##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211432_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9265649 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21051477343897 31.0 31.0 34.0 28.0 34.0 2 31.25722806896743 31.0 31.0 34.0 28.0 34.0 3 31.32289006414985 31.0 31.0 34.0 28.0 34.0 4 34.93969348504352 37.0 35.0 37.0 32.0 37.0 5 34.99530944891178 37.0 35.0 37.0 32.0 37.0 6 35.05958028412257 37.0 35.0 37.0 32.0 37.0 7 34.98712783098086 37.0 35.0 37.0 32.0 37.0 8 34.98547678635355 37.0 35.0 37.0 32.0 37.0 9 36.53364939682045 39.0 37.0 39.0 32.0 39.0 10-11 36.454519321852146 39.0 37.0 39.0 32.0 39.0 12-13 36.35786262786341 39.0 37.0 39.0 32.0 39.0 14-15 37.51253371458384 40.0 37.0 41.0 32.0 41.0 16-17 37.508064680628415 40.0 37.0 41.0 32.0 41.0 18-19 37.50773032736293 40.0 37.0 41.0 31.5 41.0 20-21 37.490902957796045 40.0 37.0 41.0 31.0 41.0 22-23 37.365952940803176 40.0 37.0 41.0 31.0 41.0 24-25 37.269357656436156 40.0 36.5 41.0 31.0 41.0 26-27 37.129174113977335 40.0 36.0 41.0 30.5 41.0 28-29 36.999431556278466 39.5 36.0 41.0 30.0 41.0 30-31 36.844674183103635 39.0 36.0 41.0 30.0 41.0 32-33 36.64085089992077 39.0 35.0 41.0 30.0 41.0 34-35 36.515756046878096 39.0 35.0 41.0 30.0 41.0 36-37 36.38587291618752 39.0 35.0 41.0 29.0 41.0 38-39 36.236292892165466 39.0 35.0 40.0 29.0 41.0 40-41 36.08133267297305 39.0 35.0 40.0 28.0 41.0 42-43 35.88907733284522 38.0 35.0 40.0 27.0 41.0 44-45 35.75337885128177 38.0 35.0 40.0 27.0 41.0 46-47 35.531965650760135 38.0 34.0 40.0 26.0 41.0 48-49 35.285463975594155 38.0 34.0 40.0 25.5 41.0 50-51 34.61874332817917 37.5 33.5 39.5 24.5 40.5 52-53 34.933815483405425 38.0 33.5 39.5 25.5 40.5 54-55 35.349402022459515 38.0 34.0 40.0 26.0 41.0 56-57 35.26977441083728 38.0 34.0 40.0 26.0 41.0 58-59 35.07004549816209 38.0 34.0 40.0 26.0 41.0 60-61 34.835104642966726 37.5 34.0 40.0 26.0 41.0 62-63 34.56060120559283 37.0 33.0 40.0 25.0 41.0 64-65 34.35593227198656 37.0 33.0 40.0 25.0 41.0 66-67 34.15846170084794 36.0 33.0 39.5 25.0 41.0 68-69 33.83509352663802 36.0 33.0 39.0 24.0 41.0 70-71 33.47761338682266 35.0 32.0 39.0 24.0 40.5 72-73 33.115186696582185 35.0 32.0 38.0 23.0 40.0 74-75 32.77254491293594 35.0 32.0 37.0 23.0 39.5 76-77 32.429637578544146 35.0 31.5 37.0 22.0 39.0 78-79 32.07662355869513 35.0 31.0 36.0 21.5 39.0 80-81 31.730070716039428 35.0 31.0 36.0 20.0 38.0 82-83 31.412526149004783 35.0 31.0 35.0 20.0 37.0 84-85 31.123598789464182 34.0 30.0 35.0 19.5 37.0 86-87 30.847652819570435 34.0 30.0 35.0 19.0 36.0 88-89 30.58885950676526 34.0 30.0 35.0 18.0 36.0 90-91 30.339142406538386 34.0 29.0 35.0 18.0 36.0 92-93 30.107101024439842 34.0 29.0 35.0 17.0 35.0 94-95 29.8777104010739 34.0 29.0 35.0 15.5 35.0 96-97 29.634086398049398 34.0 29.0 35.0 14.0 35.0 98-99 29.37716089828138 34.0 27.5 35.0 10.5 35.0 100 28.158586408787986 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.32753334672763046 1101 2 -0.30294408152575514 1101 3 -0.4037312788235248 1101 4 -0.27783065023375997 1101 5 -0.2624944045769908 1101 6 -0.33852624795639485 1101 7 -0.3417382823781381 1101 8 -0.28441638131810265 1101 9 -0.559079087775828 1101 10-11 -0.43572102502461263 1101 12-13 -0.5833459545749164 1101 14-15 -0.730503716145904 1101 16-17 -0.6379980603989068 1101 18-19 -0.6347088414848514 1101 20-21 -0.7343696087818685 1101 22-23 -0.7019233099009483 1101 24-25 -0.7738275996066548 1101 26-27 -0.8053751439528867 1101 28-29 -0.825190155960442 1101 30-31 -0.837441139477356 1101 32-33 -0.8239273618554961 1101 34-35 -0.8861027710110321 1101 36-37 -0.8882278378767552 1101 38-39 -0.8590185129753891 1101 40-41 -1.0211198728850093 1101 42-43 -0.9667194382208137 1101 44-45 -0.9423128813138248 1101 46-47 -0.98959156955884 1101 48-49 -1.0102996289853294 1101 50-51 -0.8982565614263649 1101 52-53 -0.7731864382388167 1101 54-55 -0.7389641768141075 1101 56-57 -0.7420675548954847 1101 58-59 -0.7048922544872411 1101 60-61 -0.7825596121837819 1101 62-63 -0.8233444087515949 1101 64-65 -0.731067694479627 1101 66-67 -0.6953110686063368 1101 68-69 -0.8928318403354396 1101 70-71 -0.8758100938134916 1101 72-73 -0.9431730076248996 1101 74-75 -0.9483793923314536 1101 76-77 -0.8562161489066575 1101 78-79 -0.8799980012701631 1101 80-81 -1.0724615135288964 1101 82-83 -1.1273342978904424 1101 84-85 -1.135232435566465 1101 86-87 -1.153955502094334 1101 88-89 -1.2520371368044643 1101 90-91 -1.3988321208244336 1101 92-93 -1.4745664184387266 1101 94-95 -1.544936204090849 1101 96-97 -1.6407783452022144 1101 98-99 -1.7159282724503981 1101 100 -1.7598669227676815 1102 1 -0.4734500499422829 1102 2 -0.43311045100898937 1102 3 -0.5325599994994086 