##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211433_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8589519 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06232968341999 33.0 31.0 34.0 30.0 34.0 2 32.194422877462635 33.0 31.0 34.0 30.0 34.0 3 32.27370496531878 34.0 31.0 34.0 30.0 34.0 4 35.92328091945544 37.0 35.0 37.0 35.0 37.0 5 35.78861750000204 37.0 35.0 37.0 35.0 37.0 6 35.822903936762934 37.0 35.0 37.0 35.0 37.0 7 35.75383394576576 37.0 35.0 37.0 33.0 37.0 8 35.73257594517225 37.0 35.0 37.0 33.0 37.0 9 37.317913028657365 39.0 37.0 39.0 34.0 39.0 10-11 37.29000704230353 39.0 37.0 39.0 34.0 39.0 12-13 37.20172701172207 39.0 37.0 39.0 33.0 39.0 14-15 38.40369035798163 40.0 38.0 41.0 33.5 41.0 16-17 38.39417766000634 40.0 38.0 41.0 33.5 41.0 18-19 38.40726168717946 40.0 38.0 41.0 33.5 41.0 20-21 38.368211654226506 40.0 38.0 41.0 34.0 41.0 22-23 38.26311671235607 40.0 38.0 41.0 34.0 41.0 24-25 38.15091904447735 40.0 37.5 41.0 33.0 41.0 26-27 38.02878828255692 40.0 37.0 41.0 33.0 41.0 28-29 37.95058035263674 40.0 37.0 41.0 33.0 41.0 30-31 37.98758958446916 40.0 37.0 41.0 33.0 41.0 32-33 37.81759304566414 40.0 37.0 41.0 33.0 41.0 34-35 37.70894580942193 40.0 37.0 41.0 33.0 41.0 36-37 37.568183736481636 40.0 37.0 41.0 32.5 41.0 38-39 37.45454105171663 40.0 36.0 41.0 32.0 41.0 40-41 37.399457001026484 40.0 36.0 41.0 32.0 41.0 42-43 37.26784805994375 40.0 36.0 41.0 31.0 41.0 44-45 37.125389209803245 40.0 36.0 41.0 31.0 41.0 46-47 36.914602202987155 39.0 36.0 41.0 31.0 41.0 48-49 36.71139198830575 39.0 35.0 41.0 30.0 41.0 50-51 36.538173790639505 39.0 35.0 41.0 30.0 41.0 52-53 36.348401581043134 39.0 35.0 40.0 29.5 41.0 54-55 36.155397234699635 39.0 35.0 40.0 29.0 41.0 56-57 35.94286996745685 38.0 35.0 40.0 28.5 41.0 58-59 35.69784967004555 38.0 34.5 40.0 28.0 41.0 60-61 35.43725999092615 38.0 34.0 40.0 28.0 41.0 62-63 35.140434347953594 37.0 34.0 40.0 27.0 41.0 64-65 34.797221183165206 37.0 34.0 40.0 26.5 41.0 66-67 34.44915314815649 36.0 33.0 39.0 26.0 41.0 68-69 34.08766614288879 36.0 33.0 39.0 26.0 40.0 70-71 33.7125987497088 35.0 33.0 38.5 26.0 40.0 72-73 33.35181475237438 35.0 32.0 38.0 25.0 40.0 74-75 32.97619721197427 35.0 32.0 37.0 25.0 39.0 76-77 31.961481312283027 34.0 30.5 36.0 23.5 39.0 78-79 32.4480213036376 35.0 32.0 36.0 25.0 38.5 80-81 32.33872641762595 35.0 32.0 36.0 25.0 37.5 82-83 32.05816757608895 34.5 31.5 35.0 24.5 37.0 84-85 31.773615437604832 34.0 31.0 35.0 24.0 37.0 86-87 31.52456540348767 34.0 31.0 35.0 23.5 36.0 88-89 31.305246021342988 34.0 31.0 35.0 23.0 36.0 90-91 31.0874952369277 34.0 31.0 35.0 22.0 35.5 92-93 30.906928665039334 34.0 30.5 35.0 20.5 35.0 94-95 30.73766691708814 34.0 30.0 35.0 20.0 35.0 96-97 30.56953258965956 34.0 30.0 35.0 20.0 35.0 98-99 30.38855889369358 34.0 29.5 35.0 19.0 35.0 100 28.58718433476892 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.25253402728501584 1101 2 -0.2632086316476574 1101 3 -0.27805882338702403 1101 4 -0.13054246947146453 1101 5 -0.1494803159727951 1101 6 -0.1923607805445613 1101 7 -0.15536717610003592 1101 8 -0.3312849085309182 1101 9 -0.40071090525766095 1101 10-11 -0.38032097930624076 1101 12-13 -0.41261046206579266 1101 14-15 -0.5479740024637891 1101 16-17 -0.5939422625928543 1101 18-19 -0.5789096165414591 1101 20-21 -0.6398894821103553 1101 22-23 -0.6226037166329021 1101 24-25 -0.6963330033636197 1101 26-27 -0.6953074416721776 1101 28-29 -0.7275175837979546 1101 30-31 -0.6456338155099388 1101 32-33 -0.5039399950847425 1101 34-35 -0.5112917484367046 1101 36-37 -0.6144674156919692 1101 38-39 -0.6353793813240287 1101 40-41 -0.6705017915657834 1101 42-43 -0.6358706056048149 1101 44-45 -0.6362741794758904 1101 46-47 -0.7772823304735894 1101 48-49 -0.7182935323762081 1101 50-51 -0.8591503742643312 1101 52-53 -0.8346723820123358 1101 54-55 -0.7586533896478898 1101 56-57 -0.7696903565435633 1101 58-59 -0.768660313119625 1101 60-61 -0.776530555564328 1101 62-63 -0.8845368136826934 1101 64-65 -0.8744190585245448 1101 66-67 -0.896252506731372 1101 68-69 -0.9078804273554653 1101 70-71 -0.8964211093725325 1101 72-73 -1.022112620743897 1101 74-75 -1.0981987107322233 1101 76-77 -0.8748178309419039 1101 78-79 -0.7623779750722193 1101 80-81 -0.6327176135842194 1101 82-83 -0.7297268480453383 1101 84-85 -0.7169294444677057 1101 86-87 -0.7616526672426112 1101 88-89 -0.8137513645946299 1101 90-91 -0.9582670305599699 1101 92-93 -1.085060942648692 1101 94-95 -1.144433230996544 1101 96-97 -1.121882308639229 1101 98-99 -1.211195523919912 1101 100 -1.0322160710205814 1102 1 -0.2993335813489395 1102 2 -0.2523566290751482 1102 3 -0.35064412662167754 1102 4 -0.21216887955547747 1102 5 -0.258630475909321 1102 6 -0.3901131691811841 1102 7 -0.3285736069295311 1102 8 -0.3659660896003274 1102 9 -0.49584263491982483 1102 10-11 -0.48387470138922595 1102 12-13 -0.4226835222394314 1102 14-15 -0.6243762788811935 1102 16-17 -0.6506311507339362 1102 18-19 -0.7644726532686192 1102 20-21 -0.6888438724790333 1102 22-23 -0.6939862105663437 1102 24-25 -0.7825132071427632 1102 26-27 -0.8043763677167064 1102 28-29 -0.8541107771313605 1102 30-31 -0.7477514213959893 1102 32-33 -0.7032505481255455 1102 34-35 -0.7372880873029857 1102 36-37 -0.6754006883408366 1102 38-39 -0.7717024343841459 1102 40-41 -0.8242261344273487 1102 42-43 -0.8075063144118886 1102 44-45 -0.7456905987484319 1102 46-47 -0.8418123533457234 1102 48-49 -0.8376654866603559 1102 50-51 -0.992417526400132 1102 52-53 -0.9575310120467435 1102 54-55 -0.889610869305784 1102 56-57 -0.9288960709884932 1102 58-59 -0.9554008054236576 1102 60-61 -1.0224316979802879 1102 62-63 -1.0538820359472751 1102 64-65 -1.038110556512052 1102 66-67 -1.0058497573514629 1102 68-69 -1.0251746509201496 1102 70-71 -1.0184081074761124 1102 72-73 -1.0722837760641823 1102 74-75 -1.1199747556518815 1102 76-77 -0.8624090126550676 1102 78-79 -0.8303728972878517 1102 80-81 -0.7424957942364863 1102 82-83 -0.7794739637651915 1102 84-85 -0.8854330184793504 1102 86-87 -0.884876389262768 1102 88-89 -0.7804213733805234 1102 90-91 -0.7864871681901526 1102 92-93 -0.8819059985474418 1102 94-95 -0.873698752928167 1102 96-97 -0.956027438127105 1102 98-99 -1.0334609820683767 1102 100 -1.0067826588481026 1103 1 -0.33630773124935587 