##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211432_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9265649 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07568072133965 33.0 31.0 34.0 30.0 34.0 2 32.20982124403806 33.0 31.0 34.0 30.0 34.0 3 32.28991903319454 34.0 31.0 34.0 30.0 34.0 4 35.93290281123319 37.0 35.0 37.0 35.0 37.0 5 35.79323779694223 37.0 35.0 37.0 35.0 37.0 6 35.82935464099708 37.0 35.0 37.0 35.0 37.0 7 35.76012700243663 37.0 35.0 37.0 33.0 37.0 8 35.743614613504135 37.0 35.0 37.0 33.0 37.0 9 37.33229339898371 39.0 37.0 39.0 34.0 39.0 10-11 37.30666367784923 39.0 37.0 39.0 34.0 39.0 12-13 37.218347252307964 39.0 37.0 39.0 33.0 39.0 14-15 38.42313587531753 40.0 38.0 41.0 33.5 41.0 16-17 38.418179179893386 40.0 38.0 41.0 34.0 41.0 18-19 38.426153364972066 40.0 38.0 41.0 34.0 41.0 20-21 38.382925200382616 40.0 38.0 41.0 34.0 41.0 22-23 38.27923807603763 40.0 38.0 41.0 34.0 41.0 24-25 38.158146828139074 40.0 37.5 41.0 33.0 41.0 26-27 38.03612903963878 40.0 37.0 41.0 33.0 41.0 28-29 37.96616696790478 40.0 37.0 41.0 33.0 41.0 30-31 38.01601555379445 40.0 37.0 41.0 33.0 41.0 32-33 37.860349231877876 40.0 37.0 41.0 33.0 41.0 34-35 37.751574282600174 40.0 37.0 41.0 33.0 41.0 36-37 37.61123878100714 40.0 37.0 41.0 32.5 41.0 38-39 37.50002142321601 40.0 36.0 41.0 32.0 41.0 40-41 37.44290777688643 40.0 36.0 41.0 32.0 41.0 42-43 37.3159532052207 40.0 36.0 41.0 31.5 41.0 44-45 37.16634576811619 40.0 36.0 41.0 31.0 41.0 46-47 36.9542824792953 39.0 36.0 41.0 31.0 41.0 48-49 36.74073667154886 39.0 35.0 41.0 30.0 41.0 50-51 36.56163637323192 39.0 35.0 41.0 30.0 41.0 52-53 36.36106440034584 39.0 35.0 40.0 29.5 41.0 54-55 36.164270468264014 39.0 35.0 40.0 29.0 41.0 56-57 35.93528461956632 38.0 35.0 40.0 28.5 41.0 58-59 35.675029941237796 38.0 34.0 40.0 28.0 41.0 60-61 35.408247657557496 38.0 34.0 40.0 28.0 41.0 62-63 35.11522479429126 37.0 34.0 40.0 27.5 41.0 64-65 34.763661077599636 36.5 34.0 39.5 26.5 41.0 66-67 34.40325210894564 36.0 33.0 39.0 26.0 40.5 68-69 34.03324499989154 36.0 33.0 39.0 26.0 40.0 70-71 33.650938374635174 35.0 33.0 38.0 26.0 40.0 72-73 33.29037113320395 35.0 32.0 37.5 25.5 40.0 74-75 32.92204944305574 35.0 32.0 37.0 25.0 39.0 76-77 31.910986105776292 34.0 30.5 36.0 23.5 38.5 78-79 32.4033869618847 35.0 32.0 36.0 25.0 38.0 80-81 32.30299248331121 35.0 32.0 35.5 25.0 37.0 82-83 32.03768046900978 34.5 31.5 35.0 24.5 37.0 84-85 31.76589653892566 34.0 31.0 35.0 24.0 36.5 86-87 31.52481418193156 34.0 31.0 35.0 23.5 36.0 88-89 31.304452715616577 34.0 31.0 35.0 23.0 36.0 90-91 31.086794513800385 34.0 31.0 35.0 22.5 35.0 92-93 30.909399654573576 34.0 30.5 35.0 20.5 35.0 94-95 30.741423347679152 34.0 30.0 35.0 20.0 35.0 96-97 30.574033130329024 34.0 30.0 35.0 20.0 35.0 98-99 30.388062077464838 34.0 29.5 35.0 19.5 35.0 100 28.56339291505646 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.26267090670866367 1101 2 -0.2731959997002775 1101 3 -0.28867445001557 1101 4 -0.13064213396133795 1101 5 -0.15517662181527925 1101 6 -0.2290070201271064 1101 7 -0.2437982452766576 1101 8 -0.30672181771225837 1101 9 -0.38777188822923847 1101 10-11 -0.4011056624770504 1101 12-13 -0.44159751819788795 1101 14-15 -0.5713923366761264 1101 16-17 -0.5570432037189263 1101 18-19 -0.5621471832350622 1101 20-21 -0.6024354366641731 1101 22-23 -0.6286568403096098 1101 24-25 -0.6406505273121823 1101 26-27 -0.6407166740181296 1101 28-29 -0.691187292823706 1101 30-31 -0.6279063814281614 1101 32-33 -0.5071165336114589 1101 34-35 -0.6393586566108098 1101 36-37 -0.6413105297159021 1101 38-39 -0.5611446920163985 1101 40-41 -0.6397821935286245 1101 42-43 -0.5663332672192709 1101 44-45 -0.582701041745068 1101 46-47 -0.649357795618215 1101 48-49 -0.7078983431330315 1101 50-51 -0.7333025611583466 1101 52-53 -0.7691493434092607 1101 54-55 -0.7156364103475639 1101 56-57 -0.6653120898136322 1101 58-59 -0.7074652934421266 1101 60-61 -0.6729335618011234 1101 62-63 -0.7600130846401214 1101 64-65 -0.7260148027636077 1101 66-67 -0.7642837186983442 1101 68-69 -0.6944666883452868 1101 70-71 -0.7398193765832488 1101 72-73 -0.7938294318388586 1101 74-75 -0.9051816911230475 1101 76-77 -0.6860350892312432 1101 78-79 -0.6323171340742917 1101 80-81 -0.5667544669420224 1101 82-83 -0.6760785922343722 1101 84-85 -0.7049604197416066 1101 86-87 -0.7833580380686982 1101 88-89 -0.8288283336099482 1101 90-91 -0.7452038483197967 1101 92-93 -0.9832779803914597 1101 94-95 -1.121946005171985 