1102 4 -0.4482110783824069 1102 5 -0.34212370145364446 1102 6 -0.36829436999470744 1102 7 -0.45209518719852326 1102 8 -0.4169831062586695 1102 9 -0.5470952423874067 1102 10-11 -0.561616069447183 1102 12-13 -0.6261351332230376 1102 14-15 -0.851821715023938 1102 16-17 -0.8426060571895206 1102 18-19 -0.8431478378818511 1102 20-21 -0.9178394103963896 1102 22-23 -0.8711389203359161 1102 24-25 -0.8622643927084468 1102 26-27 -0.937641785316103 1102 28-29 -1.033579373298977 1102 30-31 -0.9470072775397114 1102 32-33 -0.9467033112014391 1102 34-35 -1.0110014922079884 1102 36-37 -1.108010080638195 1102 38-39 -1.1487392095679496 1102 40-41 -1.237724438950842 1102 42-43 -1.198993317698772 1102 44-45 -1.2466674909873916 1102 46-47 -1.3512681674608302 1102 48-49 -1.338496331261588 1102 50-51 -1.1293031815161214 1102 52-53 -1.105018740978167 1102 54-55 -1.0792563665438308 1102 56-57 -1.0847477710515108 1102 58-59 -0.9963050305957566 1102 60-61 -1.0096095702480525 1102 62-63 -1.0258799165311387 1102 64-65 -1.1302576805451636 1102 66-67 -1.1405241565392572 1102 68-69 -1.1440144095391247 1102 70-71 -1.161866528860351 1102 72-73 -1.159678798477504 1102 74-75 -1.2289226945366707 1102 76-77 -1.163987677025311 1102 78-79 -1.2154804732889488 1102 80-81 -1.3359063831331603 1102 82-83 -1.346644252303797 1102 84-85 -1.4601943704490559 1102 86-87 -1.4001201556705531 1102 88-89 -1.5542632015633409 1102 90-91 -1.530441129902698 1102 92-93 -1.5547431011462045 1102 94-95 -1.6575880991487004 1102 96-97 -1.7644842479651146 1102 98-99 -1.874826805396463 1102 100 -1.953469118296475 1103 1 -0.4926892594861947 1103 2 -0.5606708961746705 1103 3 -0.594929298160551 1103 4 -0.45599260016874155 1103 5 -0.45566314651019724 1103 6 -0.5307052012027 1103 7 -0.4949234404659393 1103 8 -0.5609580769169966 1103 9 -0.6061494133243315 1103 10-11 -0.6700124439254225 1103 12-13 -0.7036633158358327 1103 14-15 -0.919251717207267 1103 16-17 -0.9618157495196158 1103 18-19 -0.9449754585647696 1103 20-21 -0.8612386556401788 1103 22-23 -0.8803029557772817 1103 24-25 -0.9350952643739774 1103 26-27 -0.9767643746736496 1103 28-29 -0.9331299362352965 1103 30-31 -0.9605280380443446 1103 32-33 -1.0159032103535992 1103 34-35 -0.9558605308871719 1103 36-37 -1.0228693371271262 1103 38-39 -1.1043686650130127 1103 40-41 -1.0960214635457461 1103 42-43 -1.044413676334777 1103 44-45 -1.140080796112116 1103 46-47 -1.1704535276056731 1103 48-49 -1.2665717037334687 1103 50-51 -1.1809795565957444 1103 52-53 -1.0273652764552565 1103 54-55 -1.0108733227932873 1103 56-57 -1.0395006042097634 1103 58-59 -0.9943526525634994 1103 60-61 -0.9503851924191267 1103 62-63 -0.9720614816921795 1103 64-65 -0.9686393835679326 1103 66-67 -1.0416193788589894 1103 68-69 -0.9900363815588591 1103 70-71 -1.0313518221175002 1103 72-73 -1.0041398717585892 1103 74-75 -1.1319871675718645 1103 76-77 -1.162536591849502 1103 78-79 -1.1690875404250498 1103 80-81 -1.1695644920978943 1103 82-83 -1.2568431924597263 1103 84-85 -1.3091933212448978 1103 86-87 -1.4513577721467925 1103 88-89 -1.453748662514716 1103 90-91 -1.3771664264013843 1103 92-93 -1.379526041064004 1103 94-95 -1.3918241116254784 1103 96-97 -1.5744400989476688 1103 98-99 -1.5906755514772968 1103 100 -1.7135672918419402 1104 1 -0.4524873814326753 1104 2 -0.4674330695764404 1104 3 -0.4202990292209243 1104 4 -0.30214528143028474 1104 5 -0.3797408113478724 1104 6 -0.3789487152318074 1104 7 -0.35535579504859527 1104 8 -0.44581608335749223 1104 9 -0.5234288374556542 1104 10-11 -0.5021638648220801 1104 12-13 -0.6135729416323983 1104 14-15 -0.7405772558656594 1104 16-17 -0.7072306573846063 1104 18-19 -0.8048057143241039 1104 20-21 -0.826408595525713 1104 22-23 -0.7935927209416462 1104 24-25 -0.7624423679030699 1104 26-27 -0.7613369935896941 1104 28-29 -0.9696066260063674 1104 30-31 -0.9930391160064289 1104 32-33 -0.8997291111266392 1104 34-35 -0.9492088796787428 1104 36-37 -1.0185976749488574 1104 38-39 -1.0541899828906267 1104 40-41 -0.970171887683243 1104 42-43 -0.9698848063589907 1104 44-45 -1.0758114473486913 1104 46-47 -1.1162963485713888 1104 48-49 -1.119833893267355 1104 50-51 -0.9788504392149946 1104 52-53 -0.9410304532461495 1104 54-55 -0.9157502415718284 1104 56-57 -0.8687166860813136 1104 58-59 -0.902707780278412 1104 60-61 -0.8817127767678201 1104 62-63 -0.8820166069357924 1104 64-65 -0.8980789879945874 1104 66-67 -0.8688210873536164 1104 68-69 -0.9527338507379284 1104 70-71 -0.84555064948389 1104 72-73 -0.8279567248141859 1104 74-75 -0.9203142116661027 1104 76-77 -1.0720503079282828 1104 78-79 -0.9625757169530154 1104 80-81 -1.0372613880717978 1104 82-83 -1.0561219973921752 