FastQCFastQC Report
Mon 6 Jun 2016
ERR1211432_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211432_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9265649
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308120.33254011672576844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234780.2533875392862389No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT170130.183613689661674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157240.16970208994534544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG104100.051.1358382
ACCTGGG123250.042.6801873
GTACCTG155200.034.971061
CCTGGGG118800.032.5264134
TAACGCA11600.030.3937364
GTACATG331750.029.6387311
TACATGG340550.028.1943952
ATAACGC12600.027.6085993
ACATGGG343450.027.0462193
TGGGGGG208850.026.6276876
CTGGGGG84950.026.008645
CATGGGG257350.025.9750424
GTATCAA463700.025.5635781
ATGGGGG193150.025.3604055
GGTATCA335850.022.8940621
CAACGCA528550.021.9148145
ATCAACG530800.021.8927693
TATAACG18300.021.6043742
TATCAAC541050.021.5477582
TCAACGC541300.021.4593014
Produced by FastQC (version 0.11.5)