1101 96-97 -1.0597946278659478 1101 98-99 -1.1756903466842665 1101 100 -1.144568481040018 1102 1 -0.30334035661834235 1102 2 -0.27169117712933044 1102 3 -0.2937972543128353 1102 4 -0.1592543089250924 1102 5 -0.17948864043928836 1102 6 -0.2647858681637203 1102 7 -0.26367868866810085 1102 8 -0.34860037045257997 1102 9 -0.45738383037785724 1102 10-11 -0.43475843004360826 1102 12-13 -0.4547397546819454 1102 14-15 -0.6175460609169363 1102 16-17 -0.6270170516043407 1102 18-19 -0.636684746878835 1102 20-21 -0.6510783521027221 1102 22-23 -0.6552588453953803 1102 24-25 -0.738436756183475 1102 26-27 -0.7192296183229487 1102 28-29 -0.7775334471647355 1102 30-31 -0.7423039610766651 1102 32-33 -0.6950468710869231 1102 34-35 -0.7510725581712876 1102 36-37 -0.6813495106944671 1102 38-39 -0.7809408772712345 1102 40-41 -0.7905515516152732 1102 42-43 -0.7898604786842398 1102 44-45 -0.7823878693797255 1102 46-47 -0.827479180517912 1102 48-49 -0.8617461738070062 1102 50-51 -0.893193414309458 1102 52-53 -0.8842719803693768 1102 54-55 -0.9274578025411984 1102 56-57 -0.9573874202885051 1102 58-59 -1.0251328806809852 1102 60-61 -0.9916548284701108 1102 62-63 -1.0760832215906717 1102 64-65 -1.002936011296221 1102 66-67 -1.0062703897563523 1102 68-69 -1.0516837375257566 1102 70-71 -1.0920448088921049 1102 72-73 -1.14438791924389 1102 74-75 -1.1690117289152369 1102 76-77 -0.9320046772492212 1102 78-79 -0.8527334062766379 1102 80-81 -0.8225304001261051 1102 82-83 -0.8335619961400056 1102 84-85 -0.8917402972761366 1102 86-87 -0.9244765288526082 1102 88-89 -0.8782364046443369 1102 90-91 -0.950774823447027 1102 92-93 -1.0266214208070927 1102 94-95 -1.0137725642576108 1102 96-97 -1.0519915766411323 1102 98-99 -1.1828253564090865 1102 100 -1.3613134455635212 1103 1 -0.3850998171683031 1103 2 -0.3315113582462601 1103 3 -0.3989302480924266 1103 4 -0.23067236356901333 1103 5 -0.18993411865243814 1103 6 -0.3742109669016145 1103 7 -0.3916666439816936 1103 8 -0.44702777029127816 1103 9 -0.5261789159290373 1103 10-11 -0.5491409860765444 1103 12-13 -0.5982019637403724 1103 14-15 -0.8153470388138828 1103 16-17 -0.8675995885988783 1103 18-19 -0.7739939888383986 1103 20-21 -0.7170591622101483 1103 22-23 -0.8617842671663354 1103 24-25 -0.8309230853173588 1103 26-27 -0.8691615135373496 1103 28-29 -0.8725641369990527 1103 30-31 -0.8352626633843485 1103 32-33 -0.8322849806778265 1103 34-35 -0.7872470402473724 1103 36-37 -0.7678920361756596 1103 38-39 -0.9017313355630563 1103 40-41 -0.9152792446820683 1103 42-43 -0.9666691470276376 1103 44-45 -0.8521035797436411 1103 46-47 -0.9079273630414946 1103 48-49 -0.8896009567700531 1103 50-51 -1.0470024332136632 1103 52-53 -1.0321625493917068 1103 54-55 -1.0641131953274865 1103 56-57 -1.0720510087242374 1103 58-59 -1.1585117787492365 1103 60-61 -1.1237475338068137 1103 62-63 -1.2087462318805677 1103 64-65 -1.1341420850105735 1103 66-67 -1.1395274272623368 1103 68-69 -1.2241532281210752 1103 70-71 -1.165937716491733 1103 72-73 -1.1954439908806656 1103 74-75 -1.1936531572052864 1103 76-77 -1.1811745839162278 1103 78-79 -0.9856487633426809 1103 80-81 -0.9356148403400439 1103 82-83 -0.8986594101049477 1103 84-85 -0.9235131430750698 1103 86-87 -0.9091539571566472 1103 88-89 -0.9469377094679245 1103 90-91 -1.1340481526266473 1103 92-93 -1.1729500309548193 1103 94-95 -1.1539417983027782 1103 96-97 -1.2797664047015367 1103 98-99 -1.2889207058074525 1103 100 -1.3692524319584543 1104 1 -0.370102861677168 1104 2 -0.30684292825863935 1104 3 -0.35044296741205017 1104 4 -0.2107810872024345 1104 5 -0.22357155505927295 1104 6 -0.28967674243447306 1104 7 -0.2721793022051955 1104 8 -0.300950601087294 1104 9 -0.5373423164703937 1104 10-11 -0.43855451138440316 1104 12-13 -0.5127354948695242 1104 14-15 -0.6484991249031111 1104 16-17 -0.7048653587772478 1104 18-19 -0.7335272350811621 1104 20-21 -0.6841516802695438 1104 22-23 -0.7084899918727174 1104 24-25 -0.8393075185753318 1104 26-27 -0.7887298660221447 1104 28-29 -0.8347866284713419 1104 30-31 -0.7636896923075227 1104 32-33 -0.7209722722044845 1104 34-35 -0.74953118388229 1104 36-37 -0.7139980441208991 1104 38-39 -0.8237921694495682 1104 40-41 -0.8455707555479037 1104 42-43 -0.7109898165312742 1104 44-45 -0.7739188072305865 1104 46-47 -0.747794571655902 1104 48-49 -0.8636348452085443 1104 50-51 -0.8000976312646273 1104 52-53 -0.7717910232037823 1104 54-55 -0.7875467560436462 1104 56-57 -0.8457340818727701 1104 58-59 -0.9369459058058283 