1103 2 -0.30063848232418167 1103 3 -0.32257901892527485 1103 4 -0.18801958225136417 1103 5 -0.1522002315591422 1103 6 -0.27400326199614966 1103 7 -0.2958644559066883 1103 8 -0.2953241272863565 1103 9 -0.35555746027579005 1103 10-11 -0.4079742441509637 1103 12-13 -0.5007587208953268 1103 14-15 -0.7158341055921014 1103 16-17 -0.7955649183387763 1103 18-19 -0.7389235095672859 1103 20-21 -0.6906339835977775 1103 22-23 -0.7682816507074435 1103 24-25 -0.737740012487464 1103 26-27 -0.8172352211392351 1103 28-29 -0.755880139650678 1103 30-31 -0.7111363768885042 1103 32-33 -0.654994405977483 1103 34-35 -0.6840742765883334 1103 36-37 -0.6454983621905015 1103 38-39 -0.7033273881086259 1103 40-41 -0.7973464898234113 1103 42-43 -0.7614334381743291 1103 44-45 -0.7659011836751688 1103 46-47 -0.7860501483241435 1103 48-49 -0.7895778995605554 1103 50-51 -0.7522625182524791 1103 52-53 -0.8437040660519557 1103 54-55 -0.8329568226124451 1103 56-57 -0.8368765425329983 1103 58-59 -0.9558838843053366 1103 60-61 -0.936984383515302 1103 62-63 -0.9452246565253404 1103 64-65 -0.8960996375514796 1103 66-67 -0.9734278485466987 1103 68-69 -0.9018887965024547 1103 70-71 -0.8450612822911197 1103 72-73 -0.9730112804092101 1103 74-75 -0.9399919649272128 1103 76-77 -0.898016831258392 1103 78-79 -0.7606583965588172 1103 80-81 -0.7022850328292698 1103 82-83 -0.635337976975002 1103 84-85 -0.6984102109679817 1103 86-87 -0.7569798689963712 1103 88-89 -0.6722648908922864 1103 90-91 -0.7996589506454193 1103 92-93 -0.8952291899200411 1103 94-95 -0.9074454229722484 1103 96-97 -0.9557059036471536 1103 98-99 -0.9699388251466274 1103 100 -0.8980782449498896 1104 1 -0.37286383918916144 1104 2 -0.3613792062418355 1104 3 -0.39194699653827314 1104 4 -0.20977462613211628 1104 5 -0.25048521161824766 1104 6 -0.37092300677490897 1104 7 -0.356475137965532 1104 8 -0.3350210590075946 1104 9 -0.5774694726372047 1104 10-11 -0.4948533163327795 1104 12-13 -0.5898199441580445 1104 14-15 -0.6699142240561642 1104 16-17 -0.7008090495034409 1104 18-19 -0.7108343782008291 1104 20-21 -0.7106830920047216 1104 22-23 -0.775334971464126 1104 24-25 -0.8211075460072621 1104 26-27 -0.805652713509339 1104 28-29 -0.8636294106467091 1104 30-31 -0.8293493527426108 1104 32-33 -0.7670809381406585 1104 34-35 -0.8356926760326786 1104 36-37 -0.7831739346303905 1104 38-39 -0.9212593091776782 1104 40-41 -0.8694840126428218 1104 42-43 -0.7653475445380806 1104 44-45 -0.8946483098546594 1104 46-47 -0.844339789891265 1104 48-49 -0.8574973458263457 1104 50-51 -0.9054013405101387 1104 52-53 -0.7736700630223865 1104 54-55 -0.7744998209800187 1104 56-57 -0.8093064348738892 1104 58-59 -0.8709535365156569 1104 60-61 -0.9581847683960731 1104 62-63 -0.9519807714255464 1104 64-65 -0.8912961520571443 1104 66-67 -0.8456212261335878 1104 68-69 -0.7755047509833943 1104 70-71 -0.763079187187536 1104 72-73 -0.7907933731273005 1104 74-75 -0.7702548818221473 1104 76-77 -0.7707853650825918 1104 78-79 -0.6729680291819875 1104 80-81 -0.6956524242000413 1104 82-83 -0.6922365105034629 1104 84-85 -0.6328660587423762 1104 86-87 -0.6377799540243707 1104 88-89 -0.7090103184724974 1104 90-91 -0.6815342297354441 1104 92-93 -0.8191500949290287 1104 94-95 -0.9192335401952043 1104 96-97 -0.8737503838775709 1104 98-99 -0.8662457687021856 1104 100 -0.9903454191267365 1105 1 -0.4339977164511879 1105 2 -0.39370265425733564 1105 3 -0.4089944417944622 1105 4 -0.26192076081919424 1105 5 -0.28070578364360443 1105 6 -0.3812478096398735 1105 7 -0.3605681816680786 1105 8 -0.32478650763060557 1105 9 -0.45717386816864547 1105 10-11 -0.5305277547677818 1105 12-13 -0.5207387624060331 1105 14-15 -0.7096926408224533 1105 16-17 -0.6979869034268589 1105 18-19 -0.681011614497244 1105 20-21 -0.7141796109085377 1105 22-23 -0.7377158501082235 1105 24-25 -0.751230355923532 1105 26-27 -0.7410588422675559 1105 28-29 -0.6751811539300903 1105 30-31 -0.7568898439992466 1105 32-33 -0.6505852314023102 1105 34-35 -0.6879917747516231 1105 36-37 -0.7854768164044614 1105 38-39 -0.7320779590454478 1105 40-41 -0.7343971848360766 1105 42-43 -0.7713448130351779 1105 44-45 -0.7850157146860113 1105 46-47 -0.9306912385206729 1105 48-49 -0.8190196113090735 1105 50-51 -0.7455748498933872 1105 52-53 -0.7967953666215379 1105 54-55 -0.8677338676667716 1105 56-57 -0.83612109853091 1105 58-59 -0.8750326916076716 1105 60-61 -1.0599361401370828 1105 62-63 -0.9301937195013394 1105 64-65 -0.9208169404836966 1105 66-67 -0.9916436129417363 1105 68-69 -1.0383415481568719 1105 70-71 -0.9086369991572312 1105 72-73 -0.9527307530218323 1105 74-75 -1.0005875321600755 1105 76-77 -0.971064628972524 1105 78-79 -0.8163782637464223 1105 80-81 -0.737810460985699 1105 82-83 -0.7376341350581903 1105 84-85 -0.6947790527827742 1105 86-87 -0.723104771577642 1105 88-89 -0.8075972086555971 1105 90-91 -0.7832128453944698 1105 92-93 -0.8556037807051418 1105 94-95 -0.790970736740487 1105 96-97 -0.8525368287503632 1105 98-99 -0.8396302132103166 1105 100 -0.9263413487030299 1106 1 -0.40302662261884237 1106 2 -0.37543601674082083 1106 3 -0.3764919153807398 1106 4 -0.22886607139649584 1106 5 -0.27281351970783874 1106 6 -0.3305305193268069 1106 7 -0.35636419234202066 1106 8 -0.40669548981934867 1106 9 -0.5051230283344594 1106 10-11 -0.49835043236113563 1106 12-13 -0.5738822495382223 1106 14-15 -0.7297273385792877 1106 16-17 -0.7344266246616584 1106 18-19 -0.7908431057075731 1106 20-21 -0.7278324066357058 1106 22-23 -0.7569110921620918 1106 24-25 -0.8638930302829735 1106 26-27 -0.7554065727188899 1106 28-29 -0.8012805740587439 1106 30-31 -0.7952564904147437 1106 32-33 -0.7280857671540488 1106 34-35 -0.7574263446913392 1106 36-37 -0.7661246813073674 1106 38-39 -0.8149408782890646 1106 40-41 -0.8391747195333537 1106 42-43 -0.7207183413081495 1106 44-45 -0.8777529248497373 1106 46-47 -0.8701162911941864 1106 48-49 -0.936416444989284 1106 50-51 -0.9357759395128724 1106 52-53 -0.9244628584529551 1106 54-55 -0.9440878724404129 1106 56-57 -1.0210127119314407 1106 58-59 -1.0063918134544352 1106 60-61 -1.1307743032932578 1106 62-63 -0.9366396763838338 1106 64-65 -1.0844081366880332 1106 66-67 -1.0361515740312441 1106 68-69 -1.0357605256060651 1106 70-71 -0.9688750383128664 1106 72-73 -0.985081284330434 