##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211431_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11215978 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.074931405892556 33.0 31.0 34.0 30.0 34.0 2 32.21297001474147 33.0 31.0 34.0 30.0 34.0 3 32.2929339733013 34.0 31.0 34.0 30.0 34.0 4 35.9349536883899 37.0 35.0 37.0 35.0 37.0 5 35.79221794122635 37.0 35.0 37.0 35.0 37.0 6 35.82893279569557 37.0 35.0 37.0 35.0 37.0 7 35.75965377250205 37.0 35.0 37.0 33.0 37.0 8 35.746677463169064 37.0 35.0 37.0 33.0 37.0 9 37.33769627579512 39.0 37.0 39.0 34.0 39.0 10-11 37.31440000149786 39.0 37.0 39.0 34.0 39.0 12-13 37.23298463138926 39.0 37.0 39.0 33.0 39.0 14-15 38.44908682952124 40.0 38.0 41.0 34.0 41.0 16-17 38.44265185791199 40.0 38.0 41.0 34.0 41.0 18-19 38.452725121251135 40.0 38.0 41.0 34.0 41.0 20-21 38.412843177830766 40.0 38.0 41.0 34.0 41.0 22-23 38.30937694421298 40.0 38.0 41.0 34.0 41.0 24-25 38.19777713544018 40.0 38.0 41.0 33.5 41.0 26-27 38.077745337945565 40.0 37.0 41.0 33.0 41.0 28-29 38.011325138119915 40.0 37.0 41.0 33.0 41.0 30-31 38.06132126864015 40.0 37.0 41.0 33.0 41.0 32-33 37.91491816406915 40.0 37.0 41.0 33.0 41.0 34-35 37.81035220468513 40.0 37.0 41.0 33.0 41.0 36-37 37.67505606733537 40.0 37.0 41.0 33.0 41.0 38-39 37.56524112297652 40.0 37.0 41.0 32.0 41.0 40-41 37.50602546652642 40.0 37.0 41.0 32.0 41.0 42-43 37.37865498666278 40.0 36.0 41.0 32.0 41.0 44-45 37.22986421692339 40.0 36.0 41.0 31.0 41.0 46-47 37.018892645830796 39.5 36.0 41.0 31.0 41.0 48-49 36.810515988886564 39.0 36.0 41.0 30.5 41.0 50-51 36.63761893969478 39.0 35.0 41.0 30.0 41.0 52-53 36.43918693492444 39.0 35.0 40.5 30.0 41.0 54-55 36.246670196749676 39.0 35.0 40.0 29.0 41.0 56-57 36.02435427387607 38.0 35.0 40.0 29.0 41.0 58-59 35.77051751528043 38.0 34.5 40.0 28.0 41.0 60-61 35.525025280898376 38.0 34.0 40.0 28.0 41.0 62-63 35.24006693843373 37.0 34.0 40.0 28.0 41.0 64-65 34.899021066196816 37.0 34.0 40.0 27.0 41.0 66-67 34.55480658931393 36.0 33.5 39.0 26.0 41.0 68-69 34.193277082034214 36.0 33.0 39.0 26.0 40.0 70-71 33.835789308787874 35.0 33.0 38.5 26.0 40.0 72-73 33.488797677741516 35.0 32.5 38.0 26.0 40.0 74-75 33.12688960338545 35.0 32.0 37.0 25.0 39.0 76-77 32.09577992217888 34.0 30.5 36.0 24.5 39.0 78-79 32.5754763873467 35.0 32.0 36.0 25.0 39.0 80-81 32.46482660718486 35.0 32.0 36.0 25.0 37.5 82-83 32.19527976071279 35.0 32.0 35.5 25.0 37.0 84-85 31.921001717371414 34.0 31.5 35.0 24.5 37.0 86-87 31.663608291671043 34.0 31.0 35.0 24.0 36.0 88-89 31.428345214300528 34.0 31.0 35.0 23.5 36.0 90-91 31.214746141620463 34.0 31.0 35.0 23.0 35.5 92-93 31.03696079824693 34.0 31.0 35.0 22.5 35.0 94-95 30.874651145000463 34.0 30.5 35.0 20.5 35.0 96-97 30.71022825651049 34.0 30.0 35.0 20.0 35.0 98-99 30.522032229378482 34.0 30.0 35.0 20.0 35.0 100 28.67994846280904 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.23377018049302123 1101 2 -0.24402474516223194 1101 3 -0.28113014919166446 1101 4 -0.10876470616957334 1101 5 -0.1698383985600671 1101 6 -0.25708703496684393 1101 7 -0.25242938425717654 1101 8 -0.27970405658692243 1101 9 -0.4702096775739477 1101 10-11 -0.395103651178907 1101 12-13 -0.3696793171750983 1101 14-15 -0.5494740382373777 1101 16-17 -0.6036508001513354 1101 18-19 -0.5749455634101466 1101 20-21 -0.5804564429615411 1101 22-23 -0.6441929645649793 1101 24-25 -0.6848343148283647 1101 26-27 -0.6687128911564955 1101 28-29 -0.7192586865652331 1101 30-31 -0.5818696964963408 1101 32-33 -0.4644906423898334 1101 34-35 -0.5885240729901753 1101 36-37 -0.6376164247072609 1101 38-39 -0.6413123884800669 1101 40-41 -0.6661651395944261 1101 42-43 -0.6079785966204838 1101 44-45 -0.6513951677608887 1101 46-47 -0.7000511911624088 1101 48-49 -0.6207668501792725 1101 50-51 -0.7642126875964053 1101 52-53 -0.7191750055968029 1101 54-55 -0.7258153563753638 1101 56-57 -0.6860223262330223 1101 58-59 -0.6553017678338406 1101 60-61 -0.668730843245406 1101 62-63 -0.7177818476946598 1101 64-65 -0.6751933168957223 1101 66-67 -0.7230134706651015 1101 68-69 -0.8121165190527435 1101 70-71 -0.8600865447584596 1101 72-73 -0.9020088707995413 1101 74-75 -0.8600813767297311 1101 76-77 -0.7825398026662249 1101 78-79 -0.6665338038104558 1101 80-81 -0.568602054049542 1101 82-83 -0.6616964421910758 1101 84-85 -0.6708038301034165 1101 86-87 -0.7762283042563887 1101 88-89 -0.8173802785461781 1101 90-91 -0.8680185422408897 1101 92-93 -0.8943502251724276 1101 94-95 -1.0329318685295448 1101 96-97 -0.9992238035362782 1101 98-99 -1.0445082178144673 1101 100 -1.077178333228634 1102 1 -0.25474423343638364 1102 2 -0.23091298855717213 1102 3 -0.28313799541494333 1102 4 -0.14763417880615748 1102 5 -0.14168290395353722 1102 6 -0.2643211962554659 1102 7 -0.21726410823136177 1102 8 -0.2666013008935266 1102 9 -0.4653112528620795 1102 10-11 -0.3876865785068375 1102 12-13 -0.4241449587665045 1102 14-15 -0.5413783611180776 1102 16-17 -0.6182234368655344 1102 18-19 -0.610188150837935 1102 20-21 -0.6532566201509127 1102 22-23 -0.5888367149105491 1102 24-25 -0.6627086273837364 1102 26-27 -0.7503865206712561 1102 28-29 -0.7066744476758942 1102 30-31 -0.6416018357944466 1102 32-33 -0.6297271284853494 1102 34-35 -0.6512218519509361 1102 36-37 -0.5994287711292046 1102 38-39 -0.6218569179188336 1102 40-41 -0.7067674484568585 1102 42-43 -0.6197522712683821 1102 44-45 -0.6480003774885645 1102 46-47 -0.7190525978763205 1102 48-49 -0.764405970198716 1102 50-51 -0.7119432684509519 1102 52-53 -0.7268582480852288 1102 54-55 -0.7850879669869215 1102 56-57 -0.7776112635982528 1102 58-59 -0.7791321315092503 1102 60-61 -0.8073590271571831 1102 62-63 -0.8575317702225291 1102 64-65 -0.900838939430912 1102 66-67 -0.8799263636995605 1102 68-69 -0.9311641840233804 1102 70-71 -0.8981043061288645 1102 72-73 -0.9970032066544192 1102 74-75 -0.9378056955214475 1102 76-77 -0.7934423176495251 1102 78-79 -0.6985733525304596 1102 80-81 -0.5627289591889344 1102 82-83 -0.5983772788643904 1102 84-85 -0.7346225440274416 1102 86-87 -0.7673519587808251 1102 88-89 -0.789323633237391 1102 90-91 -0.7897467751433354 1102 92-93 -0.8769855378062346 1102 94-95 -0.9285870320311069 1102 96-97 -0.9470913268325667 1102 98-99 -1.0022683859514956 1102 100 -1.0684210891020491 1103 1 -0.35382766878981897 1103 2 -0.29649522542420215 1103 3 -0.32166063562835845 1103 4 -0.20067269602682813 1103 5 -0.1782450028533944 1103 6 -0.31830899681589386 1103 7 -0.3194042810217468 1103 8 -0.3962951887903259 1103 9 -0.489253959945934 1103 10-11 -0.45774704622012763 1103 12-13 -0.5733284734179307 1103 14-15 -0.7317063998295339 1103 16-17 -0.7624354751000766 1103 18-19 -0.7464628988459623 1103 20-21 -0.7416206643885843 1103 22-23 -0.7928224531669485 1103 24-25 -0.7904472640166063 1103 26-27 -0.8907182763260266 1103 28-29 -0.8559107093498497 1103 30-31 -0.7635053414523085 1103 32-33 -0.7696190378556267 1103 34-35 -0.774843702896618 1103 36-37 -0.6859529044507298 1103 38-39 -0.8379654403854317 1103 40-41 -0.859477152882647 1103 42-43 -0.868925699814298 1103 44-45 -0.8354209128377406 1103 46-47 -0.8908288305993679 1103 48-49 -0.822028197922549 1103 50-51 -0.8059739246482778 1103 52-53 -0.8587568732766258 1103 54-55 -0.9380712096636046 1103 56-57 -1.0236196357852307 1103 58-59 -1.018822764802529 1103 60-61 -0.9738621067526196 1103 62-63 -1.0174300861642465 1103 64-65 -0.9469914954457295 1103 66-67 -0.9713915088910383 1103 68-69 -0.9553106755214529 1103 70-71 -0.9896051627524045 1103 72-73 -1.0412044058026737 1103 74-75 -1.071897571453622 1103 76-77 -0.9290688211530913 1103 78-79 -0.7449945644112894 1103 80-81 -0.7182223759787547 1103 82-83 -0.730828659087738 1103 84-85 -0.7452747280122018 1103 86-87 -0.8316491883966997 1103 88-89 -0.7794517195832782 1103 90-91 -0.9046586289119745 1103 92-93 -0.8594679245308292 1103 94-95 -0.9392357532635209 1103 96-97 -1.0448038096135228 1103 98-99 -1.1396837175800556 1103 100 -1.2298220956790722 1104 1 -0.42827632009607797 1104 2 -0.3831123562465102 1104 3 -0.3924367932653645 1104 4 -0.2587799485028768 1104 5 -0.30270381645513567 1104 6 -0.34906186950065177 1104 7 -0.3096372923228117 1104 8 -0.3683499919207236 1104 9 -0.5057785311305381 1104 10-11 -0.48730376651916174 1104 12-13 -0.5509526700573204 1104 14-15 -0.6802386315068105 1104 16-17 -0.6643735726811713 1104 18-19 -0.7108282704661448 1104 20-21 -0.6870403281421247 1104 22-23 -0.7152265817180421 1104 24-25 -0.8045738402881213 1104 26-27 -0.8095165844200451 1104 28-29 -0.8263591724723369 1104 30-31 -0.802926694025011 1104 32-33 -0.6993738438868746 1104 34-35 -0.8409228159485664 1104 36-37 -0.7805573438397531 1104 38-39 -0.8931055071274514 1104 40-41 -0.9190643721550487 1104 42-43 -0.7640076322756642 1104 44-45 -0.860898289689878 1104 46-47 -0.9044415880085452 1104 48-49 -0.8911435733547464 1104 50-51 -0.9001059521126891 1104 52-53 -0.8339916752987406 1104 54-55 -0.8321251324956123 1104 56-57 -0.906441972083023 1104 58-59 -0.9444380532090335 1104 60-61 -0.9579730881309843 1104 62-63 -0.9592079802242139 1104 64-65 -0.9778025712134948 1104 66-67 -1.0051282751277455 1104 68-69 -0.9826311050639305 1104 70-71 -1.0786111814718495 1104 72-73 -1.0160283972622537 1104 74-75 -0.9572603793488597 1104 76-77 -0.8213684370499372 1104 78-79 -0.8042890853999154 1104 80-81 -0.892640368392648 1104 82-83 -0.9410112652172486 1104 84-85 -0.8334312234576693 1104 86-87 -0.8792139100390592 1104 88-89 -0.9812024794775205 1104 90-91 -0.8805301224551485 1104 92-93 -0.997426999023709 1104 94-95 -0.9955761098308606 1104 96-97 -1.0952235598349027 1104 98-99 -1.0306406088064932 1104 100 -1.1511629254951181 1105 1 -0.3642999020798321 1105 2 -0.37542080093806973 1105 3 -0.40733523876451727 1105 4 -0.26046439524525056 1105 5 -0.3019433674670893 1105 6 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68-69 -0.9326480391137437 1105 70-71 -0.9249620953638455 1105 72-73 -0.9886048578666831 1105 74-75 -0.9721011511063367 1105 76-77 -0.9203312867162801 1105 78-79 -0.8181167912267 1105 80-81 -0.7133270626517714 1105 82-83 -0.7739197317117004 1105 84-85 -0.6210170060440667 1105 86-87 -0.6198130360179981 1105 88-89 -0.6693790430773454 1105 90-91 -0.6777169862442243 1105 92-93 -0.8078229358910178 1105 94-95 -0.8661898607448144 1105 96-97 -0.8984912008549131 1105 98-99 -0.9216948972537935 1105 100 -1.0043652282011237 1106 1 -0.3664908504977511 1106 2 -0.3003137244455125 1106 3 -0.3455940379953475 1106 4 -0.19777105169757192 1106 5 -0.2668987447875182 1106 6 -0.26244649230145 1106 7 -0.28153902808106324 1106 8 -0.27226856263852284 1106 9 -0.4230717836885205 1106 10-11 -0.4015457358261898 1106 12-13 -0.49455898555015665 1106 14-15 -0.6273922285640694 1106 16-17 -0.6555199284875144 1106 18-19 -0.6344761716746987 1106 20-21 -0.647097583821882 1106 22-23 -0.5910777662464781 1106 24-25 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1.0189757238397377 2311 42-43 1.1211495239044567 2311 44-45 1.0868345789637814 2311 46-47 1.106874390150729 2311 48-49 1.11457215945169 2311 50-51 1.1113982498141368 2311 52-53 1.1287535873735877 2311 54-55 1.1247376847857211 2311 56-57 1.1932844000980865 2311 58-59 1.1960640510090954 2311 60-61 1.2015311902670192 2311 62-63 1.1798523432758188 2311 64-65 1.22000777288509 2311 66-67 1.2711667325419498 2311 68-69 1.2255713248956042 2311 70-71 1.2243210519022227 2311 72-73 1.2095494900948864 2311 74-75 1.089949259924758 2311 76-77 0.9595560564912695 2311 78-79 0.9491520221489935 2311 80-81 1.0051567391262353 2311 82-83 0.9984381119795742 2311 84-85 1.003097702585027 2311 86-87 1.0677625449320551 2311 88-89 1.1049181226016138 2311 90-91 1.1493091760696075 2311 92-93 1.1924417417828188 2311 94-95 1.2357037694185813 2311 96-97 1.2622817311317576 2311 98-99 1.3348739344392158 2311 100 1.5070474675239076 2312 1 0.14895401221811255 2312 2 0.13521853143575413 2312 3 0.3288532628978942 2312 4 0.2709113305213151 2312 5 0.31211199493266406 2312 6 0.4276487722845559 2312 7 0.42940915062636265 2312 8 0.24697341298949027 2312 9 0.41904447180453985 2312 10-11 0.2690069725316917 2312 12-13 0.40060225465867916 2312 14-15 0.7929929108914635 2312 16-17 0.8765768296803316 2312 18-19 0.9078686220822973 2312 20-21 0.8712197912405699 2312 22-23 0.47898835558841313 2312 24-25 0.5129174726660821 2312 26-27 0.7927174073750507 2312 28-29 0.8776761178714452 2312 30-31 0.8368789809767492 