1104 60-61 -0.9840840841826193 1104 62-63 -0.9579600552551213 1104 64-65 -0.9991814302249367 1104 66-67 -0.9521590979803349 1104 68-69 -0.8695346086325131 1104 70-71 -0.881769093948364 1104 72-73 -0.8033944426143833 1104 74-75 -0.7906386472201916 1104 76-77 -0.7744039806229388 1104 78-79 -0.7776260602993368 1104 80-81 -0.787107267510379 1104 82-83 -0.8228055163257011 1104 84-85 -0.7286835017092912 1104 86-87 -0.7207183541825692 1104 88-89 -0.7456392521792594 1104 90-91 -0.726188116284753 1104 92-93 -0.8938252157019306 1104 94-95 -0.9726537685427878 1104 96-97 -0.9743008900285126 1104 98-99 -0.9176395888330973 1104 100 -0.9759491981889106 1105 1 -0.4047789113443798 1105 2 -0.40190376822545204 1105 3 -0.3848182392255879 1105 4 -0.22723237873014313 1105 5 -0.2939018507067246 1105 6 -0.3414515216336298 1105 7 -0.295068125369518 1105 8 -0.3269234082524477 1105 9 -0.5156871060982766 1105 10-11 -0.5244088087029226 1105 12-13 -0.5495646402037906 1105 14-15 -0.7326934344427514 1105 16-17 -0.7581141564574949 1105 18-19 -0.7080932398907791 1105 20-21 -0.7854257193819052 1105 22-23 -0.7181770717290945 1105 24-25 -0.7293729525979415 1105 26-27 -0.8202618885273623 1105 28-29 -0.7293984538896794 1105 30-31 -0.7481773771193119 1105 32-33 -0.6792576492614799 1105 34-35 -0.6616244591510565 1105 36-37 -0.7563941226718853 1105 38-39 -0.8025809357579519 1105 40-41 -0.7503065296540186 1105 42-43 -0.8559624184118704 1105 44-45 -0.833215507152886 1105 46-47 -0.9198981831218163 1105 48-49 -0.8076407521944162 1105 50-51 -0.813127189155729 1105 52-53 -0.8789874359676091 1105 54-55 -0.8087929669329057 1105 56-57 -0.864624619968474 1105 58-59 -0.8535239391249831 1105 60-61 -0.981087265579113 1105 62-63 -0.9457879287847604 1105 64-65 -0.8966147632955241 1105 66-67 -0.8771619613399864 1105 68-69 -0.9504576214420624 1105 70-71 -0.9381765594518257 1105 72-73 -1.0785834774719802 1105 74-75 -1.062943019438439 1105 76-77 -0.9540293962898865 1105 78-79 -0.8080545224027489 1105 80-81 -0.7626441688651582 1105 82-83 -0.7961732762325653 1105 84-85 -0.6859163139260147 1105 86-87 -0.7148254537723062 1105 88-89 -0.7627869004373764 1105 90-91 -0.7331724793594461 1105 92-93 -0.7954686847572567 1105 94-95 -0.8238903183777566 1105 96-97 -0.8454004167088236 1105 98-99 -0.8170412477653244 1105 100 -0.9066581153180415 1106 1 -0.37496386290187544 1106 2 -0.3658927781507977 1106 3 -0.4191458080809767 1106 4 -0.24363802684499092 1106 5 -0.2622344708363116 1106 6 -0.31991402933011415 1106 7 -0.36484856640583274 1106 8 -0.3846084635950149 1106 9 -0.5090397949838348 1106 10-11 -0.5169418139953095 1106 12-13 -0.5588210739498081 1106 14-15 -0.6458776703202176 1106 16-17 -0.6978223409593909 1106 18-19 -0.6831177509210562 1106 20-21 -0.6944169904562827 1106 22-23 -0.6935689136432259 1106 24-25 -0.75016086402929 1106 26-27 -0.7741179178174775 1106 28-29 -0.7301716117264547 1106 30-31 -0.8083365798729574 1106 32-33 -0.7232531323711697 1106 34-35 -0.7520871898215802 1106 36-37 -0.8101724415219209 1106 38-39 -0.7294778019075494 1106 40-41 -0.7476917671230083 1106 42-43 -0.7234324084113624 1106 44-45 -0.8086233196781478 1106 46-47 -0.8341791268056369 1106 48-49 -0.8279870513060601 1106 50-51 -0.8783288921704795 1106 52-53 -0.9294993858903311 1106 54-55 -0.9010578366575146 1106 56-57 -0.8937893115323376 1106 58-59 -0.9985389726661396 1106 60-61 -1.081223202177135 1106 62-63 -0.8876144007957834 1106 64-65 -0.9514763934837376 1106 66-67 -0.8478264682210508 1106 68-69 -0.8630271560763347 1106 70-71 -0.8308753900780701 1106 72-73 -0.8198444396339042 1106 74-75 -0.822612531436647 1106 76-77 -0.8513298181379767 1106 78-79 -0.8530258037070553 1106 80-81 -0.7848864369317354 1106 82-83 -0.7516106213469946 1106 84-85 -0.7344588669536343 1106 86-87 -0.7383879977170906 1106 88-89 -0.7229681793688307 1106 90-91 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0.9769576795287946 2311 38-39 0.9532793620592273 2311 40-41 1.0228191047893276 2311 42-43 1.0915890633773486 2311 44-45 1.0608324496014703 2311 46-47 1.0793406481983894 2311 48-49 1.1011940463220427 2311 50-51 1.0799665219592995 2311 52-53 1.0498915710835277 2311 54-55 1.0824246207270463 2311 56-57 1.1333337704552804 2311 58-59 1.1240729748997254 2311 60-61 1.1410861609411356 2311 62-63 1.160820767845351 2311 64-65 1.1642848362035423 2311 66-67 1.2606031432650084 2311 68-69 1.1958613017654969 2311 70-71 1.1887333068166939 2311 72-73 1.2343296453937356 2311 74-75 1.1038781780585651 