FastQCFastQC Report
Mon 6 Jun 2016
ERR1211431_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211431_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11215978
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346910.309299822093089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277650.2475486310689982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168930.15061548801183455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA175500.054.225981
GTATCAA322250.041.169241
TACCTGG92050.037.224872
CAACGCA380700.033.9577145
ATCAACG384050.033.612553
TATCAAC404900.032.2814982
AACGCAG407650.032.117086
TCAACGC404800.032.0637664
ACCTGGG108800.031.0662383
ACGCAGA451500.028.5187637
GTACATG396350.028.1234781
CGCAGAG460650.027.8177598
TACATGG402650.027.2483482
GTACTAG47650.026.6952511
ACATGGG401250.026.2083453
CATGGGG273300.024.5799664
GCAGAGT508650.024.4622529
ATGGGGG206200.023.2085365
GTACCTG151800.023.0905531
GCATCGT42150.022.974028
Produced by FastQC (version 0.11.5)