FastQCFastQC Report
Mon 6 Jun 2016
ERR1211430_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211430_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10234417
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338490.33073696332678254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249200.24349213052389795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168250.16439627191270398No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154700.052.2314531
TACCTGG99350.048.9382482
GTATCAA291000.042.4843671
ACCTGGG120200.039.810383
ATCAACG359200.033.631783
CAACGCA360550.033.440345
TATCAAC368700.033.126852
GTACATG386900.032.8428231
TCAACGC375450.032.1756744
AACGCAG387150.031.664986
TACATGG396750.031.4254112
GTACCTG164600.030.3126871
ACATGGG398200.030.2552223
CCTGGGG117200.029.7192084
CATGGGG316100.028.9972464
TGGGGGG266200.028.5177566
ACGCAGA426900.028.3094257
ATGGGGG251600.028.1920975
CGCAGAG435450.027.720518
TAACGCA13100.026.193784
Produced by FastQC (version 0.11.5)