2311 76-77 1.0079518904596334 2311 78-79 0.9920132604468535 2311 80-81 0.996293251825243 2311 82-83 1.0227859086595288 2311 84-85 1.0363156681506496 2311 86-87 1.104850225348173 2311 88-89 1.1150737385165286 2311 90-91 1.1276700426638229 2311 92-93 1.1695948285279307 2311 94-95 1.143672629247046 2311 96-97 1.2330162965272002 2311 98-99 1.2386565254473005 2311 100 1.5251868318820812 2312 1 0.12315951064047681 2312 2 0.1534019526775623 2312 3 0.32264533958043273 2312 4 0.25442632322143055 2312 5 0.3378056574963466 2312 6 0.41625530447554837 2312 7 0.46665478834058405 2312 8 0.2988018634550258 2312 9 0.4308939880739757 2312 10-11 0.27231335488835384 2312 12-13 0.4848321069884278 2312 14-15 0.8111546276140942 2312 16-17 0.8645870677027432 2312 18-19 0.9009500090467739 2312 20-21 0.8876607700745467 2312 22-23 0.48878760218392614 2312 24-25 0.5078912936459901 2312 26-27 0.8097604985702631 2312 28-29 0.9329289057723855 2312 30-31 0.8663457394365608 2312 32-33 0.7277899361339664 2312 34-35 0.8963731421631707 2312 36-37 0.9702849597866745 2312 38-39 0.9675572397864087 2312 40-41 1.001802728442378 2312 42-43 0.8906293883730498 2312 44-45 0.7966975285030813 2312 46-47 1.0013009094601557 2312 48-49 1.0567990812782355 2312 50-51 1.0954868249428173 2312 52-53 1.1745438992073218 2312 54-55 1.1233006028537815 2312 56-57 1.1006325990417807 2312 58-59 1.1767456584813374 2312 60-61 1.1802049043675638 2312 62-63 1.1871410306782266 2312 64-65 1.249501546765046 2312 66-67 1.2655586253126643 2312 68-69 1.288257723205021 2312 70-71 1.1352510434430698 2312 72-73 1.2316268135062387 2312 74-75 1.2993287245083067 2312 76-77 1.2477067097308598 2312 78-79 1.093939780211798 2312 80-81 0.9673139071659094 2312 82-83 1.0062820065456108 2312 84-85 0.8543023880993417 2312 86-87 1.0426360300203186 2312 88-89 0.6608780449007909 2312 90-91 1.0227781657068178 2312 92-93 1.1752692812729002 2312 94-95 1.232523974012782 2312 96-97 1.2755040398806834 2312 98-99 1.3065870158025241 2312 100 1.4108986213929988 2313 1 -0.35563854611027423 2313 2 0.01855251320176876 2313 3 0.2754363251637457 2313 4 0.11271679372147503 2313 5 0.2525630003021675 2313 6 0.37507222925790273 2313 7 0.12591652375684248 2313 8 0.1415748011881206 2313 9 0.3128272647296271 2313 10-11 0.41141556252146927 2313 12-13 0.6020578056534944 2313 14-15 0.7003087730873006 2313 16-17 0.8437126098122931 2313 18-19 0.7170507665758876 2313 20-21 0.9112041352946036 2313 22-23 0.9368278511705199 2313 24-25 0.7885569634313399 2313 26-27 0.9816041341725921 2313 28-29 0.8937505568591817 2313 30-31 0.8778133361250227 2313 32-33 1.0152826488835913 2313 34-35 1.0195931701531435 2313 36-37 0.893272104554292 2313 38-39 0.6577212201352722 2313 40-41 0.8233688980883258 2313 42-43 0.8615975873203539 2313 44-45 1.0513453194841063 2313 46-47 1.1031099165233798 2313 48-49 1.1522862777640128 2313 50-51 1.0316799493328261 2313 52-53 1.0290262586103793 2313 54-55 1.0991190655645013 2313 56-57 1.1315953494947024 2313 58-59 1.115230285221486 2313 60-61 1.0900812276347835 2313 62-63 1.2146605263288492 2313 64-65 1.2812486310785474 2313 66-67 1.281638319447346 2313 68-69 1.2393558846428476 2313 70-71 1.1275754162024398 2313 72-73 1.2383601560654824 2313 74-75 0.8370373867364691 2313 76-77 1.0618615588091451 2313 78-79 0.8980931304285349 2313 80-81 0.9752878120445345 2313 82-83 1.0301062430310282 2313 84-85 1.0337718709339612 2313 86-87 1.131634123400417 2313 88-89 1.116229980525972 2313 90-91 1.1956294943673882 2313 92-93 0.9639861929555131 2313 94-95 0.969370293580031 2313 96-97 1.2084037381365533 2313 98-99 1.3714475826274395 2313 100 1.3727721364156835 2314 1 -0.32058291823852514 2314 2 -0.1221270987582912 2314 3 0.15791990440074954 2314 4 -0.14073244337744484 2314 5 0.20547566678781948 2314 6 0.22772053488829158 2314 7 0.1622927785695083 2314 8 0.16765932945557438 2314 9 0.2544514676061027 2314 10-11 0.429982387220889 2314 12-13 0.5225372362548413 2314 14-15 0.622591746634285 2314 16-17 0.8023348011621678 2314 18-19 0.8302054171856668 2314 20-21 0.4841407016890784 2314 22-23 0.6763432501044946 2314 24-25 0.8458262982834484 2314 26-27 0.8181095034352595 2314 28-29 0.7530191298771527 2314 30-31 0.73607582697921 2314 32-33 0.8410583228283954 2314 34-35 0.8723769674670052 2314 36-37 0.7979703228288386 2314 38-39 0.7944692308676622 2314 40-41 0.8572106808880875 2314 42-43 0.6960211700354009 2314 44-45 0.7007922293833673 2314 46-47 0.8416688898672788 2314 48-49 