2312 32-33 0.7140933724731937 2312 34-35 0.8830040130736947 2312 36-37 0.9768705065043335 2312 38-39 0.9553155570068341 2312 40-41 0.9854506058650614 2312 42-43 0.919651118350572 2312 44-45 0.8299705673740831 2312 46-47 0.9659879135334393 2312 48-49 1.0215642583207512 2312 50-51 1.0492670025080955 2312 52-53 1.052167757968519 2312 54-55 1.077888453373312 2312 56-57 1.0641883452245722 2312 58-59 1.1157938465398018 2312 60-61 1.1527109643364284 2312 62-63 1.1421838848655028 2312 64-65 1.1884807016008878 2312 66-67 1.1516156829019124 2312 68-69 1.15612923991916 2312 70-71 0.9915362561600674 2312 72-73 1.0996951578378358 2312 74-75 1.161568598855645 2312 76-77 1.0608550560240566 2312 78-79 0.9660085428435465 2312 80-81 0.8492246380096447 2312 82-83 0.8964432681473937 2312 84-85 0.7856823101712997 2312 86-87 0.9620408095336579 2312 88-89 0.5879900533705005 2312 90-91 0.9619736945242607 2312 92-93 1.0687295656470361 2312 94-95 1.144195307769607 2312 96-97 1.1763896750188003 2312 98-99 1.216815329362305 2312 100 1.3367165979934263 2313 1 -0.3630850981361462 2313 2 -0.034316253664400165 2313 3 0.22199366742123772 2313 4 0.06240476332165201 2313 5 0.20095009529170937 2313 6 0.34395945697314545 2313 7 0.12048292027926522 2313 8 0.08665065496195012 2313 9 0.25969470872466616 2313 10-11 0.3771393198541588 2313 12-13 0.5681293575340192 2313 14-15 0.6109585216930782 2313 16-17 0.7299654652680374 2313 18-19 0.6178968306469272 2313 20-21 0.8342057837487928 2313 22-23 0.8887032484372526 2313 24-25 0.7324433392518017 2313 26-27 0.86920930557433 2313 28-29 0.8077911621997913 2313 30-31 0.7906694077825591 2313 32-33 0.9287497854318474 2313 34-35 0.9404701562377653 2313 36-37 0.8255816408384504 2313 38-39 0.64356931944058 2313 40-41 0.745564499351687 2313 42-43 0.7660020150255491 2313 44-45 0.9667978197483293 2313 46-47 0.9772899325902884 2313 48-49 0.9859576514480253 2313 50-51 0.917725042402715 2313 52-53 0.896586203207228 2313 54-55 0.9699194255286159 2313 56-57 1.0469731954940187 2313 58-59 0.9635553081396253 2313 60-61 0.9791630367807684 2313 62-63 1.0329175643548822 2313 64-65 1.0941322448150785 2313 66-67 1.1269508731902604 2313 68-69 1.1280368471923978 2313 70-71 1.0423198799120073 2313 72-73 1.1015821049281058 2313 74-75 0.7582737334565763 2313 76-77 0.9058710141009563 2313 78-79 0.7594686818537042 2313 80-81 0.8114047388364014 2313 82-83 0.8724270015215936 2313 84-85 0.9021859535291377 2313 86-87 0.9603569858633172 2313 88-89 0.9488941886033935 2313 90-91 1.021702153461682 2313 92-93 0.7917687336160988 2313 94-95 0.8054541942556348 2313 96-97 1.0486784880722801 2313 98-99 1.153606102060479 2313 100 1.1959035476517919 2314 1 -0.27751203132060454 2314 2 -0.11192791210955733 2314 3 0.15071431293731052 2314 4 -0.15702405026897281 2314 5 0.1954460414485908 2314 6 0.229741655200705 2314 7 0.16866879565310455 2314 8 0.16778583953845327 2314 9 0.26243347062386135 2314 10-11 0.4440827970007817 2314 12-13 0.49151893420608417 2314 14-15 0.6155601070698538 2314 16-17 0.7928711977900349 2314 18-19 0.8643400182764935 2314 20-21 0.4679838378023291 2314 22-23 0.6801103895843141 2314 24-25 0.8742043478499966 2314 26-27 0.8896940503629196 2314 28-29 0.8151503008880354 2314 30-31 0.754624929507635 2314 32-33 0.8376004309780001 2314 34-35 0.9253275049631711 2314 36-37 0.8367130409903751 2314 38-39 0.8619162592836958 2314 40-41 0.8736762834336531 2314 42-43 0.6765822603854517 2314 44-45 0.770654837930401 2314 46-47 0.9417085635404803 2314 48-49 0.7743816045984033 2314 50-51 0.9836095384362977 2314 52-53 1.0422681545209613 2314 54-55 1.0955328006964749 2314 56-57 0.9722043137016669 2314 58-59 0.9090710151882249 2314 60-61 1.0202725554083187 2314 62-63 0.736659545924347 2314 64-65 0.8844836009352193 2314 66-67 1.0211718856659289 2314 68-69 1.0359649538633064 2314 70-71 1.0951124257284093 2314 72-73 1.094545821536073 2314 74-75 1.0911719165251839 2314 76-77 0.9281027783565214 2314 78-79 0.9318611176033116 2314 80-81 0.8904887950862843 2314 82-83 0.902450154734062 2314 84-85 0.8019716442889511 2314 86-87 0.9272090526980001 2314 88-89 