1106 74-75 -0.953935307837817 1106 76-77 -0.9803359169222396 1106 78-79 -0.818049017222954 1106 80-81 -0.7408790033039807 1106 82-83 -0.8793415982627479 1106 84-85 -0.8381943856571432 1106 86-87 -0.8029012635217079 1106 88-89 -0.8263974351820522 1106 90-91 -0.9086935986922597 1106 92-93 -0.9114573065304121 1106 94-95 -0.868773497536452 1106 96-97 -0.9463850280361434 1106 98-99 -0.9455971676488701 1106 100 -1.0752035863063298 1107 1 -0.41944228405396444 1107 2 -0.3619730650519237 1107 3 -0.42447931098669756 1107 4 -0.26768330353839787 1107 5 -0.265472699152717 1107 6 -0.32497639736669015 1107 7 -0.38064440322088444 1107 8 -0.4532417039313259 1107 9 -0.5936045264826504 1107 10-11 -0.5556889298372667 1107 12-13 -0.6088463879605825 1107 14-15 -0.718872397521146 1107 16-17 -0.6845047477359927 1107 18-19 -0.7572454939636586 1107 20-21 -0.7869615290375336 1107 22-23 -0.7294297765882831 1107 24-25 -0.7887137223947391 1107 26-27 -0.8071494491007698 1107 28-29 -0.763002994257711 1107 30-31 -0.8309782470638112 1107 32-33 -0.883461906362534 1107 34-35 -0.7993164213922128 1107 36-37 -0.8596153594348479 1107 38-39 -0.8585768633716242 1107 40-41 -0.882116780198217 1107 42-43 -0.8954142088232757 1107 44-45 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0.9485076578680136 2115 54-55 0.9646253456354472 2115 56-57 0.834045086798092 2115 58-59 0.7755344128712238 2115 60-61 0.8669311140010834 2115 62-63 0.9041087347319987 2115 64-65 0.8687073954895865 2115 66-67 0.9996266226274741 2115 68-69 1.0174355974803504 2115 70-71 1.0420612018255113 2115 72-73 0.9950856772595245 2115 74-75 0.7172194084001049 2115 76-77 0.6687519709240242 2115 78-79 0.5498030786436132 2115 80-81 0.6661456229923957 2115 82-83 0.7740469818887945 2115 84-85 0.7855975051190391 2115 86-87 0.7745887511319118 2115 88-89 0.8680924455892693 2115 90-91 0.9146822133526804 2115 92-93 0.912290126973037 2115 94-95 0.9227496932720776 2115 96-97 0.9722856107403572 2115 98-99 0.9697906011618436 2115 100 0.9870861601401053 2116 1 0.1549625753384234 2116 2 0.2666355462288479 2116 3 0.3231451167114159 2116 4 0.20189098761637325 2116 5 0.29752742011024225 2116 6 0.27551365034121744 2116 7 0.3474500032275287 2116 8 0.39900083706442047 2116 9 0.4881334202895218 2116 10-11 0.5261691959674977 2116 12-13 0.40814745089567595 2116 14-15 0.5844875881247233 2116 16-17 0.7245195203324784 2116 18-19 0.5816271234665962 2116 20-21 0.7315414863692737 2116 22-23 0.7876811402655761 2116 24-25 0.8191673296278523 2116 26-27 0.7975839471842008 2116 28-29 0.7620833265483569 2116 30-31 0.5897167568977082 2116 32-33 0.5861197814724974 2116 34-35 0.6084501820133426 2116 36-37 0.6395337376093764 2116 38-39 0.5876056090537318 2116 40-41 0.6965593866036457 2116 42-43 0.7226780748088473 2116 44-45 0.792289511639396 2116 46-47 0.6764376497293867 2116 48-49 0.5797678003514406 2116 50-51 0.7119598820735717 2116 52-53 0.718949281281752 2116 54-55 0.6997668023671721 2116 56-57 0.7695279155778891 2116 58-59 0.7711355734115202 2116 60-61 0.8149186900533394 2116 62-63 0.7714916401394802 2116 64-65 0.684701705715689 2116 66-67 0.7004299246671692 2116 68-69 0.6063362166877226 2116 70-71 0.5664174968526723 2116 72-73 0.7053504072308314 2116 74-75 0.7168673789650981 2116 76-77 0.6351272857305297 2116 78-79 0.6310898999754571 2116 80-81 0.5522899650108357 2116 82-83 0.5687492655666162 2116 84-85 0.5429722011017866 2116 86-87 0.5636625798503232 2116 88-89 0.5561876828282166 2116 90-91 0.3713133318196675 2116 92-93 0.5000451070536762 2116 94-95 0.6182484577824319 2116 96-97 0.696516162987205 2116 98-99 0.7447018535402776 2116 100 0.39003662594823396 2201 1 0.645663380250312 2201 2 0.6152727625135839 2201 3 0.5984216171584151 2201 4 0.3658332839470049 2201 5 0.47723433629268186 2201 6 0.48797343286836536 2201 7 0.517703908000108 2201 8 0.5436557637428976 2201 9 0.6691862937858417 2201 10-11 0.6399869583522957 2201 12-13 0.6582921858109714 2201 14-15 0.8112646671501906 2201 16-17 0.8160912579223449 2201 18-19 0.7838570783000094 2201 20-21 0.7713003275570429 2201 22-23 0.7389132136466472 2201 24-25 0.719228056373602 2201 26-27 0.7877676640366715 2201 28-29 0.7998567349312609 2201 30-31 0.9141770968993796 2201 32-33 0.721699540008224 2201 34-35 0.9294048256512113 2201 36-37 0.9418954037956482 2201 38-39 0.9658745154841881 2201 40-41 0.9669097400406343 2201 42-43 0.022863856649316006 2201 44-45 0.7812576151600013 2201 46-47 0.9419173920527371 2201 48-49 1.0276904441057155 2201 50-51 1.0482326035203187 2201 52-53 1.0032367453598852 2201 54-55 1.0111727547543907 2201 56-57 1.015868015900594 2201 58-59 1.02214659194388 2201 60-61 1.067229252688783 2201 62-63 1.0890974222709602 2201 64-65 1.1609764900864619 2201 66-67 1.1119083064307134 2201 68-69 1.0876464293794257 2201 70-71 1.123269557336073 2201 72-73 1.074349529946332 2201 74-75 1.0906603800696217 2201 76-77 1.101500545570513 2201 78-79 0.9440356977042583 2201 80-81 0.8385765046430791 2201 82-83 0.8634255799892614 2201 84-85 0.8181968549737881 2201 86-87 0.8210374197518355 2201 88-89 0.8167711126256947 2201 90-91 0.0522119194442503 2201 92-93 0.5341357006876706 2201 94-95 0.8105874349002811 2201 96-97 0.7730080755303739 2201 98-99 0.7626070744098854 2201 100 1.0067913874173549 2202 1 0.6736555297926117 2202 2 0.6459691326899488 2202 3 0.6263614123063164 2202 4 0.4208312781091337 2202 5 0.38354006920692285 2202 6 0.4438441893731593 2202 7 0.5305428912226589 2202 8 0.5395062592591415 2202 9 0.7364266255797887 2202 10-11 0.7142627428240047 2202 12-13 0.7417427402191521 2202 14-15 0.8833410807931301 2202 16-17 0.8756986269789522 2202 18-19 0.8557615436868176 2202 20-21 0.8372811012296424 2202 22-23 0.8532508886846983 2202 24-25 0.8425236007120702 2202 26-27 0.8801527200718127 2202 28-29 0.8636309788924805 2202 30-31 1.0595616302010171 2202 32-33 0.6877405023241394 2202 34-35 0.9952492563782158 2202 36-37 1.093097974509881 2202 38-39 1.1338430001918667 2202 40-41 1.1425300977416342 2202 42-43 1.1252127535735639 2202 44-45 1.1269647033993024 2202 46-47 1.1549706284755175 2202 48-49 1.1368479246653038 2202 50-51 1.1239109346632148 2202 52-53 1.129894911416244 2202 54-55 1.1239948538198163 2202 56-57 1.1423549360817518 2202 58-59 1.0982958965192182 