0.6980181180398191 2314 50-51 0.7317335426218179 2314 52-53 0.9250700069225601 2314 54-55 0.9503534036309702 2314 56-57 0.7937873444478569 2314 58-59 0.8286948448502187 2314 60-61 0.8817127398019338 2314 62-63 0.6484010582833264 2314 64-65 0.7389532366429776 2314 66-67 0.8683649419197863 2314 68-69 0.9639455035369267 2314 70-71 1.0009311017390985 2314 72-73 1.051206516492492 2314 74-75 1.0539159983048165 2314 76-77 0.8131170643851782 2314 78-79 0.78914845955142 2314 80-81 0.8178061030538828 2314 82-83 0.834684017440317 2314 84-85 0.7124390929105004 2314 86-87 0.8350568091330501 2314 88-89 0.9241652457195499 2314 90-91 1.029491083382652 2314 92-93 1.0461394069708057 2314 94-95 1.1059115961005368 2314 96-97 1.0858527736167822 2314 98-99 1.0936324326263218 2314 100 1.1745767217937164 2315 1 -0.053419788161019 2315 2 0.11506178402431999 2315 3 0.11827882548178792 2315 4 -0.06906401719180622 2315 5 0.23865678034064075 2315 6 0.3757289761016054 2315 7 0.4489321884841715 2315 8 0.40250644880597264 2315 9 0.6226980311867862 2315 10-11 0.5995303711543727 2315 12-13 0.6573584725653987 2315 14-15 0.7494813824558406 2315 16-17 0.7115901868054664 2315 18-19 0.7706004585805388 2315 20-21 0.739612437881334 2315 22-23 0.8366437444409698 2315 24-25 0.796679527054728 2315 26-27 0.8763015894939272 2315 28-29 0.8941642836757424 2315 30-31 0.878985809092967 2315 32-33 0.8811652245005845 2315 34-35 0.930714772561231 2315 36-37 0.969327716754556 2315 38-39 0.9730753072331026 2315 40-41 0.9545499691596646 2315 42-43 1.0215633765417635 2315 44-45 0.9345148797778577 2315 46-47 0.9691645342482218 2315 48-49 1.0963613356239037 2315 50-51 0.9676809350434823 2315 52-53 1.0297461814684326 2315 54-55 0.986245443768631 2315 56-57 1.0832414289986403 2315 58-59 1.1099395040244744 2315 60-61 1.1823075339765907 2315 62-63 1.1362171310193858 2315 64-65 1.19139247926541 2315 66-67 1.2266732018078912 2315 68-69 1.123566486118527 2315 70-71 0.662059241656884 2315 72-73 0.928570110993455 2315 74-75 1.1006205923804728 2315 76-77 1.0415263472890395 2315 78-79 0.9598665176053913 2315 80-81 0.9496452645489413 2315 82-83 0.9467626174437243 2315 84-85 0.9330952139775519 2315 86-87 0.9167051501757228 2315 88-89 1.0102139993876094 2315 90-91 1.0657960567563833 2315 92-93 1.1060146102590132 2315 94-95 1.1673577967589424 2315 96-97 1.2380536395991655 2315 98-99 1.1616372692445296 2315 100 1.0723792461137407 2316 1 0.5128145971287879 2316 2 0.5109668853805474 2316 3 0.5436236987546152 2316 4 0.33479393027398885 2316 5 0.42424771123375393 2316 6 0.4306086216763205 2316 7 0.47876603300711906 2316 8 0.5190421316193294 2316 9 0.560893551941426 2316 10-11 0.5131743001234668 2316 12-13 0.6514939043580696 2316 14-15 0.772544903256744 2316 16-17 0.7879862935233746 2316 18-19 0.8453518172430918 2316 20-21 0.891259114480107 2316 22-23 0.9232873320512383 2316 24-25 0.985011617501236 2316 26-27 1.0227840922242848 2316 28-29 0.9652661150094914 2316 30-31 0.8309183380775167 2316 32-33 0.8124205425889315 2316 34-35 0.6921525833377729 2316 36-37 0.6380038262373162 2316 38-39 0.6101209576874567 2316 40-41 0.7019730208429067 2316 42-43 0.8686560205898033 2316 44-45 0.8088871904330261 2316 46-47 0.8115344218187914 2316 48-49 0.5463647901336799 2316 50-51 0.6289636439151778 2316 52-53 0.8235693711852505 2316 54-55 0.8764494794466628 2316 56-57 0.9061815792479067 2316 58-59 0.8547068861926022 2316 60-61 0.9791784236439369 2316 62-63 1.0108041195230228 2316 64-65 0.9977301821598772 2316 66-67 0.9252096142268229 2316 68-69 0.9418559142479594 2316 70-71 0.8609291253578633 2316 72-73 0.8947095076121556 2316 74-75 0.9308391530088969 2316 76-77 0.8017267313468146 2316 78-79 0.7674720572130553 2316 80-81 0.7364846943976247 2316 82-83 0.7192286275392945 2316 84-85 0.7082446366558415 2316 86-87 0.7229521307010742 2316 88-89 0.7192532655861683 2316 90-91 0.7636683145845353 2316 92-93 0.7947997580802095 2316 94-95 0.737214227926497 2316 96-97 0.9098418965103896 2316 98-99 0.9539201818100871 2316 100 0.6270388956052066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 5.0 11 6.0 12 44.0 13 228.0 14 987.0 15 2908.0 16 6776.0 17 13438.0 18 22170.0 19 32096.0 20 43655.0 21 56898.0 22 69327.0 23 75130.0 24 78679.0 25 85900.0 26 100839.0 27 122566.0 28 149749.0 29 182639.0 30 223547.0 31 276474.0 32 349418.0 33 453147.0 34 632321.0 35 868366.0 36 1314903.0 37 2030459.0 38 1799738.0 39 273190.