1.027223338868101 2314 90-91 1.115359527575876 2314 92-93 1.1594940347080716 2314 94-95 1.1829758900443998 2314 96-97 1.257709862871959 2314 98-99 1.2745644607125541 2314 100 1.314829530035766 2315 1 -0.1205288560990283 2315 2 0.10389869434670373 2315 3 0.0797138091381413 2315 4 -0.06851575277082844 2315 5 0.2454856661829652 2315 6 0.38661962162058217 2315 7 0.43124368740917873 2315 8 0.3829657890739 2315 9 0.5912898243619793 2315 10-11 0.5517043111449809 2315 12-13 0.6360857509512172 2315 14-15 0.7334961023642279 2315 16-17 0.739424292901262 2315 18-19 0.8029843987123613 2315 20-21 0.7907208490435025 2315 22-23 0.8843075488846353 2315 24-25 0.8135459512354615 2315 26-27 0.9087379097377095 2315 28-29 0.93289890442869 2315 30-31 0.8776436221660262 2315 32-33 0.9294472246514189 2315 34-35 0.913146625542332 2315 36-37 0.9593888827906767 2315 38-39 0.9694870800008673 2315 40-41 0.9345029731419032 2315 42-43 1.0076799454561467 2315 44-45 0.8366211858699018 2315 46-47 0.8616485150934707 2315 48-49 0.9933542278546312 2315 50-51 0.9538312711094932 2315 52-53 0.9458159194997293 2315 54-55 0.8475552424417714 2315 56-57 0.9749140607194207 2315 58-59 0.9831851738612869 2315 60-61 1.0321974945061356 2315 62-63 1.0051516351519112 2315 64-65 1.0324443764086197 2315 66-67 1.0352635644185355 2315 68-69 0.9212234642522432 2315 70-71 0.5835048356816301 2315 72-73 0.8506354331578407 2315 74-75 1.0246483950907717 2315 76-77 0.9956003259124309 2315 78-79 0.8664498632437585 2315 80-81 0.8844187169403028 2315 82-83 0.8613601548799608 2315 84-85 0.7773048940268659 2315 86-87 0.7660195083901549 2315 88-89 0.8830546749014481 2315 90-91 0.9528460301462758 2315 92-93 1.0017748243818296 2315 94-95 1.0531773364842927 2315 96-97 1.1053205246789695 2315 98-99 0.9728084408431705 2315 100 0.8889999793504657 2316 1 0.48476085320054096 2316 2 0.4754283207339185 2316 3 0.4724831416905815 2316 4 0.2879286201008213 2316 5 0.38754305780020104 2316 6 0.3955609203541428 2316 7 0.4477017274536408 2316 8 0.48513420030602106 2316 9 0.5434041495934636 2316 10-11 0.51107140832287 2316 12-13 0.6431235525180199 2316 14-15 0.791914907534462 2316 16-17 0.7772740383692565 2316 18-19 0.8255847261042888 2316 20-21 0.8571676599081925 2316 22-23 0.8902664452398312 2316 24-25 0.9569172812736895 2316 26-27 0.995099851079317 2316 28-29 0.961813495471759 2316 30-31 0.8131943228241667 2316 32-33 0.7888320030693166 2316 34-35 0.7354503887094097 2316 36-37 0.7148954593777006 2316 38-39 0.6559710239358552 2316 40-41 0.7148779848541622 2316 42-43 0.8697300134435153 2316 44-45 0.8082416751082775 2316 46-47 0.8171146143048205 2316 48-49 0.5832114122467544 2316 50-51 0.67704075576885 2316 52-53 0.8326315188463624 2316 54-55 0.8829664051000208 2316 56-57 0.9237346022348234 2316 58-59 0.8677076754025634 2316 60-61 0.9019567021601134 2316 62-63 0.8629476862251266 2316 64-65 0.9043014955187942 2316 66-67 0.9521567233839008 2316 68-69 0.9456565005849242 2316 70-71 0.8252135629825617 2316 72-73 0.8342577087708563 2316 74-75 0.8717950564353671 2316 76-77 0.6965168219270943 2316 78-79 0.7331601534294805 2316 80-81 0.7037886585149025 2316 82-83 0.677713895017142 2316 84-85 0.7451262650132087 2316 86-87 0.7544462687780076 2316 88-89 0.6694276416848517 2316 90-91 0.7784750331016994 2316 92-93 0.7989229730137062 2316 94-95 0.6995982595430306 2316 96-97 0.8482099564831174 2316 98-99 0.9443465287905113 2316 100 0.6382542071870461 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 4.0 11 11.0 12 42.0 13 286.0 14 1116.0 15 3279.0 16 7914.0 17 16002.0 18 26301.0 19 37689.0 20 51780.0 21 69576.0 22 84039.0 23 86838.0 24 88500.0 25 98594.0 26 116940.0 27 142199.0 28 176066.0 29 215663.0 30 263843.0 31 327913.0 32 412535.0 33 535585.0 34 745766.0 35 1028217.0 36 1568609.0 37 2440309.0 38 2303042.0 39 367269.0 40 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.35412045878185 20.240043717297663 13.24793438266266 24.157901441257827 2 15.461380184590235 21.2140216394861 40.105499493668766 23.219098682254906 3 17.877996718431216 26.996114560505962 30.801320295146823 24.32456842591599 4 12.085098057679646 