2202 60-61 1.1579019310158785 2202 62-63 1.1601728080064149 2202 64-65 1.1980334869487166 2202 66-67 1.1629926610304366 2202 68-69 1.139984642009047 2202 70-71 1.1490467756104081 2202 72-73 1.194696019865134 2202 74-75 1.1258742483110638 2202 76-77 1.1731819779953874 2202 78-79 1.0082601138163412 2202 80-81 0.964691892323593 2202 82-83 0.9277751819959121 2202 84-85 0.9709750282160421 2202 86-87 0.8919952647390623 2202 88-89 0.9808072906468439 2202 90-91 1.0629599956633804 2202 92-93 1.0741997254114786 2202 94-95 1.015246570782626 2202 96-97 0.8697618599453527 2202 98-99 0.9020710226822288 2202 100 1.228360857619517 2203 1 0.6274842048569056 2203 2 0.5793788725550328 2203 3 0.5593643613870753 2203 4 0.3183858718546233 2203 5 0.4202978042042673 2203 6 0.45743745346318576 2203 7 0.4706550178172364 2203 8 0.4909714682264976 2203 9 0.6164596277916701 2203 10-11 0.6058439861050147 2203 12-13 0.6529796219923583 2203 14-15 0.7896370093101339 2203 16-17 0.7579960997378592 2203 18-19 0.7811956510903357 2203 20-21 0.831408220716888 2203 22-23 0.7691578328677835 2203 24-25 0.7603210993838658 2203 26-27 0.756631793127589 2203 28-29 0.8188235421427876 2203 30-31 0.8923710668160041 2203 32-33 0.947442856073593 2203 34-35 0.9287777909031973 2203 36-37 0.8857319462240909 2203 38-39 0.9320974581094745 2203 40-41 0.8833833883875215 2203 42-43 0.9640351499587254 2203 44-45 0.9521848229976797 2203 46-47 0.9990949460450338 2203 48-49 1.0353737778298466 2203 50-51 0.9817443621790432 2203 52-53 0.9241091148387994 2203 54-55 0.9305022315102889 2203 56-57 0.9666757758921491 2203 58-59 1.0732364978035562 2203 60-61 1.0563087068344643 2203 62-63 1.0466714279718659 2203 64-65 1.0465952163000907 2203 66-67 1.0416036414993144 2203 68-69 1.0296681139924928 2203 70-71 1.044024979683691 2203 72-73 1.045241396105041 2203 74-75 1.0832433334863154 2203 76-77 1.0820337875880348 2203 78-79 0.9267404157250567 2203 80-81 0.8567632744339377 2203 82-83 0.8568162027919044 2203 84-85 0.8405507524674327 2203 86-87 0.8065105509877988 2203 88-89 0.8686572331257736 2203 90-91 0.9221039224997973 2203 92-93 0.8991707653331247 2203 94-95 0.979120073759006 2203 96-97 0.9896586629045778 2203 98-99 0.9706890453597481 2203 100 1.1875893131309674 2204 1 0.6559101065135309 2204 2 0.6217277025322687 2204 3 0.6045721269881028 2204 4 0.38912612696199744 2204 5 0.49046519458669025 2204 6 0.50730586211656 2204 7 0.5118556581210285 2204 8 0.5079684180502397 2204 9 0.6792630903837775 2204 10-11 0.6989449292445187 2204 12-13 0.6558626310706401 2204 14-15 0.8526643244152936 2204 16-17 0.827069842823839 2204 18-19 0.8297559110101744 2204 20-21 0.8181913932553115 2204 22-23 0.8190994526533686 2204 24-25 0.839273653081996 2204 26-27 0.8485918771692766 2204 28-29 0.8556663138751759 2204 30-31 0.9506245685852264 2204 32-33 0.9659045016614698 2204 34-35 1.0194355372371149 2204 36-37 1.0116435828978254 2204 38-39 1.0822396863050088 2204 40-41 0.9943856361524084 2204 42-43 1.0242258440017125 2204 44-45 1.0454583936787643 2204 46-47 1.0241326411784684 2204 48-49 1.0790611531036092 2204 50-51 1.0206215829413026 2204 52-53 1.0286783956525625 2204 54-55 1.0383944616957095 2204 56-57 1.0580687895363141 2204 58-59 1.0578942431355784 2204 60-61 1.1073735355619974 2204 62-63 1.1267144896766652 2204 64-65 1.1393447204008496 2204 66-67 1.1250904428730593 2204 68-69 1.092538336587623 2204 70-71 1.1480276910504301 2204 72-73 1.1429812299130404 2204 74-75 1.1400019433086044 2204 76-77 1.1059292153961415 2204 78-79 1.0006613947916136 2204 80-81 0.884316909951373 2204 82-83 0.8537817730764559 2204 84-85 0.8834600736923086 2204 86-87 0.8809334490565668 2204 88-89 0.8920831589950069 2204 90-91 0.9420839323484849 2204 92-93 0.9727533022432944 2204 94-95 0.9823166889812107 2204 96-97 0.9491419369188776 2204 98-99 0.9126131917693243 2204 100 1.067874645628791 2205 1 0.6738604109761113 2205 2 0.49512501033365197 2205 3 0.5743119794556648 2205 4 0.3761423394789887 2205 5 0.5045187359671957 2205 6 0.5364368219667597 2205 7 0.5632920197756093 2205 8 0.5822098916610372 2205 9 0.7597078025935673 2205 10-11 0.7457913324973191 2205 12-13 0.7621211007916813 2205 14-15 0.9189997489574395 2205 16-17 0.9168482350640446 2205 18-19 0.9099085246054344 2205 20-21 0.9178574578114649 2205 22-23 0.8893683254558553 2205 24-25 0.9205261441764208 2205 26-27 0.9319071164580421 2205 28-29 0.9672493674377094 2205 30-31 1.0401792978280042 2205 32-33 1.0923025377436275 2205 34-35 1.0869283208585472 2205 36-37 1.082629836665994 2205 38-39 1.1105843957491786 2205 40-41 1.121525591327618 2205 42-43 1.1879104458251746 2205 44-45 1.179803258214747 2205 46-47 1.224305736653747 2205 48-49 1.1966455005439656 2205 50-51 1.2248147015285156 2205 52-53 1.2143234284577318 2205 54-55 1.2253559582557543 2205 56-57 1.2511540536994303 2205 58-59 1.286046226602565 2205 60-61 1.3017679698266562 2205 62-63 1.3195378059408611 2205 64-65 1.3029888953205457 2205 66-67 1.2580554241183393 2205 68-69 1.2945426813274352 2205 70-71 1.3181861558367203 2205 72-73 1.338819726194906 2205 74-75 1.3472490690940262 2205 76-77 1.3180675355999902 2205 78-79 1.2085654645417137 2205 80-81 1.1627287806568631 2205 82-83 1.1194054292411693 2205 84-85 1.1708347326665667 2205 86-87 1.1895200041149714 2205 88-89 1.2120018008679523 2205 90-91 1.228045466325792 2205 92-93 1.2662762669479797 2205 94-95 1.2728699166532742 2205 96-97 1.271023601867423 2205 98-99 1.2968115596178151 2205 100 1.418542923225779 2206 1 0.6525284029739638 2206 2 0.6401425202548268 2206 3 0.63456985056024 2206 4 0.3263639827280329 2206 5 0.48414770454129297 2206 6 0.49820109386921274 2206 7 0.5317774011441188 2206 8 0.5328848324516713 2206 9 0.7177762438552833 2206 10-11 0.7139590043089825 2206 12-13 0.7339169921256641 2206 14-15 0.9334431976386028 2206 16-17 0.9122495608689576 2206 18-19 0.8958809104272873 2206 20-21 0.8951871676625842 2206 22-23 0.897060244892657 2206 24-25 0.9441296245083777 2206 26-27 0.9438789137392192 2206 28-29 0.9398555367484889 2206 30-31 1.0169951318294324 2206 32-33 1.0450293349035888 2206 34-35 1.1047334006166807 2206 36-37 1.0921652928978745 2206 38-39 1.10507397464999 2206 40-41 1.0906920789519674 2206 42-43 1.1378925450762054 2206 44-45 1.2156513315382114 