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22596472905608 19.442243456803883 13.337326425219581 23.994465388920457 2 15.662810020107603 20.908173836500822 39.90524570917806 23.52377043421351 3 18.0480395922617 27.066522809141595 30.734533544277365 24.150904054319348 4 12.281011755615914 17.153352785162497 38.339205546307944 32.226429912913645 5 12.612746212256123 37.940038064697035 34.42595030607824 15.021265416968602 6 29.564577721431064 37.43103154457934 18.985728900371683 14.018661833617916 7 26.784914478525252 32.94986221114472 22.91728589088314 17.347937419446886 8 26.31267041500533 34.8872541232013 20.524539329063497 18.27553613272987 9 27.313698563310563 15.554378904551827 20.49625555881257 36.63566697332504 10-11 25.052214868826063 25.784118822823444 28.927226201114664 20.23644010723583 12-13 25.485735248003145 23.917340218572562 28.449050648912426 22.147873884511863 14-15 22.90281756933084 25.343338176312802 26.484812823628275 25.269031430728088 16-17 22.113857104643763 27.735735052530906 27.211977412678596 22.938430430146735 18-19 21.85981251825965 27.422795748036645 28.78349374123712 21.933897992466584 20-21 22.970097559820015 26.60580638257538 28.25185954334443 22.172236514260174 22-23 22.603794143701553 26.41892396516682 28.342615648684262 22.63466624244737 24-25 22.389066792234495 26.33601359344408 28.605030433397875 22.669889180923548 26-27 22.336417017307692 26.404043580757268 28.597171120986776 22.662368280948264 28-29 22.024556625772775 27.38574643779124 28.394395182290292 22.195301754145685 30-31 23.017778559884682 26.533725041539537 28.46318529441873 21.985311104157052 32-33 22.98017421835593 26.559329412281553 28.219440304021298 22.24105606534122 34-35 23.011167240458384 26.61180021771412 28.449200325923485 21.92783221590401 36-37 22.651172235817807 26.52031586088544 28.576770207578434 22.25174169571832 38-39 22.54510840601187 26.867164543387933 28.4526582625007 22.135068788099492 40-41 22.50653624926948 26.31898407394636 28.793655264857726 22.380824411926437 42-43 22.246740430672034 26.6338076744145 28.184153396983056 22.935298497930404 44-45 21.854926055323464 26.829350297885078 28.54289177650661 22.77283187028484 46-47 22.033252856353503 26.38826028429203 28.34540847833096 23.23307838102351 48-49 22.217503206112738 26.476139419331485 28.345922133284056 22.96043524127172 50-51 21.885207287661025 26.782616786225656 28.323194551864134 23.008981374249185 52-53 21.829020445648027 27.093554610169157 27.805226414190066 23.27219852999275 54-55 21.64417350215255 26.640237326537218 28.273441238567433 23.442147932742802 56-57 22.11510457097851 26.51450847957027 27.81740477903848 23.552982170412744 58-59 21.854064570674296 26.617886713269577 28.171329737276984 23.356718978779146 60-61 22.12528129235642 26.9381010304524 27.910790720429517 23.025826956761666 62-63 22.0411705460525 26.785419029359918 27.696805242988116 23.476605181599464 64-65 21.576279898996702 26.657878749410614 27.627987740742082 24.137853610850602 66-67 21.695413235914494 26.44937570810973 27.40845847838347 24.446752577592306 68-69 21.320370809809468 26.62914726648034 27.620439083347435 24.430042840362752 70-71 21.707000157593964 26.28848722374018 27.65558111815914 24.348931500506716 72-73 21.67615737784072 26.546478823968783 27.40545287702917 24.371910921161323 74-75 21.699818367769183 26.801962679593867 27.063839337981854 24.434379614655096 76-77 21.47426629368851 26.355065484483593 27.22796522197996 24.942702999847942 78-79 21.290584315705644 26.33652430282634 27.457348379476965 24.915543001991043 80-81 21.44070520871856 26.448111482484588 27.19367655481158 24.917506753985275 82-83 21.64931570262066 26.190959419752268 27.46366812413757 24.696056753489497 84-85 21.606922824131214 26.462653320579754 26.924024840648205 25.006399014640827 86-87 21.179298024164396 26.544212405701455 27.15664207827434 25.119847491859808 88-89 21.15523850102426 26.087670900621823 27.167616449785374 25.589474148568534 90-91 21.6032026633224 25.7121036239357 27.224381845946027 25.460311866795877 92-93 21.65174795445936 25.909245823176203 27.09377181684211 25.345234405522326 94-95 21.05927491568332 25.64847142542207 27.360174896228216 25.93207876266639 96-97 20.865149547403796 25.53049688607726 27.616883944574845 25.987469621944104 98-99 20.799335633062274 25.71982755433375 27.32859429621812 26.152242516385854 100 20.770587321560786 25.45759228676485 27.41369723600366 26.358123155670704 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1584.0 