17.517426095591524 39.241459233530016 31.156016613198815 5 12.432534173932435 38.32584087030325 35.37313072193973 13.868494233824585 6 29.41764864374734 38.36307453527459 18.712964665230263 13.50631215574781 7 26.252824659157426 33.102263694888215 23.274032394031536 17.37087925192282 8 25.58050479639313 35.62577256202072 20.798867467765486 17.99485517382066 9 26.721050366904077 15.290032630933425 20.818068325062512 37.17084867709998 10-11 24.453361359054288 26.368033737856234 29.17993337575827 19.998671527331208 12-13 24.929572385251234 24.42263799624258 28.823879674554497 21.823909943951687 14-15 22.434116408332024 25.859654383160873 26.5171763258577 25.1890528826494 16-17 21.622137898015062 28.434397559907882 27.43813062980732 22.505333912269737 18-19 21.286957766857245 27.946689089440085 29.363894971976585 21.402458171726085 20-21 22.315370606863397 27.235210169637053 28.96653082025723 21.482888403242324 22-23 22.19750797467274 27.103054548745302 28.735079042375038 21.964358434206915 24-25 21.782865047912264 27.108169293791324 29.18689730745077 21.92206835084564 26-27 21.760285428558092 27.3022103782047 28.88460492048998 22.05289927274723 28-29 21.517291394656212 27.658866883954055 29.013224834671735 21.810616886717995 30-31 22.246562679418926 27.24460808721052 28.91383741171959 21.59499182165096 32-33 22.195724988046596 27.384158087055745 28.578473099444302 21.84164382545336 34-35 22.20070567765968 27.40788534365557 28.96360003513674 21.427808943548015 36-37 21.975255647951624 27.189186462994087 28.87382281898073 21.96173507007356 38-39 21.88431995046764 27.29534494007966 29.06576293713406 21.75457217231864 40-41 21.882521367090817 26.74796181121496 29.45719863733502 21.912318184359204 42-43 21.678695714702346 26.924315563973465 28.98966249922988 22.407326222094305 44-45 21.40107697301754 27.377233515697053 29.08533799155825 22.136351519727153 46-47 21.435561515577742 26.97374671854603 28.82701499297708 22.763676772899153 48-49 21.604340656180746 26.9955253311197 28.893851331500432 22.506282681199124 50-51 21.47049593343226 27.10470176400358 28.638901901206186 22.78590040135797 52-53 21.19118924393889 27.397323183564165 28.53531549053589 22.87617208196106 54-55 21.116492150214896 27.183973132868942 28.68816492497967 23.011369791936488 56-57 21.503269835754576 26.987525304542782 28.341859162635124 23.167345697067514 58-59 21.05402655128638 27.135740135776913 28.665416119134157 23.144817193802545 60-61 21.384947711053222 27.39996090337718 28.13593142354281 23.079159962026793 62-63 21.26732734354737 27.13922084160918 28.091242311898917 23.50220950294453 64-65 20.842999611282977 26.917427640357477 28.177980139315682 24.061592609043863 66-67 21.01888943146351 26.852537895646055 27.938422532645866 24.190150140244562 68-69 20.814031565142837 26.933026235655866 28.00851336992659 24.244428829274703 70-71 20.87586414889412 26.61091108707235 28.117441024773253 24.395783739260274 72-73 20.936624068425008 26.744798947398134 27.865349163152885 24.45322782102397 74-75 20.985001148468506 26.779516387515166 27.6712416485203 24.564240815496028 76-77 20.846015188215265 26.660057498160683 27.727542855063586 24.766384458560474 78-79 20.747663559735503 26.628109314600646 27.716588169680822 24.907638955983032 80-81 20.60018096006913 26.748262753021784 27.609147114223642 25.042409172685442 82-83 20.91184622053452 26.308653653338297 27.895724638660234 24.883775487466945 84-85 20.988265622421537 26.413249900764008 27.43424333111818 25.16424114569628 86-87 20.577670688405426 26.616910962810646 27.639405439899868 25.16601290888406 88-89 20.77778256545144 26.146657506449312 27.60754178144817 25.468018146651083 90-91 20.89599775385601 25.82265558721182 27.67203639923426 25.60931025969791 92-93 20.909049196149667 25.862058432793393 27.60335847631876 25.625533894738172 94-95 20.376453596459207 25.622994113122704 27.942176958700554 26.05837533171754 96-97 20.47981710451866 25.515153644223314 27.899647865033533 26.10538138622449 98-99 20.353181151448478 25.712360470807134 27.76191732337955 26.17254105436484 100 20.409567149019196 