2206 46-47 1.245294767141658 2206 48-49 1.2363047614070979 2206 50-51 1.2049887285763035 2206 52-53 1.2066340797064186 2206 54-55 1.1683476635105734 2206 56-57 1.225708206965784 2206 58-59 1.2298787445618444 2206 60-61 1.1699081164936658 2206 62-63 1.1795241771277816 2206 64-65 1.2543586735895147 2206 66-67 1.3187301956199278 2206 68-69 1.2916205747698015 2206 70-71 1.254541793245501 2206 72-73 1.2470777084868558 2206 74-75 1.2444420268369782 2206 76-77 1.2064287047895554 2206 78-79 1.0988286901960649 2206 80-81 1.0754728181295192 2206 82-83 1.029818220721367 2206 84-85 1.029482466408762 2206 86-87 1.061707235290239 2206 88-89 1.119973507025648 2206 90-91 1.141514708805392 2206 92-93 1.1530146961462862 2206 94-95 1.1776597725193305 2206 96-97 1.1180998755689338 2206 98-99 1.1868987664656174 2206 100 1.4956601857759466 2207 1 0.658489237825826 2207 2 0.604141212483789 2207 3 0.5956920704999362 2207 4 0.38264479371685667 2207 5 0.4526571723339856 2207 6 0.45667170703549687 2207 7 0.5262252242160272 2207 8 0.5536320682581959 2207 9 0.720416869063115 2207 10-11 0.7216185231464465 2207 12-13 0.7184310243906964 2207 14-15 0.8852131058007018 2207 16-17 0.8806233396162355 2207 18-19 0.8805372514665208 2207 20-21 0.8586362684550508 2207 22-23 0.861705587124213 2207 24-25 0.8740219653446459 2207 26-27 0.9073179258597506 2207 28-29 0.9198608563420123 2207 30-31 0.9748184176255208 2207 32-33 1.0513117448904623 2207 34-35 1.0611872338686439 2207 36-37 1.0406716747680065 2207 38-39 1.0607935106375876 2207 40-41 1.1331800230403175 2207 42-43 1.174582191746488 2207 44-45 1.2109647585568268 2207 46-47 1.1703116212790476 2207 48-49 1.197454137629542 2207 50-51 1.2296534583000636 2207 52-53 1.1412864879083315 2207 54-55 1.1541635244546384 2207 56-57 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0.9880299601673102 2310 82-83 1.0329625547721122 2310 84-85 1.0695550476508195 2310 86-87 1.1218450746859894 2310 88-89 1.1129776626500032 2310 90-91 1.2100902407506204 2310 92-93 1.2425937018724262 2310 94-95 1.2932662415090697 2310 96-97 1.3461240018688976 2310 98-99 1.2661028696380185 2310 100 1.2940713912066002 2311 1 0.32448183517855966 2311 2 0.45517200488205845 2311 3 0.4925439262122637 2311 4 0.3209983436913717 2311 5 -0.05597615774647835 2311 6 0.1505259065267026 2311 7 0.3647352845995826 2311 8 0.17516265767935835 2311 9 0.29395686139520194 2311 10-11 0.47407491378338307 2311 12-13 0.5250399359872446 2311 14-15 0.6606501158748301 2311 16-17 0.815682471869188 2311 18-19 0.8656685611920736 2311 20-21 0.8915210279103007 2311 22-23 0.848449973228476 2311 24-25 0.7952727525040828 2311 26-27 0.8023035502865312 2311 28-29 0.7739457316921161 2311 30-31 0.819191253597964 2311 32-33 0.9492060348537663 2311 34-35 0.9512377147949849 2311 36-37 1.012224122875402 2311 38-39 1.02932930394676 2311 40-41 0.9793900259497761 2311 42-43 1.1090357412074 2311 44-45 1.0644658393054556 2311 46-47 1.088809266371932 2311 48-49 1.074474107334538 2311 50-51 1.1323780244522226 2311 52-53 1.1562849304580496 2311 54-55 1.1223509937323897 2311 56-57 1.1538593677338582 2311 58-59 1.2111793376611502 2311 60-61 1.2696694268832474 2311 62-63 1.267145498362929 2311 64-65 1.2536226500803735 2311 66-67 1.2504190407195281 2311 68-69 1.227823676016321 2311 70-71 1.2340136951752996 2311 72-73 1.2045776914529824 2311 74-75 1.1043797970993623 2311 76-77 1.0753264911479512 2311 78-79 1.0270043647072598 2311 80-81 1.0676702835697967 2311 82-83 1.0073831357985057 2311 84-85 1.076570210679467 2311 86-87 1.1283783842027368 2311 88-89 1.112307398735922 2311 90-91 1.1330322923685436 2311 92-93 1.1839263959702144 2311 94-95 1.2497360990870767 2311 96-97 1.280499571123574 2311 98-99 1.2932088835023379 2311 100 1.4499780514199934 2312 1 0.18856561960086538 2312 2 0.17283723541805784 2312 3 0.3496053898835427 2312 4 0.2596206177698335 2312 5 0.3534969729113939 2312 6 0.3931967126317133 2312 7 0.44810506393250193 2312 8 0.2688890239926991 2312 9 0.4178278190831648 2312 10-11 0.28442682566571875 2312 12-13 0.42968834582417514 2312 14-15 0.8051918188033156 2312 16-17 0.8734724177522324 2312 18-19 0.8601485395944906 2312 20-21 0.8178609127352914 2312 22-23 0.5104111063056322 2312 24-25 0.47035455814457805 2312 26-27 0.7590137690365282 2312 28-29 0.8956838351479135 2312 30-31 0.8503826896023554 2312 32-33 0.6684111466471805 2312 34-35 0.8537498645369084 2312 36-37 0.9493914711475213 2312 38-39 0.9642778575307176 2312 40-41 0.9865429799183332 2312 42-43 0.8223742615800163 2312 44-45 0.8270861906150699 2312 46-47 0.9173107322896072 2312 48-49 1.0005879212741675 2312 50-51 1.0196094396999769 2312 52-53 1.0716091472847893 2312 54-55 1.026296075737477 2312 56-57 1.0387231202331861 2312 58-59 1.1262897006041115 2312 60-61 1.117564459072078 2312 62-63 1.1950091858323262 2312 64-65 1.2260847493199662 2312 66-67 1.2080270743218122 2312 68-69 1.1848771077318858 2312 70-71 1.016909280388056 2312 72-73 1.1579496598491232 2312 74-75 1.1903165140112506 2312 76-77 1.0617488939043191 2312 78-79 0.9749892626703129 2312 80-81 0.9577864303539556 2312 82-83 0.8834999954126204 2312 84-85 0.7425072500457013 2312 86-87 0.9475950759298577 2312 88-89 0.6071951789851404 2312 90-91 1.0305019273859877 2312 92-93 1.1183755471483323 2312 94-95 1.1999867142373652 2312 96-97 1.199116272328304 2312 98-99 1.2447229610606456 2312 100 1.4401625996915826 2313 1 -0.36542574695758034 2313 2 -0.04018513427529058 2313 3 0.24075726425574828 2313 4 0.09047541098367162 2313 5 0.23696257214211158 2313 6 0.37006547802602796 2313 7 0.13579426863741162 2313 8 0.13579064106852456 2313 9 0.33944447486220497 2313 10-11 0.4122451618375891 2313 12-13 0.5818566551660851 2313 14-15 0.6419557217834253 2313 16-17 0.779550749793259 2313 18-19 0.624037755546496 2313 20-21 0.8265675509360975 2313 22-23 0.8816889002251642 2313 24-25 0.7322773338753734 2313 26-27 0.9194642580705761 2313 28-29 0.7899606023339274 2313 30-31 0.792825831018952 2313 32-33 0.923130439554825 2313 34-35 0.8953010970457598 2313 36-37 0.7549476724464412 2313 38-39 0.5088407668829547 2313 40-41 0.677507775862658 2313 42-43 0.7218319349194928 2313 44-45 0.9176773568816827 2313 46-47 0.9282327915391946 2313 48-49 0.997255236366712 2313 50-51 0.9158958493029843 