325781804544 64-65 0.026311135699216684 66-67 0.03158500493450215 68-69 0.03591586443897499 70-71 0.04032239756382168 72-73 0.04434474153907803 74-75 0.02516438929816675 76-77 0.01883108937764734 78-79 0.018411973941730615 80-81 0.021095476941141875 82-83 0.019174531193190212 84-85 0.011741053253389392 86-87 0.0137725989080413 88-89 0.01915124700230595 90-91 0.020047688351350058 92-93 0.020396951214613997 94-95 0.024267947949122647 96-97 0.02502468415286118 98-99 0.030199595576888533 100 0.02947778565947639 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8589519.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.29178511084304 #Duplication Level Percentage of deduplicated Percentage of total 1 79.3685560769367 51.82114707933246 2 13.29658026343804 17.363149225389467 3 3.63161507090459 7.1134389244440435 4 1.3864305129336214 3.6209009248631157 5 0.6829161603893905 2.229440759643306 6 0.398145152835237 1.5597364657105233 7 0.2639789706054349 1.2064960755786125 8 0.17329720243526525 0.9051906961370884 9 0.12655510899972885 0.7436708083340653 >10 0.6200494410127955 7.127526204619565 >50 0.03306244287344773 1.4733491296687395 >100 0.016224831692590397 1.9921496328945836 >500 0.0015533502988970768 0.6647840702617247 >1k 9.491300923428066E-4 1.1165516367392851 >5k 2.15711379814635E-5 0.10170187141130149 >10k+ 6.47134139443905E-5 0.9607664949720734 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29976 0.3489834529733271 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22426 0.261085632385236 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15814 0.184108097321864 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1642095442131276E-5 2 0.0 1.1642095442131276E-5 0.0 0.0 1.1642095442131276E-5 3 0.0 1.1642095442131276E-5 0.0 0.0 1.1642095442131276E-5 4 0.0 1.1642095442131276E-5 0.0 0.0 1.1642095442131276E-5 5 0.0 1.1642095442131276E-5 0.0 0.0 1.1642095442131276E-5 6 0.0 1.1642095442131276E-5 0.0 0.0 1.1642095442131276E-5 7 0.0 1.1642095442131276E-5 0.0 0.0 1.1642095442131276E-5 8 0.0 1.1642095442131276E-5 0.0 0.0 1.1642095442131276E-5 9 0.0 1.1642095442131276E-5 0.0 2.328419088426255E-5 1.1642095442131276E-5 10-11 0.0 1.1642095442131276E-5 0.0 6.403152493172202E-5 1.1642095442131276E-5 12-13 0.0 1.7463143163196913E-5 0.0 1.222420021423784E-4 1.1642095442131276E-5 14-15 0.0 2.910523860532819E-5 0.0 1.804524793530348E-4 2.3284190884262555E-5 16-17 0.0 3.492628632639383E-5 0.0 2.3866295656369116E-4 3.492628632639383E-5 18-19 0.0 3.492628632639383E-5 0.0 3.4926286326393834E-4 4.65683817685251E-5 20-21 0.0 3.492628632639383E-5 0.0 6.927046788068109E-4 5.821047721065638E-5 22-23 0.0 3.492628632639383E-5 0.0 0.0018278089844146104 5.821047721065638E-5 24-25 0.0 3.492628632639383E-5 0.0 0.004447280458894148 8.149466809491894E-5 26-27 0.0 3.492628632639383E-5 0.0 0.008522013863640095 9.31367635370502E-5 28-29 0.0 3.492628632639383E-5 0.0 0.020303814451076947 9.31367635370502E-5 30-31 0.0 4.074733404745947E-5 0.0 0.06212222127921249 9.31367635370502E-5 32-33 0.0 6.403152493172202E-5 0.0 0.15524152167309951 1.1642095442131276E-4 34-35 1.1642095442131276E-5 8.149466809491894E-5 0.0 0.27870012278918066 1.513472407477066E-4 36-37 