25.53319618077385 27.759758297596083 26.297478372610865 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1660.0 1 1188.5 2 1299.5 3 1940.5 4 1589.5 5 1268.0 6 1808.0 7 3148.5 8 4909.5 9 6079.0 10 5834.5 11 5420.0 12 5905.5 13 6688.0 14 7845.0 15 9141.0 16 10217.0 17 11421.0 18 12856.0 19 14252.0 20 15946.5 21 18184.0 22 21117.5 23 25126.0 24 30472.5 25 37241.0 26 47047.0 27 59700.0 28 73803.5 29 93441.5 30 119725.5 31 144684.5 32 174106.5 33 206161.0 34 235536.5 35 264697.5 36 294877.5 37 330584.0 38 350061.5 39 351794.5 40 350250.5 41 349692.5 42 356233.5 43 371493.0 44 391606.0 45 420507.0 46 445364.0 47 458119.5 48 471167.0 49 477110.5 50 473289.0 51 466193.5 52 451546.0 53 426737.0 54 396833.0 55 354992.5 56 313084.5 57 267151.0 58 219236.5 59 185148.5 60 148165.0 61 111289.0 62 84662.5 63 64659.5 64 46208.0 65 30478.0 66 21199.5 67 15730.5 68 11649.5 69 8320.0 70 6026.0 71 4344.0 72 3101.5 73 2203.5 74 1512.5 75 1002.0 76 654.5 77 399.0 78 236.5 79 139.5 80 95.5 81 68.5 82 43.5 83 26.0 84 17.0 85 11.5 86 8.5 87 4.5 88 4.5 89 5.0 90 4.5 91 2.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051444466100058324 2 0.0 3 8.915852010408723E-6 4 0.01570973124234017 5 0.00432418822504823 6 0.0 7 2.0506459623940063E-4 8 4.4579260052043614E-4 9 0.0021398044824980935 10-11 8.068846069419894E-4 12-13 2.6301763430705734E-4 14-15 2.2289630026021807E-5 16-17 7.132681608326978E-5 18-19 0.0 20-21 2.2289630026021807E-5 22-23 4.4579260052043614E-6 24-25 7.578474208847414E-5 26-27 1.3373778015613083E-5 28-29 9.807437211449596E-4 30-31 7.444736428691283E-4 32-33 0.014265363216653957 34-35 0.023564596863510253 36-37 0.030666072989800803 38-39 0.020493085845924446 40-41 1.3373778015613084E-4 42-43 0.0018812447741962406 44-45 0.009981296325652565 46-47 0.014510549146940195 48-49 0.005554575802484634 50-51 0.010489499890245862 52-53 0.016035159840720088 54-55 0.009673699431293464 56-57 0.0031517536856794834 58-59 0.007939566215268968 60-61 0.0015647320278267308 62-63 9.539961651137332E-4 64-65 0.019302819602534885 66-67 0.04481553013031944 68-69 0.054217296075295435 70-71 0.04797619966800933 72-73 0.04031302486506304 74-75 0.02870458554751088 76-77 0.029168209852052138 78-79 0.012736294596868859 80-81 0.0021754678905397283 82-83 0.02754552478615775 84-85 0.04750365951145767 86-87 0.048181264264248735 88-89 0.0591745097930827 90-91 0.034540010688323394 92-93 0.02653803350898156 94-95 0.0077032961369931366 96-97 0.0034950139880802187 98-99 0.022771086034583877 100 0.019507884198774285 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1215978E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.897403375975664 #Duplication Level Percentage of deduplicated Percentage of total 1 73.47273662410568 43.273534060867725 2 15.67505966450535 18.464406240057148 3 5.318746306539595 9.397810400122298 4 2.2253786282737624 5.242760909348608 5 1.1205700045027938 3.2999331783109946 6 0.6038607083053483 2.1339496631977486 7 0.3625841069767101 1.4948683684427646 8 0.24854090139545246 1.1710730979933244 9 0.16535717231913252 0.8765197271271598 >10 0.7391392969077634 7.3966987106413455 >50 0.03882763624478509 1.5750364899813425 >100 0.02624123273072084 3.010112955547202 >500 0.002080896922427523 0.8149655564028335 >1k 7.748644429119117E-4 0.8023432845679305 >5k 4.0782331042995944E-5 0.14901842986330024 >10k+ 6.117349656449392E-5 0.8969689275282433 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31280 0.2788878508855848 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26733 0.2383474717942564 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16673 0.14865400056954461 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.915852010408723E-6 2 0.0 0.0 0.0 0.0 8.915852010408723E-6 3 0.0 0.0 0.0 4.4579260052043614E-5 8.915852010408723E-6 4 0.0 0.0 0.0 4.4579260052043614E-5 8.915852010408723E-6 5 0.0 0.0 0.0 5.349511206245233E-5 8.915852010408723E-6 6 0.0 0.0 0.0 5.349511206245233E-5 8.02426680936785E-5 7 0.0 0.0 0.0 5.349511206245233E-5 8.02426680936785E-5 8 0.0 0.0 0.0 5.349511206245233E-5 8.02426680936785E-5 9 0.0 0.0 0.0 