1104 84-85 -1.0249776958780643 1104 86-87 -1.0716457133517352 1104 88-89 -1.162218788228568 1104 90-91 -1.1188150605598821 1104 92-93 -1.1948189008497856 1104 94-95 -1.2097145038145136 1104 96-97 -1.3269908001457686 1104 98-99 -1.3225582524200306 1104 100 -1.1968498696313716 1105 1 -0.46853848117869035 1105 2 -0.5093323663654665 1105 3 -0.509775156066901 1105 4 -0.3517935457134129 1105 5 -0.3981122228522338 1105 6 -0.335008622678977 1105 7 -0.41506574408387564 1105 8 -0.36203481730356657 1105 9 -0.4356053643494704 1105 10-11 -0.5010057720697887 1105 12-13 -0.6234986519289407 1105 14-15 -0.7486837817553393 1105 16-17 -0.7513240656256741 1105 18-19 -0.7388635387670917 1105 20-21 -0.7532634065696797 1105 22-23 -0.7043947735129947 1105 24-25 -0.6886076490418063 1105 26-27 -0.7796532558617884 1105 28-29 -0.7183826605599677 1105 30-31 -0.7574290250540585 1105 32-33 -0.850955523537472 1105 34-35 -0.8770351150444995 1105 36-37 -0.8239072862070813 1105 38-39 -0.8554485039987796 1105 40-41 -0.8877164925207595 1105 42-43 -0.8770850917696364 1105 44-45 -0.7861349161709725 1105 46-47 -1.0143733909384878 1105 48-49 -1.011634814546042 1105 50-51 -0.9400757789369436 1105 52-53 -0.8098832690202329 1105 54-55 -0.7018948845874746 1105 56-57 -0.6520772661695347 1105 58-59 -0.7346491518328335 1105 60-61 -0.7460396679131946 1105 62-63 -0.8663623952267585 1105 64-65 -0.7653671036640404 1105 66-67 -0.7568282198779883 1105 68-69 -0.76042910153636 1105 70-71 -0.7913676323081873 1105 72-73 -0.6990519538846698 1105 74-75 -0.7242695686161831 1105 76-77 -0.7065896977156676 1105 78-79 -0.8400100987226509 1105 80-81 -0.8207950857537973 1105 82-83 -0.8919405121395911 1105 84-85 -0.8068170111941058 1105 86-87 -0.8230336733956136 1105 88-89 -0.9805903299635332 1105 90-91 -0.997843053627502 1105 92-93 -0.9970229920783744 1105 94-95 -1.0521391919721985 1105 96-97 -1.1177828935032892 1105 98-99 -1.182039125828691 1105 100 -1.1800298061679442 1106 1 -0.5125511366214219 1106 2 -0.5242467868317959 1106 3 -0.5616300711969302 1106 4 -0.3773355851141602 1106 5 -0.4188657964454663 1106 6 -0.4812686264676813 1106 7 -0.4510748359841372 1106 8 -0.4355793654626865 1106 9 -0.7368482479707907 1106 10-11 -0.7688397213038058 1106 12-13 -0.6493084158539517 1106 14-15 -0.7896992198397825 1106 16-17 -0.823197701398179 1106 18-19 -0.8043555736996097 1106 20-21 -0.7991436402871059 1106 22-23 -0.7163597245832491 1106 24-25 -0.8359951752448112 1106 26-27 -0.8037190072023606 1106 28-29 -0.8674220218141784 1106 30-31 -0.8851641850993843 1106 32-33 -0.9647930017917687 1106 34-35 -0.9054447223960693 1106 36-37 -0.9474252148601963 1106 38-39 -1.0212279880820816 1106 40-41 -1.0806176862894645 1106 42-43 -0.9706010632547404 1106 44-45 -1.067920672477939 1106 46-47 -1.046300643172927 1106 48-49 -1.0895915666500358 1106 50-51 -1.0160435242291328 1106 52-53 -0.9352451033919209 1106 54-55 -0.8362086868989778 1106 56-57 -0.905476097626071 1106 58-59 -0.9003574816884523 1106 60-61 -0.8220575099877081 1106 62-63 -0.8654633023957103 1106 64-65 -0.953188188738082 1106 66-67 -0.908890464812508 1106 68-69 -0.8462552749741121 1106 70-71 -0.8489125231765442 1106 72-73 -0.8183855414550223 1106 74-75 -0.8246906273943679 1106 76-77 -0.8735398979225408 1106 78-79 -0.8771395778133453 1106 80-81 -0.8558190251468076 1106 82-83 -0.9424586345430512 1106 84-85 -0.8796417347543652 1106 86-87 -0.946680063390211 1106 88-89 -0.9966157218559708 1106 90-91 -0.9186747883065287 1106 92-93 -0.9544635598286177 1106 94-95 -0.9446930375806488 1106 96-97 -1.075501325306444 1106 98-99 -1.1843738257019183 1106 100 -1.1180516129415494 1107 1 -0.4947628294444968 1107 2 -0.4828031539860014 1107 3 -0.5138174359394441 1107 4 -0.3810145216093872 1107 5 -0.3739748706620958 1107 6 -0.3178520212645921 1107 7 -0.39125506987670633 1107 8 -0.42773479385739677 1107 9 -0.5306864890421323 1107 10-11 -0.5676781375248652 1107 12-13 -0.5961237749581514 1107 14-15 -0.7523820820526197 1107 16-17 -0.7915321506393624 1107 18-19 -0.7642957377751429 1107 20-21 -0.8271791930467245 1107 22-23 -0.7833207300780032 1107 24-25 -0.7818772644026737 1107 26-27 -0.8313205741583403 1107 28-29 -0.8563888590422906 1107 30-31 -0.8166446896466155 1107 32-33 -0.8709683079599202 1107 34-35 -0.7967357681408771 1107 36-37 -0.8127171993752427 1107 38-39 -0.774064668010702 1107 40-41 -0.7361549585787159 1107 42-43 -0.7241102025776271 1107 44-45 -0.8426811136037742 1107 46-47 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1.5847359701274613 2310 100 1.7828364802638674 2311 1 0.6747270005778567 2311 2 0.7117677379838128 2311 3 0.7431957940256524 2311 4 0.6154732127620548 