2313 52-53 0.8410081804100287 2313 54-55 0.9388168255936833 2313 56-57 1.0092225465757352 2313 58-59 0.9223588845495314 2313 60-61 0.9474762919818716 2313 62-63 1.018618350269513 2313 64-65 1.1472397517725668 2313 66-67 1.1143505253045376 2313 68-69 1.1045702567095717 2313 70-71 1.0292671171310346 2313 72-73 1.129568820902847 2313 74-75 0.698159669424939 2313 76-77 0.8671332059167121 2313 78-79 0.7281368670510275 2313 80-81 0.78910312209333 2313 82-83 0.8538046676522839 2313 84-85 0.921560165115519 2313 86-87 0.9994249416653354 2313 88-89 0.9234440699189435 2313 90-91 1.022640028387027 2313 92-93 0.7945537715120565 2313 94-95 0.770879857722587 2313 96-97 1.0921870465879486 2313 98-99 1.1641339424552477 2313 100 1.2646308804857114 2314 1 -0.38129851039684937 2314 2 -0.19364750537922149 2314 3 0.13407133914631686 2314 4 -0.2083799226957126 2314 5 0.15641733867349927 2314 6 0.18951415872487587 2314 7 0.08878180659382195 2314 8 0.17773993000510302 2314 9 0.2715361386491182 2314 10-11 0.4565688146123321 2314 12-13 0.4991935750096772 2314 14-15 0.5339259195398114 2314 16-17 0.7706210229153854 2314 18-19 0.8133223643193759 2314 20-21 0.46711182338680146 2314 22-23 0.7187614516876479 2314 24-25 0.8930809051764257 2314 26-27 0.8632223891183131 2314 28-29 0.8110853563264655 2314 30-31 0.8063448468960672 2314 32-33 0.8656277252320521 2314 34-35 0.9253781153066214 2314 36-37 0.8399129111015995 2314 38-39 0.8501861348388005 2314 40-41 0.9094658876439681 2314 42-43 0.7320933488385251 2314 44-45 0.7178311381762086 2314 46-47 0.8824727118272335 2314 48-49 0.769772975693229 2314 50-51 0.8780004942029507 2314 52-53 0.9180832085898203 2314 54-55 0.9874081718899461 2314 56-57 0.8890023472414512 2314 58-59 0.8894576944675094 2314 60-61 1.0284592354802342 2314 62-63 0.7562122838969003 2314 64-65 0.8993033166118636 2314 66-67 0.9532876081618369 2314 68-69 1.0007099131599446 2314 70-71 1.1061188123026042 2314 72-73 1.090418876723426 2314 74-75 1.0849956727310968 2314 76-77 0.8959462736964348 2314 78-79 0.8608770935425838 2314 80-81 0.8709035205149647 2314 82-83 0.7982270896652892 2314 84-85 0.7163274455974395 2314 86-87 0.8669350082819385 2314 88-89 0.9149888076294701 2314 90-91 1.002026981882377 2314 92-93 1.0364341122504932 2314 94-95 1.0595957014481776 2314 96-97 1.1570060741529922 2314 98-99 1.2188134800664976 2314 100 1.1867966481522139 2315 1 -0.1206207362792533 2315 2 0.09219587437424082 2315 3 0.13511175895261118 2315 4 -0.04560877095579485 2315 5 0.2326666849897805 2315 6 0.3205672239042343 2315 7 0.38340390356189147 2315 8 0.3054305783685791 2315 9 0.5435864082808379 2315 10-11 0.5406727977965602 2315 12-13 0.607330162732957 2315 14-15 0.7325599860909833 2315 16-17 0.7377241376546664 2315 18-19 0.7742823749801389 2315 20-21 0.7470492337896815 2315 22-23 0.8690565096244498 2315 24-25 0.809012241302959 2315 26-27 0.8858954052432253 2315 28-29 0.9174351232996543 2315 30-31 0.8804182333737884 2315 32-33 0.9572282033260393 2315 34-35 0.9274158897817841 2315 36-37 0.9907970204944263 2315 38-39 0.9566170924519781 2315 40-41 0.9264787734095208 2315 42-43 1.0078132050048367 2315 44-45 0.8649908688721126 2315 46-47 0.919691376698303 2315 48-49 1.0285293055795535 2315 50-51 0.9340002267622651 2315 52-53 0.9516286835732757 2315 54-55 0.8972570066222474 2315 56-57 0.9944024601987422 2315 58-59 0.9872540689874896 2315 60-61 1.0882878900531878 2315 62-63 1.0496915097142008 2315 64-65 1.1123765532877812 2315 66-67 1.1095225677916076 2315 68-69 1.0210851097604134 2315 70-71 0.7115626216752773 2315 72-73 0.9495649769761769 2315 74-75 1.118298036581379 2315 76-77 1.0184310537921384 2315 78-79 0.9239253071895277 2315 80-81 0.9397930781591342 2315 82-83 0.9373193829179627 2315 84-85 0.8199954562410632 2315 86-87 0.8069319689319876 2315 88-89 0.8833411062576744 2315 90-91 0.9975438070758429 2315 92-93 1.033097633125454 2315 94-95 1.1125929930814067 2315 96-97 1.1781794410979565 2315 98-99 1.0835346586449432 2315 100 1.02304873891487 2316 1 0.49311673920938404 2316 2 0.47343799910616724 2316 3 0.46750311298171 2316 4 0.30998251269220134 2316 5 0.41857907646987513 2316 6 0.398798735765709 2316 7 0.44788965050966567 2316 8 0.4739744604047402 2316 9 0.4774343091114446 2316 10-11 0.4162863741795704 2316 12-13 0.6025932842768711 2316 14-15 0.7686264691116875 2316 16-17 0.7582747375730676 2316 18-19 0.7986112898128752 2316 20-21 0.8417891105178867 2316 22-23 0.8449744996989139 2316 24-25 0.9188623472714781 2316 26-27 0.9438995841119251 2316 28-29 0.8929005127411855 2316 30-31 0.7248932336997242 2316 32-33 0.7456446198284183 2316 34-35 0.6293270556984041 2316 36-37 0.6369856017539419 2316 38-39 0.5209327409721709 2316 40-41 0.6654565317830503 2316 42-43 0.8292243795951819 2316 44-45 0.7831718551075397 2316 46-47 0.8173608711076596 2316 48-49 0.5863838222548949 2316 50-51 0.6968885856978204 2316 52-53 0.7929623985505856 2316 54-55 0.8573238551965474 2316 56-57 0.9198855930684218 2316 58-59 0.8109368980217226 2316 60-61 0.9147318330137537 2316 62-63 0.9469680510758636 2316 64-65 0.9240897784728546 2316 66-67 0.9056823147457749 2316 68-69 0.8228900566436224 2316 70-71 0.696214565310207 2316 72-73 0.8049198387149943 2316 74-75 0.880644494107429 2316 76-77 0.8046956946569246 2316 78-79 0.7405776631048298 2316 80-81 0.724943953237613 2316 82-83 0.6755654370097659 2316 84-85 0.7487194562061568 2316 86-87 0.686933297287208 2316 88-89 0.637342572099552 2316 90-91 0.7119769477806095 2316 92-93 0.7340736482774304 2316 94-95 0.68867455204499 2316 96-97 0.7683818901920709 2316 98-99 0.8534774018117943 2316 100 0.5636180579210013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 5.0 11 11.0 12 49.0 13 241.0 14 924.0 15 2648.0 16 6588.0 17 13755.0 18 22413.0 19 31540.0 20 42371.0 21 55740.0 22 66779.0 23 70979.0 24 73832.0 25 80688.0 26 93963.0 27 114015.0 28 139158.0 29 168996.0 30 206428.0 31 255292.0 32 321187.0 33 415706.0 34 579590.0 35 786000.0 36 1179394.0 37 1846541.0 38 1740110.0 39 274535.0 40 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.270803380617416 19.370257094330974 13.24065449055155 24.118285034500055 2 15.540464552260092 20.941861929854504 40.79592126123957 22.721752256645832 3 17.608112585595606 27.61387775192974 