1 1148.5 2 1780.0 3 2743.5 4 2025.0 5 1518.5 6 2030.0 7 3715.0 8 5845.0 9 6864.5 10 6345.5 11 5702.0 12 6056.5 13 6703.5 14 7572.5 15 8683.5 16 9696.0 17 10658.5 18 11802.5 19 12825.5 20 14158.5 21 15832.5 22 18069.5 23 21728.5 24 26484.5 25 32487.0 26 40429.0 27 49537.5 28 60120.5 29 74157.5 30 90828.5 31 106603.0 32 126455.0 33 147007.0 34 164679.5 35 182921.5 36 203324.0 37 229283.0 38 245473.0 39 249072.0 40 252291.5 41 257196.0 42 266352.0 43 280254.5 44 297375.0 45 323021.0 46 348807.0 47 374578.0 48 411937.5 49 423125.0 50 410731.5 51 408682.0 52 401972.0 53 385231.5 54 360861.0 55 325216.5 56 289408.5 57 253303.0 58 216325.0 59 183549.5 60 146769.0 61 112653.5 62 87211.0 63 66576.5 64 48271.5 65 33370.0 66 23636.5 67 17627.0 68 12887.5 69 9170.5 70 6821.5 71 5016.0 72 3697.0 73 2764.5 74 2031.5 75 1380.0 76 913.0 77 543.5 78 310.5 79 203.0 80 129.0 81 89.5 82 63.0 83 40.0 84 27.0 85 19.5 86 14.0 87 12.0 88 7.5 89 4.5 90 3.0 91 3.0 92 2.5 93 0.5 94 0.0 95 0.5 96 1.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05023933024011594 2 0.0 3 0.0 4 0.01506640279596173 5 0.0040040368462047286 6 0.0 7 2.48228699360401E-4 8 3.5615422082144486E-4 9 0.0019534519384448944 10-11 9.605371410032907E-4 12-13 3.291728404561839E-4 14-15 3.237765643831317E-5 16-17 9.173669324188732E-5 18-19 0.0 20-21 5.396276073052195E-6 22-23 1.079255214610439E-5 24-25 6.475531287662634E-5 26-27 0.0 28-29 9.11970656345821E-4 30-31 8.472153434691946E-4 32-33 0.014008732685643498 34-35 0.02293417331047183 36-37 0.03061847043849816 38-39 0.020613774599059387 40-41 1.5109573004546146E-4 42-43 0.002153114153147826 44-45 0.009912959146196883 46-47 0.014051902894227915 48-49 0.005774015398165849 50-51 0.010528134618524832 52-53 0.015940599519796185 54-55 0.0102043580541417 56-57 0.00314602895058943 58-59 0.008391209293596163 60-61 0.0016782418587192327 62-63 0.0010037073495877083 64-65 0.019351045997965173 66-67 0.045285548805054025 68-69 0.055112167534082074 70-71 0.04864742879856554 72-73 0.04086060242515122 74-75 0.029145287070554905 76-77 0.029458271082791934 78-79 0.012638078563088241 80-81 0.002088358840271199 82-83 0.02774765156763439 84-85 0.04810780119126032 86-87 0.048609654866054175 88-89 0.05992564579124463 90-91 0.034892321088355494 92-93 0.026441752757955753 94-95 0.00784078913414484 96-97 0.003518371999630031 98-99 0.022594207917869542 100 0.019210742820065817 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9265649.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.92624263911195 #Duplication Level Percentage of deduplicated Percentage of total 1 75.309427735643 44.37701611763166 2 14.674540139972287 17.294310258107895 3 4.6772130966159535 8.268317814180715 4 1.9605227794272109 4.62104964000136 5 0.9940193657421617 2.9286913166849393 6 0.5730816112136868 2.026172764463456 7 0.3693900211906405 1.523673621200247 8 0.2515300546279647 1.1857376824029224 9 0.18193916029893845 0.9648891994798378 >10 0.9339084072189721 9.542328159279105 >50 0.04932864426885411 1.9849037334885504 >100 0.022453913683617554 2.3514447728631227 >500 0.0017004803143356778 0.6633157837629055 >1k 8.265160615205157E-4 1.0074524702405194 >5k 2.361474412442424E-5 0.11197626959771337 >10k+ 9.445897649769696E-5 1.1487203966149915 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27411 0.29583464687686745 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22393 0.2416776202077156 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17083 0.1843691683119013 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15664 0.16905453681657917 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.079255214610439E-5 3.237765643831317E-5 2 0.0 0.0 0.0 1.079255214610439E-5 3.237765643831317E-5 3 0.0 1.079255214610439E-5 0.0 1.079255214610439E-5 3.237765643831317E-5 4 0.0 1.079255214610439E-5 0.0 1.079255214610439E-5 3.237765643831317E-5 5 0.0 2.158510429220878E-5 0.0 1.079255214610439E-5 3.237765643831317E-5 6 0.0 2.158510429220878E-5 0.0 1.079255214610439E-5 3.237765643831317E-5 7 0.0 2.158510429220878E-5 0.0 1.079255214610439E-5 3.237765643831317E-5 8 0.0 2.158510429220878E-5 0.0 1.079255214610439E-5 3.237765643831317E-5 9 0.0 2.158510429220878E-5 0.0 1.079255214610439E-5 3.237765643831317E-5 10-11 0.0 2.158510429220878E-5 0.0 1.6188828219156585E-5 3.237765643831317E-5 12-13 0.0 3.7773932511365366E-5 0.0 5.3962760730521954E-5 9.173669324188732E-5 14-15 0.0 4.856648465746976E-5 0.0 6.475531287662634E-5 