2311 5 0.6180368040914033 2311 6 0.6212762859303851 2311 7 0.6567493737354866 2311 8 0.6896664936629051 2311 9 0.8487026301655618 2311 10-11 0.7761577912077229 2311 12-13 0.9112042819807726 2311 14-15 1.1319181451583944 2311 16-17 1.1163232718343252 2311 18-19 1.1363651152182541 2311 20-21 1.1443877412322294 2311 22-23 1.1749498851668534 2311 24-25 1.180456348368331 2311 26-27 1.225016300738119 2311 28-29 1.2663930286553722 2311 30-31 1.2542661165324063 2311 32-33 1.3060693839954638 2311 34-35 1.2974756737391147 2311 36-37 1.3107005578489819 2311 38-39 1.3027616099821202 2311 40-41 1.4184100338585566 2311 42-43 1.4059865418548796 2311 44-45 1.4288357431252834 2311 46-47 1.4392756179307966 2311 48-49 1.4673871298636598 2311 50-51 1.4395222206201765 2311 52-53 1.3058838829406554 2311 54-55 1.211774812055225 2311 56-57 1.205696139250577 2311 58-59 1.1825777546970428 2311 60-61 1.1787016291507584 2311 62-63 1.1692377014911273 2311 64-65 1.201921012005414 2311 66-67 1.2389961755749894 2311 68-69 1.203303127020959 2311 70-71 1.1652656106355153 2311 72-73 1.1981521762796064 2311 74-75 1.1877143717164884 2311 76-77 1.1969377845426763 2311 78-79 1.1961811981136954 2311 80-81 1.2316985460482819 2311 82-83 1.2899900914394493 2311 84-85 1.249125121949362 2311 86-87 1.3017479656297866 2311 88-89 1.3158839764698946 2311 90-91 1.342501740810846 2311 92-93 1.393124941835353 2311 94-95 1.4196441631797825 2311 96-97 1.4660522276738845 2311 98-99 1.5784294150192189 2311 100 1.699288584448599 2312 1 0.647954880996295 2312 2 0.6764345469372373 2312 3 0.7193830506046304 2312 4 0.6041611328032701 2312 5 0.5831804496402668 2312 6 0.48869847173347836 2312 7 0.5877053682648565 2312 8 0.6437431942615675 2312 9 0.8310409059202115 2312 10-11 0.8633960862687644 2312 12-13 0.8893748220130533 2312 14-15 1.0710380523713638 2312 16-17 1.1065970588518326 2312 18-19 1.0779502215769128 2312 20-21 1.1203085779781148 2312 22-23 1.134781198464637 2312 24-25 1.1626649367389135 2312 26-27 1.149526164764211 2312 28-29 1.2114172904873897 2312 30-31 1.1375369178139891 2312 32-33 1.2461131714639322 2312 34-35 1.2844235084623037 2312 36-37 1.2402565878322847 2312 38-39 1.2975312083032762 2312 40-41 1.311807752296616 2312 42-43 1.3588717577965141 2312 44-45 1.3894183429786864 2312 46-47 1.3551145615118756 2312 48-49 1.4030619130408795 2312 50-51 1.39031470314103 2312 52-53 1.2219813700137934 2312 54-55 1.1913724619545576 2312 56-57 1.1609239826772324 2312 58-59 1.2052177005477134 2312 60-61 1.1576012980742618 2312 62-63 1.1493291210800294 2312 64-65 1.1504147625756147 2312 66-67 1.138205361840896 2312 68-69 1.1805119720480235 2312 70-71 1.2119356037920355 2312 72-73 1.1892988032001952 2312 74-75 1.1827480581412715 2312 76-77 1.138819569184399 2312 78-79 1.174874984885971 2312 80-81 0.9960083094484027 2312 82-83 1.172846401832956 2312 84-85 1.2172681197263273 2312 86-87 1.2613945569094085 2312 88-89 1.2956890339121117 2312 90-91 1.3136745321466208 2312 92-93 1.3862809168713035 2312 94-95 1.418095965479882 2312 96-97 1.448738463134088 2312 98-99 1.3527446170432071 2312 100 1.408922687883038 2313 1 0.5931213087082483 2313 2 0.5861326181083015 2313 3 0.6865849277104843 2313 4 0.5718977908350809 2313 5 0.5896821055369514 2313 6 0.6144918670502477 2313 7 0.6800126229100059 2313 8 0.6767358464071265 2313 9 0.854715009875342 2313 10-11 0.7195605689427822 2313 12-13 0.836525728135264 2313 14-15 1.014883677630067 2313 16-17 1.035016022545797 2313 18-19 1.0376309935483476 2313 20-21 1.0745849361024398 2313 22-23 1.1320163733577004 2313 24-25 1.196817360833144 2313 26-27 1.2064274408365208 2313 28-29 1.2041393530765987 2313 30-31 1.1716034885374143 2313 32-33 1.2345837552567573 2313 34-35 1.2256335444634843 2313 36-37 1.2714484174821834 2313 38-39 1.315809447613411 2313 40-41 1.3024272367626466 2313 42-43 1.33792055829295 2313 44-45 1.2813829216222317 2313 46-47 1.27423293362488 2313 48-49 1.2735816311924921 2313 50-51 1.2701789743327652 2313 52-53 1.1354225422107476 2313 54-55 1.1376035568743674 2313 56-57 1.1609780824553155 2313 58-59 1.1449379785382092 2313 60-61 1.1374070396707836 2313 62-63 1.1426325376261701 2313 64-65 1.0953281593634117 2313 66-67 1.0880370218095763 2313 68-69 1.1332058818929056 2313 70-71 1.1214036677547483 2313 72-73 1.133761683316017 2313 74-75 1.1819557992278007 2313 76-77 1.154048001770967 2313 78-79 1.144955113904846 2313 80-81 1.2019201911221238 2313 82-83 1.2029217270379178 2313 84-85 1.2383663843951496 2313 86-87 1.2398100101745086 2313 88-89 1.2758572707124287 2313 90-91 1.3554362635182216 2313 92-93 1.4241829380710378 2313 94-95 1.4463596482335603 2313 