FastQCFastQC Report
Mon 6 Jun 2016
ERR1211432_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211432_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9265649
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274110.29583464687686745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223930.2416776202077156No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT170830.1843691683119013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156640.16905453681657917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG105050.049.923392
ACCTGGG122400.041.733883
CCTGGGG111600.033.9824454
GTACCTG164850.032.087011
CTGGGGG77050.028.479255
GTACATG319600.027.9666881
TGGGGGG183400.027.592376
TACATGG327000.026.6439042
ACATGGG324200.025.67123
GTATCAA449400.024.5134771
ATAACGC11550.024.4123653
CATGGGG244100.024.2191074
ATGGGGG175050.024.2118935
TAACGCA11000.023.9243764
GGTATCA330850.023.1502461
GTACTAG31150.022.0374071
CAACGCA514850.021.0365525
ATCAACG516550.020.915453
AGAGTAC407750.020.7474510-11
TCAACGC526350.020.5887494
Produced by FastQC (version 0.11.5)