7.132681608326978E-5 8.915852010408723E-5 10-11 0.0 0.0 0.0 7.578474208847414E-5 8.915852010408723E-5 12-13 0.0 0.0 0.0 8.470059409888287E-5 1.2036400214051775E-4 14-15 0.0 2.2289630026021807E-5 0.0 1.025322981197003E-4 1.3819570616133522E-4 16-17 0.0 3.566340804163489E-5 0.0 1.515694841769483E-4 1.4265363216653957E-4 18-19 0.0 3.566340804163489E-5 0.0 2.0952252224460497E-4 1.4265363216653957E-4 20-21 0.0 3.566340804163489E-5 0.0 5.037456385880928E-4 1.4711155817174391E-4 22-23 0.0 3.566340804163489E-5 0.0 0.0013774991356081475 1.5602741018215266E-4 24-25 0.0 4.012133404683925E-5 0.0 0.0035351353221270585 1.6940118819776573E-4 26-27 0.0 4.4579260052043614E-5 0.0 0.007382325464618422 2.050645962394006E-4 28-29 0.0 4.4579260052043614E-5 0.0 0.022133602615839652 2.050645962394006E-4 30-31 0.0 4.4579260052043614E-5 0.0 0.07578474208847413 2.050645962394006E-4 32-33 0.0 4.4579260052043614E-5 0.0 0.1964607990493562 2.1398044824980932E-4 34-35 0.0 4.4579260052043614E-5 0.0 0.3824053506524353 2.1398044824980932E-4 36-37 0.0 4.9037186057247976E-5 0.0 0.6749255392619351 2.1398044824980932E-4 38-39 0.0 6.241096407286106E-5 0.0 1.0999664942281449 2.273542262654224E-4 40-41 0.0 7.132681608326978E-5 0.0 1.5594226379545324 2.407280042810355E-4 42-43 0.0 7.132681608326978E-5 0.0 2.0023487920536223 2.4518593028623984E-4 44-45 8.915852010408723E-6 7.132681608326978E-5 0.0 2.519356760507198 2.4964385629144424E-4 46-47 8.915852010408723E-6 7.132681608326978E-5 0.0 3.119393600807705 2.5855970830185294E-4 48-49 8.915852010408723E-6 7.578474208847414E-5 0.0 3.7438063805046693 2.8530726433307913E-4 50-51 8.915852010408723E-6 8.02426680936785E-5 0.0 4.378378773567494 2.8530726433307913E-4 52-53 8.915852010408723E-6 8.02426680936785E-5 0.0 4.9820176180802065 3.031389683538966E-4 54-55 8.915852010408723E-6 8.02426680936785E-5 0.0 5.630806337173628 3.2542859837991837E-4 56-57 8.915852010408723E-6 8.02426680936785E-5 0.0 6.344836803353216 3.343444503903271E-4 58-59 8.915852010408723E-6 8.02426680936785E-5 0.0 7.122080660286602 3.4771822840594016E-4 60-61 8.915852010408723E-6 8.470059409888287E-5 0.0 7.892655459916202 3.4771822840594016E-4 62-63 8.915852010408723E-6 8.915852010408723E-5 0.0 8.662864709613373 3.4771822840594016E-4 64-65 8.915852010408723E-6 8.915852010408723E-5 0.0 9.41089131950865 3.4771822840594016E-4 66-67 8.915852010408723E-6 9.807437211449595E-5 0.0 10.213402701039534 3.4771822840594016E-4 68-69 8.915852010408723E-6 9.807437211449595E-5 0.0 11.085190252691294 3.4771822840594016E-4 70-71 8.915852010408723E-6 1.025322981197003E-4 0.0 11.979307555703123 3.70007858431962E-4 72-73 8.915852010408723E-6 1.0699022412490466E-4 0.0 12.831810119456367 4.5470845253084484E-4 74-75 8.915852010408723E-6 1.0699022412490466E-4 0.0 13.643531576114004 4.5470845253084484E-4 76-77 8.915852010408723E-6 1.0699022412490466E-4 0.0 14.498463709540086 4.5470845253084484E-4 78-79 8.915852010408723E-6 1.0699022412490466E-4 0.0 15.406142023459747 4.5470845253084484E-4 80-81 8.915852010408723E-6 1.0699022412490466E-4 0.0 16.32874547364483 4.591663785360492E-4 82-83 8.915852010408723E-6 1.0699022412490466E-4 0.0 17.23834961159874 4.725401565516623E-4 84-85 8.915852010408723E-6 1.0699022412490466E-4 0.0 18.095461670841367 4.725401565516623E-4 86-87 8.915852010408723E-6 1.0699022412490466E-4 0.0 18.95527523324315 4.725401565516623E-4 88 8.915852010408723E-6 1.0699022412490466E-4 0.0 19.608713569159995 4.725401565516623E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17755 0.0 52.909435 1 GTATCAA 31580 0.0 40.347393 1 TACCTGG 9315 0.0 38.694393 2 ATCAACG 37930 0.0 32.622345 3 CAACGCA 38145 0.0 32.496597 5 ACCTGGG 10985 0.0 31.999907 3 TATCAAC 40385 0.0 31.150415 2 TCAACGC 40265 0.0 30.882551 4 AACGCAG 40890 0.0 30.761723 6 GTACATG 39065 0.0 27.465412 1 ACGCAGA 45820 0.0 27.082748 7 TACATGG 39735 0.0 26.479906 2 CGCAGAG 46620 0.0 26.476906 8 ACATGGG 39190 0.0 25.829594 3 CATGGGG 26695 0.0 24.135628 4 GCAGAGT 50975 0.0 23.670925 9 GTACCTG 15655 0.0 23.636326 1 TGGGGGG 19905 0.0 23.322449 6 GTACTAG 4945 0.0 22.91443 1 GTGGTAT 7815 0.0 22.500954 1 >>END_MODULE