96-97 1.4648949112006093 2313 98-99 1.5322825550884964 2313 100 1.772060206956649 2314 1 0.5315047487008115 2314 2 0.48526617282974627 2314 3 0.5267588005283343 2314 4 0.436043537109974 2314 5 0.4657344655671025 2314 6 0.4832166631811319 2314 7 0.5508890976145864 2314 8 0.5475605183536487 2314 9 0.6887548916218051 2314 10-11 0.6699318710978233 2314 12-13 0.45386384390314305 2314 14-15 0.8307162144224094 2314 16-17 0.8633734581424903 2314 18-19 0.8923337004141558 2314 20-21 0.8737773208026667 2314 22-23 0.7426585534100596 2314 24-25 0.8062537286749105 2314 26-27 0.9236646614125164 2314 28-29 1.0025395014208627 2314 30-31 0.9440864496733354 2314 32-33 0.9941211654573365 2314 34-35 1.0572088216953048 2314 36-37 1.0760584950317877 2314 38-39 1.1044033976301293 2314 40-41 1.098261897664294 2314 42-43 1.1982265845578226 2314 44-45 1.3338434913103043 2314 46-47 1.305440318068797 2314 48-49 1.3480112673737779 2314 50-51 1.251344109604922 2314 52-53 1.0922952008769258 2314 54-55 1.032717173420636 2314 56-57 1.0559567224023567 2314 58-59 1.0750036297040282 2314 60-61 1.0101913496161004 2314 62-63 0.9964443204480133 2314 64-65 1.0098138367102294 2314 66-67 1.0169635028546011 2314 68-69 1.0183380610941484 2314 70-71 1.0269028052339237 2314 72-73 0.9996148970614982 2314 74-75 1.0725771698767872 2314 76-77 1.0629082110962997 2314 78-79 1.1220525430703354 2314 80-81 1.1630565577129808 2314 82-83 1.0590436749491658 2314 84-85 1.0323641322506205 2314 86-87 1.1843350939186266 2314 88-89 1.2877406443572355 2314 90-91 1.3950392145094312 2314 92-93 1.4086375116879815 2314 94-95 1.458624588926515 2314 96-97 1.4827361906894936 2314 98-99 1.5801167607537856 2314 100 1.507667465523788 2315 1 0.4019193706912887 2315 2 0.42242039296863965 2315 3 0.47321735380154095 2315 4 0.42168613414878564 2315 5 0.391364640026147 2315 6 -0.3224486280942145 2315 7 0.26435992850014145 2315 8 0.3339276166202083 2315 9 0.5366192050028147 2315 10-11 0.6203903215425157 2315 12-13 0.6519115934340007 2315 14-15 0.7378679374116786 2315 16-17 0.6500213078311887 2315 18-19 0.7979908197813401 2315 20-21 0.84285702321408 2315 22-23 0.7293244300722961 2315 24-25 0.8366476538287131 2315 26-27 0.8919814138482351 2315 28-29 0.9721656782016197 2315 30-31 1.0193548372862864 2315 32-33 1.0186581273097985 2315 34-35 0.9673402047092097 2315 36-37 1.0209388865221598 2315 38-39 1.0698870246683967 2315 40-41 1.1031317872131936 2315 42-43 1.1489106980085424 2315 44-45 1.173184360550259 2315 46-47 1.1785189261481577 2315 48-49 1.1658224889389146 2315 50-51 1.0819368072293685 2315 52-53 1.0010952984920465 2315 54-55 0.7960502212969089 2315 56-57 0.824935015591393 2315 58-59 0.805770971880456 2315 60-61 0.7347478097054037 2315 62-63 0.9379339697508158 2315 64-65 0.9539555220855291 2315 66-67 0.9886594632533061 2315 68-69 0.9669991155309674 2315 70-71 0.9835524236369935 2315 72-73 1.0424044354838244 2315 74-75 1.1053651522782388 2315 76-77 1.0551745714843221 2315 78-79 0.9743266763663136 2315 80-81 0.9841972721344554 2315 82-83 1.116091489967225 2315 84-85 1.0559170108532498 2315 86-87 1.1264839571398326 2315 88-89 1.2737902176149127 2315 90-91 1.3301227154988133 2315 92-93 1.403831778014947 2315 94-95 1.5090974681464324 2315 96-97 1.5156670735378341 2315 98-99 1.58025132537459 2315 100 1.6809332678712359 2316 1 0.11419591121502037 2316 2 0.030529114118493794 2316 3 0.1926985327443873 2316 4 0.10201836560271005 2316 5 0.13830666088835386 2316 6 0.2327705996563907 2316 7 0.24570288149193686 2316 8 0.2451711435338737 2316 9 0.403491363783715 2316 10-11 0.27875408484945297 2316 12-13 0.3419634143604995 2316 14-15 0.5614615276876975 2316 16-17 0.5955371210400102 2316 18-19 0.5598366430306925 2316 20-21 0.523914354198844 2316 22-23 0.3758985142111584 2316 24-25 0.47477194147517565 2316 26-27 0.5656869256331731 2316 28-29 0.65645614840831 2316 30-31 0.6775194440246537 2316 32-33 0.7691585197072826 2316 34-35 0.7540996267528186 2316 36-37 0.7751708718433363 2316 38-39 0.8473971422586288 2316 40-41 0.8708396523131157 2316 42-43 0.8373439832952769 2316 44-45 0.6789347702320043 2316 46-47 0.7338151823990486 2316 48-49 0.8077038985978717 2316 50-51 0.5823752609344126 2316 52-53 0.3743871484487613 2316 54-55 0.3564047591924009 2316 56-57 0.45813108015701687 2316 58-59 0.46928923314849413 2316 60-61 0.5147932844393424 2316 62-63 0.5740161801953576 2316 64-65 0.5930697745353513 2316 66-67 0.6235806858659672 2316 68-69 0.542815534658537 2316 70-71 0.4036491128937101 2316 72-73 0.5437152983563251 2316 74-75 0.6042143362536834 2316 76-77 0.6476231138729247 2316 78-79 0.712288877336757 2316 80-81 0.8113984842359123 2316 