31.18734951134627 23.590660151128386 4 11.887328975159152 17.526143281331855 38.775590048290944 31.81093769521805 5 12.146937942948433 37.973975853565484 34.440109919728044 15.438976283758036 6 28.779527701143685 37.60219867957682 19.940394799755374 13.67787881952412 7 26.46361255020665 33.27324058443448 23.20634495605099 17.056801909307875 8 26.180192304781773 35.29323626897523 20.99611269120751 17.53045873503549 9 27.625518199743127 15.740558775563047 20.48605063571734 36.147872388976495 10-11 24.766380519075234 25.9773255523914 29.361809433981705 19.89448449455166 12-13 25.197897176418387 24.14899426316915 28.784619963552004 21.86848859686046 14-15 22.80358986466758 25.645560238784114 26.91073629759814 24.640113598950162 16-17 21.97837955706318 27.749279513574088 27.563587885897327 22.708753043465403 18-19 21.530932058011633 27.247620035534005 29.39207655283142 21.829371353622946 20-21 22.78335272713302 26.81124603659229 28.722890640507643 21.68251059576705 22-23 22.223131599285804 26.74894407643455 28.770291373142744 22.257632951136898 24-25 21.71967268223895 26.486025836739863 29.256951427685795 22.537350053335388 26-27 21.894585102665555 26.66027825896836 28.921581325328383 22.5235553130377 28-29 21.684942869149282 27.6123257505997 28.888763929372185 21.81396745087883 30-31 22.965049759562728 26.73950947641669 28.85556352851045 21.439877235510124 32-33 22.637508170975345 26.77179464310041 28.65773842306405 21.93295876286019 34-35 22.60871914418561 27.073396725008013 28.913744213579566 21.404139917226807 36-37 22.298794822935484 26.982864912155563 28.80162689441636 21.916713370492598 38-39 22.185222675954115 27.21426797315678 28.771769417357874 21.828739933531235 40-41 22.449412250798375 26.515449579722606 29.108375330803387 21.926762838675636 42-43 22.11300432058154 26.875854328026804 28.487803446895242 22.523337904496415 44-45 21.436481308731132 27.169864505940122 29.008334889638913 22.385319295689833 46-47 21.462025771516846 26.549721527078958 28.66894513881147 23.31930756259273 48-49 21.71976504521975 26.78218342246419 28.79238663802092 22.705664894295147 50-51 21.583695637176906 27.067070508759183 28.67281379580227 22.676420058261638 52-53 21.506218959274825 27.255389028230375 28.254333686511167 22.98405832598363 54-55 21.279839249021503 26.88490372452696 28.767222653797646 23.06803437265389 56-57 21.702865517426453 26.893714703777473 28.256269538187706 23.14715024060837 58-59 21.222389823444715 27.07667898556027 28.672598900002733 23.02833229099228 60-61 21.953783084067403 27.291259126451102 28.070988763750616 22.683969025730878 62-63 21.78584983421504 27.21806078161091 27.99187723530288 23.004212148871172 64-65 21.327571901503642 26.77893804564707 28.116935066226524 23.776554986622767 66-67 21.24022987361098 26.779724761286953 27.94368689379035 24.03635847131172 68-69 20.98576073297478 26.978467421453512 28.07968034859541 23.956091496976303 70-71 21.207713986219776 26.656091371666975 28.263683360339392 23.87251128177386 72-73 21.162577807127423 27.00389639808831 27.792644769736324 24.04088102504794 74-75 21.421074736286744 27.031372157872944 27.483100999921916 24.064452105918395 76-77 21.11831350168939 26.796330270528486 27.621013243325642 24.464342984456486 78-79 20.963529992843906 26.796283673878573 27.96787604608125 24.27231028719627 80-81 21.121627614267286 26.90943571212607 27.636149015578233 24.33278765802842 82-83 21.48969146058186 26.820712593078934 27.688795219769514 24.000800726569693 84-85 21.477674317460277 27.072999256534676 27.20938082488427 24.239945601120777 86-87 21.060791981759785 27.040426070928348 27.502680414772712 24.39610153253916 88-89 21.068860102256835 26.639424038521685 27.661340027908558 24.63037583131292 90-91 21.6671897682495 26.359277036733374 27.618884297111606 24.354648897905516 92-93 21.447237674284768 26.578686855524392 27.52885041662052 24.445225053570322 94-95 21.004178883018522 26.335002464809804 27.70887487544968 24.951943776721997 96-97 20.81665157022407 26.353458171987675 27.855724966877087 24.974165290911166 98-99 20.86999151393941 26.365657509581386 27.645774655062745 25.11857632141646 100 20.723668199601157 26.35599727336856 27.81986160505624 25.100472921974042 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1676.0 1 1221.0 2 2063.0 3 3186.5 4 2252.0 5 1599.5 6 2183.5 7 3944.0 8 6176.0 9 7121.0 10 6440.5 11 5828.0 12 6203.5 13 6893.0 14 7683.5 15 8740.5 16 9772.0 17 10594.0 18 11651.5 19 12858.5 20 14325.0 21 16395.0 22 19012.0 23 22910.5 24 28010.0 25 34442.5 26 43303.0 27 54419.0 28 66872.0 29 81614.5 30 98424.5 31 114542.0 32 134270.0 33 153243.5 34 169277.5 35 186513.5 36 206657.5 37 230299.0 38 243433.0 39 245372.0 40 248116.0 41 252726.5 42 259464.0 43 268786.5 44 281801.5 45 301728.0 46 320142.0 47 335333.5 48 354410.0 49 369030.5 50 376810.5 51 378369.0 52 363996.5 53 339563.0 54 314930.0 55 283244.0 56 249470.0 57 216256.5 58 183110.5 59 155158.5 60 122089.0 61 89533.5 62 67314.0 63 50422.5 64 36107.0 65 24999.5 66 17641.0 67 13094.0 68 9782.5 69 7207.0 70 5331.0 71 3841.5 72 2897.0 73 2140.5 74 1483.0 75 960.0 76 623.0 77 367.0 78 231.5 79 153.0 80 88.5 81 62.5 82 39.5 83 22.0 84 16.0 85 11.0 86 10.0 87 7.0 88 4.0 89 3.0 90 2.0 91 0.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05192374567190549 2 1.1642095442131277E-5 3 1.1642095442131277E-5 4 0.015903102373951324 5 0.003981596641208896 6 0.0 7 2.2119981340049425E-4 8 3.37620767821807E-4 9 0.001944229938835923 10-11 8.20767728670255E-4 12-13 2.3866295656369118E-4 14-15 3.4926286326393826E-5 16-17 1.1059990670024713E-4 18-19 0.0 20-21 4.656838176852511E-5 22-23 5.821047721065639E-6 24-25 7.567362037385329E-5 26-27 2.910523860532819E-5 28-29 8.20767728670255E-4 30-31 7.509151560174673E-4 32-33 0.013889019862462612 34-35 0.022771938684808778 36-37 0.030345121769915172 38-39 0.020094256733118585 40-41 1.5716828846877224E-4 42-43 0.001932587843393792 44-45 0.009348602640031414 46-47 0.013522293856035478 48-49 0.00537864809426465 50-51 0.009878317982648389 52-53 0.015198755599702381 54-55 0.009674581312411092 56-57 0.002910523860532819 58-59 0.007910803852928201 60-61 0.0015309355506402627 62-63 9.197255399283709E-4 64-65 0.018714668423226027 66-67 0.04475803592727369 68-69 0.05433365942842667 70-71 0.0477384123604593 72-73 0.03939685097617224 74-75 0.027923565917951865 76-77 0.027952671156557196 78-79 0.012072852973490134 80-81 0.0018860194616252667 82-83 0.0269863772348603 84-85 0.047127202349747405 86-87 0.04766273874008545 88-89 0.05886243455541574 90-91 0.03392506611837054 92-93 0.02570574673622586 94-95 0.007229741269563522 96-97 0.0034809865371972517 98-99 0.02219565496042328 100 0.019011541857000373 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8589519.