FastQCFastQC Report
Mon 6 Jun 2016
ERR1211433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211433_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8589519
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274910.3200528457996309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219570.25562548962287646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158920.18501618076635024No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT89770.10451109078401247No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG114250.059.5190162
ACCTGGG130350.050.618833
GGTATCA163650.042.7781371
CCTGGGG107950.040.6621554
TAACGCA12300.040.1190264
ATAACGC12150.039.8397873
GTACCTG174550.039.7027741
GTATCAA271800.036.567791
CTGGGGG74700.035.101175
TGGGGGG176400.030.5788366
ATCAACG337600.028.968483
GTACATG292700.028.8648011
CAACGCA339850.028.8426425
TATCAAC347650.028.305962
TCAACGC347800.028.2682084
TACATGG295750.028.0296212
AACGCAG364050.027.465556
ACATGGG292550.026.9555323
CATGGGG215000.026.7552594
TATAACG19600.026.6140082
Produced by FastQC (version 0.11.5)