82-83 0.7889989151109091 2316 84-85 0.8098708431244148 2316 86-87 0.8758535178927644 2316 88-89 1.0357285867397188 2316 90-91 0.9694140862521152 2316 92-93 1.0017293985906335 2316 94-95 1.1314460596619327 2316 96-97 1.1496216894091162 2316 98-99 1.2506098400758034 2316 100 1.2941408641816352 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 43.0 1 533.0 2 264.0 3 178.0 4 159.0 5 116.0 6 190.0 7 1112.0 8 3864.0 9 6912.0 10 9611.0 11 12816.0 12 16753.0 13 20343.0 14 24010.0 15 28069.0 16 34382.0 17 43006.0 18 52609.0 19 62655.0 20 74583.0 21 88426.0 22 97159.0 23 100884.0 24 102392.0 25 106884.0 26 117896.0 27 134608.0 28 159071.0 29 187231.0 30 223601.0 31 275991.0 32 348302.0 33 449718.0 34 618493.0 35 834718.0 36 1216268.0 37 1758047.0 38 1628631.0 39 424594.0 40 527.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.4624080103829 18.503437621312937 12.502367444472002 23.53178692383216 2 16.021724203265205 20.49096811785431 40.24619524351533 23.241112435365157 3 18.57644318276643 27.11856996614995 29.9987013549197 24.306285496163923 4 12.39910630730839 16.698974166270585 38.374972891170565 32.52694663525047 5 12.83543519145155 37.73600630368515 34.31876813434907 15.109790370514231 6 29.52670390019017 37.02687856393681 19.30740074320909 14.139016792663927 7 26.957735840987834 32.84837043026144 23.050336494917236 17.143557233833487 8 26.358767484692258 34.47009029150369 20.735719826811703 18.435422396992358 9 27.656943895444915 15.57670719839789 20.62046922427689 36.14587968188031 10-11 25.128553464253283 25.59663605051107 28.971361199644104 20.303449285591544 12-13 25.63085603433602 23.877927959808506 28.421902077904086 22.069313927951388 14-15 23.043295008631194 25.229097858147888 26.56865341653406 25.15895371668686 16-17 22.19420853378161 27.570505088907527 27.28626806989501 22.94901830741586 18-19 21.875089898202717 27.37115229620452 28.852933745706004 21.900824059886762 20-21 23.102249149011346 26.553884829468167 28.334519152682685 22.009346868837802 22-23 22.759150027635762 26.339832415020037 28.375961854014093 22.52505570333011 24-25 22.460094716721525 26.25006976212223 28.687015868413468 22.602819652742774 26-27 22.318656085334602 26.32787491868942 28.680468253952835 22.673000742023145 28-29 22.07918798821879 27.352628292280627 28.425534372384 22.142649347116578 30-31 23.12045197515687 26.485298167140524 28.46472962308382 21.929520234618792 32-33 23.05320668314026 26.55955592547372 28.213613708274902 22.173623683111114 34-35 23.12503316181221 26.589606576626306 28.41900510407182 21.866355157489664 36-37 22.7408886548731 26.472279429247365 28.54073012746694 22.246101788412602 38-39 22.602578392134742 26.90402411608972 28.41608890784016 22.07730858393538 40-41 22.599962562414866 26.314190939024883 28.691418221654253 22.394428276905998 42-43 22.310926930343104 26.63295721023282 28.080816761049654 22.97529909837442 44-45 21.951222672232632 26.83747951848023 28.500585402215155 22.710712407071988 46-47 22.079187325734875 26.41031561547796 28.331436876715454 23.179060182071712 48-49 22.341019762674723 26.468043186897503 28.322116897996253 22.86882015243152 50-51 22.025554874114317 26.83116041622157 28.300736248018605 22.842548461645503 52-53 21.946485575139974 27.160408848780694 27.747428499241224 23.145677076838112 54-55 21.7494118167223 26.685740750847987 28.261209789930803 23.30363764249891 56-57 22.231314756526338 26.541870643589288 27.93648353116074 23.29033106872363 58-59 22.14279301212751 26.609366058983717 28.158649568336948 23.089191360551826 60-61 22.29923328268232 26.78450368266988 28.10588847190682 22.810374562740975 62-63 22.23427461267287 26.569383796409074 27.96142049968038 23.23492109123768 64-65 21.834496698822903 26.47642378004776 27.95741671003716 23.73166281109218 66-67 21.97196134350285 26.23445487671429 27.719867572261776 24.07371620752108 68-69 21.57429090689433 26.427734282956422 27.99396351343772 24.00401129671153 70-71 21.952570300426533 26.128415393617445 28.125261316922302 23.793752989033717 72-73 21.95418270104413 26.45530027119472 27.967110689490198 23.623406338270954 74-75 22.081560366566574 26.701102159967537 27.6654712157171 23.55186625774878 76-77 22.07793126031252 26.266956846186236 27.711890446382824 23.94322144711842 78-79 21.944125557198117 26.32462703685293 28.005269870363563 23.72597753558539 80-81 22.204972061864815 26.309587267784018 27.77467965761346 23.7107610127377 82-83 22.513532038007746 26.128962493287748 28.087721857713888 23.269783610990615 84-85 22.536917831292815 26.463660825375317 27.503343115772026 23.49607822755984 86-87 22.237418391873575 26.63436450687125 27.725331473309218 