1.2951062575325268E-4 16-17 0.0 5.3962760730521954E-5 0.0 1.2951062575325268E-4 1.4030317789935707E-4 18-19 0.0 6.475531287662634E-5 0.0 1.8347338648377464E-4 1.4030317789935707E-4 20-21 0.0 6.475531287662634E-5 0.0 5.072499508669064E-4 1.4030317789935707E-4 22-23 0.0 7.015158894967854E-5 0.0 0.0013868429507744142 1.4030317789935707E-4 24-25 0.0 7.554786502273073E-5 0.0 0.0035291645517761356 1.5109573004546146E-4 26-27 0.0 7.554786502273073E-5 0.0 0.0070475365514061665 1.5109573004546146E-4 28-29 0.0 8.094414109578293E-5 1.079255214610439E-5 0.01861175617595702 1.5109573004546146E-4 30-31 0.0 8.634041716883512E-5 1.079255214610439E-5 0.06040591436174628 1.5109573004546146E-4 32-33 0.0 8.634041716883512E-5 1.079255214610439E-5 0.15440364727824246 1.6188828219156586E-4 34-35 0.0 9.173669324188732E-5 1.079255214610439E-5 0.287416456202906 1.7268083433767025E-4 36-37 0.0 1.0252924538799171E-4 1.079255214610439E-5 0.48911846326145103 1.7268083433767025E-4 38-39 0.0 1.0792552146104391E-4 1.079255214610439E-5 0.8181294154354434 1.8347338648377464E-4 40-41 5.396276073052195E-6 1.0792552146104391E-4 1.079255214610439E-5 1.2164555337677911 1.8347338648377464E-4 42-43 1.079255214610439E-5 1.187180736071483E-4 1.079255214610439E-5 1.5920255559000778 1.8347338648377464E-4 44-45 1.6188828219156585E-5 1.187180736071483E-4 1.079255214610439E-5 1.9965735805446547 1.8886966255682682E-4 46-47 2.158510429220878E-5 1.241143496802005E-4 1.079255214610439E-5 2.4606479265510703 1.9426593862987903E-4 48-49 2.158510429220878E-5 1.2951062575325268E-4 1.079255214610439E-5 2.9508078710946206 2.1585104292208782E-4 50-51 2.158510429220878E-5 1.2951062575325268E-4 1.079255214610439E-5 3.4726601450152064 2.428324232873488E-4 52-53 2.158510429220878E-5 1.2951062575325268E-4 1.079255214610439E-5 3.9945825705247415 2.590212515065054E-4 54-55 2.158510429220878E-5 1.5649200611851367E-4 1.079255214610439E-5 4.567073499114849 2.7521007972566196E-4 56-57 2.158510429220878E-5 1.6188828219156586E-4 1.079255214610439E-5 5.168903980714141 2.913989079448185E-4 58-59 2.158510429220878E-5 1.6188828219156586E-4 1.079255214610439E-5 5.847593622421916 3.0219146009092293E-4 60-61 2.158510429220878E-5 1.6188828219156586E-4 1.079255214610439E-5 6.513154124443954 3.345691165292361E-4 62-63 2.158510429220878E-5 1.6188828219156586E-4 1.079255214610439E-5 7.202744243819295 3.5615422082144486E-4 64-65 2.158510429220878E-5 1.6188828219156586E-4 1.079255214610439E-5 7.853686233959435 3.669467729675493E-4 66-67 2.158510429220878E-5 1.7268083433767025E-4 1.079255214610439E-5 8.547328956665638 3.8313560118670583E-4 68-69 2.158510429220878E-5 2.0505849077598343E-4 1.079255214610439E-5 9.312159353327544 3.8853187725975807E-4 70-71 2.158510429220878E-5 2.48228699360401E-4 1.079255214610439E-5 10.102406210293527 4.1011698155196685E-4 72-73 2.158510429220878E-5 2.698138036526098E-4 1.079255214610439E-5 10.837745958216203 4.263058097711234E-4 74-75 2.158510429220878E-5 2.7521007972566196E-4 1.079255214610439E-5 11.536801145823677 4.532871901363844E-4 76-77 2.158510429220878E-5 2.8060635579871414E-4 1.079255214610439E-5 12.273700417531465 4.586834662094366E-4 78-79 2.158510429220878E-5 2.8060635579871414E-4 1.079255214610439E-5 13.074793789404282 4.802685705016454E-4 80-81 2.158510429220878E-5 2.9679518401787075E-4 1.079255214610439E-5 13.865078420302776 4.96457398720802E-4 82-83 2.158510429220878E-5 3.1298401223702735E-4 1.079255214610439E-5 14.662550890930575 5.072499508669064E-4 84-85 2.158510429220878E-5 3.1838028831007953E-4 1.079255214610439E-5 15.447924910602591 5.072499508669064E-4 86-87 2.158510429220878E-5 3.237765643831317E-4 1.079255214610439E-5 16.22288951372969 5.072499508669064E-4 88 2.158510429220878E-5 3.237765643831317E-4 1.079255214610439E-5 16.817580722084337 5.072499508669064E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 10505 0.0 49.92339 2 ACCTGGG 12240 0.0 41.73388 3 CCTGGGG 11160 0.0 33.982445 4 GTACCTG 16485 0.0 32.08701 1 CTGGGGG 7705 0.0 28.47925 5 GTACATG 31960 0.0 27.966688 1 TGGGGGG 18340 0.0 27.59237 6 TACATGG 32700 0.0 26.643904 2 ACATGGG 32420 0.0 25.6712 3 GTATCAA 44940 0.0 24.513477 1 ATAACGC 1155 0.0 24.412365 3 CATGGGG 24410 0.0 24.219107 4 ATGGGGG 17505 0.0 24.211893 5 TAACGCA 1100 0.0 23.924376 4 GGTATCA 33085 0.0 23.150246 1 GTACTAG 3115 0.0 22.037407 1 CAACGCA 51485 0.0 21.036552 5 ATCAACG 51655 0.0 20.91545 3 AGAGTAC 40775 0.0 20.74745 10-11 TCAACGC 52635 0.0 20.588749 4 >>END_MODULE