FastQCFastQC Report
Mon 6 Jun 2016
ERR1211431_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211431_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11215978
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312800.2788878508855848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267330.2383474717942564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166730.14865400056954461No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177550.052.9094351
GTATCAA315800.040.3473931
TACCTGG93150.038.6943932
ATCAACG379300.032.6223453
CAACGCA381450.032.4965975
ACCTGGG109850.031.9999073
TATCAAC403850.031.1504152
TCAACGC402650.030.8825514
AACGCAG408900.030.7617236
GTACATG390650.027.4654121
ACGCAGA458200.027.0827487
TACATGG397350.026.4799062
CGCAGAG466200.026.4769068
ACATGGG391900.025.8295943
CATGGGG266950.024.1356284
GCAGAGT509750.023.6709259
GTACCTG156550.023.6363261
TGGGGGG199050.023.3224496
GTACTAG49450.022.914431
GTGGTAT78150.022.5009541
Produced by FastQC (version 0.11.5)