23.402885627945956 88-89 22.275401728449996 26.04572779066111 27.761089354631906 23.917781126256987 90-91 22.875228860451177 25.694882453083945 27.826926500078315 23.602962186386563 92-93 23.05980558088413 25.895369514090383 27.68605996609815 23.358764938927337 94-95 22.776272008882223 25.675721700803077 27.792520009424138 23.755486280890565 96-97 22.73319118597114 25.58544567613276 27.86695887785807 23.81440426003802 98-99 22.782581621759807 25.75521074972839 27.48077828156199 23.981429346949813 100 23.041205855900255 25.390300327185855 27.436769204045675 24.131724612868215 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1522.0 1 1138.0 2 2243.0 3 3473.5 4 2454.5 5 1818.0 6 2465.5 7 4353.5 8 6914.0 9 8161.5 10 7413.0 11 6716.5 12 7046.0 13 7691.5 14 8557.0 15 9420.5 16 10339.5 17 11256.5 18 12260.0 19 13291.0 20 14418.5 21 16259.5 22 18596.5 23 22169.5 24 27194.0 25 33039.0 26 40761.5 27 49983.0 28 60827.5 29 74619.0 30 91078.0 31 106587.5 32 125954.5 33 146842.5 34 165429.5 35 183752.5 36 204963.0 37 232187.5 38 248557.5 39 252693.0 40 256604.0 41 262200.0 42 271900.5 43 284917.5 44 300073.5 45 323629.0 46 350418.5 47 379370.0 48 417751.0 49 429778.5 50 414532.0 51 404252.0 52 388497.5 53 367579.5 54 345994.0 55 312786.0 56 277339.5 57 243750.5 58 209235.0 59 177661.0 60 143640.0 61 112176.0 62 88560.0 63 69210.0 64 51137.0 65 36200.0 66 26095.0 67 19508.5 68 14508.0 69 10758.5 70 8235.0 71 6148.0 72 4607.0 73 3482.0 74 2612.5 75 1914.0 76 1356.5 77 905.5 78 602.0 79 459.0 80 345.5 81 257.0 82 209.5 83 153.5 84 108.5 85 92.5 86 81.0 87 66.5 88 55.5 89 40.0 90 35.0 91 32.5 92 25.5 93 23.0 94 16.5 95 10.5 96 10.5 97 8.0 98 3.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22068610628354257 2 0.14548360292948717 3 0.02318240200983223 4 0.022254242525267254 5 0.02693821015667656 6 0.05394117562622974 7 0.06074048347827551 8 0.05371453203116155 9 0.04157291086679411 10-11 0.04355334418560427 12-13 0.04196683902012693 14-15 0.02015509113284995 16-17 0.018622548728103126 18-19 0.018331149820158307 20-21 0.021714614917962033 22-23 0.02470415186243295 24-25 0.021865710648007495 26-27 0.019286290685088546 28-29 0.018822210942806058 30-31 0.017268083433767026 32-33 0.02524377946973817 34-35 0.025454234236587204 36-37 0.024461319439145604 38-39 0.022923380758325725 40-41 0.024078183837958896 42-43 0.029879180616490003 44-45 0.039295682363966085 46-47 0.044017423927886754 48-49 0.04014289770743528 50-51 0.028082220684163623 52-53 0.043537155357385116 54-55 0.04174019542505873 56-57 0.044060594136471176 58-59 0.04783798738760771 60-61 0.024401960402342028 62-63 0.02576721824882423 64-65 0.026312242132202502 66-67 0.0316977256531086 68-69 0.035696366223240274 70-71 0.039581684995837856 72-73 0.04358032556596953 74-75 0.025238383193665115 76-77 0.01865492638454144 78-79 0.017958806771117705 80-81 0.021228950071387337 82-83 0.019431990139060953 84-85 0.011742296734961577 86-87 0.013523067839068802 88-89 0.01880062583851385 90-91 0.019599274697325576 92-93 0.019944636366000912 94-95 0.023759803549648816 96-97 0.024364186469830663 98-99 0.029728084886444545 100 0.02933415673311173 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9265649.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.17790018375242 #Duplication Level Percentage of deduplicated Percentage of total 1 76.29496144216944 45.912705741901114 2 14.199328832648215 17.08971586334764 3 4.48133334640343 8.090316924299602 4 1.855376578695948 4.466106662241472 5 0.9119877226858987 2.744075307229984 6 0.5408322762887037 1.9527690443191932 7 0.3669427308819005 1.5457290122515173 8 0.24115581058792834 1.1609800238633805 9 0.17435462676890431 0.9443065793647077 >10 0.8644799634442004 9.08774333934916 >50 0.04646762558124271 1.9210149005118056 >100 0.020295810044133406 2.169592133744525 >500 0.0015406096753486772 0.6113919036843892 >1k 8.276699667659541E-4 1.015463014958313 >5k 2.2990831639976768E-5 0.084036217873282 >10k+ 9.196332655990707E-5 1.204053331059946 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30812 0.33254011672576844 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23478 0.2533875392862389 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17013 0.183613689661674 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15724 0.16970208994534544 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.079255214610439E-5 0.0 2 0.0 0.0 0.0 1.079255214610439E-5 0.0 3 0.0 0.0 0.0 1.079255214610439E-5 0.0 4 0.0 0.0 0.0 1.079255214610439E-5 0.0 5 0.0 1.079255214610439E-5 0.0 2.158510429220878E-5 0.0 6 2.158510429220878E-5 1.079255214610439E-5 0.0 2.158510429220878E-5 