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.31333282662875 #Duplication Level Percentage of deduplicated Percentage of total 1 78.77715659040655 50.664214909342675 2 13.54777856468007 17.426055837834724 3 3.8023051505485843 7.3361674996690756 4 1.5083210907276527 3.8802062526956496 5 0.6789761946033572 2.1833610992441783 6 0.40693912766353035 1.570296693456156 7 0.26437908989707626 1.1902170280666309 8 0.17068225863849082 0.8781715925934395 9 0.13160110630128252 0.7617335174916241 >10 0.6566581460441165 7.401837561034753 >50 0.035061382292824045 1.5264023320112874 >100 0.017338592350516203 2.072638469133138 >500 0.0017298897765749195 0.7427551504920961 >1k 9.633450440217705E-4 1.2370627860153258 >5k 4.3788410144156994E-5 0.20883372375301734 >10k+ 6.56826152162355E-5 0.9200455471662162 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27491 0.3200528457996309 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21957 0.25562548962287646 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15892 0.18501618076635024 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 8977 0.10451109078401247 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.1642095442131276E-5 0.0 4 0.0 0.0 0.0 1.1642095442131276E-5 0.0 5 0.0 0.0 0.0 1.1642095442131276E-5 0.0 6 0.0 0.0 0.0 2.328419088426255E-5 0.0 7 0.0 0.0 0.0 3.492628632639383E-5 0.0 8 0.0 0.0 0.0 3.492628632639383E-5 0.0 9 0.0 0.0 0.0 8.149466809491894E-5 0.0 10-11 0.0 5.821047721065638E-6 0.0 1.222420021423784E-4 0.0 12-13 0.0 1.1642095442131276E-5 0.0 1.9209457479516608E-4 0.0 14-15 0.0 2.910523860532819E-5 0.0 2.444840042847568E-4 3.4926286326393826E-5 16-17 0.0 4.65683817685251E-5 0.0 2.9687343377434757E-4 6.985257265278766E-5 18-19 0.0 5.2389429489590745E-5 0.0 4.074733404745947E-4 8.731571581598458E-5 20-21 0.0 5.821047721065638E-5 0.0 7.509151560174674E-4 1.1059990670024713E-4 22-23 5.821047721065638E-6 5.821047721065638E-5 0.0 0.0018569142230199388 1.3388409758450967E-4 24-25 1.1642095442131276E-5 6.403152493172202E-5 0.0 0.004517133031546935 1.513472407477066E-4 26-27 1.1642095442131276E-5 7.56736203738533E-5 0.0 0.008586045388571816 1.5716828846877224E-4 28-29 1.1642095442131276E-5 8.149466809491894E-5 0.0 0.020292172355634814 1.8045247935303478E-4 30-31 1.1642095442131276E-5 8.149466809491894E-5 0.0 0.06241909471298684 1.862735270741004E-4 32-33 1.1642095442131276E-5 8.149466809491894E-5 0.0 0.1561030367358172 1.979156225162317E-4 34-35 1.1642095442131276E-5 9.31367635370502E-5 0.0 0.2799283638583255 1.979156225162317E-4 36-37 1.1642095442131276E-5 1.1059990670024713E-4 0.0 0.4678434263897664 1.979156225162317E-4 38-39 1.1642095442131276E-5 1.2806304986344403E-4 0.0 0.7998352410653029 2.1537876567942862E-4 40-41 1.1642095442131276E-5 1.2806304986344403E-4 0.0 1.218420961639412 2.2119981340049425E-4 42-43 1.1642095442131276E-5 1.3970514530557533E-4 0.0 1.6032795317176665 2.2119981340049425E-4 44-45 1.1642095442131276E-5 1.3970514530557533E-4 0.0 2.0062590233515984 2.2119981340049425E-4 46-47 1.1642095442131276E-5 1.3970514530557533E-4 0.0 2.4615639129501896 2.3284190884262552E-4 48-49 1.1642095442131276E-5 1.4552619302664097E-4 0.0 2.9535937926209836 2.3284190884262552E-4 50-51 1.1642095442131276E-5 1.5716828846877224E-4 0.0 3.4958476720291323 2.5612609972688806E-4 52-53 1.1642095442131276E-5 1.6298933618983787E-4 0.0 4.052840444267019 2.7358924289008497E-4 54-55 1.1642095442131276E-5 1.7463143163196914E-4 0.0 4.631889166320024 2.9687343377434757E-4 56-57 1.1642095442131276E-5 1.862735270741004E-4 0.0 5.235857793666909 3.317997201007414E-4 58-59 1.1642095442131276E-5 1.862735270741004E-4 0.0 5.919208048785968 3.492628632639383E-4 60-61 1.1642095442131276E-5 2.0373667023729735E-4 0.0 6.591707870952961 3.667260064271352E-4 62-63 1.1642095442131276E-5 2.0955771795836298E-4 0.0 7.335911358948039 3.958312450324634E-4 64-65 1.1642095442131276E-5 2.0955771795836298E-4 0.0 7.9945221612525685 3.958312450324634E-4 66-67 1.1642095442131276E-5 2.0955771795836298E-4 0.0 8.696604547938016 3.958312450324634E-4 68-69 1.1642095442131276E-5 2.0955771795836298E-4 0.0 9.464942099784633 4.074733404745947E-4 70-71 1.1642095442131276E-5 2.0955771795836298E-4 0.0 10.269154768736177 4.074733404745947E-4 72-73 1.1642095442131276E-5 2.0955771795836298E-4 0.0 11.004428769527141 5.006101040116449E-4 74-75 1.1642095442131276E-5 2.0955771795836298E-4 0.0 11.715789906279968 5.006101040116449E-4 76-77 1.1642095442131276E-5 2.0955771795836298E-4 0.0 12.473812561564856 5.006101040116449E-4 78-79 1.1642095442131276E-5 2.0955771795836298E-4 0.0 13.296524520173946 5.006101040116449E-4 80-81 1.1642095442131276E-5 2.0955771795836298E-4 0.0 14.080736069155911 5.064311517327105E-4 82-83 1.1642095442131276E-5 2.2119981340049425E-4 0.0 14.872491695984372 5.180732471748418E-4 84-85 1.1642095442131276E-5 2.2119981340049425E-4 0.0 15.647180010894672 5.238942948959074E-4 86-87 1.1642095442131276E-5 2.2119981340049425E-4 0.0 16.441223309477515 5.238942948959074E-4 88 1.1642095442131276E-5 2.2119981340049425E-4 0.0 17.047206019335892 5.238942948959074E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 11425 0.0 59.519016 2 ACCTGGG 13035 0.0 50.61883 3 GGTATCA 16365 0.0 42.778137 1 CCTGGGG 10795 0.0 40.662155 4 TAACGCA 1230 0.0 40.119026 4 ATAACGC 1215 0.0 39.839787 3 GTACCTG 17455 0.0 39.702774 1 GTATCAA 27180 0.0 36.56779 1 CTGGGGG 7470 0.0 35.10117 5 TGGGGGG 17640 0.0 30.578836 6 ATCAACG 33760 0.0 28.96848 3 GTACATG 29270 0.0 28.864801 1 CAACGCA 33985 0.0 28.842642 5 TATCAAC 34765 0.0 28.30596 2 TCAACGC 34780 0.0 28.268208 4 TACATGG 29575 0.0 28.029621 2 AACGCAG 36405 0.0 27.46555 6 ACATGGG 29255 0.0 26.955532 3 CATGGGG 21500 0.0 26.755259 4 TATAACG 1960 0.0 26.614008 2 >>END_MODULE