1.1642095442131276E-5 9.313676353705022E-5 0.0 0.4656430703512036 1.5716828846877224E-4 38-39 1.1642095442131276E-5 1.3388409758450967E-4 0.0 0.7960864863329367 1.6298933618983787E-4 40-41 1.1642095442131276E-5 1.688103839109035E-4 0.0 1.2126814085864412 1.6298933618983787E-4 42-43 1.1642095442131276E-5 1.8627352707410044E-4 0.0 1.5966260741724887 1.6298933618983787E-4 44-45 1.1642095442131276E-5 1.979156225162317E-4 0.0 1.997684620058469 1.6298933618983787E-4 46-47 1.1642095442131276E-5 2.2119981340049425E-4 0.0 2.4512839426747877 1.862735270741004E-4 48-49 1.1642095442131276E-5 2.2119981340049425E-4 0.0 2.9419924445129 2.0955771795836298E-4 50-51 1.1642095442131276E-5 2.2119981340049425E-4 0.0 3.4837631769602 2.3284190884262552E-4 52-53 1.1642095442131276E-5 2.2119981340049425E-4 0.0 4.0408258017707395 2.5030505200582243E-4 54-55 1.1642095442131276E-5 2.2119981340049425E-4 0.0 4.619350629528848 2.6776819516901933E-4 56-57 1.1642095442131276E-5 2.2119981340049425E-4 0.0 5.22378494069342 2.794102906111506E-4 58-59 1.1642095442131276E-5 2.270208611215599E-4 0.0 5.907822079443564 2.9105238605328193E-4 60-61 1.1642095442131276E-5 2.3866295656369116E-4 0.0 6.581131027243784 3.026944814954132E-4 62-63 1.1642095442131276E-5 2.444840042847568E-4 0.0 7.325398546763794 3.2597867237967574E-4 64-65 1.1642095442131276E-5 2.444840042847568E-4 0.0 7.985237590137469 3.2597867237967574E-4 66-67 1.1642095442131276E-5 2.444840042847568E-4 0.0 8.690044227156374 3.4344181554287265E-4 68-69 1.1642095442131276E-5 2.444840042847568E-4 0.0 9.459965103983123 3.6090495870606955E-4 70-71 1.1642095442131276E-5 2.444840042847568E-4 0.0 10.26441643589123 3.783681018692665E-4 72-73 1.1642095442131276E-5 2.444840042847568E-4 0.0 10.998939521526175 4.074733404745947E-4 74-75 1.1642095442131276E-5 2.444840042847568E-4 0.0 11.709212122355163 4.423996268009885E-4 76-77 1.1642095442131276E-5 2.5612609972688806E-4 0.0 12.466646851820224 4.4822067452205414E-4 78-79 1.1642095442131276E-5 2.619471474479537E-4 0.0 13.288701032036835 4.6568381768525105E-4 80-81 1.1642095442131276E-5 2.6776819516901933E-4 0.0 14.073244380738899 4.6568381768525105E-4 82-83 1.1642095442131276E-5 2.9105238605328193E-4 0.0 14.864080282027434 4.6568381768525105E-4 84-85 1.1642095442131276E-5 2.9105238605328193E-4 0.0 15.637720808347941 4.6568381768525105E-4 86-87 1.1642095442131276E-5 2.9105238605328193E-4 5.821047721065638E-6 16.43080945510453 4.715048654063167E-4 88 1.1642095442131276E-5 2.9105238605328193E-4 1.1642095442131276E-5 17.036786343915182 4.773259131273823E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 11550 0.0 59.207813 2 ACCTGGG 13345 0.0 50.266285 3 GGTATCA 16265 0.0 43.820316 1 GTACCTG 16950 0.0 41.076683 1 CCTGGGG 11795 0.0 38.9375 4 GTATCAA 27580 0.0 37.474255 1 TAACGCA 1120 0.0 36.515114 4 ATAACGC 1145 0.0 34.48619 3 TATCACG 810 0.0 31.957516 2 CTGGGGG 8370 0.0 31.507128 5 GTACATG 29145 0.0 31.162596 1 ATCAACG 33155 0.0 30.596647 3 TACATGG 29310 0.0 30.509363 2 CAACGCA 33290 0.0 30.401964 5 TATCAAC 34040 0.0 29.96158 2 TCAACGC 34115 0.0 29.749432 4 AACGCAG 35305 0.0 29.053268 6 ACATGGG 29830 0.0 28.822515 3 TGGGGGG 19660 0.0 28.812437 6 CATGGGG 22315 0.0 28.122622 4 >>END_MODULE