0.0 7 2.158510429220878E-5 2.158510429220878E-5 0.0 3.237765643831317E-5 0.0 8 2.158510429220878E-5 2.158510429220878E-5 0.0 3.237765643831317E-5 0.0 9 2.158510429220878E-5 2.158510429220878E-5 0.0 3.237765643831317E-5 2.158510429220878E-5 10-11 2.158510429220878E-5 2.158510429220878E-5 0.0 4.856648465746975E-5 2.158510429220878E-5 12-13 2.158510429220878E-5 2.6981380365260974E-5 0.0 9.713296931493952E-5 3.237765643831317E-5 14-15 2.158510429220878E-5 3.7773932511365366E-5 0.0 1.0792552146104391E-4 6.475531287662634E-5 16-17 2.158510429220878E-5 4.317020858441756E-5 0.0 1.7268083433767025E-4 8.634041716883512E-5 18-19 2.158510429220878E-5 4.317020858441756E-5 0.0 2.266435950681922E-4 1.0792552146104391E-4 20-21 2.158510429220878E-5 4.317020858441756E-5 0.0 5.504201594513239E-4 1.187180736071483E-4 22-23 2.158510429220878E-5 4.317020858441756E-5 0.0 0.001435409435431884 1.241143496802005E-4 24-25 2.158510429220878E-5 4.317020858441756E-5 0.0 0.003561542208214449 1.4569945397240925E-4 26-27 2.158510429220878E-5 4.317020858441756E-5 0.0 0.0071068955882097416 1.5649200611851367E-4 28-29 2.158510429220878E-5 6.475531287662634E-5 0.0 0.018746663077783325 1.6188828219156586E-4 30-31 2.158510429220878E-5 7.554786502273073E-5 0.0 0.0604706696746229 1.7268083433767025E-4 32-33 2.158510429220878E-5 7.554786502273073E-5 0.0 0.15416621113102819 2.1585104292208782E-4 34-35 2.158510429220878E-5 8.634041716883512E-5 0.0 0.28704950942993845 2.266435950681922E-4 36-37 2.158510429220878E-5 9.713296931493952E-5 0.0 0.4883791734394428 2.266435950681922E-4 38-39 2.158510429220878E-5 1.0792552146104391E-4 0.0 0.8160626416994643 2.266435950681922E-4 40-41 2.158510429220878E-5 1.1871807360714829E-4 0.0 1.212947954320307 2.374361472142966E-4 42-43 2.158510429220878E-5 1.4030317789935707E-4 0.0 1.587174303710404 2.48228699360401E-4 44-45 2.158510429220878E-5 1.4030317789935707E-4 0.0 1.9903462779563523 2.5902125150650536E-4 46-47 2.158510429220878E-5 1.4030317789935707E-4 0.0 2.453222650674551 2.644175275795576E-4 48-49 2.158510429220878E-5 1.4569945397240925E-4 0.0 2.9432962548009316 3.0219146009092293E-4 50-51 2.158510429220878E-5 1.6188828219156586E-4 0.0 3.464684448979235 3.237765643831317E-4 52-53 2.158510429220878E-5 1.7268083433767025E-4 0.0 3.9869468398813726 3.453616686753405E-4 54-55 2.158510429220878E-5 1.7807711041072246E-4 0.0 4.560052943943808 3.5615422082144486E-4 56-57 2.158510429220878E-5 1.8347338648377464E-4 0.0 5.162379882941821 3.5615422082144486E-4 58-59 2.158510429220878E-5 1.8347338648377464E-4 0.0 5.841069524649596 3.5615422082144486E-4 60-61 2.158510429220878E-5 1.8347338648377464E-4 0.0 6.507617545193003 3.5615422082144486E-4 62-63 2.158510429220878E-5 1.8347338648377464E-4 0.0 7.197353364022315 3.5615422082144486E-4 64-65 2.158510429220878E-5 1.8347338648377464E-4 0.0 7.849315250340261 3.5615422082144486E-4 66-67 2.158510429220878E-5 2.104547668490356E-4 0.0 8.544128964954318 3.5615422082144486E-4 68-69 2.158510429220878E-5 2.374361472142966E-4 0.0 9.310286845530195 3.5615422082144486E-4 70-71 3.237765643831317E-5 2.48228699360401E-4 0.0 10.10131616252677 3.669467729675493E-4 72-73 3.237765643831317E-5 2.48228699360401E-4 0.0 10.837206330608897 3.9932442940586243E-4 74-75 3.237765643831317E-5 2.8060635579871414E-4 0.0 11.536099629934178 4.209095336980712E-4 76-77 3.237765643831317E-5 2.8060635579871414E-4 0.0 12.27153651082617 4.370983619172278E-4 78-79 3.237765643831317E-5 2.8060635579871414E-4 0.0 13.072181991784925 4.4249463799028E-4 80-81 3.237765643831317E-5 2.8060635579871414E-4 0.0 13.861851447211091 4.586834662094366E-4 82-83 3.237765643831317E-5 2.9139890794481856E-4 0.0 14.659275351354232 4.856648465746976E-4 84-85 3.237765643831317E-5 2.9139890794481856E-4 0.0 15.443688833885247 4.96457398720802E-4 86-87 3.237765643831317E-5 2.9139890794481856E-4 0.0 16.218599474251615 4.96457398720802E-4 88 3.237765643831317E-5 2.9139890794481856E-4 0.0 16.81275645127503 4.96457398720802E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 10410 0.0 51.135838 2 ACCTGGG 12325 0.0 42.680187 3 GTACCTG 15520 0.0 34.97106 1 CCTGGGG 11880 0.0 32.526413 4 TAACGCA 1160 0.0 30.393736 4 GTACATG 33175 0.0 29.638731 1 TACATGG 34055 0.0 28.194395 2 ATAACGC 1260 0.0 27.608599 3 ACATGGG 34345 0.0 27.046219 3 TGGGGGG 20885 0.0 26.627687 6 CTGGGGG 8495 0.0 26.00864 5 CATGGGG 25735 0.0 25.975042 4 GTATCAA 46370 0.0 25.563578 1 ATGGGGG 19315 0.0 25.360405 5 GGTATCA 33585 0.0 22.894062 1 CAACGCA 52855 0.0 21.914814 5 ATCAACG 53080 0.0 21.892769 3 TATAACG 1830 0.0 21.604374 2 TATCAAC 54105 0.0 21.547758 2 TCAACGC 54130 0.0 21.459301 4 >>END_MODULE